##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049402_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9688190 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.447883350760048 31.0 31.0 34.0 30.0 34.0 2 31.488188402580874 33.0 31.0 34.0 30.0 34.0 3 31.509512406342154 33.0 31.0 34.0 28.0 34.0 4 35.12823561470202 37.0 35.0 37.0 32.0 37.0 5 35.153031577621825 37.0 35.0 37.0 33.0 37.0 6 35.240654549508214 37.0 35.0 37.0 33.0 37.0 7 35.186065198969054 37.0 35.0 37.0 32.0 37.0 8 35.15741103343349 37.0 35.0 37.0 32.0 37.0 9 36.73396093594366 39.0 37.0 39.0 33.0 39.0 10-11 36.66844069944953 39.0 37.0 39.0 32.5 39.0 12-13 36.628207229627 39.0 37.0 39.0 32.0 39.0 14-15 37.78963986048994 40.0 38.0 41.0 33.0 41.0 16-17 37.78063786940595 40.0 38.0 41.0 33.0 41.0 18-19 37.81736258269088 40.0 38.0 41.0 33.0 41.0 20-21 37.78465874430621 40.0 38.0 41.0 32.5 41.0 22-23 37.68037084326381 40.0 38.0 41.0 32.0 41.0 24-25 37.57076409525412 40.0 37.5 41.0 32.0 41.0 26-27 37.42809214105009 40.0 37.0 41.0 32.0 41.0 28-29 37.27315742156172 40.0 37.0 41.0 32.0 41.0 30-31 37.080593227424316 40.0 37.0 41.0 31.0 41.0 32-33 36.82859249250892 40.0 36.0 41.0 31.0 41.0 34-35 36.62629572706564 39.0 36.0 41.0 30.0 41.0 36-37 36.43539804648753 39.0 36.0 41.0 30.0 41.0 38-39 36.227781814766224 39.0 35.0 41.0 30.0 41.0 40-41 36.009034814552564 39.0 35.0 40.0 29.5 41.0 42-43 35.77091040741357 39.0 35.0 40.0 28.5 41.0 44-45 35.47282691606998 39.0 35.0 40.0 27.5 41.0 46-47 35.098184335773766 38.0 35.0 40.0 25.5 41.0 48-49 34.79033653344949 38.0 34.0 40.0 24.0 41.0 50-51 33.41994144417069 36.5 32.5 39.0 23.0 40.0 52-53 33.709421006400575 37.5 33.5 39.0 23.5 40.0 54-55 34.76667602513989 38.5 34.5 40.0 24.5 41.0 56-57 34.886419909188405 39.0 35.0 40.0 25.0 41.0 58-59 34.73422558806134 38.5 34.5 40.0 25.0 41.0 60-61 34.48491586147671 38.0 34.0 40.0 24.0 41.0 62-63 34.22385363003822 38.0 34.0 40.0 23.0 41.0 64-65 33.952883201093286 37.5 34.0 40.0 23.0 41.0 66-67 33.638411457661334 37.0 33.5 40.0 22.0 41.0 68-69 33.29409781393635 36.5 33.0 39.5 21.5 41.0 70-71 32.96254537741312 36.0 33.0 39.0 20.0 41.0 72-73 32.706334774607015 36.0 33.0 39.0 20.0 41.0 74-75 32.50569796835116 35.0 33.0 39.0 19.5 40.0 76-77 32.161172984840306 35.0 32.5 37.5 18.0 40.0 78-79 31.789895893866657 35.0 32.0 37.0 17.0 39.0 80-81 31.447919993311444 35.0 32.0 37.0 13.5 39.0 82-83 31.098000452096834 35.0 32.0 36.0 9.0 38.5 84-85 30.773430434374223 35.0 31.5 36.0 7.0 37.0 86-87 30.472369400269812 35.0 31.0 35.5 3.5 37.0 88-89 30.1769559639107 35.0 31.0 35.0 2.0 36.5 90-91 29.906722979214898 34.5 31.0 35.0 2.0 36.0 92-93 29.65017830988038 34.0 31.0 35.0 2.0 36.0 94-95 29.38728302190605 34.0 31.0 35.0 2.0 36.0 96-97 29.129320595487908 34.0 30.0 35.0 2.0 35.0 98-99 28.812356797296502 34.0 30.0 35.0 2.0 35.0 100-101 28.270581192152505 34.0 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2978767543214218 1101 2 0.30151950028277597 1101 3 0.31689174345821414 1101 4 0.33165596043470913 1101 5 0.2675308787913835 1101 6 0.30289254004276245 1101 7 0.3044408349383616 1101 8 0.3009381660242525 1101 9 0.35868538170726083 1101 10-11 0.3884892692746078 1101 12-13 0.371712891155461 1101 14-15 0.4470483759055526 1101 16-17 0.44268424456375044 1101 18-19 0.36680111017697925 1101 20-21 0.3129899012800337 1101 22-23 0.31217295593865657 1101 24-25 0.30766643517057446 1101 26-27 0.31595253845227944 1101 28-29 0.30563979661547336 1101 30-31 0.29620667758536 1101 32-33 0.2307192917063503 1101 34-35 0.2615475755210426 1101 36-37 0.23324434357329693 1101 38-39 0.13541386820996593 1101 40-41 0.10437375263810367 1101 42-43 0.08397853503566921 1101 44-45 -0.08048414558766126 1101 46-47 -0.013817924362811596 1101 48-49 -0.1912467515799392 1101 50-51 -0.24938771512279345 1101 52-53 -0.09109840514383194 1101 54-55 0.11544463755442536 1101 56-57 0.10223627483676268 1101 58-59 0.07490681904756968 1101 60-61 0.15063867252589347 1101 62-63 0.09721950560216186 1101 64-65 0.09830305129521122 1101 66-67 0.06701417462556236 1101 68-69 0.03273953999280366 1101 70-71 -0.03759208392814628 1101 72-73 -0.08586103625498964 1101 74-75 -0.12857410288602011 1101 76-77 -0.14077953331648274 1101 78-79 -0.28188882653212133 1101 80-81 -0.3167932171057153 1101 82-83 -0.3901319051462657 1101 84-85 -0.5068761818776295 1101 86-87 -0.5195281591800871 1101 88-89 -0.4947763598029695 1101 90-91 -0.5922924703208459 1101 92-93 -0.665775122470091 1101 94-95 -0.7784024989653915 1101 96-97 -0.7827076750655664 1101 98-99 -0.8836248652568344 1101 100-101 -1.0272292875412639 1102 1 0.2536364027047604 1102 2 0.24216093580043463 1102 3 0.24993600058654408 1102 4 0.26916168269227114 1102 5 0.26758683472508693 1102 6 0.2775274121967115 1102 7 0.2524882837806217 1102 8 0.28092497773747027 1102 9 0.3693969669298056 1102 10-11 0.34135722729640605 1102 12-13 0.2884629380911221 1102 14-15 0.40861276736122676 1102 16-17 0.31744782990502785 1102 18-19 0.35587468794614097 1102 20-21 0.30191319883669365 1102 22-23 0.2424130803762452 1102 24-25 0.25777843763223274 1102 26-27 0.3492489529486065 1102 28-29 0.33630458777972194 1102 30-31 0.24689663784712934 1102 32-33 0.22623726951013623 1102 34-35 0.2504699839118487 1102 36-37 0.2086942788274868 1102 38-39 0.16206006594332223 1102 40-41 0.16487088000609162 1102 42-43 0.046771748098748844 1102 44-45 0.06773029419278487 1102 46-47 -0.03290168828169726 1102 48-49 -0.018759320078984842 1102 50-51 -0.14811707699631427 1102 52-53 0.055714138854490614 1102 54-55 0.17427290128911466 1102 56-57 0.16248787499415585 1102 58-59 0.20678011298157628 1102 60-61 0.17825008495051264 1102 62-63 0.14387441783187427 1102 64-65 0.12347407255933973 1102 66-67 0.14257746789989767 1102 68-69 0.07711655472237311 1102 70-71 0.057551513671761256 1102 72-73 0.09604978606996184 1102 74-75 0.09619797482792336 1102 76-77 0.06553019556040596 1102 78-79 0.019716949945543405 1102 80-81 -0.010426997677164707 1102 82-83 -0.06913644696067323 1102 84-85 -0.029381092477766657 1102 86-87 -0.033108260529782996 1102 88-89 -0.14114262083776907 1102 90-91 -0.17489180004414706 1102 92-93 -0.29822140270460906 1102 94-95 -0.3950734056669276 1102 96-97 -0.45719832451923637 1102 98-99 -0.5685002895427331 1102 100-101 -0.5563396322622509 1103 1 0.23544519378273066 1103 2 0.2045981688391123 1103 3 0.22978135706568992 1103 4 0.23121171831110843 1103 5 0.2682222965917589 1103 6 0.2437531146282339 1103 7 0.28149590823535675 1103 8 0.22941981934567224 1103 9 0.33631979746354546 1103 10-11 0.32105191043847725 1103 12-13 0.32473062346736725 1103 14-15 0.3872958704522702 1103 16-17 0.37763637908543757 1103 18-19 0.3922137858728689 1103 20-21 0.34107640881388335 1103 22-23 0.29908268202729715 1103 24-25 0.34314494929837025 1103 26-27 0.38643512720584283 1103 28-29 0.35530157382246585 1103 30-31 0.34348425038321295 1103 32-33 0.3210137949326466 1103 34-35 0.29456118770905704 1103 36-37 0.2878104435344042 1103 38-39 0.300158662693633 1103 40-41 0.2172145787053097 1103 42-43 0.17870617919984255 1103 44-45 0.16113382615921523 1103 46-47 0.18916581030225643 1103 48-49 0.19696181328222906 1103 50-51 0.08140523286241574 1103 52-53 0.28923091830862546 1103 54-55 0.39798882269645475 1103 56-57 0.4556508009419744 1103 58-59 0.41591048214276327 1103 60-61 0.43418705162901716 1103 62-63 0.34698680007287663 1103 64-65 0.3073508120019781 1103 66-67 0.31716739809687766 1103 68-69 0.3100712362559932 1103 70-71 0.24986264368404676 1103 72-73 0.2837559049024563 1103 74-75 0.16877123251364878 1103 76-77 0.12340459209129762 1103 78-79 0.17943492592725718 1103 80-81 0.15349202925586525 1103 82-83 0.17605394388937512 1103 84-85 0.2010497413480543 1103 86-87 0.09429883133682537 1103 88-89 0.08007267766914339 1103 90-91 0.10913769315792621 1103 92-93 0.019474186908738034 1103 94-95 -0.009064982931416665 1103 96-97 -0.03852065398575277 1103 98-99 -0.016583253374498952 1103 100-101 -0.025648406545425217 1104 1 0.0033230708843916545 1104 2 0.02384840277878908 1104 3 0.0991180428727283 1104 4 0.028273698972370198 1104 5 0.017646338077817347 1104 6 -0.03916818346537809 1104 7 -0.04759724523813702 1104 8 -0.03455690278388346 1104 9 -0.04340389749607709 1104 10-11 -0.00436644734968894 1104 12-13 -0.06486244684587206 1104 14-15 -0.004732609680580424 1104 16-17 -0.024287874912026552 1104 18-19 -0.05493538437613665 1104 20-21 -0.03906468166729837 1104 22-23 -0.03656490340453189 1104 24-25 -0.020613846271828606 1104 26-27 -0.07360773330616865 1104 28-29 -0.10860277877153379 1104 30-31 -0.05843316823897737 1104 32-33 -0.08720550103578972 1104 34-35 -0.13905279856070507 1104 36-37 -0.2223718586329042 1104 38-39 -0.23084602118474606 1104 40-41 -0.2573124211415845 1104 42-43 -0.23154900619814356 1104 44-45 -0.19940633082643444 1104 46-47 -0.22123963352396459 1104 48-49 -0.28551543653621536 1104 50-51 -0.3806561368871826 1104 52-53 -0.2936675073273065 1104 54-55 -0.1539227629332629 1104 56-57 -0.24528607893136467 1104 58-59 -0.22301846790337265 1104 60-61 -0.222384647987667 1104 62-63 -0.2837172815068314 1104 64-65 -0.27956823137642317 1104 66-67 -0.19293194699707072 1104 68-69 -0.227037073036648 1104 70-71 -0.2068486382549537 1104 72-73 -0.17358783159416902 1104 74-75 -0.14360966810581033 1104 76-77 -0.18231341187659922 1104 78-79 -0.1817996416240817 1104 80-81 -0.25586945166652697 1104 82-83 -0.2780618179003831 1104 84-85 -0.2057732208280818 1104 86-87 -0.22377547840332923 1104 88-89 -0.27537544776149403 1104 90-91 -0.2942928389337318 1104 92-93 -0.24342581774056882 1104 94-95 -0.29658585296798634 1104 96-97 -0.35133504868587195 1104 98-99 -0.3869374358457982 1104 100-101 -0.44792069221842823 1105 1 0.1853217168278718 1105 2 0.19886559690337435 1105 3 0.1889381908747687 1105 4 0.20211626689167161 1105 5 0.2080841304255756 1105 6 0.21130657845290557 1105 7 0.24684913140669096 1105 8 0.23801827691997346 1105 9 0.2855056190149341 1105 10-11 0.2560066308331983 1105 12-13 0.21168222646699064 1105 14-15 0.2764061825060864 1105 16-17 0.27900920886690983 1105 18-19 0.27167839942092087 1105 20-21 0.3062752177869825 1105 22-23 0.24555933146563547 1105 24-25 0.3148668112139461 1105 26-27 0.35149224013478886 1105 28-29 0.2895612968820487 1105 30-31 0.26933385027666645 1105 32-33 0.2828025732259434 1105 34-35 0.36147091379985596 1105 36-37 0.3486188020161123 1105 38-39 0.30320513790803716 1105 40-41 0.3027059230827618 1105 42-43 0.28415327733485896 1105 44-45 0.25450394893447026 1105 46-47 0.21445437125780842 1105 48-49 0.20709680658205087 1105 50-51 0.16159715134699582 1105 52-53 0.2669144329059421 1105 54-55 0.4036384084202851 1105 56-57 0.4159543215877477 1105 58-59 0.46469104931249205 1105 60-61 0.41550834835353356 1105 62-63 0.43022884292744834 1105 64-65 0.420979825069729 1105 66-67 0.395275602550349 1105 68-69 0.40429867450217216 1105 70-71 0.40309219051599143 1105 72-73 0.46574559656657044 1105 74-75 0.4070484254103093 1105 76-77 0.3892569088862885 1105 78-79 0.42676765494810454 1105 80-81 0.4135954529973134 1105 82-83 0.4119925259012156 1105 84-85 0.4052993077452278 1105 86-87 0.32339146915978745 1105 88-89 0.3249725798430312 1105 90-91 0.33418794423728215 1105 92-93 0.3516174490000843 1105 94-95 0.35019682235378724 1105 96-97 0.32396531626846325 1105 98-99 0.3088492629195727 1105 100-101 0.4205227197794308 1106 1 0.26854111140879766 1106 2 0.2879044438922058 1106 3 0.26658561291861105 1106 4 0.21339007764063211 1106 5 0.26276983098963314 1106 6 0.2961725717782997 1106 7 0.35533352547305697 1106 8 0.3567057024922349 1106 9 0.3514259255959402 1106 10-11 0.3844143796484829 1106 12-13 0.40701980371256496 1106 14-15 0.4101678275366467 1106 16-17 0.48142814083200136 1106 18-19 0.4616424780852171 1106 20-21 0.4475715222509322 1106 22-23 0.4687837246498887 1106 24-25 0.4691656100789743 1106 26-27 0.5276587034269014 1106 28-29 0.4767259126511689 1106 30-31 0.48892200456688073 1106 32-33 0.4941173039139457 1106 34-35 0.4608720615183799 1106 36-37 0.45257504953519145 1106 38-39 0.46126391560250823 1106 40-41 0.49996820764051364 1106 42-43 0.4755238257600567 1106 44-45 0.5120190060018501 1106 46-47 0.5362406858659767 1106 48-49 0.4868960783541496 1106 50-51 0.5028216621454433 1106 52-53 0.566954372726677 1106 54-55 0.5806293962325171 1106 56-57 0.6848399760862449 1106 58-59 0.6917109506145778 1106 60-61 0.6858482533800654 1106 62-63 0.6523653047984013 1106 64-65 0.6795117548736513 1106 66-67 0.625435557856953 1106 68-69 0.6231762430246945 1106 70-71 0.6688475921929751 1106 72-73 0.6222802903040972 1106 74-75 0.6276902068733321 1106 76-77 0.6214787880671011 1106 78-79 0.6172548717551756 1106 80-81 0.553076088309993 1106 82-83 0.5991926709587254 1106 84-85 0.6384216706144237 1106 86-87 0.6149656371618626 1106 88-89 0.6455937788217483 1106 90-91 0.595928536326749 1106 92-93 0.5894590814851277 1106 94-95 0.6352728060250179 1106 96-97 0.6030221484281064 1106 98-99 0.6342485386121481 1106 100-101 0.6644153576208218 1107 1 0.27431112303609595 1107 2 0.2527660258775377 1107 3 0.23407485908547798 1107 4 0.25261883279075903 1107 5 0.24819809644301927 1107 6 0.22763546352082642 1107 7 0.2650939917385102 1107 8 0.2865713768945639 1107 9 0.3561804633163774 1107 10-11 0.2798353836829861 1107 12-13 0.3061783823443136 1107 14-15 0.4303909866560005 1107 16-17 0.3502785468898111 1107 18-19 0.3134142280469874 1107 20-21 0.3501595540946383 1107 22-23 0.436921082817058 1107 24-25 0.4091365108926084 1107 26-27 0.44882470409078223 1107 28-29 0.44345955063878506 1107 30-31 0.4043821005808468 1107 32-33 0.4097932100927508 1107 34-35 0.4528710069310904 1107 36-37 0.40701945719515464 1107 38-39 0.4607721713181405 1107 40-41 0.4607642037708146 1107 42-43 0.5362724486564687 1107 44-45 0.5536092998490716 1107 46-47 0.516777496562888 1107 48-49 0.4573376328169445 1107 50-51 0.46711699864377465 1107 52-53 0.5188095430349264 1107 54-55 0.553088692441392 1107 56-57 0.5637170183180302 1107 58-59 0.5655103759507227 1107 60-61 0.6096924960510108 1107 62-63 0.6231758517334427 1107 64-65 0.6252974700096345 1107 66-67 0.6786545060479838 1107 68-69 0.6609237983599527 1107 70-71 0.638638752873149 1107 72-73 0.6244445709382447 1107 74-75 0.6319782463125421 1107 76-77 0.608417406638317 1107 78-79 0.5584382420326826 1107 80-81 0.5289655747667155 1107 82-83 0.5178957702986118 1107 84-85 0.5670686157960496 1107 86-87 0.6006117673938256 1107 88-89 0.6465714973081873 1107 90-91 0.6636015002986859 1107 92-93 0.6420042880102024 1107 94-95 0.6678627407338347 1107 96-97 0.7199259089376433 1107 98-99 0.8078581640827203 1107 100-101 0.8541291212540791 1108 1 0.24358099484630813 1108 2 0.2506760028822157 1108 3 0.2187624415945031 1108 4 0.20889624523214678 1108 5 0.23072204507194272 1108 6 0.2836469512795432 1108 7 0.29752490976270707 1108 8 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-1.0895533070618981 2316 96-97 -0.8152742473865473 2316 98-99 -0.6451328887583045 2316 100-101 -0.6132833915498388 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 90131.0 3 19375.0 4 15239.0 5 13624.0 6 14049.0 7 15294.0 8 17451.0 9 20786.0 10 25063.0 11 29556.0 12 36047.0 13 42528.0 14 48279.0 15 56521.0 16 70350.0 17 89854.0 18 72756.0 19 45117.0 20 40870.0 21 41676.0 22 43672.0 23 47369.0 24 54423.0 25 64762.0 26 78001.0 27 94659.0 28 116005.0 29 141582.0 30 175883.0 31 223788.0 32 290187.0 33 389967.0 34 548996.0 35 818031.0 36 1281528.0 37 1929881.0 38 2065027.0 39 519679.0 40 184.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.47372153232674 20.564807556892504 15.324826233984151 21.636644676796603 2 15.252006967976458 20.642196721054262 40.87815868042811 23.227637630541174 3 17.834046379464272 26.48758812520393 31.337430385230714 24.340935110101086 4 12.272272425198414 17.673621082373458 39.594564659302314 30.459541833125815 5 12.617553273407331 37.998294375651064 35.34430614202306 14.039846208918544 6 28.758049841351568 38.91111579528913 18.28857477369192 14.042259589667383 7 25.92977945984533 33.944600151299376 22.761224957233512 17.364395431621777 8 25.330237072082213 35.459313675852336 20.552595307114206 18.65785394495125 9 25.32216695719175 17.01554796100116 22.112719878419227 35.549565203387864 10-11 23.589854413008894 27.35531624365 28.82560178144733 20.229227561893776 12-13 24.6617115161365 25.053503073200833 29.086130760561208 21.19865465010146 14-15 21.945604849300366 26.728027498003808 27.266512352791473 24.05985529990435 16-17 20.512074114148447 30.30097518018759 27.827334547056974 21.359616158606986 18-19 20.519463320089287 28.347696061680377 30.953967271136325 20.17887334709401 20-21 21.69677033347748 27.29424756986802 30.874648299401773 20.134333797252722 22-23 21.349043175952602 27.193666558750394 31.019786042963272 20.437504222333732 24-25 21.0224811408113 27.457121691180586 30.75934360624509 20.76105356176302 26-27 20.719450792601066 27.684114189714045 30.76116136850986 20.835273649175036 28-29 20.761092810350654 27.57506563191504 30.96975863846471 20.694082919269597 30-31 21.034375108218775 27.84798138794491 30.569812263554073 20.547831240282243 32-33 20.72379978967908 27.690690553684068 30.979772817074064 20.605736839562795 34-35 21.120011337716342 27.57039675194658 30.627141691933275 20.682450218403805 36-37 21.352429831403793 27.523098280057955 30.33849195179344 20.785979936744813 38-39 21.004242456584777 27.847886136474344 30.460618419513835 20.687252987427048 40-41 20.947807762954643 27.66212304273305 30.492261766826473 20.897807427485837 42-43 21.066332298626307 27.72979566579126 30.253106250020966 20.95076578556146 44-45 21.14728887343699 27.97663091029885 29.943766198856824 20.932314017407332 46-47 21.48221875748763 28.08318508189849 29.50780282753588 20.926793333078002 48-49 21.53924985082868 28.01238458738317 29.287658100827272 21.160707460960882 50-51 21.43313136620325 28.2757164869745 28.95204788166768 21.339104265154567 52-53 21.386140484319334 28.435276878963112 28.937954121636466 21.24062851508109 54-55 21.442080538093975 28.704251619775157 28.892832049454864 20.960835792676004 56-57 21.37632606732601 28.43568751530296 28.818834419181172 21.369151998189864 58-59 21.406815538892314 28.167603601931535 29.08256902988994 21.343011829286212 60-61 21.49990374744982 28.36213254363234 28.954026375779247 21.18393733313859 62-63 21.267369556047562 28.348192063275217 29.114214767634085 21.270223613043132 64-65 21.414845650672838 28.221757992678658 29.048705789976452 21.314690566672056 66-67 21.22097978777601 28.298300631884572 29.008055337342896 21.472664242996526 68-69 21.510121937026685 28.5604267344344 28.696032743625675 21.23341858491323 70-71 21.56254035291843 28.253132934783697 28.6170227150997 21.567303997198174 72-73 21.351669310385493 28.40863609655496 28.822480667564164 21.417213925495385 74-75 21.38319113146288 28.615242505287313 28.65610729675998 21.345459066489823 76-77 21.510574092974146 28.636931900118125 28.48061549622742 21.371878510680315 78-79 21.486910246787154 28.57608034057291 28.72768200573963 21.209327406900304 80-81 21.447823788702696 28.66473241261932 28.604579248644967 21.282864550033015 82-83 21.331541880963048 28.577885852206215 28.87270123901333 21.21787102781741 84-85 21.280878356226378 28.57701906235006 28.639177328661408 21.502925252762154 86-87 21.432781136667785 28.610333072296285 28.549678474373767 21.40720731666216 88-89 21.2897075135566 28.62267805764344 28.69184163919082 21.395772789609143 90-91 21.201793467391795 28.560774674692112 28.663939047913967 21.57349281000213 92-93 21.27859062517644 28.615733306551327 28.638023545800586 21.467652522471646 94-95 21.26461598853063 28.46434171287172 28.79125947099748 21.479782827600168 96-97 21.31116126471728 28.621291358446495 28.579838078285384 21.48770929855084 98-99 21.165188097850056 28.535203976432705 28.61411947414098 21.68548845157626 100-101 21.25690363490359 28.57519277943353 28.465753501019446 21.702150084643435 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1739.0 1 1162.5 2 1003.0 3 1509.5 4 1353.0 5 1255.5 6 1953.5 7 4027.5 8 7426.0 9 9853.0 10 9748.0 11 9214.0 12 9763.5 13 10638.0 14 11435.5 15 12144.0 16 12972.5 17 13932.0 18 15087.0 19 16662.0 20 19024.5 21 22438.5 22 28925.0 23 40534.5 24 52720.5 25 65270.5 26 84915.5 27 111328.0 28 146734.5 29 179397.0 30 195554.0 31 215621.0 32 243726.5 33 267780.0 34 287250.5 35 305306.5 36 319546.0 37 326761.5 38 333451.0 39 338399.0 40 343175.5 41 341170.5 42 332696.0 43 326584.0 44 319342.5 45 310294.0 46 302323.5 47 293805.0 48 284177.5 49 279816.5 50 276092.0 51 261775.0 52 240497.0 53 225350.5 54 217206.5 55 209277.0 56 191136.0 57 169543.0 58 154011.5 59 139642.0 60 125301.0 61 109618.5 62 93830.0 63 79467.0 64 66155.5 65 54914.5 66 44724.0 67 35393.0 68 27747.0 69 21411.5 70 16660.0 71 12811.5 72 9876.0 73 7626.0 74 6059.5 75 4726.5 76 3601.5 77 2855.5 78 2182.0 79 1762.0 80 1365.5 81 990.0 82 779.5 83 617.0 84 523.0 85 438.5 86 369.0 87 326.5 88 280.5 89 220.0 90 187.5 91 156.5 92 124.0 93 106.0 94 95.0 95 81.0 96 62.5 97 56.0 98 44.5 99 22.5 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015038928840165191 2 0.004562255694820189 3 0.004242278485454972 4 0.006482118951011489 5 0.00249788660214137 6 0.007555590879204474 7 0.0011663685373635325 8 0.0038913357396995725 9 0.011684329064562111 10-11 0.008112970534227756 12-13 0.009248373535201106 14-15 0.0037107034440901757 16-17 0.006678234014815977 18-19 0.004557094772088491 20-21 8.721959416568007E-4 22-23 0.0012747479147291704 24-25 0.008293602829837153 26-27 0.001233460532875594 28-29 7.018854915107982E-4 30-31 6.244716505353425E-4 32-33 0.0014244146739483847 34-35 0.0017753574197037834 36-37 0.001352161755704626 38-39 0.0017650355742403897 40-41 0.002632070593165493 42-43 0.0027094844341409487 44-45 0.008030395770520603 46-47 0.014254468584947241 48-49 0.005197049190818925 50-51 0.005754428845842206 52-53 0.014017066139289177 54-55 0.008376177593544306 56-57 0.005388003331891716 58-59 0.006869188155888768 60-61 0.0013934491375582022 62-63 0.0023378979974587617 64-65 0.0022140358518980326 66-67 0.0027404499705311314 68-69 0.002038564479020333 70-71 0.002363702611117247 72-73 0.0013676445238997171 74-75 0.0014811848239970522 76-77 0.008123292379691149 78-79 0.006518245410133369 80-81 0.012917789597437706 82-83 0.01496667592192143 84-85 0.008484556970909943 86-87 0.005661532236671659 88-89 0.0029726914934574983 90-91 0.002606265979507008 92-93 9.599316280956506E-4 94-95 0.0013057134511193526 96-97 0.0018063229560939661 98-99 0.006322130346328881 100-101 0.0111785586368558 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9688190.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.47654353333796 #Duplication Level Percentage of deduplicated Percentage of total 1 67.11933930897115 29.852362167043367 2 16.99858291302813 15.120764258727007 3 6.5754964734989425 8.773660654706578 4 3.050457968715204 5.426953065687178 5 1.70408537216381 3.789591361978407 6 1.0425567287427513 2.7821591839140836 7 0.6681476141304364 2.0801827512597777 8 0.4542398235827137 1.6162413830521856 9 0.3660070065322813 1.4650853903585745 >10 1.806019845330389 14.532431561634498 >50 0.13340185823922404 4.064396757364657 >100 0.0738693401131495 6.041133382266454 >500 0.005167572703893586 1.551439350234659 >1k 0.0025351415991005893 1.983317158219902 >5k 2.3258162258459408E-5 0.07955898545696576 >10k+ 6.977448677537822E-5 0.8407225880956933 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35727 0.3687685728706807 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29804 0.3076322821909975 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15742 0.1624864912847498 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.0321845463394092E-5 0.0 4 0.0 0.0 0.0 1.0321845463394092E-5 0.0 5 0.0 0.0 0.0 1.0321845463394092E-5 0.0 6 0.0 0.0 0.0 1.0321845463394092E-5 0.0 7 0.0 0.0 0.0 1.0321845463394092E-5 0.0 8 0.0 0.0 0.0 1.0321845463394092E-5 0.0 9 2.0643690926788183E-5 0.0 0.0 1.0321845463394092E-5 0.0 10-11 2.0643690926788183E-5 1.0321845463394092E-5 0.0 2.5804613658485232E-5 0.0 12-13 2.0643690926788183E-5 2.0643690926788183E-5 0.0 3.612645912187932E-5 0.0 14-15 3.096553639018228E-5 3.096553639018228E-5 0.0 4.128738185357637E-5 0.0 16-17 3.096553639018228E-5 4.128738185357637E-5 0.0 4.644830458527341E-5 0.0 18-19 3.096553639018228E-5 4.128738185357637E-5 0.0 5.67701500486675E-5 0.0 20-21 3.096553639018228E-5 4.128738185357637E-5 0.0 7.74138409754557E-5 0.0 22-23 3.096553639018228E-5 4.644830458527341E-5 0.0 1.0837937736563795E-4 0.0 24-25 3.096553639018228E-5 5.160922731697046E-5 0.0 2.374024456580641E-4 0.0 26-27 3.096553639018228E-5 6.193107278036455E-5 0.0 3.767473594138843E-4 0.0 28-29 3.096553639018228E-5 7.74138409754557E-5 0.0 8.412304052666184E-4 0.0 30-31 3.096553639018228E-5 9.289660917054682E-5 0.0 0.0018992195652645127 0.0 32-33 3.096553639018228E-5 9.289660917054682E-5 0.0 0.004577738463015279 0.0 34-35 4.128738185357637E-5 9.289660917054682E-5 0.0 0.008892269866714009 0.0 36-37 4.128738185357637E-5 9.805753190224388E-5 0.0 0.015400193431383984 0.0 38-39 4.128738185357637E-5 1.13540300097335E-4 0.0 0.025618820440144133 0.0 40-41 4.128738185357637E-5 1.3418399102412318E-4 0.0 0.03828372482372869 0.0 42-43 4.128738185357637E-5 1.3418399102412318E-4 0.0 0.05009191603385152 0.0 44-45 4.128738185357637E-5 1.3934491375582024E-4 0.0 0.06490376427382205 0.0 46-47 4.128738185357637E-5 1.445058364875173E-4 0.0 0.08517586876392805 0.0 48-49 4.128738185357637E-5 1.445058364875173E-4 0.0 0.10842066474749154 0.0 50-51 4.128738185357637E-5 1.6514952741430547E-4 0.0 0.13270796712285782 0.0 52-53 4.128738185357637E-5 1.7031045014600252E-4 0.0 0.15691269473451697 0.0 54-55 4.128738185357637E-5 1.8579321834109365E-4 0.0 0.18390432062129253 0.0 56-57 5.1609227316970463E-5 2.7352890477994345E-4 0.0 0.21814704294610243 0.0 58-59 6.193107278036455E-5 2.890116729750346E-4 0.0 0.25670945759734276 0.0 60-61 6.193107278036455E-5 2.890116729750346E-4 0.0 0.29950382888857463 0.0 62-63 6.193107278036455E-5 2.993335184384286E-4 0.0 0.3439393736084862 0.0 64-65 6.193107278036455E-5 3.096553639018227E-4 0.0 0.39250881743648713 0.0 66-67 6.193107278036455E-5 3.251381320969139E-4 0.0 0.45101819844573654 0.0 68-69 6.193107278036455E-5 3.3029905482861093E-4 0.0 0.5176508718346771 0.0 70-71 6.193107278036455E-5 3.3545997756030796E-4 0.0 0.5911888598386283 0.0 72-73 6.193107278036455E-5 3.40620900292005E-4 0.0 0.6712347714072495 0.0 74-75 6.193107278036455E-5 3.40620900292005E-4 0.0 0.7557087546796667 0.0 76-77 6.193107278036455E-5 3.715864366821873E-4 0.0 0.8498852726876743 0.0 78-79 6.193107278036455E-5 3.715864366821873E-4 0.0 0.9604219157551617 0.0 80-81 6.193107278036455E-5 3.715864366821873E-4 0.0 1.0874528678731528 0.0 82-83 6.193107278036455E-5 3.715864366821873E-4 0.0 1.2214717093698617 0.0 84-85 6.193107278036455E-5 3.715864366821873E-4 0.0 1.3597225075065622 0.0 86-87 6.193107278036455E-5 3.715864366821873E-4 0.0 1.5117736130278203 0.0 88-89 6.193107278036455E-5 3.819082821455814E-4 0.0 1.6872553077509833 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 20270 0.0 57.7913 1 GTATCAA 38315 0.0 42.599792 1 ATCAACG 46800 0.0 34.460148 3 TCAACGC 47320 0.0 34.073006 4 TATCAAC 48460 0.0 33.385128 2 GTGGTAT 7760 0.0 33.11759 1 CAACGCA 49520 0.0 32.635334 5 AACGCAG 52540 0.0 31.003738 6 TGGTATC 8200 0.0 30.411459 2 CGCAGAG 57830 0.0 27.97182 8 ACGCAGA 58905 0.0 27.541992 7 GCAGAGT 61290 0.0 25.656368 9 GTACATG 39210 0.0 24.848032 1 TACATGG 38280 0.0 24.171791 2 ACATGGG 39435 0.0 22.82709 3 GAGTACT 39380 0.0 22.714685 12-13 CAGAGTA 59785 0.0 22.264307 10-11 GTACTTT 41265 0.0 21.375608 14-15 AGAGTAC 57375 0.0 21.166855 10-11 TATAACG 975 0.0 20.461452 2 >>END_MODULE