FastQCFastQC Report
Wed 25 May 2016
SRR2049400_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049400_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7208630
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318510.4418453991951314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259800.36040135226804537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180200.24997815118822855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145900.054.325411
GTATCAA269200.040.828441
ATCAACG343700.031.6186163
TCAACGC346900.031.2995664
TATCAAC358000.030.542232
CAACGCA358600.030.3710775
TATAACG7750.029.4183272
AACGCAG372950.029.4056426
ACGCAGA428250.025.4752227
CGCAGAG433050.025.1489818
GTGGTAT64350.024.516121
GCAGAGT468100.023.022869
ATAACGC10200.021.8858853
AGAGTAC426650.021.6526610-11
GTACATG231700.021.2469221
TGGTATC70250.021.1629622
GAGTACT315850.021.14320612-13
AGTACTT324050.020.6155112-13
CAGAGTA451200.020.25346610-11
TACATGG230500.020.0090622
Produced by FastQC (version 0.11.5)