##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049401_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6774011 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.378948011746658 31.0 31.0 34.0 30.0 34.0 2 31.400668082765144 31.0 31.0 34.0 28.0 34.0 3 31.419895243748496 33.0 31.0 34.0 28.0 34.0 4 35.042155969336335 37.0 35.0 37.0 32.0 37.0 5 35.07179468707683 37.0 35.0 37.0 32.0 37.0 6 35.16374685544503 37.0 35.0 37.0 32.0 37.0 7 35.10955473795363 37.0 35.0 37.0 32.0 37.0 8 35.08325776264609 37.0 35.0 37.0 32.0 37.0 9 36.6480714306487 39.0 37.0 39.0 32.0 39.0 10-11 36.57450571013244 39.0 37.0 39.0 32.0 39.0 12-13 36.52893610594963 39.0 37.0 39.0 32.0 39.0 14-15 37.66949669848484 40.0 38.0 41.0 32.0 41.0 16-17 37.65535529836016 40.0 38.0 41.0 32.0 41.0 18-19 37.691156170251276 40.0 38.0 41.0 32.0 41.0 20-21 37.64558368741946 40.0 38.0 41.0 32.0 41.0 22-23 37.52969296920244 40.0 37.5 41.0 32.0 41.0 24-25 37.411428325109014 40.0 37.0 41.0 32.0 41.0 26-27 37.26149204658805 40.0 37.0 41.0 32.0 41.0 28-29 37.09658096215078 40.0 37.0 41.0 31.0 41.0 30-31 36.88454794360387 40.0 36.5 41.0 31.0 41.0 32-33 36.61317748081602 39.0 36.0 41.0 30.0 41.0 34-35 36.39547588570494 39.0 35.5 41.0 30.0 41.0 36-37 36.19002582074343 39.0 35.0 41.0 30.0 41.0 38-39 35.981024462463964 39.0 35.0 40.5 29.5 41.0 40-41 35.751527049483684 39.0 35.0 40.0 28.5 41.0 42-43 35.507992605857886 39.0 35.0 40.0 27.5 41.0 44-45 35.21077792758234 38.0 35.0 40.0 26.5 41.0 46-47 34.83054360260118 38.0 34.0 40.0 24.5 41.0 48-49 34.50871492532268 38.0 34.0 40.0 24.0 41.0 50-51 33.13587112568905 36.5 32.5 39.0 21.5 40.0 52-53 33.43361584443839 37.5 32.5 39.0 22.5 40.0 54-55 34.47879417379157 38.0 34.0 40.0 24.0 41.0 56-57 34.5762763745143 38.0 34.0 40.0 24.0 41.0 58-59 34.40998678626297 38.0 34.0 40.0 23.0 41.0 60-61 34.14132587030048 38.0 34.0 40.0 22.5 41.0 62-63 33.85409729922198 37.5 34.0 40.0 22.0 41.0 64-65 33.563289238827636 37.0 33.0 40.0 21.5 41.0 66-67 33.23269441103653 36.5 33.0 40.0 20.0 41.0 68-69 32.87793900836594 36.0 33.0 39.0 19.0 41.0 70-71 32.53718085193543 35.5 32.5 39.0 18.0 40.5 72-73 32.27760369742535 35.0 32.0 39.0 17.5 40.0 74-75 32.07896311358219 35.0 32.0 38.0 16.0 40.0 76-77 31.74368177140545 35.0 32.0 37.0 12.5 39.5 78-79 31.37642646284454 35.0 32.0 37.0 9.0 39.0 80-81 31.04753638575432 35.0 32.0 36.0 7.0 39.0 82-83 30.714132734653074 35.0 31.0 36.0 3.5 37.5 84-85 30.40534751124555 35.0 31.0 35.5 2.0 37.0 86-87 30.113519301932044 35.0 31.0 35.0 2.0 37.0 88-89 29.83056390076721 34.5 31.0 35.0 2.0 36.0 90-91 29.566890428728268 34.0 31.0 35.0 2.0 36.0 92-93 29.31303049256932 34.0 30.0 35.0 2.0 36.0 94-95 29.041791857143426 34.0 30.0 35.0 2.0 35.5 96-97 28.776736116903265 34.0 30.0 35.0 2.0 35.0 98-99 28.44641727035873 34.0 29.5 35.0 2.0 35.0 100-101 27.89774426111797 34.0 28.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17552935639138312 1101 2 0.2924810368137116 1101 3 0.19613046821245916 1101 4 0.2604780913354645 1101 5 0.2272784318006913 1101 6 0.2855001677832192 1101 7 0.22778874804112093 1101 8 0.22367416354711622 1101 9 0.1950133803397165 1101 10-11 0.21604798635413403 1101 12-13 0.2776329083258986 1101 14-15 0.39490320081993247 1101 16-17 0.382145597776983 1101 18-19 0.36560341015988485 1101 20-21 0.25831745661010785 1101 22-23 0.3000287724814612 1101 24-25 0.31088147377824527 1101 26-27 0.3390363188372447 1101 28-29 0.27789259636488595 1101 30-31 0.22110020004698328 1101 32-33 0.16963048540509362 1101 34-35 0.2555964692278607 1101 36-37 0.2337807857716072 1101 38-39 0.10654154496362622 1101 40-41 0.08272305752488052 1101 42-43 0.03173712900375847 1101 44-45 0.024245245410014604 1101 46-47 -0.0484272125162164 1101 48-49 -0.13702403382025352 1101 50-51 -0.07133337765299075 1101 52-53 0.04934228734501289 1101 54-55 0.246073048460552 1101 56-57 0.2003676198918498 1101 58-59 0.2420772827776787 1101 60-61 0.29483555407576745 1101 62-63 0.18624190071537328 1101 64-65 0.23993966788834342 1101 66-67 0.24723470345524134 1101 68-69 0.2806730695380111 1101 70-71 0.12923284475736097 1101 72-73 0.15108055613090698 1101 74-75 0.07071488723458685 1101 76-77 0.072733663606936 1101 78-79 0.06402311619216405 1101 80-81 -0.07220625798066749 1101 82-83 -0.1429818596237844 1101 84-85 -0.2441260104754015 1101 86-87 -0.22909031481507824 1101 88-89 -0.31098055665925983 1101 90-91 -0.3587394022815431 1101 92-93 -0.4551062510773072 1101 94-95 -0.5526010644805872 1101 96-97 -0.5390687830511247 1101 98-99 -0.6942214190042542 1101 100-101 -0.843415145775122 1102 1 0.2766195388811532 1102 2 0.2503450826162137 1102 3 0.25706852123493107 1102 4 0.30144038984111177 1102 5 0.32651142538097844 1102 6 0.30936981458254564 1102 7 0.29012782354777045 1102 8 0.33747082235377945 1102 9 0.42865060173692626 1102 10-11 0.3559371167840766 1102 12-13 0.3237516705280754 1102 14-15 0.4220420100092639 1102 16-17 0.3564578354050951 1102 18-19 0.37565600405404354 1102 20-21 0.4209128923258092 1102 22-23 0.3828845339632636 1102 24-25 0.38791784395895235 1102 26-27 0.41813025955430305 1102 28-29 0.33140853485208055 1102 30-31 0.3060762586444312 1102 32-33 0.2983637801320924 1102 34-35 0.33717456898185816 1102 36-37 0.28346659338495783 1102 38-39 0.2154053541882135 1102 40-41 0.268945398640021 1102 42-43 0.2117732488694699 1102 44-45 0.1736973589886972 1102 46-47 0.23964686589091855 1102 48-49 0.18649905658867283 1102 50-51 0.12795067054121745 1102 52-53 0.3206693015630222 1102 54-55 0.4415221555301798 1102 56-57 0.4139794377067574 1102 58-59 0.4595447724194912 1102 60-61 0.4634494549680497 1102 62-63 0.3873388789636252 1102 64-65 0.30360662277582406 1102 66-67 0.3270758591963343 1102 68-69 0.3390772917145597 1102 70-71 0.2653657038371193 1102 72-73 0.30342936176270285 1102 74-75 0.16407454192636095 1102 76-77 0.20672712102912172 1102 78-79 0.20024437926530325 1102 80-81 0.17119994583723042 1102 82-83 0.20060270556207271 1102 84-85 0.13711583569473618 1102 86-87 0.07333075147218082 1102 88-89 0.09568919599741577 1102 90-91 -0.061299583749217845 1102 92-93 -0.134603611489176 1102 94-95 -0.21312310018233305 1102 96-97 -0.3075693712663288 1102 98-99 -0.4161835364261357 1102 100-101 -0.5637077991553703 1103 1 0.30686037193876814 1103 2 0.3030271620801166 1103 3 0.3191513666402379 1103 4 0.33149448519044 1103 5 0.30711487590151876 1103 6 0.3256225481656827 1103 7 0.33647817976850547 1103 8 0.32310512473849684 1103 9 0.4034636644019898 1103 10-11 0.44626448538895147 1103 12-13 0.3832803797907829 1103 14-15 0.43951793416781015 1103 16-17 0.4531105478798878 1103 18-19 0.4071354987521332 1103 20-21 0.3949550055630695 1103 22-23 0.3947288819427044 1103 24-25 0.45708411542272387 1103 26-27 0.4696122573969461 1103 28-29 0.4168504101690971 1103 30-31 0.4516526666979601 1103 32-33 0.3775817739919205 1103 34-35 0.45881719597131365 1103 36-37 0.36605087835876304 1103 38-39 0.42110490324029826 1103 40-41 0.3011753159390409 1103 42-43 0.26837816551753946 1103 44-45 0.23617349058535808 1103 46-47 0.18943130383081552 1103 48-49 0.1495576249635704 1103 50-51 0.051226143682413294 1103 52-53 0.16649777297492818 1103 54-55 0.3193933508632938 1103 56-57 0.36156615986152474 1103 58-59 0.3897510440290759 1103 60-61 0.3551461842503656 1103 62-63 0.29237732965948027 1103 64-65 0.25734614194293925 1103 66-67 0.2297749459825411 1103 68-69 0.253606687519337 1103 70-71 0.23006544192696055 1103 72-73 0.2424280805068122 1103 74-75 0.186635477526103 1103 76-77 0.14160124948522324 1103 78-79 0.17195131641276262 1103 80-81 0.08431921807138565 1103 82-83 0.03968692092693615 1103 84-85 0.1413599632863658 1103 86-87 0.02587886843742382 1103 88-89 -0.03612896530577103 1103 90-91 -0.04612457367241163 1103 92-93 -0.13677806643031687 1103 94-95 -0.08586964082614656 1103 96-97 -0.183740789105034 1103 98-99 -0.15737563992508896 1103 100-101 -0.19892080686234337 1104 1 0.0507145473591919 1104 2 0.07850523762714445 1104 3 0.13844149626239144 1104 4 0.058152784989715656 1104 5 0.05308252340199715 1104 6 0.0405359497105664 1104 7 -0.02301424252226525 1104 8 0.05305994503223843 1104 9 0.017536739942130453 1104 10-11 0.08160668773948032 1104 12-13 0.02261636071873596 1104 14-15 0.05220510379250243 1104 16-17 0.09142690294733313 1104 18-19 0.034805795730008526 1104 20-21 0.07793522678451126 1104 22-23 0.11374549834847159 1104 24-25 0.09276728369181342 1104 26-27 0.10232609468938847 1104 28-29 0.03259559635026932 1104 30-31 0.05710257639365324 1104 32-33 0.14474587778883574 1104 34-35 -0.016057404340855896 1104 36-37 0.021142240004436985 1104 38-39 -0.01859226047431406 1104 40-41 -0.03315321884430489 1104 42-43 -0.013868944537996697 1104 44-45 -0.024785033493728292 1104 46-47 0.06816806643174544 1104 48-49 0.0027638755921230995 1104 50-51 -0.07509174125434015 1104 52-53 0.04425253650989447 1104 54-55 0.13799586588301338 1104 56-57 0.07243084943100087 1104 58-59 0.04024849263966246 1104 60-61 0.03203715023780518 1104 62-63 0.059827552079475765 1104 64-65 0.08594007770908973 1104 66-67 0.12045533141867537 1104 68-69 0.08310332194463399 1104 70-71 0.0965895974680322 1104 72-73 0.0624580028492403 1104 74-75 0.12043126167194629 1104 76-77 0.08332388852010197 1104 78-79 0.10187946215055277 1104 80-81 0.08180373339694569 1104 82-83 0.0494426791180409 1104 84-85 0.026847678869820868 1104 86-87 0.06598059338056217 1104 88-89 0.055227858627695525 1104 90-91 -0.02882968017087606 1104 92-93 -0.06949567501246534 1104 94-95 -0.07619085827423433 1104 96-97 -0.14536352512577722 1104 98-99 -0.19361144851026424 1104 100-101 -0.1961805085481636 1105 1 0.23975000619648412 1105 2 0.2134041503103674 1105 3 0.1870353292852478 1105 4 0.22215586835949352 1105 5 0.2245031993063833 1105 6 0.28500774635512727 1105 7 0.28436517487714497 1105 8 0.2743941859478056 1105 9 0.30568287680342365 1105 10-11 0.30945901055093117 1105 12-13 0.26750184507117325 1105 14-15 0.4139925741004902 1105 16-17 0.33232771437472053 1105 18-19 0.3863147657412682 1105 20-21 0.31253573023130343 1105 22-23 0.2841025058658957 1105 24-25 0.336452370476394 1105 26-27 0.3652287855145744 1105 28-29 0.35214898078516654 1105 30-31 0.26845698717033173 1105 32-33 0.327130428763887 1105 34-35 0.3581753594455961 1105 36-37 0.2889265688186953 1105 38-39 0.27484337908180834 1105 40-41 0.3284624463847905 1105 42-43 0.363658365660136 1105 44-45 0.36906168031359243 1105 46-47 0.40686724848768563 1105 48-49 0.3317017226706227 1105 50-51 0.2865968652963744 1105 52-53 0.41356135273450434 1105 54-55 0.5090424186742482 1105 56-57 0.51832062876192 1105 58-59 0.5058726807073981 1105 60-61 0.4764081654588139 1105 62-63 0.5709178043272587 1105 64-65 0.587139329768334 1105 66-67 0.5475272467888459 1105 68-69 0.5958032365321202 1105 70-71 0.5646064878652552 1105 72-73 0.5885654275439265 1105 74-75 0.5723595260193619 1105 76-77 0.588827293735374 1105 78-79 0.5635479013857321 1105 80-81 0.5180982128600107 1105 82-83 0.5138305770146978 1105 84-85 0.4727527316489173 1105 86-87 0.38816926280697217 1105 88-89 0.3975953092804545 1105 90-91 0.4279299576719069 1105 92-93 0.45301736639097 1105 94-95 0.46732511537669197 1105 96-97 0.40773096881312654 1105 98-99 0.3982183979797007 1105 100-101 0.422195704566672 1106 1 0.2265536365102534 1106 2 0.22813720602222531 1106 3 0.23483934560346142 1106 4 0.24385179426422354 1106 5 0.22572774763375492 1106 6 0.2578590983457758 1106 7 0.29559953315590803 1106 8 0.3417700789911251 1106 9 0.3535276719585454 1106 10-11 0.3498670769767429 1106 12-13 0.3589950500808996 1106 14-15 0.3840040392923356 1106 16-17 0.3615990381831722 1106 18-19 0.43619524888639916 1106 20-21 0.39966858943564887 1106 22-23 0.5043707668033832 1106 24-25 0.42317283520469573 1106 26-27 0.4873888907924311 1106 28-29 0.4974551750207681 1106 30-31 0.4790780951287914 1106 32-33 0.5019691954757945 1106 34-35 0.5527780120434329 1106 36-37 0.45474915581923625 1106 38-39 0.4957446522287938 1106 40-41 0.48943189752129257 1106 42-43 0.5041182064185676 1106 44-45 0.58981891753767 1106 46-47 0.6154501118554876 1106 48-49 0.5972760109251496 1106 50-51 0.5339519826843855 1106 52-53 0.6122685959919565 1106 54-55 0.6398109681167199 1106 56-57 0.6194770341737907 1106 58-59 0.7195436864747364 1106 60-61 0.7040970126222916 1106 62-63 0.6235471508362593 1106 64-65 0.7446454638667745 1106 66-67 0.7486411915009015 1106 68-69 0.7190244578557525 1106 70-71 0.7485069143125926 1106 72-73 0.7619060790938192 1106 74-75 0.7927912403673716 1106 76-77 0.7644559972276177 1106 78-79 0.708250991413724 1106 80-81 0.6965383107875347 1106 82-83 0.6928281195298567 1106 84-85 0.7520995861036042 1106 86-87 0.6750936491874207 1106 88-89 0.728645386938787 1106 90-91 0.6853598459097903 1106 92-93 0.6604510800218186 1106 94-95 0.653006446599079 1106 96-97 0.6869786546299785 1106 98-99 0.658228149826531 1106 100-101 0.6971767196939105 1107 1 0.21974816895908234 1107 2 0.1361300536579364 1107 3 0.1374803510208693 1107 4 0.09636148520250742 1107 5 0.100199723049883 1107 6 0.13395100734462773 1107 7 0.1951033452804367 1107 8 0.20658672424839608 1107 9 0.29576547171560463 1107 10-11 0.2753662616471573 1107 12-13 0.23283150360822447 1107 14-15 0.3318660228414245 1107 16-17 0.21041411347741246 1107 18-19 0.18502026281425543 1107 20-21 0.2548333153531175 1107 22-23 0.3136241422095054 1107 24-25 0.3217979710261787 1107 26-27 0.34612688116496315 1107 28-29 0.32217569024676607 1107 30-31 0.357884286677681 1107 32-33 0.37044663584593707 1107 34-35 0.36534079628434313 1107 36-37 0.3998240789802665 1107 38-39 0.36426715239532115 1107 40-41 0.4152744653732796 1107 42-43 0.38201199359521354 1107 44-45 0.32126046610541437 1107 46-47 0.36006582576190027 1107 48-49 0.3568349957191046 1107 50-51 0.31162091675123804 1107 52-53 0.33324967617862455 1107 54-55 0.46387984225177803 1107 56-57 0.43296659548533967 1107 58-59 0.4629396935398091 1107 60-61 0.48148157478674847 1107 62-63 0.5479855091375399 1107 64-65 0.5014304870506834 1107 66-67 0.562680336443897 1107 68-69 0.5442999708747891 1107 70-71 0.5872115000273155 1107 72-73 0.5430050643093978 1107 74-75 0.5970749702139031 1107 76-77 0.5010460436110584 1107 78-79 0.47880666232984126 1107 80-81 0.5233819581431973 1107 82-83 0.5506750128757254 1107 84-85 0.571430031226587 1107 86-87 0.536786528683475 1107 88-89 0.5871166245495516 1107 90-91 0.6081340943573856 1107 92-93 0.536681127192626 1107 94-95 0.5726363308149196 1107 96-97 0.5814234310242838 1107 98-99 0.6575776799204576 1107 100-101 0.7488333483474783 1108 1 0.13326481920242728 1108 2 0.1678185317047607 1108 3 0.12614968352649925 1108 4 0.16453788786501633 1108 5 0.12546950804711798 1108 6 0.10125192440389696 1108 7 0.13238097445387353 1108 8 0.13564747740090866 1108 9 0.12235344040210805 1108 10-11 0.1623900300395107 1108 12-13 0.16130733811031206 1108 14-15 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#Quality Count 2 67120.0 3 15049.0 4 11995.0 5 10663.0 6 10916.0 7 12023.0 8 13830.0 9 16475.0 10 19476.0 11 22998.0 12 28417.0 13 33530.0 14 35215.0 15 40959.0 16 49852.0 17 63211.0 18 52021.0 19 32833.0 20 30247.0 21 30861.0 22 32635.0 23 35313.0 24 40686.0 25 48252.0 26 57487.0 27 70115.0 28 85610.0 29 105239.0 30 129779.0 31 165625.0 32 212990.0 33 286266.0 34 403127.0 35 609149.0 36 927017.0 37 1347697.0 38 1311454.0 39 307750.0 40 129.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.894679758022235 19.744799180395034 14.570415349491858 21.79010571209087 2 15.923116465303764 20.408481423818152 40.1940955528713 23.474306558006777 3 18.08093632450116 26.650555381680967 30.568136858328955 24.70037143548892 4 12.549678338295752 17.649982815576294 38.63188366086097 31.168455185266986 5 12.8440010770886 37.40243313370189 34.787378340362366 14.96618744884715 6 29.163458709288715 37.87638659421002 18.595380452795528 14.364774243705746 7 26.529784639922195 33.21112426779744 22.622854064660725 17.636237027619643 8 25.988376530494595 34.48906647746456 20.495802225929694 19.02675476611115 9 25.78388616552145 16.96429480990948 21.757221361076102 35.49459766349297 10-11 24.013241327587405 26.932644766582666 28.392815225238394 20.661298680591543 12-13 25.10233081735472 24.77234761105062 28.654230719545275 21.471090852049386 14-15 22.398324714302376 26.203989801838564 27.115321305565438 24.282364178293626 16-17 21.058037624272803 29.567574494294472 27.40743261417921 21.966955267253514 18-19 21.115162366296524 27.65728418405263 30.616948523995102 20.610604925655743 20-21 22.043768002806047 26.808016127679828 30.67883348312298 20.46938238639115 22-23 21.777136758130066 26.817342369938867 30.654516654290788 20.751004217640276 24-25 21.31382120878738 27.010753031062674 30.537679603491945 21.137746156658004 26-27 21.197711018397815 27.038707739724778 30.552694709916896 21.21088653196051 28-29 21.254539725378862 26.92477602249972 30.745390005433315 21.075294246688102 30-31 21.463839826913958 27.294783621780542 30.317958208271307 20.92341834303419 32-33 21.072076117794094 27.17372594488097 30.611785904827364 21.14241203249757 34-35 21.4537199595949 27.01442559471855 30.401581954849476 21.130272490837072 36-37 21.829971825022696 27.088493904968004 29.971714303914343 21.109819966094957 38-39 21.55601775523894 27.410336574800176 30.024381861642823 21.00926380831806 40-41 21.368029482938418 27.188559594769035 30.21130925315952 21.232101669133026 42-43 21.426633297061382 27.414251230729324 29.88551231511749 21.273603157091802 44-45 21.445324789425115 27.588842428287613 29.57449795754945 21.391334824737825 46-47 21.89568603567441 27.64183115097491 29.079046590814716 21.383436222535966 48-49 21.851270432741902 27.65090760064376 28.997484593439303 21.500337373175036 50-51 21.809148360688773 27.816693528278215 28.69280724435297 21.68135086668004 52-53 21.799273448844286 27.844958619360867 28.687498108324185 21.66826982347066 54-55 21.821419713352665 28.270512351110504 28.51254451183144 21.39552342370539 56-57 21.664010164121 27.96633208734961 28.63851224243779 21.7311455060916 58-59 21.72532480052534 27.608133233690737 28.82632159068328 21.84022037510065 60-61 21.903548145729832 27.782390650256566 28.743953739626484 21.57010746438712 62-63 21.706570516747746 27.89321489382872 28.849456130369276 21.550758459054254 64-65 21.891262664224236 27.90513467551825 28.638904387612047 21.56469827264547 66-67 21.73092656668014 27.812390479500127 28.580344164895394 21.876338788924343 68-69 21.90028123549341 28.123099080649798 28.436657353661182 21.53996233019561 70-71 22.052430433968738 27.760349650390943 28.49512360175569 21.692096313884626 72-73 21.659364761971194 28.034818847439464 28.498538696404257 21.807277694185085 74-75 21.72838073659476 27.975490046282047 28.466809597601145 21.829319619522046 76-77 21.864102914073484 28.302425799885185 28.134808276985318 21.698663009056013 78-79 21.87709407637419 28.207875875221504 28.294897920166484 21.620132128237824 80-81 21.77873151136392 28.183870806288418 28.325725102378556 21.711672579969104 82-83 21.71218024140402 28.22949962357675 28.39460466404575 21.66371547097348 84-85 21.67768229977128 28.15987352887854 28.246646737740726 21.915797433609452 86-87 21.814304059683526 28.21803106164681 28.228549848976048 21.739115029693615 88-89 21.603831521699014 28.307178677878476 28.24941207385504 21.839577726567473 90-91 21.60873059803746 28.08533840855061 28.42236332568946 21.88356766772247 92-93 21.719849086552024 28.281272858712104 28.166280850357744 21.832597204378125 94-95 21.808141300805627 28.01763529006664 28.32301987734572 21.851203531782016 96-97 21.782478220009278 28.18565672425751 28.176393197515516 21.8554718582177 98-99 21.56641021157158 28.19167527672444 28.189327917971767 22.05258659373221 100-101 21.732781896330582 28.216315374720637 27.9600263538166 22.090876375132183 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4122.0 1 2758.0 2 3793.5 3 5602.5 4 3601.5 5 2345.5 6 3557.5 7 6749.5 8 11327.0 9 13635.0 10 11098.0 11 8244.0 12 7945.0 13 8617.5 14 9318.5 15 9777.5 16 10303.0 17 10928.0 18 11870.5 19 12919.0 20 14248.0 21 16400.0 22 20053.5 23 26168.0 24 33888.5 25 43122.0 26 55918.0 27 70101.5 28 86562.5 29 104690.0 30 117712.5 31 130352.0 32 145151.5 33 157923.5 34 169131.5 35 180564.5 36 190297.0 37 198846.5 38 210519.5 39 218338.5 40 223847.5 41 229214.0 42 229814.0 43 230406.5 44 229014.5 45 224752.5 46 221382.0 47 215165.5 48 206791.5 49 202582.0 50 200423.0 51 193577.0 52 181461.0 53 170765.0 54 164122.5 55 159119.0 56 150083.5 57 136076.5 58 125098.0 59 116986.5 60 105881.0 61 92314.5 62 78832.5 63 66315.0 64 54997.0 65 46366.5 66 38498.5 67 30686.5 68 23763.0 69 17935.5 70 13976.0 71 10460.0 72 7974.5 73 6300.0 74 4943.5 75 3780.5 76 2825.0 77 2251.0 78 1899.0 79 1664.0 80 1296.0 81 792.0 82 560.0 83 435.5 84 354.5 85 306.0 86 256.5 87 209.5 88 173.5 89 155.0 90 128.0 91 108.5 92 99.5 93 79.0 94 62.0 95 59.5 96 53.5 97 38.5 98 30.0 99 18.5 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01464420415024422 2 0.004974895966363208 3 0.0042958300481059156 4 0.006421601618302656 5 0.002878649001308088 6 0.007233528259697245 7 0.0014762302570810704 8 0.004458215376384834 9 0.010894579297258301 10-11 0.007691159639392377 12-13 0.009388824435035609 14-15 0.00355771491956538 16-17 0.005978732541178336 18-19 0.0037348625504151087 20-21 9.890742722443173E-4 22-23 0.0011957465082356672 24-25 0.007071142931418328 26-27 0.0012031276595210725 28-29 8.931193055340477E-4 30-31 5.757298002616175E-4 32-33 0.0013138449288021528 34-35 0.0016755213417870153 36-37 0.0013655129877999902 38-39 0.0018157632162097168 40-41 0.002878649001308088 42-43 0.0026867390678875485 44-45 0.007824020362529674 46-47 0.014002043988413954 48-49 0.005233236261352395 50-51 0.005963970238607525 52-53 0.013522269154862607 54-55 0.007838782665100484 56-57 0.005617056128193474 58-59 0.006923519905710221 60-61 0.0013655129877999902 62-63 0.0021405338727675523 64-65 0.002295538049761065 66-67 0.0024357799241837663 68-69 0.0022217265369070113 70-71 0.002347206108758902 72-73 0.001380275290370801 74-75 0.0016238532827891777 76-77 0.008185696775514536 78-79 0.006495413131156711 80-81 0.01275462942118045 82-83 0.01550041769935124 84-85 0.0089607176604821 86-87 0.006148499020742659 88-89 0.002967222816732952 90-91 0.0026498333114605217 92-93 9.152627593902638E-4 94-95 0.001380275290370801 96-97 0.001963386241917824 98-99 0.006502794282442116 100-101 0.011787698602792348 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6774011.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.657491371751235 #Duplication Level Percentage of deduplicated Percentage of total 1 70.61752806546042 33.654542344740804 2 15.974685996603414 15.226269200991249 3 5.608511188428491 8.01862720712703 4 2.489227690568121 4.745213887422979 5 1.4172060690423043 3.3770243003688547 6 0.8358792156592961 2.3901543904865457 7 0.5638083356239832 1.8808783623224985 8 0.4035602167913903 1.5386134039774195 9 0.2964690024207178 1.271607203237135 >10 1.590275441021786 13.709066931573464 >50 0.12360069667738012 4.068344420363595 >100 0.07342015309542518 6.39011390195581 >500 0.004370987192900631 1.4084930101753628 >1k 0.001301947647455034 1.0787706738367973 >5k 6.199750679080623E-5 0.19160877007519794 >10k+ 9.299626018620934E-5 1.0506719913452642 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31308 0.4621781688869416 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25504 0.37649776476595626 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14308 0.21121902518315958 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7253 0.10707098054609004 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.952460514162141E-5 0.0 5 0.0 0.0 0.0 2.952460514162141E-5 0.0 6 0.0 0.0 0.0 2.952460514162141E-5 0.0 7 0.0 0.0 0.0 7.381151285405352E-5 0.0 8 0.0 0.0 0.0 7.381151285405352E-5 0.0 9 0.0 0.0 0.0 8.857381542486424E-5 0.0 10-11 0.0 0.0 0.0 1.0333611799567493E-4 0.0 12-13 0.0 0.0 0.0 1.25479571851891E-4 0.0 14-15 0.0 0.0 0.0 1.3286072313729635E-4 0.0 16-17 0.0 0.0 0.0 1.3286072313729635E-4 0.0 18-19 0.0 0.0 0.0 1.4024187442270171E-4 0.0 20-21 0.0 0.0 0.0 1.6238532827891775E-4 0.0 22-23 0.0 0.0 0.0 2.657214462745927E-4 0.0 24-25 0.0 7.381151285405353E-6 0.0 4.7977483355134793E-4 0.0 26-27 0.0 1.4762302570810705E-5 0.0 6.27397859259455E-4 0.0 28-29 0.0 1.4762302570810705E-5 0.0 0.0011071726928108029 0.0 30-31 0.0 1.4762302570810705E-5 0.0 0.002295538049761065 0.0 32-33 0.0 1.4762302570810705E-5 0.0 0.004532026889238886 0.0 34-35 0.0 1.4762302570810705E-5 0.0 0.007971643388237781 0.0 36-37 0.0 1.4762302570810705E-5 0.0 0.013861802113991253 0.0 38-39 0.0 2.214345385621606E-5 0.0 0.022756089412904704 0.0 40-41 0.0 2.952460514162141E-5 0.0 0.034226398510424624 0.0 42-43 0.0 2.952460514162141E-5 0.0 0.046102670928641835 0.0 44-45 0.0 2.952460514162141E-5 0.0 0.060296624850476335 0.0 46-47 0.0 2.952460514162141E-5 1.4762302570810705E-5 0.0776128057660373 0.0 48-49 0.0 2.952460514162141E-5 1.4762302570810705E-5 0.09739429121092363 0.0 50-51 0.0 5.904921028324282E-5 1.4762302570810705E-5 0.1185708142487516 0.0 52-53 0.0 5.904921028324282E-5 1.4762302570810705E-5 0.1397030503788671 0.0 54-55 0.0 8.119266413945887E-5 1.4762302570810705E-5 0.16488753856467017 0.0 56-57 0.0 8.857381542486424E-5 1.4762302570810705E-5 0.1941538034113024 0.0 58-59 0.0 8.857381542486424E-5 1.4762302570810705E-5 0.22888950136042 0.0 60-61 0.0 8.857381542486424E-5 1.4762302570810705E-5 0.2669319550853992 0.0 62-63 0.0 8.857381542486424E-5 1.4762302570810705E-5 0.30669421705987787 0.0 64-65 0.0 8.857381542486424E-5 1.4762302570810705E-5 0.3509885059235953 0.0 66-67 0.0 8.857381542486424E-5 1.4762302570810705E-5 0.40329134393197763 0.0 68-69 0.0 8.857381542486424E-5 1.4762302570810705E-5 0.4632927227310378 0.0 70-71 0.0 8.857381542486424E-5 1.4762302570810705E-5 0.5312066957080525 0.0 72-73 0.0 8.857381542486424E-5 1.4762302570810705E-5 0.6071587424348736 0.0 74-75 0.0 1.0333611799567495E-4 1.4762302570810705E-5 0.6854284706653119 0.0 76-77 0.0 1.0333611799567495E-4 1.4762302570810705E-5 0.7691823943008065 0.0 78-79 0.0 1.0333611799567495E-4 1.4762302570810705E-5 0.8694479533617527 0.0 80-81 0.0 1.0333611799567495E-4 1.4762302570810705E-5 0.9837155564111131 0.0 82-83 0.0 1.0333611799567495E-4 1.4762302570810705E-5 1.1071062624492343 0.0 84-85 0.0 1.0333611799567495E-4 1.4762302570810705E-5 1.2318403380212994 0.0 86-87 0.0 1.0333611799567495E-4 1.4762302570810705E-5 1.3679635300267448 0.0 88-89 0.0 1.0333611799567495E-4 1.4762302570810705E-5 1.5269166229579492 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13500 0.0 59.96243 1 GTATCAA 25960 0.0 44.065144 1 TATAACG 695 0.0 39.638397 2 ATCAACG 32705 0.0 34.6536 3 TCAACGC 33105 0.0 34.222057 4 TATCAAC 34105 0.0 33.20175 2 CAACGCA 34440 0.0 32.86719 5 AACGCAG 35895 0.0 31.720425 6 TACCTGG 5705 0.0 31.637403 2 GTGGTAT 5560 0.0 30.246273 1 GTACCTG 6130 0.0 30.223694 1 ATAACGC 985 0.0 29.89837 3 TAACGCA 1120 0.0 28.840462 4 TGGTATC 5485 0.0 28.576574 2 ACGCAGA 40260 0.0 28.106176 7 CGCAGAG 40545 0.0 27.803164 8 GTACATG 24915 0.0 25.34005 1 GCAGAGT 44800 0.0 24.579283 9 TACATGG 23995 0.0 24.466429 2 ACATGGG 25165 0.0 22.707052 3 >>END_MODULE