FastQCFastQC Report
Wed 25 May 2016
SRR2049402_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049402_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9688190
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357270.3687685728706807No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298040.3076322821909975No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157420.1624864912847498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA202700.057.79131
GTATCAA383150.042.5997921
ATCAACG468000.034.4601483
TCAACGC473200.034.0730064
TATCAAC484600.033.3851282
GTGGTAT77600.033.117591
CAACGCA495200.032.6353345
AACGCAG525400.031.0037386
TGGTATC82000.030.4114592
CGCAGAG578300.027.971828
ACGCAGA589050.027.5419927
GCAGAGT612900.025.6563689
GTACATG392100.024.8480321
TACATGG382800.024.1717912
ACATGGG394350.022.827093
GAGTACT393800.022.71468512-13
CAGAGTA597850.022.26430710-11
GTACTTT412650.021.37560814-15
AGAGTAC573750.021.16685510-11
TATAACG9750.020.4614522
Produced by FastQC (version 0.11.5)