FastQCFastQC Report
Wed 25 May 2016
SRR2049401_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049401_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6774011
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313080.4621781688869416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255040.37649776476595626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143080.21121902518315958No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72530.10707098054609004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135000.059.962431
GTATCAA259600.044.0651441
TATAACG6950.039.6383972
ATCAACG327050.034.65363
TCAACGC331050.034.2220574
TATCAAC341050.033.201752
CAACGCA344400.032.867195
AACGCAG358950.031.7204256
TACCTGG57050.031.6374032
GTGGTAT55600.030.2462731
GTACCTG61300.030.2236941
ATAACGC9850.029.898373
TAACGCA11200.028.8404624
TGGTATC54850.028.5765742
ACGCAGA402600.028.1061767
CGCAGAG405450.027.8031648
GTACATG249150.025.340051
GCAGAGT448000.024.5792839
TACATGG239950.024.4664292
ACATGGG251650.022.7070523
Produced by FastQC (version 0.11.5)