##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049403_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7102389 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.996678723173286 33.0 31.0 34.0 30.0 34.0 2 32.18207746717337 33.0 31.0 34.0 30.0 34.0 3 32.242217231413264 34.0 31.0 34.0 30.0 34.0 4 35.905669627501396 37.0 35.0 37.0 35.0 37.0 5 35.795819969872106 37.0 35.0 37.0 35.0 37.0 6 35.83630212313068 37.0 35.0 37.0 35.0 37.0 7 35.760755007927614 37.0 35.0 37.0 33.0 37.0 8 35.752539040032865 37.0 35.0 37.0 33.0 37.0 9 37.33090020836651 39.0 37.0 39.0 34.0 39.0 10-11 37.32280236973784 39.0 37.0 39.0 34.0 39.0 12-13 37.30739347000002 39.0 37.0 39.0 34.0 39.0 14-15 38.482330593269396 40.0 38.0 41.0 34.0 41.0 16-17 38.47520362514641 40.0 38.0 41.0 34.0 41.0 18-19 38.54118226979682 40.0 38.0 41.0 34.0 41.0 20-21 38.54382736569343 40.0 38.0 41.0 34.0 41.0 22-23 38.45291577242531 40.0 38.0 41.0 34.0 41.0 24-25 38.35737024542024 40.0 38.0 41.0 34.0 41.0 26-27 38.17521146757802 40.0 38.0 41.0 33.5 41.0 28-29 37.99273040381202 40.0 37.5 41.0 33.0 41.0 30-31 37.774524036912084 40.0 37.0 41.0 33.0 41.0 32-33 37.49869811411344 39.5 36.5 41.0 32.5 41.0 34-35 37.310380211503485 39.0 36.0 41.0 32.0 41.0 36-37 37.11577365024642 39.0 36.0 41.0 31.5 41.0 38-39 37.247386477986495 39.5 36.0 41.0 32.0 41.0 40-41 37.13682860795149 40.0 36.0 41.0 32.0 41.0 42-43 36.91702876313872 40.0 36.0 41.0 31.0 41.0 44-45 36.6199218319357 39.0 36.0 41.0 30.5 41.0 46-47 36.208760671937284 39.0 35.5 41.0 30.0 41.0 48-49 35.86566660879882 39.0 35.0 41.0 28.5 41.0 50-51 35.60773522824503 39.0 35.0 40.0 28.0 41.0 52-53 35.4153094824854 39.0 35.0 40.0 27.0 41.0 54-55 35.30471359707276 39.0 35.0 40.0 27.0 41.0 56-57 35.21085771280622 38.5 35.0 40.0 27.0 41.0 58-59 34.95988779268497 38.0 34.5 40.0 26.0 41.0 60-61 34.711022094115094 38.0 34.0 40.0 26.0 41.0 62-63 34.424947014307435 37.5 34.0 40.0 26.0 41.0 64-65 34.16818073467955 37.0 34.0 40.0 26.0 41.0 66-67 33.815990929249296 36.5 33.5 39.5 25.0 41.0 68-69 33.483676056042555 36.0 33.0 39.0 24.0 41.0 70-71 33.144138120286 36.0 33.0 39.0 23.5 40.0 72-73 32.767466130058494 35.0 33.0 38.5 22.5 40.0 74-75 32.392117426967175 35.0 32.0 37.5 22.0 40.0 76-77 30.420662047657487 33.5 30.0 35.5 19.5 38.5 78-79 31.735200437486597 35.0 32.0 36.0 22.0 39.0 80-81 31.75505558763396 35.0 32.0 36.0 21.5 39.0 82-83 31.5031255680307 35.0 32.0 36.0 20.0 37.5 84-85 31.25548417581746 35.0 32.0 35.5 20.0 37.0 86-87 31.008736848967295 35.0 32.0 35.0 19.5 37.0 88-89 30.749643394638056 35.0 32.0 35.0 18.0 36.0 90-91 30.494230673650797 34.5 31.5 35.0 16.0 36.0 92-93 30.26080133036926 34.0 31.0 35.0 9.5 36.0 94-95 30.001408892134748 34.0 31.0 35.0 4.5 35.5 96-97 29.764931137959355 34.0 31.0 35.0 2.0 35.0 98-99 29.48339959976847 34.0 31.0 35.0 2.0 35.0 100-101 28.876472550292583 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4052903176015974 1101 2 0.4460200393765419 1101 3 0.4059834646590872 1101 4 0.33593324978532735 1101 5 0.3756385236576776 1101 6 0.3634957768908933 1101 7 0.3829068109879401 1101 8 0.3628695890700513 1101 9 0.4672194548329074 1101 10-11 0.41557606085818577 1101 12-13 0.3764406426935949 1101 14-15 0.5305669226870933 1101 16-17 0.5247672263682901 1101 18-19 0.4632384199171895 1101 20-21 0.4281479773117951 1101 22-23 0.40969888597148696 1101 24-25 0.3830375425417145 1101 26-27 0.37209317560258626 1101 28-29 0.38667539174229404 1101 30-31 0.3314260275949863 1101 32-33 0.302506561671926 1101 34-35 0.20735661471162814 1101 36-37 0.2864265499911198 1101 38-39 0.46096453186438424 1101 40-41 0.4802177506255134 1101 42-43 0.4287519881446755 1101 44-45 0.3979260925250756 1101 46-47 0.39374571591263674 1101 48-49 0.22298498466633276 1101 50-51 0.27615615745666133 1101 52-53 0.32774534523955623 1101 54-55 0.37137376839934433 1101 56-57 0.27505807618027234 1101 58-59 0.20408836084123294 1101 60-61 0.2410937456292359 1101 62-63 0.19898688963234434 1101 64-65 0.09301795476334718 1101 66-67 0.12715698571504674 1101 68-69 0.0715929291396904 1101 70-71 0.07699118432316965 1101 72-73 0.07042681083154179 1101 74-75 0.04041989560717951 1101 76-77 0.02478325078439525 1101 78-79 0.12604203088670474 1101 80-81 0.11400728981206498 1101 82-83 0.05008290454581754 1101 84-85 0.007945559817006398 1101 86-87 0.02136475234980395 1101 88-89 -0.13597420168768082 1101 90-91 -0.10387826104372522 1101 92-93 -0.1589965913183491 1101 94-95 -0.13554151311476303 1101 96-97 -0.1852809908958939 1101 98-99 -0.2323132569737183 1101 100-101 -0.23731437913574993 1102 1 0.41662933326132645 1102 2 0.41546222680444345 1102 3 0.38882441136406243 1102 4 0.33023870872690253 1102 5 0.334642116843213 1102 6 0.3560837763234588 1102 7 0.36076526784948726 1102 8 0.3601171872986981 1102 9 0.50074930527731 1102 10-11 0.4236659753840115 1102 12-13 0.3812799748293827 1102 14-15 0.47714005372642276 1102 16-17 0.45265040311575433 1102 18-19 0.48272958564866997 1102 20-21 0.49345344935679236 1102 22-23 0.4746581086994297 1102 24-25 0.4462421262318301 1102 26-27 0.46387796959689354 1102 28-29 0.44149772931259434 1102 30-31 0.37502408988408575 1102 32-33 0.4071658414282169 1102 34-35 0.34112057198991863 1102 36-37 0.2920869993700492 1102 38-39 0.45142188108817294 1102 40-41 0.5111277717220162 1102 42-43 0.5435284295635583 1102 44-45 0.5167130007276413 1102 46-47 0.4635948727645598 1102 48-49 0.21874969738524896 1102 50-51 0.2595363953350045 1102 52-53 0.3551567760930965 1102 54-55 0.31928368751706415 1102 56-57 0.29442982987928445 1102 58-59 0.27597464366886015 1102 60-61 0.3269611948549098 1102 62-63 0.2801638045974215 1102 64-65 0.26514203617090715 1102 66-67 0.283767883088494 1102 68-69 0.26485664343500304 1102 70-71 0.25512761598854894 1102 72-73 0.22751525378637183 1102 74-75 0.22779003359758576 1102 76-77 0.24972004957341198 1102 78-79 0.3307143570912068 1102 80-81 0.3043364989865083 1102 82-83 0.35880922367561396 1102 84-85 0.3564289415475308 1102 86-87 0.2377103453564544 1102 88-89 0.22896806635473155 1102 90-91 0.27124599070828737 1102 92-93 0.20536676764641015 1102 94-95 0.2279893571175151 1102 96-97 0.16983093224741808 1102 98-99 0.18641975629434882 1102 100-101 0.12036424183758143 1103 1 0.33773905339207744 1103 2 0.34785605226262817 1103 3 0.3253062728273548 1103 4 0.2674812447886268 1103 5 0.2902768465773775 1103 6 0.33590311584177357 1103 7 0.31841970811628784 1103 8 0.3210778244358963 1103 9 0.3767409537512947 1103 10-11 0.36315708469143004 1103 12-13 0.3614914826226965 1103 14-15 0.47687366629053685 1103 16-17 0.4757048584093795 1103 18-19 0.4223599152757487 1103 20-21 0.3747225278634687 1103 22-23 0.36398670611502837 1103 24-25 0.39901582336943164 1103 26-27 0.3364675458511286 1103 28-29 0.3856333643597978 1103 30-31 0.35937562436588166 1103 32-33 0.3078844515107946 1103 34-35 0.2949302837606851 1103 36-37 0.26469469246733723 1103 38-39 0.3786115375560044 1103 40-41 0.44685314916506513 1103 42-43 0.41490037829881743 1103 44-45 0.4834522017875642 1103 46-47 0.5271469274706604 1103 48-49 0.44882022820240763 1103 50-51 0.44799015664347763 1103 52-53 0.4199837189710749 1103 54-55 0.40281296918212206 1103 56-57 0.3696823492527699 1103 58-59 0.33373586960345847 1103 60-61 0.351136333509082 1103 62-63 0.3631796240300602 1103 64-65 0.4187964954857861 1103 66-67 0.3406281135491298 1103 68-69 0.31304932960620135 1103 70-71 0.3061113965461715 1103 72-73 0.3001863769988873 1103 74-75 0.3198256386969689 1103 76-77 0.3012521609369685 1103 78-79 0.4207993750334431 1103 80-81 0.3263449923149153 1103 82-83 0.4032780625997354 1103 84-85 0.43348395981781707 1103 86-87 0.37212850579845025 1103 88-89 0.4172144787012755 1103 90-91 0.4021178849448681 1103 92-93 0.41356905193481097 1103 94-95 0.45817597541616095 1103 96-97 0.4308098343794313 1103 98-99 0.4628326319516418 1103 100-101 0.4003510774960155 1104 1 0.3249486891821576 1104 2 0.3177097947346681 1104 3 0.32504199451891225 1104 4 0.22426446157184898 1104 5 0.2791842168096679 1104 6 0.288563326293783 1104 7 0.3550877719136949 1104 8 0.29625684100292204 1104 9 0.339468888277338 1104 10-11 0.3210210188645135 1104 12-13 0.30780746947495885 1104 14-15 0.3308971822067974 1104 16-17 0.38320855345250493 1104 18-19 0.33363145131068705 1104 20-21 0.345651472735625 1104 22-23 0.2848929292294571 1104 24-25 0.30647539849461936 1104 26-27 0.3575961333519473 1104 28-29 0.33281917539418515 1104 30-31 0.3476904641002747 1104 32-33 0.38435728467132435 1104 34-35 0.2935237407768554 1104 36-37 0.2977177892443734 1104 38-39 0.34366790075116427 1104 40-41 0.3842361762326334 1104 42-43 0.35418695922577825 1104 44-45 0.3571302587463734 1104 46-47 0.5145447347107321 1104 48-49 0.435999200649519 1104 50-51 0.43397656770875415 1104 52-53 0.4031440286928998 1104 54-55 0.39808816717025053 1104 56-57 0.435802884868572 1104 58-59 0.45682051366602394 1104 60-61 0.5254916312082258 1104 62-63 0.4013115867046153 1104 64-65 0.43265500290896597 1104 66-67 0.433147802062507 1104 68-69 0.3794663510705405 1104 70-71 0.2843927051429418 1104 72-73 0.3371070273264749 1104 74-75 0.3172391085079127 1104 76-77 0.23962612136140038 1104 78-79 0.3542160214784822 1104 80-81 0.32943029817624847 1104 82-83 0.3568674385234978 1104 84-85 0.33646558387200187 1104 86-87 0.3288261299408717 1104 88-89 0.3066585677352691 1104 90-91 0.2529705023589983 1104 92-93 0.15878843822675037 1104 94-95 0.12618175512541185 1104 96-97 0.05972977878833419 1104 98-99 0.05523429968453897 1104 100-101 0.08433554728490478 1105 1 0.29046926909378357 1105 2 0.3055286716523966 1105 3 0.2737483837059145 1105 4 0.25537906515504716 1105 5 0.2683724398373215 1105 6 0.2644135056065551 1105 7 0.22086825031022528 1105 8 0.2547528289036123 1105 9 0.3205501118053107 1105 10-11 0.3223816571339455 1105 12-13 0.2926337709724791 1105 14-15 0.36491287159659436 1105 16-17 0.31892654927427344 1105 18-19 0.33513269446949323 1105 20-21 0.37187671936229094 1105 22-23 0.24337438496650066 1105 24-25 0.3226530766329816 1105 26-27 0.35554266797517187 1105 28-29 0.29136397303238226 1105 30-31 0.36527457305522404 1105 32-33 0.4459519079617351 1105 34-35 0.4370844455008509 1105 36-37 0.38457865093866417 1105 38-39 0.45720320760705846 1105 40-41 0.4830045514947301 1105 42-43 0.4555176738415838 1105 44-45 0.45987571100029356 1105 46-47 0.4559147491856095 1105 48-49 0.4398524964525663 1105 50-51 0.4003029093081736 1105 52-53 0.38747127163019 1105 54-55 0.42689179390943366 1105 56-57 0.4885642633044469 1105 58-59 0.4654528289204052 1105 60-61 0.5372912324807899 1105 62-63 0.4753957166653109 1105 64-65 0.463970918015157 1105 66-67 0.4870454963943942 1105 68-69 0.47564645441885745 1105 70-71 0.4168183925085529 1105 72-73 0.464319028869447 1105 74-75 0.39532323083015797 1105 76-77 0.412967564189465 1105 78-79 0.48035308212400807 1105 80-81 0.5065456541474163 1105 82-83 0.5205151452678685 1105 84-85 0.5183431355938986 1105 86-87 0.47323896178406955 1105 88-89 0.5226033875249847 1105 90-91 0.5688942018162848 1105 92-93 0.5328977178057208 1105 94-95 0.499256252992371 1105 96-97 0.418236880643331 1105 98-99 0.4205348729507037 1105 100-101 0.5646064642434219 1106 1 0.2198709070230862 1106 2 0.1809444640809872 1106 3 0.19852079779203535 1106 4 0.19605903330764818 1106 5 0.19974694497278733 1106 6 0.23013483999066153 1106 7 0.22417686108113344 1106 8 0.23234040484359042 1106 9 0.24755079657472123 1106 10-11 0.2541374812464028 1106 12-13 0.21502591214883893 1106 14-15 0.20804932487314431 1106 16-17 0.27605194903831176 1106 18-19 0.23074648123668595 1106 20-21 0.22493269102761815 1106 22-23 0.2321123601413433 1106 24-25 0.24309822718405627 1106 26-27 0.17971233252985996 1106 28-29 0.23159661734499082 1106 30-31 0.3370450859720435 1106 32-33 0.3023223362700662 1106 34-35 0.25964543494947634 1106 36-37 0.285446740019367 1106 38-39 0.3435061917451847 1106 40-41 0.3366785330922326 1106 42-43 0.3182057514032408 1106 44-45 0.35558630808391456 1106 46-47 0.41250507016372495 1106 48-49 0.37016081768086906 1106 50-51 0.5146617982470687 1106 52-53 0.39264718958684597 1106 54-55 0.40013277290731963 1106 56-57 0.2992350354799598 1106 58-59 0.3955705002122869 1106 60-61 0.42246933518887175 1106 62-63 0.38444993709075703 1106 64-65 0.3836572132353595 1106 66-67 0.37658863155623123 1106 68-69 0.40340592765458183 1106 70-71 0.4114079328143916 1106 72-73 0.38465482722491373 1106 74-75 0.4136627632077676 1106 76-77 0.3968947782676686 1106 78-79 0.4354767196441749 1106 80-81 0.5173782366236992 1106 82-83 0.46118886636108414 1106 84-85 0.39547859828168086 1106 86-87 0.3766091891930792 1106 88-89 0.37111150950456917 1106 90-91 0.42845931325427244 1106 92-93 0.38436903784261034 1106 94-95 0.49232670713715976 1106 96-97 0.49248862620927625 1106 98-99 0.455063354077744 1106 100-101 0.4942060881054928 1107 1 0.23287910802022083 1107 2 0.2142181907972045 1107 3 0.19970314546987566 1107 4 0.1554613644566487 1107 5 0.13538282711655825 1107 6 0.1552087244200706 1107 7 0.18419375086043743 1107 8 0.19376908990052044 1107 9 0.22200162624001507 1107 10-11 0.18690585324456777 1107 12-13 0.17906450331160784 1107 14-15 0.2027143989603175 1107 16-17 0.2500222996850425 1107 18-19 0.20491984974876232 1107 20-21 0.1975832352406428 1107 22-23 0.19372930251645926 1107 24-25 0.2199690439150217 1107 26-27 0.17179962094716217 1107 28-29 0.21621984054645083 1107 30-31 0.2339409837504789 1107 32-33 0.25266751212964067 1107 34-35 0.27894948276310316 1107 36-37 0.21994261590391773 1107 38-39 0.2991753948494207 1107 40-41 0.28974606443360784 1107 42-43 0.29389278153510645 1107 44-45 0.21314855043345204 1107 46-47 0.20636215561254545 1107 48-49 0.15931007767058247 1107 50-51 0.24060544838221887 1107 52-53 0.21783665559890153 1107 54-55 0.25585799553721955 1107 56-57 0.323893912736672 1107 58-59 0.3385431169650559 1107 60-61 0.3357329159319917 1107 62-63 0.3122918140144577 1107 64-65 0.3342942580275192 1107 66-67 0.4679434125030255 1107 68-69 0.3421072133929357 1107 70-71 0.40091149420950245 1107 72-73 0.35897483573887 1107 74-75 0.38374273492395616 1107 76-77 0.351460468012629 1107 78-79 0.46737061370154365 1107 80-81 0.4651843848538988 1107 82-83 0.5508516708516744 1107 84-85 0.5027703698503494 1107 86-87 0.531138035126979 1107 88-89 0.5303609083882463 1107 90-91 0.6125842166701645 1107 92-93 0.6204978090045934 1107 94-95 0.6283731385827203 1107 96-97 0.6675009990754788 1107 98-99 0.6737231012227056 1107 100-101 0.7456246695214794 1108 1 0.09413697966372325 1108 2 0.11615169248420187 1108 3 0.09655218256731501 1108 4 0.10146972528266218 1108 5 0.08374689123517953 1108 6 0.12000847161221628 1108 7 0.14621519445849174 1108 8 0.09732100218465689 1108 9 0.16559532878653727 1108 10-11 0.07100599143603148 1108 12-13 0.1004677810771426 1108 14-15 0.14704289801940007 1108 16-17 0.14246686535614828 1108 18-19 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70-71 -1.0621976004766935 2316 72-73 -1.0671975831940657 2316 74-75 -0.924012826683807 2316 76-77 -0.8445288464322864 2316 78-79 -0.82669221640354 2316 80-81 -0.727526936128509 2316 82-83 -0.832677335415255 2316 84-85 -1.0088888488740757 2316 86-87 -1.1243736108840992 2316 88-89 -0.8954444343755164 2316 90-91 -0.8268592189189441 2316 92-93 -0.8511322847046117 2316 94-95 -1.0480509833274176 2316 96-97 -1.0082553531313927 2316 98-99 -1.0995804754702334 2316 100-101 -1.3835113216520263 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 12.0 4 70.0 5 132.0 6 335.0 7 926.0 8 2556.0 9 4907.0 10 8379.0 11 12830.0 12 18455.0 13 26225.0 14 33816.0 15 45984.0 16 60671.0 17 81747.0 18 66719.0 19 35202.0 20 26982.0 21 26111.0 22 26509.0 23 28653.0 24 34361.0 25 43376.0 26 54658.0 27 69278.0 28 88410.0 29 111686.0 30 141612.0 31 178451.0 32 230474.0 33 304673.0 34 425776.0 35 639092.0 36 1004406.0 37 1533492.0 38 1462153.0 39 273163.0 40 102.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.600524763418356 22.714316665112417 16.544965654845637 22.140192916623587 2 14.686903269754383 21.91408182707273 39.06999278819686 24.329022114976024 3 16.55414333704242 27.38994919871215 31.67782231840909 24.378085145836344 4 11.629099175726196 18.84679951334575 39.07991033419394 30.444190976734113 5 11.819931334184899 38.85222431734059 35.55842507924278 13.769419269231722 6 28.35695184172833 39.85095467378101 17.733896026748635 14.058197457742025 7 25.828168521887495 34.13854127111314 22.422919949892915 17.610370257106446 8 26.07055175378313 34.706533252402814 20.008408438343775 19.21450655547028 9 25.456308243672087 16.11949570151193 22.116723954966652 36.30747209984933 10-11 23.586614161310425 27.596287529550807 28.488459477669934 20.328638831468833 12-13 24.657407528649866 25.680922292484965 28.55442724976061 21.107242929104558 14-15 21.963260350019162 26.932563485289524 27.46566138494364 23.63851477974767 16-17 19.952687750558297 30.350893199457254 28.156293607686088 21.540125442298358 18-19 19.959054602147404 28.1473245855213 31.908300387243134 19.98532042508816 20-21 21.476760669276505 27.10388637235306 31.707971270378447 19.711381687991985 22-23 20.90450832811326 26.951452532380305 32.10914665473828 20.03489248476815 24-25 20.440976950708105 27.452472028952695 31.70090747505331 20.405643545285894 26-27 19.877005581771666 27.64544872496204 31.80622081489276 20.671324878373532 28-29 20.279692561082992 27.395861927768372 32.22008091290791 20.104364598240725 30-31 20.620532173559944 27.92522908401453 31.459869307254674 19.994369435170853 32-33 20.013772318414425 27.43099161678232 32.149971409168245 20.405264655635015 34-35 20.635192195126347 27.466054823419185 31.69422509923162 20.204527882222852 36-37 20.87791421996285 27.357086776929325 31.177471870180984 20.58752713292684 38-39 20.432799482583118 27.708104956440632 31.604789978208874 20.254305582767376 40-41 20.29223653511442 27.547285825407275 31.61821507391907 20.542262565559234 42-43 20.229275233415798 27.451688669300978 31.55533490695646 20.76370119032676 44-45 20.442784461677082 27.71346298855048 31.023839364706003 20.819913185066437 46-47 20.981774675045592 28.042205793370282 30.154058326531004 20.82196120505312 48-49 20.95057595549236 27.874523838706416 29.94100524461186 21.233894961189364 50-51 21.01473048091599 28.067916218453792 29.428310345410207 21.489042955220015 52-53 20.899344700530776 28.351864317908316 29.449037903345825 21.299753078215083 54-55 21.033212098581924 28.944791618927095 29.292092820440146 20.72990346205083 56-57 21.211871615522114 28.295268219993343 29.166329631258975 21.326530533225565 58-59 21.12802378655181 27.906050582678294 29.49770374732526 21.46822188344463 60-61 21.27303830003416 28.253706220999277 29.30261822833019 21.170637250636375 62-63 20.81622276879658 28.170153383827905 29.580565388363674 21.43305845901184 64-65 20.844461456115674 28.427441283745083 29.26709726344803 21.460999996691218 66-67 20.809473803376637 28.19784584138742 29.351143286157782 21.64153706907816 68-69 21.212587800253864 28.705824240449086 28.915516243702605 21.166071715594448 70-71 21.378481167540272 28.23475724844446 28.819345443137944 21.567416140877327 72-73 21.09125340776892 28.228776793555642 28.97084234591781 21.70912745275763 74-75 21.008371178006037 28.549929095579497 28.94522396792584 21.496475758488625 76-77 21.0583691839781 28.706927570234093 28.764240260485863 21.470462985301946 78-79 21.13386120125704 28.585539856313915 29.0018604750051 21.278738467423945 80-81 21.02878467070347 28.611389345625277 28.908461204659545 21.45136477901171 82-83 20.875183178136446 28.55883721165987 29.185613377832997 21.380366232370687 84-85 20.705920070376727 28.670469258898606 28.888573187345933 21.735037483378736 86-87 20.96096683417524 28.641495111710753 28.839811802958437 21.557726251155568 88-89 20.808873395042603 28.675682356157257 28.994373483783033 21.521070765017107 90-91 20.598339324592587 28.44551145538813 29.09924417346459 21.856905046554694 92-93 20.721237106434746 28.727201304351503 28.843922741390916 21.707638847822842 94-95 20.69578063102604 28.28044253218317 29.130118628986857 21.893658207803934 96-97 20.67667672318663 28.75649388248902 28.823865878336875 21.742963515987473 98-99 20.24031071984449 28.61321254883861 28.919005852502412 22.227470878814486 100-101 20.257376814671346 28.863510413680583 28.72135400093602 22.157758770712046 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4610.0 1 3050.5 2 2050.0 3 2582.0 4 1997.0 5 1643.0 6 3049.0 7 6676.0 8 10978.0 9 12559.5 10 10288.5 11 7904.5 12 7885.0 13 8745.5 14 9467.0 15 9870.5 16 10308.0 17 11052.5 18 11986.0 19 13075.5 20 14536.5 21 16464.0 22 20497.5 23 28326.0 24 35183.5 25 41416.0 26 53757.5 27 70978.0 28 96680.5 29 116762.0 30 122384.0 31 134314.5 32 155837.5 33 174878.0 34 194285.0 35 220033.0 36 237862.5 37 247567.5 38 259063.0 39 265939.0 40 270855.5 41 269206.0 42 266815.5 43 268057.0 44 264419.0 45 257616.5 46 251957.0 47 248600.0 48 239545.0 49 234023.5 50 233025.0 51 217101.0 52 188272.5 53 164593.0 54 150787.0 55 143789.0 56 129317.0 57 110051.5 58 96793.0 59 84917.0 60 72215.5 61 58919.0 62 48282.5 63 39265.5 64 31437.0 65 26085.0 66 21282.5 67 16247.0 68 11913.0 69 8769.5 70 6449.0 71 4599.5 72 3308.5 73 2472.5 74 1975.0 75 1561.0 76 1092.5 77 766.0 78 562.5 79 381.5 80 246.0 81 173.5 82 132.5 83 93.0 84 63.0 85 40.0 86 24.5 87 12.0 88 8.0 89 6.5 90 8.0 91 7.0 92 4.5 93 3.0 94 2.5 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05027885687477834 2 0.002013407038110698 3 1.4079769497277606E-4 4 2.252763119564417E-4 5 0.002323161967050805 6 0.0016895723396733128 7 0.0 8 0.0 9 1.1263815597822085E-4 10-11 3.5199423743194016E-5 12-13 0.0 14-15 4.927919324047162E-4 16-17 0.0 18-19 4.927919324047162E-5 20-21 4.64632393410161E-4 22-23 0.0 24-25 6.335896273774922E-4 26-27 0.00734963967757891 28-29 0.00782131195573771 30-31 0.0011545410987767637 32-33 0.006504853507742255 34-35 0.008025468613448236 36-37 0.004463286930637002 38-39 0.002611797241744996 40-41 0.0018092503804001724 42-43 0.0013446179869900114 44-45 5.561508951424654E-4 46-47 6.547092816234087E-4 48-49 0.002076766000848447 50-51 0.001534694875203259 52-53 0.0019711677296188647 54-55 7.391878986070744E-4 56-57 2.7455550519691333E-4 58-59 7.955069765961849E-4 60-61 0.005786785263381096 62-63 0.0024428400077776647 64-65 0.0010630225970444593 66-67 0.00570230664639743 68-69 0.0076664344912676565 70-71 0.0029567515944282975 72-73 7.251081291097967E-4 74-75 0.0031327487131442675 76-77 0.0017247717634165068 78-79 0.002400600699285832 80-81 9.433445563175996E-4 82-83 1.8303700346460888E-4 84-85 5.772705493883819E-4 86-87 0.005132075981757687 88-89 0.0030201105571660463 90-91 0.0013798174107332055 92-93 1.2671792547549847E-4 94-95 4.99831817153355E-4 96-97 4.435127391642446E-4 98-99 5.983902036342983E-4 100-101 1.4079769497277606E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7102389.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.56072200977018 #Duplication Level Percentage of deduplicated Percentage of total 1 68.51471754947505 29.845505647506048 2 17.735701482692974 15.451599238717147 3 6.373656060992648 8.329231795763626 4 2.8143039140472323 4.903724418032786 5 1.4559439566098307 3.171098497784287 6 0.8134025942045613 2.1259442572902474 7 0.48811535968388864 1.4883860244322202 8 0.32381763138918673 1.1284583858245276 9 0.22879231022517807 0.8969722401322874 >10 1.0076193320956268 7.97587755346966 >50 0.10942769247073081 3.369437221942749 >100 0.11246420094616019 10.247468767195278 >500 0.014517644813280859 4.376214305465252 >1k 0.007389483043312643 5.356013470070472 >5k 3.269682760242017E-5 0.10826084672624871 >10k+ 9.809048280726048E-5 1.2258073296471343 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36148 0.5089555077875909 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29822 0.4198868859478128 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20094 0.2829188882782962 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7601 0.10702032794880707 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.4079769497277606E-5 0.0 5 0.0 0.0 0.0 1.4079769497277606E-5 0.0 6 0.0 0.0 0.0 1.4079769497277606E-5 0.0 7 0.0 0.0 0.0 2.815953899455521E-5 0.0 8 0.0 0.0 0.0 2.815953899455521E-5 0.0 9 0.0 0.0 0.0 2.815953899455521E-5 0.0 10-11 1.4079769497277606E-5 0.0 0.0 3.5199423743194016E-5 0.0 12-13 1.4079769497277606E-5 0.0 0.0 6.335896273774923E-5 0.0 14-15 1.4079769497277606E-5 0.0 0.0 1.1967804072685964E-4 0.0 16-17 3.5199423743194016E-5 0.0 0.0 1.4079769497277606E-4 0.0 18-19 4.223930849183282E-5 0.0 0.0 1.4783757972141486E-4 0.0 20-21 4.223930849183282E-5 0.0 0.0 1.971167729618865E-4 0.0 22-23 4.223930849183282E-5 0.0 0.0 2.8863527469419093E-4 0.0 24-25 4.223930849183282E-5 0.0 0.0 4.083133154210506E-4 0.0 26-27 4.223930849183282E-5 0.0 0.0 7.321480138584355E-4 0.0 28-29 4.223930849183282E-5 0.0 0.0 0.0015276549904546203 0.0 30-31 4.223930849183282E-5 0.0 0.0 0.0036607400692921775 0.0 32-33 4.223930849183282E-5 0.0 0.0 0.007624195182775824 0.0 34-35 4.223930849183282E-5 0.0 0.0 0.013995290880293942 0.0 36-37 4.223930849183282E-5 0.0 0.0 0.024386160769284813 0.0 38-39 4.223930849183282E-5 0.0 0.0 0.04140860209149344 0.0 40-41 4.223930849183282E-5 0.0 0.0 0.05864927984090987 0.0 42-43 4.223930849183282E-5 0.0 0.0 0.07620675240401503 0.0 44-45 4.9279193240471625E-5 0.0 0.0 0.09815007316552218 0.0 46-47 5.631907798911042E-5 0.0 0.0 0.12510579186806017 0.0 48-49 7.743873223502683E-5 0.0 0.0 0.15654591715548105 0.0 50-51 8.447861698366564E-5 0.0 0.0 0.19023176567771774 0.0 52-53 8.447861698366564E-5 0.0 0.0 0.22240403897899708 0.0 54-55 8.447861698366564E-5 0.0 0.0 0.25877912347521376 0.0 56-57 8.447861698366564E-5 0.0 0.0 0.30270096442197125 0.0 58-59 8.447861698366564E-5 0.0 0.0 0.3553733821112868 0.0 60-61 8.447861698366564E-5 0.0 0.0 0.4127062035042012 0.0 62-63 8.447861698366564E-5 0.0 0.0 0.4699123069716401 0.0 64-65 8.447861698366564E-5 0.0 0.0 0.5304834753489284 0.0 66-67 8.447861698366564E-5 1.4079769497277606E-5 0.0 0.6020579835883391 0.0 68-69 9.855838648094324E-5 1.4079769497277606E-5 0.0 0.687310987894355 0.0 70-71 9.855838648094324E-5 1.4079769497277606E-5 0.0 0.783398374828526 0.0 72-73 9.855838648094324E-5 1.4079769497277606E-5 0.0 0.8858568574602151 0.0 74-75 9.855838648094324E-5 1.4079769497277606E-5 0.0 0.9895050806144243 0.0 76-77 9.855838648094324E-5 1.4079769497277606E-5 0.0 1.100685980449677 0.0 78-79 9.855838648094324E-5 1.4079769497277606E-5 0.0 1.2344719502128088 0.0 80-81 9.855838648094324E-5 1.4079769497277606E-5 0.0 1.38791327819414 0.0 82-83 9.855838648094324E-5 1.4079769497277606E-5 0.0 1.5522453078816156 0.0 84-85 9.855838648094324E-5 1.4079769497277606E-5 0.0 1.7171546081184794 0.0 86-87 9.855838648094324E-5 1.4079769497277606E-5 0.0 1.8970940622936874 0.0 88-89 9.855838648094324E-5 1.4079769497277606E-5 0.0 2.104763622493784 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16255 0.0 62.79513 1 GTATCAA 32895 0.0 44.59492 1 TGGTATC 5860 0.0 37.124184 2 GTGGTAT 6235 0.0 34.830395 1 TATAACG 710 0.0 34.119358 2 ATCAACG 45065 0.0 32.452423 3 TCAACGC 45710 0.0 32.04645 4 TATCAAC 46505 0.0 31.50972 2 CAACGCA 47035 0.0 31.163885 5 AACGCAG 50975 0.0 28.922049 6 CGCAGAG 56695 0.0 25.610334 8 ACGCAGA 58795 0.0 24.897562 7 GCAGAGT 60050 0.0 23.760443 9 GTACATG 36930 0.0 23.213247 1 TACATGG 35595 0.0 22.698877 2 AGTACTT 38370 0.0 21.588491 12-13 ACATGGG 36595 0.0 21.545826 3 AGAGTAC 58170 0.0 21.441902 10-11 GAGTACT 38555 0.0 20.80734 12-13 GTACTTT 38625 0.0 20.456202 14-15 >>END_MODULE