##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049402_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9688190 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.070786803314135 33.0 31.0 34.0 30.0 34.0 2 32.254608755608636 34.0 31.0 34.0 30.0 34.0 3 32.320447575862985 34.0 31.0 34.0 30.0 34.0 4 35.95798812781335 37.0 35.0 37.0 35.0 37.0 5 35.84583652880466 37.0 35.0 37.0 35.0 37.0 6 35.87176923656534 37.0 35.0 37.0 35.0 37.0 7 35.79962923931095 37.0 35.0 37.0 35.0 37.0 8 35.79030737423606 37.0 35.0 37.0 35.0 37.0 9 37.39773445813924 39.0 37.0 39.0 34.0 39.0 10-11 37.38975840688508 39.0 37.0 39.0 34.0 39.0 12-13 37.369952694982246 39.0 37.0 39.0 34.0 39.0 14-15 38.59250076639702 40.0 38.0 41.0 34.0 41.0 16-17 38.58286785250908 40.0 38.0 41.0 34.0 41.0 18-19 38.62723191844916 40.0 38.0 41.0 34.0 41.0 20-21 38.62150731973671 40.0 38.0 41.0 34.0 41.0 22-23 38.540831775594825 40.0 38.0 41.0 34.0 41.0 24-25 38.442569922761635 40.0 38.0 41.0 34.0 41.0 26-27 38.26300619620383 40.0 38.0 41.0 34.0 41.0 28-29 38.08481223014825 40.0 38.0 41.0 33.0 41.0 30-31 37.89438945767992 40.0 37.0 41.0 33.0 41.0 32-33 37.64472414351907 40.0 37.0 41.0 33.0 41.0 34-35 37.45775996341938 40.0 36.5 41.0 32.5 41.0 36-37 37.264906551172096 39.5 36.0 41.0 32.0 41.0 38-39 37.411114563194985 40.0 37.0 41.0 33.0 41.0 40-41 37.31548328428737 40.0 37.0 41.0 32.0 41.0 42-43 37.12368822246467 40.0 36.0 41.0 31.5 41.0 44-45 36.875395558922776 40.0 36.0 41.0 31.0 41.0 46-47 36.53243159971058 39.5 36.0 41.0 30.0 41.0 48-49 36.250702143537644 39.0 36.0 41.0 30.0 41.0 50-51 36.01386678006934 39.0 35.0 41.0 29.0 41.0 52-53 35.83194193136179 39.0 35.0 40.5 29.0 41.0 54-55 35.72993242287775 39.0 35.0 40.0 28.5 41.0 56-57 35.63972496410578 39.0 35.0 40.0 28.0 41.0 58-59 35.40520463574724 38.5 35.0 40.0 28.0 41.0 60-61 35.16752540980307 38.0 34.5 40.0 27.5 41.0 62-63 34.895990633957425 38.0 34.0 40.0 27.0 41.0 64-65 34.64925760126505 37.5 34.0 40.0 26.0 41.0 66-67 34.320865920259614 37.0 34.0 40.0 26.0 41.0 68-69 34.010482659815715 36.5 34.0 39.5 26.0 41.0 70-71 33.68537760923351 36.0 33.5 39.0 26.0 41.0 72-73 33.323128778440555 35.5 33.0 39.0 25.0 40.0 74-75 32.953779601762555 35.0 33.0 38.0 24.5 40.0 76-77 30.938522933592346 33.5 30.0 35.5 21.0 38.5 78-79 32.25441413721242 35.0 32.5 37.0 24.0 39.0 80-81 32.267000492352025 35.0 33.0 37.0 24.0 39.0 82-83 31.999289237721392 35.0 33.0 36.0 24.0 38.5 84-85 31.732481660661072 35.0 32.5 36.0 23.5 37.0 86-87 31.464333637139653 35.0 32.0 35.5 23.0 37.0 88-89 31.19926116230173 35.0 32.0 35.0 21.0 36.5 90-91 30.93506748938656 35.0 32.0 35.0 20.0 36.0 92-93 30.694109426012496 35.0 32.0 35.0 18.5 36.0 94-95 30.438745988672807 34.0 31.5 35.0 17.5 36.0 96-97 30.203745797718668 34.0 31.0 35.0 9.5 35.0 98-99 29.929660700295926 34.0 31.0 35.0 2.0 35.0 100-101 29.32452341459034 34.0 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3684439760549125 1101 2 0.4004035059899351 1101 3 0.38113250619773 1101 4 0.29476068799095856 1101 5 0.28433812687249826 1101 6 0.3010954536016399 1101 7 0.3584833715463276 1101 8 0.35361113339157413 1101 9 0.4383459583058027 1101 10-11 0.445424713875731 1101 12-13 0.36035841737844976 1101 14-15 0.4401038465418807 1101 16-17 0.4184793432696665 1101 18-19 0.37188061902082836 1101 20-21 0.3196325370389559 1101 22-23 0.3263635389541122 1101 24-25 0.3428076891458076 1101 26-27 0.32454550680905214 1101 28-29 0.3302826416434499 1101 30-31 0.27301062018246824 1101 32-33 0.3696074358454027 1101 34-35 0.3070802007222966 1101 36-37 0.3245149422226774 1101 38-39 0.4673223788126535 1101 40-41 0.4125546994353826 1101 42-43 0.4130571419305795 1101 44-45 0.4148399851663811 1101 46-47 0.4327137090994029 1101 48-49 0.3469949053067012 1101 50-51 0.39697993477543037 1101 52-53 0.29476628881403855 1101 54-55 0.2902547693411819 1101 56-57 0.27836656604924315 1101 58-59 0.2835488152405148 1101 60-61 0.25234681603446063 1101 62-63 0.22345127623876238 1101 64-65 0.1295595865041861 1101 66-67 0.10969397540252857 1101 68-69 0.11535231149252212 1101 70-71 0.14892740284146555 1101 72-73 0.05758917057443824 1101 74-75 0.024926190071873577 1101 76-77 0.04551961529740112 1101 78-79 0.1358835125395501 1101 80-81 0.11091131926509235 1101 82-83 0.06814536888119704 1101 84-85 0.009359053191346334 1101 86-87 -0.042167036388711665 1101 88-89 -0.10381500904029295 1101 90-91 -0.005339931743531423 1101 92-93 -0.07553757174865439 1101 94-95 -0.11886060131250886 1101 96-97 -0.12793884795291888 1101 98-99 -0.21613217370421367 1101 100-101 -0.21680987382820405 1102 1 0.355826332423149 1102 2 0.3670644730476056 1102 3 0.34101301886101254 1102 4 0.30296101162048217 1102 5 0.2937591913254778 1102 6 0.3048100753423242 1102 7 0.29619129794647847 1102 8 0.29776839777252206 1102 9 0.40334902810894135 1102 10-11 0.350338688366314 1102 12-13 0.2982944903134026 1102 14-15 0.3743280323405429 1102 16-17 0.39813090031314147 1102 18-19 0.3701011632883038 1102 20-21 0.35051507876750776 1102 22-23 0.3375820102236844 1102 24-25 0.34525500349698035 1102 26-27 0.3340143233825543 1102 28-29 0.3228422640161668 1102 30-31 0.23254224429969383 1102 32-33 0.2579143591635855 1102 34-35 0.23003017741496734 1102 36-37 0.23112971761792522 1102 38-39 0.39481375608491476 1102 40-41 0.37144110790573137 1102 42-43 0.45641651473737 1102 44-45 0.46834252575366975 1102 46-47 0.46608614447946906 1102 48-49 0.290550059011494 1102 50-51 0.3540313398129271 1102 52-53 0.36951800854466654 1102 54-55 0.3390144881196093 1102 56-57 0.36536930787880806 1102 58-59 0.36848019210476224 1102 60-61 0.4134341907048551 1102 62-63 0.34947901494730615 1102 64-65 0.3307928159135969 1102 66-67 0.32699945907732797 1102 68-69 0.2853519352467444 1102 70-71 0.2312211167565721 1102 72-73 0.16267371503553818 1102 74-75 0.19855954943596288 1102 76-77 0.14000329525665833 1102 78-79 0.21002437533374518 1102 80-81 0.2660883134868115 1102 82-83 0.2895961772850555 1102 84-85 0.3043517341820632 1102 86-87 0.2508028985540989 1102 88-89 0.1991296386969168 1102 90-91 0.27948370618133467 1102 92-93 0.21775040495524323 1102 94-95 0.19062137267862056 1102 96-97 0.11382735328072258 1102 98-99 0.1342613274799227 1102 100-101 0.18128449500955668 1103 1 0.35298504702453215 1103 2 0.32883881804672654 1103 3 0.28680835516187386 1103 4 0.26880359003731513 1103 5 0.2672654443329492 1103 6 0.2818360809631528 1103 7 0.2594653278325154 1103 8 0.2685996712104597 1103 9 0.3518452026376764 1103 10-11 0.3282526056711177 1103 12-13 0.28503627632923667 1103 14-15 0.36544475678878285 1103 16-17 0.4405865519676482 1103 18-19 0.3837381079810811 1103 20-21 0.3431483362182064 1103 22-23 0.28984420243180864 1103 24-25 0.34548005913148216 1103 26-27 0.30070604149089064 1103 28-29 0.3215866901956943 1103 30-31 0.29292414160095603 1103 32-33 0.30437461594123505 1103 34-35 0.33452605012783465 1103 36-37 0.35233982200011127 1103 38-39 0.4999121195421452 1103 40-41 0.44447487897230076 1103 42-43 0.46766209869167596 1103 44-45 0.4866091353244215 1103 46-47 0.523413035903836 1103 48-49 0.49462909121820076 1103 50-51 0.461713644367272 1103 52-53 0.40811714254726184 1103 54-55 0.4256383703831048 1103 56-57 0.42050754911938526 1103 58-59 0.40070397252886636 1103 60-61 0.44754594643055157 1103 62-63 0.4904985844173808 1103 64-65 0.4527482308094335 1103 66-67 0.38536775598257833 1103 68-69 0.362496358594079 1103 70-71 0.3539133221379984 1103 72-73 0.4063356245834626 1103 74-75 0.39188370438513687 1103 76-77 0.3916264459067307 1103 78-79 0.48083964092926124 1103 80-81 0.46363493675443834 1103 82-83 0.525065370751058 1103 84-85 0.48853264609036273 1103 86-87 0.4899810607006607 1103 88-89 0.4084483601928106 1103 90-91 0.4553625225487572 1103 92-93 0.40324888215218024 1103 94-95 0.4570000762596358 1103 96-97 0.45801398139207805 1103 98-99 0.4234714882499695 1103 100-101 0.43510554631191667 1104 1 0.30156989615269225 1104 2 0.29016449406481826 1104 3 0.29570962881526697 1104 4 0.2337858896455245 1104 5 0.2513257764392378 1104 6 0.25582433132819915 1104 7 0.272591061371962 1104 8 0.2794763370544544 1104 9 0.3828970308077615 1104 10-11 0.3044541109499548 1104 12-13 0.24172592680628213 1104 14-15 0.3104225596370114 1104 16-17 0.3470969468124778 1104 18-19 0.2998614942985043 1104 20-21 0.2998539497972246 1104 22-23 0.31747285695841754 1104 24-25 0.3040531941352853 1104 26-27 0.3335942984641491 1104 28-29 0.33388722930882153 1104 30-31 0.30366719461423486 1104 32-33 0.3342853532342005 1104 34-35 0.30558797318856534 1104 36-37 0.32007698102351156 1104 38-39 0.41500914717296666 1104 40-41 0.3983736280173531 1104 42-43 0.39535313331136734 1104 44-45 0.40368234704907024 1104 46-47 0.49053909570741183 1104 48-49 0.3998999907896348 1104 50-51 0.4340946281365703 1104 52-53 0.36635987155749206 1104 54-55 0.3671612661498145 1104 56-57 0.33534632772868633 1104 58-59 0.37177897241268454 1104 60-61 0.38308457535556073 1104 62-63 0.3545393861060404 1104 64-65 0.36731445404015517 1104 66-67 0.35806437049831885 1104 68-69 0.35496525987689864 1104 70-71 0.26294318316252685 1104 72-73 0.2813405244847118 1104 74-75 0.29722473949143335 1104 76-77 0.2832298980296457 1104 78-79 0.4374894201205848 1104 80-81 0.3698095750489898 1104 82-83 0.32731663076808104 1104 84-85 0.31911328037609366 1104 86-87 0.2551523584347919 1104 88-89 0.29303402839898496 1104 90-91 0.24599799488287388 1104 92-93 0.22198255032026282 1104 94-95 0.23324402546771594 1104 96-97 0.2590755162118228 1104 98-99 0.26719659604831847 1104 100-101 0.2780036987412444 1105 1 0.28177033356352155 1105 2 0.25280934403775746 1105 3 0.24863667880507734 1105 4 0.21590733993423328 1105 5 0.24895578821971753 1105 6 0.23572452979775704 1105 7 0.21653483438189625 1105 8 0.20653337540476002 1105 9 0.2851887720833872 1105 10-11 0.24337288235245325 1105 12-13 0.23946287553035717 1105 14-15 0.30561712811724107 1105 16-17 0.28580738685379714 1105 18-19 0.2941416996616937 1105 20-21 0.24349004602137825 1105 22-23 0.20557889734463686 1105 24-25 0.19374408961120082 1105 26-27 0.20203571269386345 1105 28-29 0.21100108828343878 1105 30-31 0.21148819466952062 1105 32-33 0.258169351791544 1105 34-35 0.2657911714125305 1105 36-37 0.18508786875749905 1105 38-39 0.3225130450747571 1105 40-41 0.3493319543277593 1105 42-43 0.3330808865647512 1105 44-45 0.36302975262029946 1105 46-47 0.4054232819115313 1105 48-49 0.3900794030736989 1105 50-51 0.3419101471356356 1105 52-53 0.3940826338737722 1105 54-55 0.3950358998257357 1105 56-57 0.3786667315065273 1105 58-59 0.3481166072789321 1105 60-61 0.3701474385831034 1105 62-63 0.3856343400879041 1105 64-65 0.35294955846974574 1105 66-67 0.3642967309738836 1105 68-69 0.296984782600056 1105 70-71 0.2547816240821277 1105 72-73 0.3226679854861274 1105 74-75 0.2827701492533592 1105 76-77 0.28859511443137364 1105 78-79 0.34347138750709405 1105 80-81 0.30038345372425823 1105 82-83 0.37889439429306293 1105 84-85 0.36747318238669635 1105 86-87 0.38341743386990146 1105 88-89 0.3823983164429734 1105 90-91 0.42199181077618775 1105 92-93 0.4011835524745351 1105 94-95 0.433566723421027 1105 96-97 0.4498843299633215 1105 98-99 0.37837152304577515 1105 100-101 0.45185205506571435 1106 1 0.28538451979579804 1106 2 0.2474775004842371 1106 3 0.24082268565610576 1106 4 0.20233760206686924 1106 5 0.21205188391846974 1106 6 0.23411713578529714 1106 7 0.2248609393427614 1106 8 0.24214597384538905 1106 9 0.32739756668489406 1106 10-11 0.2527244360849963 1106 12-13 0.272717064310946 1106 14-15 0.2861850576663443 1106 16-17 0.3426444470869754 1106 18-19 0.3196566293137053 1106 20-21 0.30747094351571036 1106 22-23 0.2866763358054669 1106 24-25 0.31039899485826794 1106 26-27 0.3125942821131886 1106 28-29 0.4229075653952279 1106 30-31 0.42598585953766843 1106 32-33 0.36144319194405483 1106 34-35 0.35698627866197086 1106 36-37 0.4298050311190025 1106 38-39 0.43422202834904056 1106 40-41 0.46991223336744525 1106 42-43 0.5701212723393141 1106 44-45 0.5049227040995419 1106 46-47 0.5704972563402535 1106 48-49 0.4648465024625992 1106 50-51 0.4968460228958378 1106 52-53 0.4944418472308243 1106 54-55 0.529489753405862 1106 56-57 0.4755514254890869 1106 58-59 0.5347078538423986 1106 60-61 0.5707433505096802 1106 62-63 0.5507462941007546 1106 64-65 0.5618703502093112 1106 66-67 0.5366088790501564 1106 68-69 0.5897865487732261 1106 70-71 0.5673928062518172 1106 72-73 0.63678977498612 1106 74-75 0.508306396478396 1106 76-77 0.518282162658096 1106 78-79 0.542115638868232 1106 80-81 0.5938982559608874 1106 82-83 0.558645640268459 1106 84-85 0.5350427830374969 1106 86-87 0.504560055784367 1106 88-89 0.5339295062575857 1106 90-91 0.5069974709593694 1106 92-93 0.5035226047981496 1106 94-95 0.5940476184650585 1106 96-97 0.5507507545407648 1106 98-99 0.6187516619285347 1106 100-101 0.6912618768311987 1107 1 0.21399466871329054 1107 2 0.21001314866353482 1107 3 0.189946582710931 1107 4 0.15601344284131358 1107 5 0.18453798097331742 1107 6 0.18533583017347866 1107 7 0.19907632451050006 1107 8 0.1434445008307108 1107 9 0.24059577024372203 1107 10-11 0.23776520239795929 1107 12-13 0.1758038170389682 1107 14-15 0.21035456537917696 1107 16-17 0.2576453091665485 1107 18-19 0.2256337641787809 1107 20-21 0.21754526591141854 1107 22-23 0.20518183885360486 1107 24-25 0.2013634760792513 1107 26-27 0.2531729699774914 1107 28-29 0.2763200682963003 1107 30-31 0.24872006659776247 1107 32-33 0.2782483481582503 1107 34-35 0.3063471983801733 1107 36-37 0.34987433248299027 1107 38-39 0.41998487275670016 1107 40-41 0.41466876875802683 1107 42-43 0.3693538887497567 1107 44-45 0.36324730885824863 1107 46-47 0.3179189962798432 1107 48-49 0.31838117663382093 1107 50-51 0.3827955958496787 1107 52-53 0.36821333587803196 1107 54-55 0.35620654901291715 1107 56-57 0.39931694666972817 1107 58-59 0.4001475960551133 1107 60-61 0.41926838167695024 1107 62-63 0.47481364662071 1107 64-65 0.4817514802541325 1107 66-67 0.4235765307774031 1107 68-69 0.4366102689020863 1107 70-71 0.4297982575469206 1107 72-73 0.41340191705057094 1107 74-75 0.4259541512989884 1107 76-77 0.3784273015818371 1107 78-79 0.4671410774598641 1107 80-81 0.5094744048375404 1107 82-83 0.5218438483706578 1107 84-85 0.5437719079221779 1107 86-87 0.5289242813104416 1107 88-89 0.5624617302893142 1107 90-91 0.6207335219116068 1107 92-93 0.6342714834359136 1107 94-95 0.6737166845066156 1107 96-97 0.6822056125921456 1107 98-99 0.719202166531069 1107 100-101 0.790720952060564 1108 1 0.1553977928286301 1108 2 0.14596209033144447 1108 3 0.1478823607929982 1108 4 0.13708869723230777 1108 5 0.09876555435642587 1108 6 0.13981807300821458 1108 7 0.1665546486402789 1108 8 0.14358487906599038 1108 9 0.2106510281611378 1108 10-11 0.11516334289532182 1108 12-13 0.13510376503859334 1108 14-15 0.20077424168781732 1108 16-17 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pass #Quality Count 2 5.0 3 22.0 4 84.0 5 166.0 6 436.0 7 1251.0 8 3271.0 9 6552.0 10 11062.0 11 16547.0 12 23257.0 13 33252.0 14 41627.0 15 52607.0 16 66190.0 17 88109.0 18 74931.0 19 43158.0 20 34912.0 21 33758.0 22 35171.0 23 38255.0 24 45541.0 25 56741.0 26 71864.0 27 91117.0 28 115926.0 29 146847.0 30 186192.0 31 234863.0 32 301257.0 33 397779.0 34 555105.0 35 822222.0 36 1300728.0 37 2027014.0 38 2203613.0 39 526595.0 40 163.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.65951295762075 22.16389199335508 16.56704376722445 22.60955128179972 2 14.212995353213763 21.76435845386234 39.86772895832013 24.154917234603765 3 16.485865333025878 26.969325257226185 31.987643481748762 24.55716592799917 4 11.927419890910187 18.63696774744936 38.96138628536841 30.47422607627204 5 12.416113374925306 38.16128052988012 35.51393399227719 13.908672102917375 6 28.86862075966708 39.49453174424833 17.85046191647054 13.786385579614052 7 25.78698660812666 33.94917254401209 22.671502382953097 17.592338464908146 8 25.388849723219714 35.83576498809375 20.273869525680237 18.5015157630063 9 24.997700807261793 16.824640905317334 22.011299745266992 36.16635854215388 10-11 23.464622431420864 27.63066505632065 28.77114675841827 20.13356575384022 12-13 24.5024625086039 25.12540139723733 29.18023021742091 21.191905876737856 14-15 21.677480227378947 26.883688970766745 27.31182050968689 24.127010292167416 16-17 20.354142517849052 30.414855612864734 27.923384037678865 21.30761783160735 18-19 20.40394152278995 28.3540997799949 31.154856722639828 20.08710197457532 20-21 21.519515064986393 27.31731832832575 31.049483319482214 20.113683287205646 22-23 21.11134277919818 27.228006469732733 31.22594106845551 20.434709682613576 24-25 20.899011806964964 27.43615581426255 30.972010874123125 20.692821504649373 26-27 20.62323988968999 27.666370182666178 30.927613327194102 20.782776600449726 28-29 20.66806087953915 27.554937067645064 31.166278388411868 20.61072366440392 30-31 20.93560322460494 27.789873122826673 30.787755931379728 20.486767721188652 32-33 20.650037584570445 27.59658697829344 31.167864397429838 20.58551103970628 34-35 20.968479427056142 27.56956827762825 30.813213883662872 20.648738411652737 36-37 21.199985404186613 27.493986065540057 30.52535276785498 20.78067576241835 38-39 20.850773448236637 27.78544999470474 30.70929407507127 20.654482481987348 40-41 20.76935864380789 27.661570471074022 30.709347672478838 20.859723212639253 42-43 20.860578221265445 27.718228507349668 30.48098607192196 20.940207199462925 44-45 20.986807221186645 27.941269823411254 30.177770612058936 20.89415234334317 46-47 21.274052269589173 28.095304155533185 29.680792525360182 20.949851049517456 48-49 21.317360924310677 28.01854934321828 29.47760724145484 21.186482491016204 50-51 21.264579298294436 28.226644530809704 29.119068518624797 21.389707652271063 52-53 21.230227369860273 28.398733007489746 29.076361262663436 21.294678359986545 54-55 21.324952055880093 28.66930120416265 28.99153668032896 21.014210059628297 56-57 21.277902339823658 28.386874254631888 28.934305006747408 21.400918398797046 58-59 21.23364414017253 28.144000723360307 29.21595814584422 21.406396990622948 60-61 21.349416387236946 28.346333473306256 29.056788955104203 21.247461184352595 62-63 21.16395082064258 28.298676708581556 29.183742258985966 21.353630211789902 64-65 21.21854709310757 28.272013228606863 29.14395451324538 21.365485165040184 66-67 21.079794290095723 28.289906615895354 29.13956292477525 21.49073616923367 68-69 21.295967980104624 28.504552836615765 28.828290379424903 21.37118880385471 70-71 21.385520156483054 28.210312811275863 28.750365144328782 21.6538018879123 72-73 21.159683782471784 28.407359058874142 28.902737893678466 21.530219264975603 74-75 21.179486851294723 28.60721041582498 28.7117276711072 21.5015750617731 76-77 21.268702222076797 28.601061737647964 28.62164909086114 21.508586949414102 78-79 21.250656229478313 28.535185357603005 28.875206674867943 21.338951738050742 80-81 21.19775288889696 28.58106043367607 28.752394629959575 21.468792047467385 82-83 21.056361844770272 28.50608677494676 28.983699873758496 21.453851506524472 84-85 20.989888125811884 28.591593927198428 28.724953202970855 21.693564744018833 86-87 21.10672967362216 28.58829898303661 28.64577384539706 21.659197497944174 88-89 20.927146849216513 28.60801010740407 28.79701298959351 21.667830053785902 90-91 20.869501939239736 28.546847541952346 28.752352763577903 21.83129775523001 92-93 20.933111035016115 28.582399227099614 28.721935203016663 21.762554534867608 94-95 20.83179494113682 28.426644109888944 28.893281427972024 21.848279521002215 96-97 20.874493731553205 28.60792892111515 28.70489774354082 21.81267960379083 98-99 20.640005326109367 28.485674081846096 28.757625639023455 22.11669495302108 100-101 20.612963856662795 28.639860935833262 28.62372272961837 22.123452477885575 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1734.0 1 1227.5 2 1058.0 3 1498.5 4 1312.0 5 1148.5 6 1958.0 7 4385.0 8 7712.0 9 9826.5 10 9629.5 11 9107.0 12 9804.0 13 10888.5 14 11836.5 15 12683.0 16 13562.0 17 14436.5 18 15605.0 19 17181.0 20 19401.5 21 22715.0 22 29193.0 23 40830.5 24 53043.0 25 65207.0 26 84715.5 27 111461.0 28 147743.5 29 180224.5 30 196509.5 31 217050.0 32 245179.0 33 269174.0 34 288656.0 35 307840.0 36 322823.0 37 330028.0 38 336058.0 39 340828.5 40 345705.0 41 344720.5 42 336439.0 43 329308.5 44 322526.0 45 313231.5 46 304717.0 47 295825.0 48 285984.0 49 282260.0 50 277488.5 51 262404.0 52 239640.0 53 223753.5 54 216881.0 55 208197.0 56 189040.0 57 166338.5 58 151012.0 59 137982.5 60 123307.5 61 107276.0 62 91268.0 63 76464.0 64 63185.0 65 52061.0 66 42164.0 67 32682.5 68 25026.0 69 19190.5 70 14592.0 71 10825.0 72 8101.0 73 6214.0 74 4949.0 75 3762.5 76 2678.5 77 2020.0 78 1525.5 79 1062.0 80 666.0 81 400.5 82 278.0 83 193.5 84 127.5 85 91.5 86 59.5 87 41.0 88 35.5 89 25.0 90 18.5 91 14.0 92 7.0 93 5.0 94 5.5 95 5.0 96 2.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05169180208067761 2 0.0021056564745323947 3 2.477242911214582E-4 4 2.6836798204824637E-4 5 0.002714645356872646 6 0.0018888977198011185 7 1.0321845463394092E-5 8 0.0 9 7.225291824375864E-5 10-11 3.096553639018228E-5 12-13 5.160922731697046E-6 14-15 4.7480489131612817E-4 16-17 0.0 18-19 5.6770150048667495E-5 20-21 4.799658140478253E-4 22-23 0.0 24-25 5.418968868281898E-4 26-27 0.007891050856764783 28-29 0.007891050856764783 30-31 0.001233460532875594 32-33 0.007266579206229439 34-35 0.008448430511788065 36-37 0.004959646745160861 38-39 0.0028488293478967693 40-41 0.0021830703155078504 42-43 0.0013831272920948082 44-45 8.98000555315286E-4 46-47 7.328510279009804E-4 48-49 0.0019817943289716657 50-51 0.0014244146739483847 52-53 0.0021521047791176677 54-55 9.134833235103771E-4 56-57 2.477242911214582E-4 58-59 7.431728733643746E-4 60-61 0.006213750968963243 62-63 0.002719806279604343 64-65 9.805753190224385E-4 66-67 0.00585248637774445 68-69 0.007359475815399987 70-71 0.002477242911214582 72-73 5.057704277063105E-4 74-75 0.0032617031664325326 76-77 0.0021004955518006975 78-79 0.0027662545841896165 80-81 0.0010992765418514708 82-83 1.3934491375582024E-4 84-85 7.74138409754557E-4 86-87 0.004918359363307285 88-89 0.0025030475248730673 90-91 0.0011870122282903203 92-93 7.741384097545568E-5 94-95 4.6448304585273406E-4 96-97 2.5288521385315524E-4 98-99 6.967245687791011E-4 100-101 5.160922731697046E-6 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9688190.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.889991386546576 #Duplication Level Percentage of deduplicated Percentage of total 1 64.77718644469614 27.782929686577397 2 18.236268652371056 15.64306810845888 3 6.993404888123542 8.998412262427543 4 3.30932564789947 5.677477941347437 5 1.795176101610874 3.8497543767712328 6 1.0966497548953982 2.8221179124913225 7 0.7485240511184718 2.2472933073565944 8 0.4991201169177303 1.712580601236286 9 0.364196808115869 1.405835816678764 >10 1.9531571619994705 15.117754005710749 >50 0.1396809607382553 4.122553446167813 >100 0.07931212921812425 6.227354202931143 >500 0.005332883442206317 1.541775456893136 >1k 0.00256751167814977 1.9254886175235506 >5k 2.4221793843105005E-5 0.07682462354154869 >10k+ 7.266538152931501E-5 0.8487796338866276 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34315 0.35419412707636827 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29509 0.30458733777929625 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17878 0.18453395319455956 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 1.0321845463394092E-5 0.0 0.0 0.0 4 0.0 2.0643690926788183E-5 0.0 0.0 0.0 5 0.0 2.0643690926788183E-5 0.0 0.0 0.0 6 0.0 2.0643690926788183E-5 0.0 0.0 0.0 7 0.0 2.0643690926788183E-5 0.0 0.0 0.0 8 0.0 2.0643690926788183E-5 0.0 0.0 0.0 9 1.0321845463394092E-5 2.0643690926788183E-5 0.0 0.0 0.0 10-11 1.0321845463394092E-5 2.0643690926788183E-5 0.0 0.0 0.0 12-13 1.0321845463394092E-5 2.0643690926788183E-5 0.0 0.0 0.0 14-15 1.0321845463394092E-5 2.5804613658485232E-5 0.0 0.0 0.0 16-17 1.0321845463394092E-5 3.612645912187932E-5 0.0 0.0 0.0 18-19 1.0321845463394092E-5 6.193107278036455E-5 0.0 5.160922731697046E-6 0.0 20-21 1.0321845463394092E-5 6.193107278036455E-5 0.0 2.5804613658485232E-5 0.0 22-23 1.0321845463394092E-5 7.74138409754557E-5 0.0 5.67701500486675E-5 0.0 24-25 1.0321845463394092E-5 9.289660917054682E-5 0.0 1.7547137287769954E-4 0.0 26-27 2.0643690926788183E-5 9.289660917054682E-5 0.0 2.941725957067316E-4 0.0 28-29 4.128738185357637E-5 1.13540300097335E-4 0.0 7.741384097545568E-4 0.0 30-31 4.128738185357637E-5 1.238621455607291E-4 0.0 0.0018682540288743304 0.0 32-33 4.128738185357637E-5 1.3418399102412318E-4 0.0 0.004520968312966612 0.0 34-35 5.160922731697046E-5 1.445058364875173E-4 0.0 0.008804534180275159 0.0 36-37 5.160922731697046E-5 1.496667592192143E-4 0.0 0.015400193431383984 0.0 38-39 5.160922731697046E-5 1.7031045014600252E-4 0.0 0.025407222608144556 0.0 40-41 5.160922731697046E-5 1.7547137287769954E-4 0.0 0.0379018165415831 0.0 42-43 5.160922731697046E-5 2.0643690926788183E-4 0.0 0.04966872036985237 0.0 44-45 5.160922731697046E-5 2.167587547312759E-4 0.0 0.06439799384611573 0.0 46-47 5.160922731697046E-5 2.7868982751164047E-4 0.0 0.08431915559046632 0.0 48-49 5.67701500486675E-5 2.993335184384286E-4 0.0 0.10738331927842043 0.0 50-51 6.193107278036455E-5 2.993335184384286E-4 0.0 0.13127839152617776 0.0 52-53 6.193107278036455E-5 2.993335184384286E-4 0.0 0.1553024868422275 0.0 54-55 6.193107278036455E-5 2.993335184384286E-4 0.0 0.18207219305154007 0.0 56-57 6.709199551206159E-5 3.45781823023702E-4 0.0 0.21597945539878965 0.0 58-59 7.225291824375864E-5 3.612645912187932E-4 0.0 0.2541599617678844 0.0 60-61 8.257476370715273E-5 3.612645912187932E-4 0.0 0.29664467769521446 0.0 62-63 8.257476370715273E-5 3.664255139504902E-4 0.0 0.3407963716648827 0.0 64-65 8.257476370715273E-5 3.715864366821873E-4 0.0 0.38899938997893313 0.0 66-67 8.257476370715273E-5 3.922301276089755E-4 0.0 0.4468946211831106 0.0 68-69 9.289660917054682E-5 4.5416120038934E-4 0.0 0.5131557081353689 0.0 70-71 9.289660917054682E-5 4.748048913161282E-4 0.0 0.5862498567843942 0.0 72-73 9.289660917054682E-5 4.954485822429164E-4 0.0 0.6656816185479434 0.0 74-75 9.289660917054682E-5 5.006095049746135E-4 0.0 0.7497788544609467 0.0 76-77 9.289660917054682E-5 5.367359640964927E-4 0.0 0.8433257398956874 0.0 78-79 9.805753190224388E-5 5.367359640964927E-4 0.0 0.9531501756262006 0.0 80-81 1.0321845463394091E-4 5.67701500486675E-4 0.0 1.0796960010074121 0.0 82-83 1.13540300097335E-4 5.986670368768572E-4 0.0 1.2131626237718294 0.0 84-85 1.13540300097335E-4 5.986670368768572E-4 0.0 1.3510882837764329 0.0 86-87 1.13540300097335E-4 6.089888823402514E-4 0.0 1.5022207450514493 0.0 88-89 1.13540300097335E-4 6.089888823402514E-4 0.0 1.676887013983004 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 19520 0.0 57.94217 1 GTATCAA 36925 0.0 42.58669 1 ATCAACG 47580 0.0 32.71338 3 TCAACGC 48255 0.0 32.265457 4 TATCAAC 49370 0.0 31.537407 2 CAACGCA 50235 0.0 31.059906 5 GTGGTAT 7885 0.0 29.712748 1 AACGCAG 53010 0.0 29.559101 6 TGGTATC 8415 0.0 26.585629 2 CGCAGAG 60020 0.0 25.781906 8 ACGCAGA 60860 0.0 25.558735 7 GCAGAGT 64105 0.0 23.709385 9 GTACATG 38155 0.0 23.129065 1 TACATGG 36865 0.0 22.844131 2 AGAGTAC 60315 0.0 21.490088 10-11 GAGTACT 40935 0.0 21.331863 12-13 ACATGGG 38405 0.0 21.222813 3 AGTACTT 41630 0.0 20.930098 12-13 CAGAGTA 62550 0.0 20.676655 10-11 GTACTTT 42830 0.0 20.171902 14-15 >>END_MODULE