FastQCFastQC Report
Wed 25 May 2016
SRR2049399_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049399_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9401040
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT366390.38973347629623956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301640.32085811782526186No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165170.1756933275467395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA176050.056.4787981
GTATCAA343550.043.71061
ATCAACG419250.035.30493
TCAACGC420950.035.0961154
TATCAAC436700.034.0239682
CAACGCA436900.033.8025
AACGCAG455850.032.5428856
ACGCAGA503900.029.1867497
CGCAGAG510700.028.7423158
GCAGAGT553800.025.6562169
GTGGTAT75800.025.5080621
GTACATG310000.023.400741
TGGTATC81750.022.8352742
GAGTACT367900.022.660212-13
TACATGG306550.022.5301722
CAGAGTA536600.022.00305710-11
AGAGTAC508550.022.00236310-11
ACATGGG312100.020.9580483
GTACTTT397750.020.88573814-15
AGTACTT380350.020.30111912-13
Produced by FastQC (version 0.11.5)