##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049401_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6774011 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03978012436059 33.0 31.0 34.0 30.0 34.0 2 32.22334684723718 33.0 31.0 34.0 30.0 34.0 3 32.288711813429295 34.0 31.0 34.0 30.0 34.0 4 35.93906771040083 37.0 35.0 37.0 35.0 37.0 5 35.82752818086655 37.0 35.0 37.0 35.0 37.0 6 35.852718573973384 37.0 35.0 37.0 35.0 37.0 7 35.77986498693315 37.0 35.0 37.0 35.0 37.0 8 35.7719593310374 37.0 35.0 37.0 33.0 37.0 9 37.373764967314045 39.0 37.0 39.0 34.0 39.0 10-11 37.36084086665935 39.0 37.0 39.0 34.0 39.0 12-13 37.33837692321433 39.0 37.0 39.0 34.0 39.0 14-15 38.54518977013766 40.0 38.0 41.0 34.0 41.0 16-17 38.53656880687085 40.0 38.0 41.0 34.0 41.0 18-19 38.57800061145457 40.0 38.0 41.0 34.0 41.0 20-21 38.56104618076351 40.0 38.0 41.0 34.0 41.0 22-23 38.4646976510667 40.0 38.0 41.0 34.0 41.0 24-25 38.363051004788744 40.0 38.0 41.0 34.0 41.0 26-27 38.17659743983292 40.0 38.0 41.0 33.5 41.0 28-29 37.98309162769296 40.0 37.5 41.0 33.0 41.0 30-31 37.77100989354756 40.0 37.0 41.0 33.0 41.0 32-33 37.504523021884665 40.0 36.5 41.0 32.5 41.0 34-35 37.30578736881296 39.0 36.0 41.0 32.0 41.0 36-37 37.093465156758676 39.0 36.0 41.0 31.0 41.0 38-39 37.24229559119405 39.5 36.0 41.0 32.0 41.0 40-41 37.14028003497485 40.0 36.0 41.0 32.0 41.0 42-43 36.9419955178697 40.0 36.0 41.0 31.0 41.0 44-45 36.68711624471823 39.5 36.0 41.0 30.5 41.0 46-47 36.334948599876796 39.0 35.5 41.0 30.0 41.0 48-49 36.0315778938062 39.0 35.0 41.0 29.5 41.0 50-51 35.79246726939179 39.0 35.0 40.0 28.5 41.0 52-53 35.59661395589703 39.0 35.0 40.0 28.0 41.0 54-55 35.482345983790104 39.0 35.0 40.0 28.0 41.0 56-57 35.374174621210386 38.5 35.0 40.0 28.0 41.0 58-59 35.128704839717564 38.0 34.5 40.0 27.0 41.0 60-61 34.86974142793686 38.0 34.0 40.0 26.5 41.0 62-63 34.57561066847987 37.5 34.0 40.0 26.0 41.0 64-65 34.30662977960915 37.0 34.0 40.0 26.0 41.0 66-67 33.96098692488099 36.5 34.0 39.5 26.0 41.0 68-69 33.63452236791467 36.0 33.0 39.0 25.0 41.0 70-71 33.30152792784068 35.5 33.0 39.0 24.5 40.5 72-73 32.935834766137816 35.0 33.0 38.5 24.0 40.0 74-75 32.56687419019544 35.0 32.0 37.5 23.0 40.0 76-77 30.588907295839938 33.5 30.0 35.5 21.0 38.5 78-79 31.908863227414304 35.0 32.0 36.0 23.0 39.0 80-81 31.935127873279214 35.0 32.0 36.0 23.0 39.0 82-83 31.67708068380757 35.0 32.0 36.0 22.0 37.5 84-85 31.425630914977845 35.0 32.0 35.5 21.5 37.0 86-87 31.168704700951917 35.0 32.0 35.0 20.0 37.0 88-89 30.913598235963892 35.0 32.0 35.0 19.5 36.0 90-91 30.650543009156614 34.5 32.0 35.0 18.0 36.0 92-93 30.40922291091644 34.0 31.0 35.0 16.5 36.0 94-95 30.146606124495516 34.0 31.0 35.0 9.0 35.5 96-97 29.903933725528347 34.0 31.0 35.0 2.0 35.0 98-99 29.614030815716127 34.0 31.0 35.0 2.0 35.0 100-101 28.999789710999877 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4131316454579448 1101 2 0.4100220653530542 1101 3 0.39949291290906785 1101 4 0.3013734931858849 1101 5 0.30315513769573244 1101 6 0.3051760829357093 1101 7 0.3385359820977101 1101 8 0.3408491008165413 1101 9 0.41306906480193106 1101 10-11 0.37906515425727605 1101 12-13 0.35291375695940985 1101 14-15 0.47251165405999274 1101 16-17 0.4729117814607662 1101 18-19 0.4306302391667671 1101 20-21 0.3694341950220732 1101 22-23 0.3482956770868739 1101 24-25 0.28272315423571825 1101 26-27 0.2820824179330117 1101 28-29 0.3218427923960263 1101 30-31 0.22675276059658955 1101 32-33 0.28747714728538654 1101 34-35 0.2666793288822831 1101 36-37 0.17894004149666642 1101 38-39 0.35301923238147737 1101 40-41 0.45627143441693363 1101 42-43 0.4859848433924654 1101 44-45 0.42986109840209963 1101 46-47 0.4139328187000402 1101 48-49 0.2932643096636838 1101 50-51 0.3622456070054767 1101 52-53 0.34011068786551135 1101 54-55 0.33876352262128506 1101 56-57 0.20997337354241807 1101 58-59 0.21536866887543482 1101 60-61 0.22252053559282814 1101 62-63 0.17699777822249274 1101 64-65 0.12832225841741973 1101 66-67 0.12554423592404618 1101 68-69 0.07537394147027499 1101 70-71 0.08193276790795778 1101 72-73 0.03898174714367286 1101 74-75 0.05943417842984644 1101 76-77 0.04967562198804387 1101 78-79 0.04325793713170256 1101 80-81 0.028433628469876737 1101 82-83 0.04325611792626205 1101 84-85 0.05140058858942709 1101 86-87 -0.006581001047202051 1101 88-89 -0.0929698424648997 1101 90-91 -0.030142288620172764 1101 92-93 -0.09241854138328165 1101 94-95 -0.09397966055611562 1101 96-97 -0.22344867892571685 1101 98-99 -0.32153864998190684 1101 100-101 -0.23277614131751534 1102 1 0.3983187796770409 1102 2 0.3589786412296334 1102 3 0.3699643870760241 1102 4 0.3057997198828346 1102 5 0.33416192294671276 1102 6 0.321908363848344 1102 7 0.32325917161372075 1102 8 0.34300195525571553 1102 9 0.37441434478149205 1102 10-11 0.3981121096278315 1102 12-13 0.33177840223515176 1102 14-15 0.42073773138671555 1102 16-17 0.38758426752252007 1102 18-19 0.3500117560046263 1102 20-21 0.3573758971485077 1102 22-23 0.3642779796399438 1102 24-25 0.3047730820455712 1102 26-27 0.25110261784749355 1102 28-29 0.35486318499344804 1102 30-31 0.32447328853150026 1102 32-33 0.34016428980586255 1102 34-35 0.30311447639959255 1102 36-37 0.28814363864537285 1102 38-39 0.3453701149499011 1102 40-41 0.30989077396291265 1102 42-43 0.36644131094173815 1102 44-45 0.3976144738676126 1102 46-47 0.3426360442426599 1102 48-49 0.13770381447213964 1102 50-51 0.21912437472249024 1102 52-53 0.3619391394501221 1102 54-55 0.3689973358318994 1102 56-57 0.3178919969273508 1102 58-59 0.2644820452797987 1102 60-61 0.2276746680803754 1102 62-63 0.2888889409208204 1102 64-65 0.20608261113456905 1102 66-67 0.18206503456121226 1102 68-69 0.20929839510652926 1102 70-71 0.15263755174438387 1102 72-73 0.13074825361572806 1102 74-75 0.0788496922549271 1102 76-77 0.15161610442594053 1102 78-79 0.22120487299288527 1102 80-81 0.24267133609587432 1102 82-83 0.27072743939333677 1102 84-85 0.2648550099967757 1102 86-87 0.18277397321374877 1102 88-89 0.1265077747675072 1102 90-91 0.03224672024574815 1102 92-93 0.11467720616857235 1102 94-95 0.07084826597200333 1102 96-97 0.001156563242318498 1102 98-99 0.12260550315718888 1102 100-101 0.0606268647639574 1103 1 0.2924048663247234 1103 2 0.306414081104343 1103 3 0.2433675474956516 1103 4 0.2198384464106482 1103 5 0.21669596276164071 1103 6 0.23875479575639247 1103 7 0.21609004062842985 1103 8 0.2666853394099462 1103 9 0.3596378936433311 1103 10-11 0.31361060600521995 1103 12-13 0.3178485850619097 1103 14-15 0.34395654961780053 1103 16-17 0.38833688167685665 1103 18-19 0.30089752547161197 1103 20-21 0.27047655202806453 1103 22-23 0.3142487213171634 1103 24-25 0.3252818991205615 1103 26-27 0.27134491165138996 1103 28-29 0.34005273222980037 1103 30-31 0.27217522997874966 1103 32-33 0.329291016966927 1103 34-35 0.2780009235556449 1103 36-37 0.27266806027490276 1103 38-39 0.28735672382089206 1103 40-41 0.3302292357337109 1103 42-43 0.36826155877735545 1103 44-45 0.328892176404473 1103 46-47 0.361823419714689 1103 48-49 0.2839121276547516 1103 50-51 0.30952315325044566 1103 52-53 0.2945718512444415 1103 54-55 0.2506223800535494 1103 56-57 0.251179874694742 1103 58-59 0.17815211346879067 1103 60-61 0.2264643447442225 1103 62-63 0.1732120911542765 1103 64-65 0.26715988070726837 1103 66-67 0.21973590629085749 1103 68-69 0.18201856767421987 1103 70-71 0.1646924190412662 1103 72-73 0.11764547661812941 1103 74-75 0.13294239435607125 1103 76-77 0.18935714926139013 1103 78-79 0.22396683783291138 1103 80-81 0.17880951349418694 1103 82-83 0.23184650471013768 1103 84-85 0.1871640731853823 1103 86-87 0.2705484424932898 1103 88-89 0.14315513985200568 1103 90-91 0.19356292989469281 1103 92-93 0.15682618093819656 1103 94-95 0.19341839741660394 1103 96-97 0.17954662294630808 1103 98-99 0.13177494087315722 1103 100-101 0.04014230666618346 1104 1 0.33754031589690214 1104 2 0.3509227044423895 1104 3 0.32217148727073663 1104 4 0.2455223069438759 1104 5 0.26332287992001113 1104 6 0.29110666845142674 1104 7 0.33079390311791457 1104 8 0.3312635955655878 1104 9 0.38004814796946107 1104 10-11 0.3509686746364906 1104 12-13 0.2963635131697657 1104 14-15 0.38959044658960806 1104 16-17 0.40141120260361873 1104 18-19 0.42680246279587664 1104 20-21 0.36592315883167714 1104 22-23 0.3602437321936307 1104 24-25 0.37574003332749584 1104 26-27 0.354266531508145 1104 28-29 0.37038087576335954 1104 30-31 0.35037903529616443 1104 32-33 0.34425847366424733 1104 34-35 0.3629848706046701 1104 36-37 0.38055888987597797 1104 38-39 0.43621538345976063 1104 40-41 0.5063036916692099 1104 42-43 0.456961517723677 1104 44-45 0.5030010843629569 1104 46-47 0.6280327328371271 1104 48-49 0.5612419236675876 1104 50-51 0.6450402470084882 1104 52-53 0.5219111357532924 1104 54-55 0.513910407396942 1104 56-57 0.4662058122132535 1104 58-59 0.5533534211247542 1104 60-61 0.5593368000708026 1104 62-63 0.5577677471244868 1104 64-65 0.5766984711610519 1104 66-67 0.5238526220244708 1104 68-69 0.47717795374269656 1104 70-71 0.4005240796899443 1104 72-73 0.34859684730111695 1104 74-75 0.3873192429768437 1104 76-77 0.4137040273223356 1104 78-79 0.5605088097042525 1104 80-81 0.5344361916319968 1104 82-83 0.4906799328710285 1104 84-85 0.4524386575169288 1104 86-87 0.38139775415542587 1104 88-89 0.4441347969298377 1104 90-91 0.36465911121416994 1104 92-93 0.29894318666504915 1104 94-95 0.2817180658186338 1104 96-97 0.25659639639339815 1104 98-99 0.25494896505625064 1104 100-101 0.2535588161670752 1105 1 0.30521459315308874 1105 2 0.29063566798517826 1105 3 0.250517674907762 1105 4 0.18728774615139798 1105 5 0.20620591127181598 1105 6 0.18603565455411797 1105 7 0.20138619810453662 1105 8 0.19732748287197666 1105 9 0.24006536199408401 1105 10-11 0.2548955248323068 1105 12-13 0.2678546357325686 1105 14-15 0.30435031871012086 1105 16-17 0.30673965683393334 1105 18-19 0.3308955178062476 1105 20-21 0.2601418765612138 1105 22-23 0.2023840857637751 1105 24-25 0.2231431610581609 1105 26-27 0.24627292208856488 1105 28-29 0.2625294579037103 1105 30-31 0.27505936202576464 1105 32-33 0.3427327516501748 1105 34-35 0.3286289353681653 1105 36-37 0.29784702678512787 1105 38-39 0.34688436855100946 1105 40-41 0.3450234504539438 1105 42-43 0.4229930825953616 1105 44-45 0.40985746677208823 1105 46-47 0.40523425614296826 1105 48-49 0.3943335440769147 1105 50-51 0.3872068772734423 1105 52-53 0.41479958917920356 1105 54-55 0.4125060817188384 1105 56-57 0.40821399318121365 1105 58-59 0.40443361029149827 1105 60-61 0.44983303906904126 1105 62-63 0.4796037441471839 1105 64-65 0.49204258449410787 1105 66-67 0.4674114822450903 1105 68-69 0.42334924181537303 1105 70-71 0.33751021301119266 1105 72-73 0.463777833843686 1105 74-75 0.4605783902025351 1105 76-77 0.5111389535235809 1105 78-79 0.5378031743618976 1105 80-81 0.4129785815600755 1105 82-83 0.49816570438126107 1105 84-85 0.5084617608983173 1105 86-87 0.49080123012070587 1105 88-89 0.5027969506319785 1105 90-91 0.5252053041459881 1105 92-93 0.49274240216983856 1105 94-95 0.5280846079557868 1105 96-97 0.4757150221258648 1105 98-99 0.4644060211262655 1105 100-101 0.4957937420798224 1106 1 0.26179778807608045 1106 2 0.20648995366450862 1106 3 0.2227106705361237 1106 4 0.20479139633270194 1106 5 0.22560569285360543 1106 6 0.2692009653455827 1106 7 0.2618278929144111 1106 8 0.23322557896724305 1106 9 0.25604920816566334 1106 10-11 0.24190663068442575 1106 12-13 0.27985911185434986 1106 14-15 0.2922163433699936 1106 16-17 0.312916763210346 1106 18-19 0.2996664295054714 1106 20-21 0.2676911062838485 1106 22-23 0.29658665360079794 1106 24-25 0.3194810186661101 1106 26-27 0.35720404640061076 1106 28-29 0.4379106359688194 1106 30-31 0.3550930166097075 1106 32-33 0.33588136826193704 1106 34-35 0.3414097412386923 1106 36-37 0.4039467569031814 1106 38-39 0.4460758784563268 1106 40-41 0.3876639469111396 1106 42-43 0.3564159370966209 1106 44-45 0.37588134275997476 1106 46-47 0.4109914672728152 1106 48-49 0.3817639852589281 1106 50-51 0.4248070038441938 1106 52-53 0.4301010342649718 1106 54-55 0.41399902029274216 1106 56-57 0.39865954338713294 1106 58-59 0.4169645771169428 1106 60-61 0.40829555732786815 1106 62-63 0.3804859108244045 1106 64-65 0.3926825819282129 1106 66-67 0.4045667307212213 1106 68-69 0.39399306421985614 1106 70-71 0.40686637285840277 1106 72-73 0.4511161951442162 1106 74-75 0.4187048316842379 1106 76-77 0.341842717437693 1106 78-79 0.36682962864985313 1106 80-81 0.45764972675722504 1106 82-83 0.40634365925328453 1106 84-85 0.364899136616625 1106 86-87 0.3946014183837079 1106 88-89 0.4023215412319985 1106 90-91 0.3729625135432677 1106 92-93 0.3896852064281866 1106 94-95 0.4087551666983238 1106 96-97 0.4289517218705079 1106 98-99 0.5471077737351777 1106 100-101 0.6057849353300746 1107 1 0.18547575974499608 1107 2 0.1775841456519558 1107 3 0.16172319157070802 1107 4 0.17992356659865294 1107 5 0.16515956840498092 1107 6 0.15309395176053897 1107 7 0.16341051706791632 1107 8 0.1222425196836312 1107 9 0.1921942166312789 1107 10-11 0.17410437633027698 1107 12-13 0.17679233759209012 1107 14-15 0.18787052223359524 1107 16-17 0.1612116783242996 1107 18-19 0.1092612155713013 1107 20-21 0.13978153893227585 1107 22-23 0.15583675996287383 1107 24-25 0.15024086162213734 1107 26-27 0.188942907436072 1107 28-29 0.23831062436381245 1107 30-31 0.2758709290988648 1107 32-33 0.2968930455502914 1107 34-35 0.3266613493474182 1107 36-37 0.20625182129965225 1107 38-39 0.23068466840004476 1107 40-41 0.2746170030687125 1107 42-43 0.23321785123183503 1107 44-45 0.1968592854040523 1107 46-47 0.19066283978764176 1107 48-49 0.16777843332409503 1107 50-51 0.19830351993908124 1107 52-53 0.22149490903391467 1107 54-55 0.25157320714833986 1107 56-57 0.30612170045239395 1107 58-59 0.31775846990981194 1107 60-61 0.27048160838470636 1107 62-63 0.2787383016544638 1107 64-65 0.31865523348784563 1107 66-67 0.3435301647272766 1107 68-69 0.3227615086862414 1107 70-71 0.3227427945278549 1107 72-73 0.3727997725559007 1107 74-75 0.3791037327265556 1107 76-77 0.3551053586009125 1107 78-79 0.4033390358737421 1107 80-81 0.47554655120659817 1107 82-83 0.4908399312075069 1107 84-85 0.4662855893611031 1107 86-87 0.4649235283278834 1107 88-89 0.49209704194332105 1107 90-91 0.5248258213942201 1107 92-93 0.4694703667434652 1107 94-95 0.4999550747623722 1107 96-97 0.5513917429255351 1107 98-99 0.6467981761547037 1107 100-101 0.6983757076015635 1108 1 0.0987327062636254 1108 2 0.14388713866324565 1108 3 0.07853951497988021 1108 4 0.08552676420075755 1108 5 0.03327186451404884 1108 6 0.05579856159919672 1108 7 0.09621545580870361 1108 8 0.07284912258059961 1108 9 0.14595390211508175 1108 10-11 0.06294203243393781 1108 12-13 0.015421159672214912 1108 14-15 0.038652949936675896 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-0.9266179124432412 2316 100-101 -1.2162826535131366 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 16.0 4 60.0 5 165.0 6 363.0 7 956.0 8 2586.0 9 5177.0 10 8392.0 11 12833.0 12 18963.0 13 26858.0 14 30903.0 15 37977.0 16 47689.0 17 63772.0 18 54066.0 19 31933.0 20 25657.0 21 25147.0 22 25990.0 23 28518.0 24 33491.0 25 42293.0 26 53253.0 27 67313.0 28 85817.0 29 107730.0 30 136680.0 31 173864.0 32 222711.0 33 293743.0 34 411304.0 35 617991.0 36 946337.0 37 1426444.0 38 1399538.0 39 307385.0 40 88.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.52433982858214 21.55244963880548 16.029976708086004 22.89323382452637 2 14.76475627512697 21.63833933656901 39.173530196356666 24.423374191947353 3 16.62811732266982 27.162451383982468 31.3132177994633 24.89621349388441 4 12.13275760259817 18.74130498966637 38.01039267788603 31.115544729849425 5 12.581750219779488 37.65819509923109 34.989662429037615 14.770392251951812 6 29.20691196591139 38.52181527976007 18.1731256964255 14.098147057903038 7 26.296296241621103 33.292003806902585 22.561330355087996 17.850369596388312 8 25.99082375136736 34.89236065491489 20.213093869067215 18.90372172465054 9 25.38724909761575 16.712653781023587 21.695353069877925 36.204744051482734 10-11 23.903046187272558 27.21093963707459 28.347467504102813 20.538546671550037 12-13 24.917962194038363 24.84849817929141 28.777211905915124 21.4563277207551 14-15 22.07004184689678 26.35563972076087 27.18036179326803 24.393956639074315 16-17 20.799501211320738 29.684680169548 27.578254596870305 21.93756402226096 18-19 20.93237583991099 27.655383855920423 30.92814422306799 20.4840960811006 20-21 21.817000424566267 26.7919988449908 30.97554876223285 20.415451968210085 22-23 21.4141518223103 26.803226330751457 31.003994531452634 20.77862731548561 24-25 21.13601244176687 26.954605192016633 30.888797279876645 21.020585086339853 26-27 21.044914940200517 26.974925702778634 30.863176481183203 21.116982875837643 28-29 21.09619399292548 26.894874387379513 31.05099776502819 20.95793385466682 30-31 21.29792625012059 27.189876964450434 30.703410048204617 20.80878673722436 32-33 20.882874587465324 27.07475719978153 30.9551095394028 21.08725867335034 34-35 21.222103186698185 26.96337441867452 30.747407347970295 21.067115046656994 36-37 21.646063396539724 26.987929164669964 30.334772137221677 21.03123530156863 38-39 21.373660506289795 27.319094904683144 30.374080948025252 20.93316364100181 40-41 21.12666439864122 27.104648509816492 30.581651093490652 21.187035998051634 42-43 21.209640441682087 27.284230612634747 30.288487230883376 21.217641714799786 44-45 21.2317413705687 27.464061059164184 29.953603910389422 21.3505936598777 46-47 21.60780682178686 27.652265516853998 29.404737810440047 21.335189850919097 48-49 21.608134155189145 27.607619086704894 29.320033573194237 21.464213184911728 50-51 21.61619619624496 27.792059285096947 28.90941961671628 21.68232490194181 52-53 21.589072821383134 27.776621371973626 28.94185768768245 21.692448118960794 54-55 21.698994982774856 28.18200157116288 28.72975326778776 21.38925017827451 56-57 21.55860338819314 27.85726559545225 28.838392829990696 21.74573818636391 58-59 21.542823606853688 27.582927348293424 29.018838547309834 21.855410497543055 60-61 21.75001253028028 27.782410557235405 28.91362728325143 21.553949629232882 62-63 21.5461962763787 27.79583089995093 29.04383867575182 21.614134147918552 64-65 21.666601834870242 27.870702122450314 28.8479842490474 21.61471179363205 66-67 21.568879291897332 27.79868846200435 28.80040828796821 21.832023958130108 68-69 21.68460228603115 28.056380422134186 28.647029018735697 21.611988273098962 70-71 21.867725918687288 27.707481572665653 28.693326152764392 21.731466355882667 72-73 21.502890548869917 27.988840522393577 28.644408532481602 21.8638603962549 74-75 21.54851195212595 27.916874699392096 28.621120220426604 21.91349312805535 76-77 21.588346777610372 28.269246042817965 28.359866109349934 21.782541070221733 78-79 21.623409302585817 28.16529981456595 28.52320627142342 21.68808461142481 80-81 21.537930544776923 28.168784019125038 28.488811618986688 21.804473817111354 82-83 21.425136023787424 28.149952487445006 28.61597706170314 21.80893442706443 84-85 21.372150637764666 28.120318477844314 28.44215328908947 22.065377595301545 86-87 21.511457831929164 28.189749878408144 28.425699716941388 21.873092572721305 88-89 21.289348051651455 28.21342560220578 28.443642599939633 22.053583746203127 90-91 21.316186701210892 28.03583594444875 28.61362504013552 22.034352314204835 92-93 21.3860485783521 28.220424992305144 28.38241926305044 22.01110716629232 94-95 21.443062801622855 27.959814177217208 28.48747287599419 22.109650145165745 96-97 21.37522712807296 28.159858305002526 28.383559666084366 22.081354900840147 98-99 21.120387471510423 28.116861768927492 28.457223527299874 22.30552723226221 100-101 21.11044852971515 28.229650662631833 28.23240383226451 22.427496975388507 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3689.0 1 2711.0 2 4160.0 3 5876.0 4 3675.0 5 2311.5 6 3827.0 7 8247.5 8 13252.5 9 14872.0 10 11848.5 11 8589.0 12 8194.5 13 8832.0 14 9679.5 15 10286.5 16 10793.5 17 11531.5 18 12397.5 19 13299.0 20 14749.0 21 16827.0 22 20374.5 23 26506.0 24 34375.0 25 43666.0 26 56271.0 27 71048.5 28 87840.0 29 105240.0 30 118334.0 31 131846.0 32 146951.5 33 159863.0 34 170416.5 35 181888.0 36 192231.5 37 200889.5 38 212524.5 39 219512.0 40 225314.0 41 231003.0 42 232377.0 43 232568.5 44 230655.0 45 226676.0 46 222246.5 47 216044.0 48 208122.0 49 204866.0 50 202679.0 51 194961.5 52 181063.5 53 168874.5 54 162519.0 55 157317.5 56 148514.0 57 134424.5 58 122376.0 59 113819.5 60 103465.5 61 90652.0 62 77058.5 63 63898.5 64 52308.0 65 43708.5 66 35943.0 67 28223.5 68 21588.0 69 16011.0 70 11980.0 71 8737.5 72 6516.5 73 4997.0 74 3894.5 75 2948.0 76 2118.5 77 1639.0 78 1332.0 79 1089.5 80 721.0 81 378.0 82 251.5 83 169.0 84 103.5 85 77.5 86 63.5 87 39.0 88 29.5 89 25.0 90 15.5 91 7.5 92 4.5 93 4.0 94 3.0 95 1.5 96 2.5 97 2.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05050183709474342 2 0.0024210176216129557 3 1.3286072313729635E-4 4 1.6238532827891775E-4 5 0.002598165252462684 6 0.0019929108470594452 7 0.0 8 1.4762302570810705E-5 9 1.7714763084972847E-4 10-11 2.952460514162141E-5 12-13 0.0 14-15 4.7239368226594257E-4 16-17 0.0 18-19 6.643036156864818E-5 20-21 5.757298002616175E-4 22-23 0.0 24-25 7.897831875383727E-4 26-27 0.007986405690808591 28-29 0.008082360657518863 30-31 0.0010997915415253976 32-33 0.00722614710841184 34-35 0.008702377365492911 36-37 0.0048567975457967216 38-39 0.002701501370458359 40-41 0.0019264804854907972 42-43 0.0013138449288021528 44-45 8.340700952508049E-4 46-47 5.609674976908069E-4 48-49 0.002280775747190254 50-51 0.0014688491057956653 52-53 0.002199583083050795 54-55 7.012093721135086E-4 56-57 2.50959143703782E-4 58-59 8.045454901091835E-4 60-61 0.006192785928455091 62-63 0.0025834029498918738 64-65 0.0010555046338129655 66-67 0.00532181007677726 68-69 0.00775020884967562 70-71 0.0025760217986064682 72-73 5.609674976908069E-4 74-75 0.003498665709282137 76-77 0.0018452878213513382 78-79 0.0025686406473210627 80-81 9.447873645318851E-4 82-83 1.7714763084972847E-4 84-85 5.757298002616175E-4 86-87 0.004805129486798885 88-89 0.0027236448243145753 90-91 0.0012695580210897207 92-93 8.857381542486424E-5 94-95 4.4286907712432123E-4 96-97 3.616764129848623E-4 98-99 6.200167079740496E-4 100-101 7.381151285405353E-6 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6774011.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.664033674744964 #Duplication Level Percentage of deduplicated Percentage of total 1 67.97477654874501 31.040024853551568 2 17.3698197423459 15.863520672774664 3 6.11361930218373 8.37517553068466 4 2.80094832798064 5.116103950805142 5 1.5319157297872323 3.4976725735937837 6 0.9119794467540213 2.498679610035055 7 0.598328450662822 1.912546336373756 8 0.4180432424866322 1.5271632561927322 9 0.3218466830643833 1.322713599620127 >10 1.7433408463145232 14.323563140592318 >50 0.13151662390890195 4.128493381412935 >100 0.07749365989912409 6.4761411399043025 >500 0.004876111019643394 1.5166793555293945 >1k 0.0013327538861368873 1.0803942353776257 >5k 6.501238447165667E-5 0.1971383638341716 >10k+ 9.751857670748499E-5 1.1239899997177278 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31634 0.4669906795250259 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26075 0.3849270395338891 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18013 0.26591335620801326 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7447 0.10993486724482733 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 7.381151285405353E-6 0.0 0.0 0.0 14-15 0.0 2.214345385621606E-5 0.0 0.0 0.0 16-17 0.0 2.952460514162141E-5 0.0 7.381151285405353E-6 0.0 18-19 0.0 3.690575642702676E-5 0.0 1.4762302570810705E-5 0.0 20-21 0.0 4.428690771243212E-5 0.0 2.952460514162141E-5 0.0 22-23 0.0 4.428690771243212E-5 0.0 1.4762302570810708E-4 0.0 24-25 0.0 5.904921028324282E-5 0.0 3.838198668410784E-4 0.0 26-27 0.0 5.904921028324282E-5 0.0 5.609674976908069E-4 0.0 28-29 0.0 5.904921028324282E-5 0.0 0.0010555046338129653 0.0 30-31 0.0 5.904921028324282E-5 0.0 0.002236488839477822 0.0 32-33 0.0 5.904921028324282E-5 0.0 0.00449512113281186 0.0 34-35 0.0 5.904921028324282E-5 0.0 0.007964262236952376 0.0 36-37 0.0 5.904921028324282E-5 0.0 0.013861802113991253 0.0 38-39 0.0 5.904921028324282E-5 0.0 0.022741327110333893 0.0 40-41 0.0 5.904921028324282E-5 0.0 0.034086156636001924 0.0 42-43 0.0 7.381151285405352E-5 0.0 0.04576313796951319 0.0 44-45 0.0 7.381151285405352E-5 0.0 0.05977994426049796 0.0 46-47 0.0 7.381151285405352E-5 0.0 0.0769411209990654 0.0 48-49 0.0 8.857381542486424E-5 0.0 0.09664879493109768 0.0 50-51 0.0 8.857381542486424E-5 0.0 0.11767031379193213 0.0 52-53 0.0 8.857381542486424E-5 0.0 0.1385811153834855 0.0 54-55 0.0 8.857381542486424E-5 0.0 0.16345559521530154 0.0 56-57 0.0 8.857381542486424E-5 0.0 0.19226422868223864 0.0 58-59 0.0 8.857381542486424E-5 0.0 0.22671944288251084 0.0 60-61 0.0 8.857381542486424E-5 0.0 0.26441498249707596 0.0 62-63 0.0 8.857381542486424E-5 0.0 0.30373437539443027 0.0 64-65 0.0 1.0333611799567495E-4 0.0 0.3475857951810235 0.0 66-67 0.0 1.1809842056648566E-4 0.0 0.3994310018097107 0.0 68-69 0.0 1.3286072313729635E-4 0.0 0.45852449900066594 0.0 70-71 0.0 1.3286072313729635E-4 0.0 0.5257741683619941 0.0 72-73 0.0 1.3286072313729635E-4 0.0 0.6012095344988369 0.0 74-75 0.0 1.3286072313729635E-4 0.0 0.6785639999698849 0.0 76-77 0.0 1.3286072313729635E-4 0.0 0.7616979068974055 0.0 78-79 0.0 1.3286072313729635E-4 0.0 0.8613803550068047 0.0 80-81 0.0 1.3286072313729635E-4 0.0 0.974791744507058 0.0 82-83 0.0 1.3286072313729635E-4 0.0 1.097156470516508 0.0 84-85 0.0 1.3286072313729635E-4 0.0 1.2210048079343243 0.0 86-87 0.0 1.3286072313729635E-4 0.0 1.356168450272667 0.0 88-89 0.0 1.3286072313729635E-4 0.0 1.5141988992931958 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13745 0.0 58.363995 1 GTATCAA 26265 0.0 43.009964 1 TATAACG 740 0.0 41.72382 2 ATAACGC 1000 0.0 33.725685 3 ATCAACG 34550 0.0 32.460136 3 TCAACGC 34835 0.0 32.180935 4 TATCAAC 36120 0.0 31.141273 2 CAACGCA 36070 0.0 31.131536 5 TACCTGG 6035 0.0 30.381725 2 GTACCTG 6265 0.0 30.26698 1 AACGCAG 37715 0.0 30.023363 6 GTGGTAT 5580 0.0 29.809254 1 TAACGCA 1195 0.0 29.414822 4 ACGCAGA 43405 0.0 25.944357 7 CGCAGAG 43630 0.0 25.799677 8 TGGTATC 6065 0.0 25.767218 2 GTACATG 25275 0.0 23.99257 1 TACATGG 24070 0.0 23.641943 2 ACCTGGG 7700 0.0 23.380344 3 GCAGAGT 48240 0.0 23.078684 9 >>END_MODULE