FastQCFastQC Report
Wed 25 May 2016
SRR2049401_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049401_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6774011
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316340.4669906795250259No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260750.3849270395338891No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180130.26591335620801326No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74470.10993486724482733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137450.058.3639951
GTATCAA262650.043.0099641
TATAACG7400.041.723822
ATAACGC10000.033.7256853
ATCAACG345500.032.4601363
TCAACGC348350.032.1809354
TATCAAC361200.031.1412732
CAACGCA360700.031.1315365
TACCTGG60350.030.3817252
GTACCTG62650.030.266981
AACGCAG377150.030.0233636
GTGGTAT55800.029.8092541
TAACGCA11950.029.4148224
ACGCAGA434050.025.9443577
CGCAGAG436300.025.7996778
TGGTATC60650.025.7672182
GTACATG252750.023.992571
TACATGG240700.023.6419432
ACCTGGG77000.023.3803443
GCAGAGT482400.023.0786849
Produced by FastQC (version 0.11.5)