FastQCFastQC Report
Wed 25 May 2016
SRR2049402_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049402_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9688190
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343150.35419412707636827No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295090.30458733777929625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178780.18453395319455956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA195200.057.942171
GTATCAA369250.042.586691
ATCAACG475800.032.713383
TCAACGC482550.032.2654574
TATCAAC493700.031.5374072
CAACGCA502350.031.0599065
GTGGTAT78850.029.7127481
AACGCAG530100.029.5591016
TGGTATC84150.026.5856292
CGCAGAG600200.025.7819068
ACGCAGA608600.025.5587357
GCAGAGT641050.023.7093859
GTACATG381550.023.1290651
TACATGG368650.022.8441312
AGAGTAC603150.021.49008810-11
GAGTACT409350.021.33186312-13
ACATGGG384050.021.2228133
AGTACTT416300.020.93009812-13
CAGAGTA625500.020.67665510-11
GTACTTT428300.020.17190214-15
Produced by FastQC (version 0.11.5)