FastQCFastQC Report
Wed 25 May 2016
SRR2049400_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049400_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7208630
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319870.443732026751269No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261830.3632174213408096No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148930.20659958965850655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148800.054.3379671
GTATCAA281850.042.1880651
ATCAACG342100.034.1730883
TCAACGC344400.033.9733964
TATCAAC355250.033.0536162
CAACGCA359650.032.5451555
AACGCAG372750.031.5160776
TATAACG7350.031.0211932
ACGCAGA416350.027.908837
CGCAGAG420000.027.4740058
GCAGAGT447500.025.0637869
GTGGTAT66100.024.9387551
TGGTATC70850.022.7952142
GTACATG245500.021.885571
AGAGTAC406850.021.84573410-11
GAGTACT301450.021.69960612-13
CAGAGTA433850.021.60850310-11
ATAACGC10000.021.3765773
TACATGG244050.020.845642
TACCTGG50550.020.6731222
Produced by FastQC (version 0.11.5)