##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049399_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9401040 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.35601752572056 31.0 31.0 34.0 30.0 34.0 2 31.398365074502397 31.0 31.0 34.0 28.0 34.0 3 31.41850167641027 33.0 31.0 34.0 28.0 34.0 4 35.035729557580865 37.0 35.0 37.0 32.0 37.0 5 35.055586722320086 37.0 35.0 37.0 32.0 37.0 6 35.14338158331419 37.0 35.0 37.0 32.0 37.0 7 35.08657063473829 37.0 35.0 37.0 32.0 37.0 8 35.053663743585815 37.0 35.0 37.0 32.0 37.0 9 36.62095417102788 39.0 37.0 39.0 32.0 39.0 10-11 36.55117109383643 39.0 37.0 39.0 32.0 39.0 12-13 36.510416773037875 39.0 37.0 39.0 32.0 39.0 14-15 37.65842220647929 40.0 38.0 41.0 32.0 41.0 16-17 37.64675121050437 40.0 38.0 41.0 32.0 41.0 18-19 37.67374439423723 40.0 38.0 41.0 32.0 41.0 20-21 37.63615972275407 40.0 38.0 41.0 32.0 41.0 22-23 37.53196821840988 40.0 37.5 41.0 32.0 41.0 24-25 37.4195588998664 40.0 37.0 41.0 32.0 41.0 26-27 37.274425542280426 40.0 37.0 41.0 32.0 41.0 28-29 37.11266870473905 40.0 37.0 41.0 31.0 41.0 30-31 36.920906091240965 40.0 36.5 41.0 31.0 41.0 32-33 36.665776073710994 39.5 36.0 41.0 30.0 41.0 34-35 36.464609553836596 39.0 36.0 41.0 30.0 41.0 36-37 36.270492839090146 39.0 35.0 41.0 30.0 41.0 38-39 36.062214818786 39.0 35.0 40.5 29.5 41.0 40-41 35.84420064163114 39.0 35.0 40.0 29.0 41.0 42-43 35.603561839966645 39.0 35.0 40.0 27.5 41.0 44-45 35.314247732165796 38.5 35.0 40.0 26.5 41.0 46-47 34.95331468646022 38.0 34.5 40.0 25.5 41.0 48-49 34.647071281475235 38.0 34.0 40.0 24.0 41.0 50-51 33.286031439074826 36.5 32.5 39.0 23.0 40.0 52-53 33.58740522325189 37.5 33.5 39.0 23.5 40.0 54-55 34.63455532579374 38.5 34.5 40.0 24.5 41.0 56-57 34.749099195408164 39.0 34.5 40.0 24.5 41.0 58-59 34.59856026567273 38.5 34.0 40.0 24.0 41.0 60-61 34.34084728923608 38.0 34.0 40.0 23.0 41.0 62-63 34.07217908869657 38.0 34.0 40.0 23.0 41.0 64-65 33.79620946193187 37.0 34.0 40.0 22.0 41.0 66-67 33.474564622637494 37.0 33.0 40.0 21.5 41.0 68-69 33.134932464918776 36.0 33.0 39.5 20.0 41.0 70-71 32.80212125466969 36.0 33.0 39.0 19.5 41.0 72-73 32.546869867589116 35.0 32.5 39.0 18.5 40.0 74-75 32.350009679780115 35.0 33.0 38.5 18.0 40.0 76-77 32.008735469692716 35.0 32.0 37.5 17.0 40.0 78-79 31.634618137993243 35.0 32.0 37.0 13.0 39.0 80-81 31.29312326083072 35.0 32.0 36.5 9.5 39.0 82-83 30.945696380400467 35.0 31.5 36.0 7.0 38.5 84-85 30.621615534026024 35.0 31.0 36.0 4.0 37.0 86-87 30.32192746759933 35.0 31.0 35.0 2.0 37.0 88-89 30.026500365916963 35.0 31.0 35.0 2.0 36.5 90-91 29.753410101435584 34.0 31.0 35.0 2.0 36.0 92-93 29.49177282513424 34.0 31.0 35.0 2.0 36.0 94-95 29.223138344268293 34.0 30.0 35.0 2.0 36.0 96-97 28.956218248193814 34.0 30.0 35.0 2.0 35.0 98-99 28.627823091913235 34.0 30.0 35.0 2.0 35.0 100-101 28.076577910529046 34.0 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28748280436510854 1101 2 0.3095914769593193 1101 3 0.3154916905543601 1101 4 0.2911396131658748 1101 5 0.33116248357892175 1101 6 0.30787615956491265 1101 7 0.3413631857697794 1101 8 0.3161043222076785 1101 9 0.36370050349938055 1101 10-11 0.3423199089517297 1101 12-13 0.3303312574842394 1101 14-15 0.3866614364043599 1101 16-17 0.4162238383585475 1101 18-19 0.37356395061326 1101 20-21 0.3044623124463186 1101 22-23 0.3528112756284685 1101 24-25 0.34197318737061266 1101 26-27 0.3252573558594136 1101 28-29 0.30582987619563795 1101 30-31 0.2880166918652094 1101 32-33 0.25213117100354765 1101 34-35 0.28548720839262387 1101 36-37 0.2786280347442016 1101 38-39 0.20895721159296698 1101 40-41 0.20979334903093871 1101 42-43 0.12064346402872417 1101 44-45 0.054802641132035035 1101 46-47 -0.02068452933418996 1101 48-49 -0.058892875431567404 1101 50-51 -0.1206093981072911 1101 52-53 0.005668979581450628 1101 54-55 0.2730870439018531 1101 56-57 0.2628233668225235 1101 58-59 0.20851095443754986 1101 60-61 0.26766788180668044 1101 62-63 0.10418647671012593 1101 64-65 0.1359652501953974 1101 66-67 0.12207753578957892 1101 68-69 0.0634386424106097 1101 70-71 -0.015049284467295365 1101 72-73 -0.03902171351900563 1101 74-75 -0.08835069518507765 1101 76-77 -0.06402384053101429 1101 78-79 -0.12656148476226647 1101 80-81 -0.2078087408395497 1101 82-83 -0.15281190632313724 1101 84-85 -0.23522479556290676 1101 86-87 -0.2874565052654994 1101 88-89 -0.32432005686384 1101 90-91 -0.3901982728224809 1101 92-93 -0.4283556112616118 1101 94-95 -0.5629510074842905 1101 96-97 -0.6650172367650278 1101 98-99 -0.8250315351214219 1101 100-101 -1.0407087474448566 1102 1 0.3380252438929503 1102 2 0.32745130664903854 1102 3 0.35310231800678693 1102 4 0.36676916352242017 1102 5 0.3565258189220657 1102 6 0.4172308956070694 1102 7 0.39870922249741625 1102 8 0.4769765365567835 1102 9 0.4686434579572065 1102 10-11 0.4809375650783565 1102 12-13 0.4190732833201807 1102 14-15 0.5282647326509675 1102 16-17 0.44742030676353295 1102 18-19 0.4630770537473836 1102 20-21 0.4354977686156829 1102 22-23 0.4420936969541316 1102 24-25 0.41455863415905014 1102 26-27 0.4939044405477162 1102 28-29 0.47110535724359437 1102 30-31 0.3666320511262171 1102 32-33 0.4170728894934541 1102 34-35 0.37659257182993855 1102 36-37 0.280295235351808 1102 38-39 0.24571391767347706 1102 40-41 0.31123737233679094 1102 42-43 0.2543748912476076 1102 44-45 0.24553164663135618 1102 46-47 0.19953570343519544 1102 48-49 0.13294979909780125 1102 50-51 0.16599769509146967 1102 52-53 0.31910888645526114 1102 54-55 0.5002773128183478 1102 56-57 0.5002102547463068 1102 58-59 0.4707152472482008 1102 60-61 0.4884097167881336 1102 62-63 0.48897218410643006 1102 64-65 0.420539206416521 1102 66-67 0.3747621167975055 1102 68-69 0.3744392644919614 1102 70-71 0.3730087638408648 1102 72-73 0.3449621046832192 1102 74-75 0.2859357335495005 1102 76-77 0.2853699403642551 1102 78-79 0.2861441014146706 1102 80-81 0.24514100522596038 1102 82-83 0.193911308495327 1102 84-85 0.14950300712008513 1102 86-87 0.12768514133851383 1102 88-89 0.08551919071221903 1102 90-91 0.04115248554010975 1102 92-93 -0.0518806765352231 1102 94-95 -0.14059327771781582 1102 96-97 -0.17676826179311078 1102 98-99 -0.2479813724469544 1102 100-101 -0.3443210854638572 1103 1 0.1997417222374196 1103 2 0.1586941484005564 1103 3 0.16709993568369086 1103 4 0.160593259428353 1103 5 0.14179224532644952 1103 6 0.19855230695197434 1103 7 0.19860360792102227 1103 8 0.17976654485347865 1103 9 0.26796219988226255 1103 10-11 0.25801679837386615 1103 12-13 0.20889942303379883 1103 14-15 0.26406140412432677 1103 16-17 0.3008802995124995 1103 18-19 0.22264907881179852 1103 20-21 0.25406650985757295 1103 22-23 0.19464328960074795 1103 24-25 0.26236402538778236 1103 26-27 0.26645607767124346 1103 28-29 0.2481724999064241 1103 30-31 0.2050462720748243 1103 32-33 0.13106697144105794 1103 34-35 0.17659254952248915 1103 36-37 0.19429358917424366 1103 38-39 0.11269151102077757 1103 40-41 0.03964496630256065 1103 42-43 -0.001019708689916854 1103 44-45 -0.04949386109016274 1103 46-47 -0.11192761613389024 1103 48-49 -0.06257145802462105 1103 50-51 -0.10712582672690729 1103 52-53 0.009915761630686859 1103 54-55 0.12529288759436952 1103 56-57 0.2001145446734327 1103 58-59 0.2156559225624548 1103 60-61 0.13070925808581535 1103 62-63 0.07698443892186901 1103 64-65 0.10891578077109898 1103 66-67 0.19663361958339465 1103 68-69 0.10999387235309399 1103 70-71 0.11316616625543219 1103 72-73 0.10840110222699906 1103 74-75 0.0664272467674678 1103 76-77 0.008857422014294514 1103 78-79 -0.029588321284332864 1103 80-81 -0.0194000189097423 1103 82-83 -0.03676891730281184 1103 84-85 -0.029468956721562023 1103 86-87 -0.09545557672795368 1103 88-89 -0.1588607257430681 1103 90-91 -0.17288166614725142 1103 92-93 -0.18928714771174882 1103 94-95 -0.1768622032805105 1103 96-97 -0.28624401631021 1103 98-99 -0.2961853753275143 1103 100-101 -0.3228012204372561 1104 1 -0.04315448816385725 1104 2 -0.023253962687398655 1104 3 0.04687102577144486 1104 4 -0.02886405846797402 1104 5 0.03826798669734899 1104 6 -0.054674203946269984 1104 7 -0.04446998876805708 1104 8 -0.0558800484288966 1104 9 -0.025297203329799345 1104 10-11 -0.0023980193780133163 1104 12-13 -0.08568421618911515 1104 14-15 -0.05484472106964233 1104 16-17 0.008379538953043664 1104 18-19 -0.10503876294363579 1104 20-21 -0.08567382378090116 1104 22-23 -0.13827279255555425 1104 24-25 -0.09930769387637639 1104 26-27 -0.09246106091651285 1104 28-29 -0.05814588027581635 1104 30-31 -0.07590504746501381 1104 32-33 -0.14825117845117575 1104 34-35 -0.17788787815425877 1104 36-37 -0.16686845256985094 1104 38-39 -0.1937571568980232 1104 40-41 -0.2088551502115905 1104 42-43 -0.17386783201802558 1104 44-45 -0.2529944882949948 1104 46-47 -0.20350291754408545 1104 48-49 -0.2975335067239726 1104 50-51 -0.2985783433092948 1104 52-53 -0.2656867780765353 1104 54-55 -0.11442742915981796 1104 56-57 -0.1732518817814963 1104 58-59 -0.0789649847582794 1104 60-61 -0.16538643631249528 1104 62-63 -0.1407047911455166 1104 64-65 -0.17517890155602345 1104 66-67 -0.11033340622797994 1104 68-69 -0.1823870059636974 1104 70-71 -0.14135692394622623 1104 72-73 -0.16293102279117733 1104 74-75 -0.09431151043867914 1104 76-77 -0.11011776279960017 1104 78-79 -0.10797408415901089 1104 80-81 -0.1314757588567339 1104 82-83 -0.1537714121568854 1104 84-85 -0.08407296332959646 1104 86-87 -0.11772785180977863 1104 88-89 -0.17960604936351388 1104 90-91 -0.24143371702902172 1104 92-93 -0.25913966626570684 1104 94-95 -0.2862656481014092 1104 96-97 -0.2845603252515154 1104 98-99 -0.3915382269941823 1104 100-101 -0.4138742650875038 1105 1 0.23179716677809736 1105 2 0.2476636220624755 1105 3 0.2337432037657905 1105 4 0.2281286155675275 1105 5 0.2522677414722949 1105 6 0.2748429547813842 1105 7 0.25295519103951136 1105 8 0.25571107279348837 1105 9 0.30991138654137274 1105 10-11 0.278692387891887 1105 12-13 0.24920727065031656 1105 14-15 0.35976089836015746 1105 16-17 0.33441598222854907 1105 18-19 0.3096325823796917 1105 20-21 0.3377861151230874 1105 22-23 0.252650125785685 1105 24-25 0.3403350445387474 1105 26-27 0.3539302796567867 1105 28-29 0.3456253185239291 1105 30-31 0.3740249625574563 1105 32-33 0.35438013146792 1105 34-35 0.42005569626805794 1105 36-37 0.3691730228611405 1105 38-39 0.3385791406262726 1105 40-41 0.37153175828889573 1105 42-43 0.4402343841363887 1105 44-45 0.37790105301785815 1105 46-47 0.44404165661551787 1105 48-49 0.3981595538896272 1105 50-51 0.3392500836813781 1105 52-53 0.37422010827430796 1105 54-55 0.5151944329436731 1105 56-57 0.5155812813996903 1105 58-59 0.57192339289346 1105 60-61 0.5397964800184525 1105 62-63 0.5498825786484005 1105 64-65 0.5275414070370772 1105 66-67 0.5693426285150238 1105 68-69 0.5480723490557864 1105 70-71 0.5400531716239598 1105 72-73 0.6104447768854868 1105 74-75 0.5553370110398106 1105 76-77 0.565957636503704 1105 78-79 0.523180895824467 1105 80-81 0.5352049730295718 1105 82-83 0.521696599221297 1105 84-85 0.4696114251562413 1105 86-87 0.4625097601143793 1105 88-89 0.5153993137278015 1105 90-91 0.4598303505595531 1105 92-93 0.49789730060713566 1105 94-95 0.43954589290207835 1105 96-97 0.4176217003954221 1105 98-99 0.41232809037874674 1105 100-101 0.3816526678861365 1106 1 0.27825396610913344 1106 2 0.3184589141571301 1106 3 0.3352739282131161 1106 4 0.364643620399427 1106 5 0.3842133431046548 1106 6 0.3528786331943152 1106 7 0.4019225877851369 1106 8 0.4122891031307532 1106 9 0.4500035093911592 1106 10-11 0.4571749925193913 1106 12-13 0.5046743861749476 1106 14-15 0.4987086107176921 1106 16-17 0.5089932479388182 1106 18-19 0.5055172629608649 1106 20-21 0.5150591126966546 1106 22-23 0.5501560815943094 1106 24-25 0.5299823062723448 1106 26-27 0.5236074669365607 1106 28-29 0.5366284633119776 1106 30-31 0.5386288085707491 1106 32-33 0.543289288114174 1106 34-35 0.555943397731383 1106 36-37 0.5644375360158094 1106 38-39 0.5391534866550174 1106 40-41 0.6242324204394407 1106 42-43 0.6156614108034049 1106 44-45 0.540451304646318 1106 46-47 0.5292761598901663 1106 48-49 0.4745474027209724 1106 50-51 0.419617496039578 1106 52-53 0.5372477219914842 1106 54-55 0.5824497139192317 1106 56-57 0.679823410834004 1106 58-59 0.7060152156011981 1106 60-61 0.6621162255810304 1106 62-63 0.5814410337991163 1106 64-65 0.6401070874372579 1106 66-67 0.6416292892552562 1106 68-69 0.6527850765707015 1106 70-71 0.7042000686676673 1106 72-73 0.6646763797240283 1106 74-75 0.6737804696190182 1106 76-77 0.6721207009972758 1106 78-79 0.7052604153031012 1106 80-81 0.6347767191967577 1106 82-83 0.6801282770088619 1106 84-85 0.7223661474740304 1106 86-87 0.6532767052197421 1106 88-89 0.7158420989335639 1106 90-91 0.6681273969066304 1106 92-93 0.599741638896635 1106 94-95 0.6428528506402174 1106 96-97 0.6787546782435108 1106 98-99 0.6496009863191965 1106 100-101 0.617036777267586 1107 1 0.22493261380389384 1107 2 0.2126651342782253 1107 3 0.20230272437417796 1107 4 0.23240659406160802 1107 5 0.21449696435455934 1107 6 0.22912993585668318 1107 7 0.21108712429382592 1107 8 0.3058191685201024 1107 9 0.37236232021254523 1107 10-11 0.3194258611456817 1107 12-13 0.345755483260227 1107 14-15 0.44391393183946803 1107 16-17 0.39764399108308623 1107 18-19 0.3389448696604376 1107 20-21 0.4383050375127837 1107 22-23 0.44396243859721096 1107 24-25 0.4127005088816986 1107 26-27 0.43916676283969736 1107 28-29 0.4597989437504282 1107 30-31 0.47983278578512056 1107 32-33 0.4621497650450834 1107 34-35 0.422510022532002 1107 36-37 0.45464849738987567 1107 38-39 0.4749949228922006 1107 40-41 0.41262010838144647 1107 42-43 0.39538404148841977 1107 44-45 0.4693292749619218 1107 46-47 0.4440676858847752 1107 48-49 0.3312107008230072 1107 50-51 0.4003389902298622 1107 52-53 0.47111694350368083 1107 54-55 0.545176312255478 1107 56-57 0.44562469873470434 1107 58-59 0.5039036807865287 1107 60-61 0.5489751660749178 1107 62-63 0.5749284502891285 1107 64-65 0.5851546429919239 1107 66-67 0.5875274108987227 1107 68-69 0.550022329320818 1107 70-71 0.6125709007682261 1107 72-73 0.5497423934225552 1107 74-75 0.6050519029207493 1107 76-77 0.6970374905194419 1107 78-79 0.6142572061594649 1107 80-81 0.5750558048119601 1107 82-83 0.5855367918212373 1107 84-85 0.6457647026785729 1107 86-87 0.5920330124383248 1107 88-89 0.6549031180506759 1107 90-91 0.6927661320714797 1107 92-93 0.6492932844918258 1107 94-95 0.6746180633036438 1107 96-97 0.7211323894780044 1107 98-99 0.7930718045183518 1107 100-101 0.8260528896955925 1108 1 0.10916178499912021 1108 2 0.1488293262916045 1108 3 0.1376959687475896 1108 4 0.17031468632007574 1108 5 0.15631802851493148 1108 6 0.16321874728512853 1108 7 0.230373922334131 1108 8 0.19703896970659684 1108 9 0.1808154067092218 1108 10-11 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-0.4771672591344256 2316 92-93 -0.6473441744002422 2316 94-95 -0.9182966690930705 2316 96-97 -0.6343595022486284 2316 98-99 -0.42551349153459483 2316 100-101 -0.3860140936957528 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 96964.0 3 21374.0 4 16396.0 5 14534.0 6 14679.0 7 16025.0 8 18580.0 9 21561.0 10 25641.0 11 30329.0 12 36167.0 13 41700.0 14 46936.0 15 55694.0 16 67346.0 17 84022.0 18 69215.0 19 44071.0 20 41070.0 21 41721.0 22 44052.0 23 48102.0 24 55682.0 25 65657.0 26 79118.0 27 96237.0 28 116351.0 29 143078.0 30 177295.0 31 224331.0 32 290003.0 33 386769.0 34 541919.0 35 809933.0 36 1248895.0 37 1856174.0 38 1926635.0 39 486539.0 40 245.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.34963783033102 20.5012948233238 15.361470713332647 21.787596633012537 2 15.401056162021293 20.480635309868763 40.806366289277506 23.311942238832437 3 17.847340764065272 26.385938307287905 31.031672252959332 24.735048675687494 4 12.43427102819148 17.71583213083085 39.42375620110983 30.42614063986784 5 12.822406277597404 37.73732303888374 35.38819167827739 14.052079005241472 6 29.267200359473527 38.582949644558205 18.16435956969555 13.985490426272715 7 26.059054051452236 33.568962929451644 22.801636394252345 17.570346624843776 8 25.19365183241896 35.46930983591107 20.487618175540074 18.849420156129895 9 25.098752018736896 17.21021810390379 22.01768690649986 35.673342970859444 10-11 23.59354753126233 27.342107029605533 28.72121217343443 20.343133265697706 12-13 24.776568454213002 24.900948118350676 28.934734239263094 21.38774918817323 14-15 21.999847246035365 26.56973314222777 27.068300382374233 24.36211922936263 16-17 20.719828825598206 30.018777782517493 27.5564383954804 21.704954996403902 18-19 20.65901521894705 28.224741619367027 30.727428277982803 20.38881488370312 20-21 21.68537036449035 27.342475070467625 30.64738627171503 20.324768293326997 22-23 21.39594021751213 27.25683380621123 30.697652055691837 20.64957392058481 24-25 21.09474265153301 27.358688973406036 30.659951439992305 20.88661693506865 26-27 20.894133194963985 27.46527310181997 30.636939382354463 21.003654320861585 28-29 20.937775453901757 27.413581835600738 30.77253116849071 20.876111542006797 30-31 21.19798857022748 27.692320620590984 30.4094219168449 20.70026889233664 32-33 20.89090215055782 27.629883628528013 30.593577531225378 20.885636689688784 34-35 21.198285261439125 27.393256992718744 30.489907827483687 20.918549918358444 36-37 21.448723364863458 27.554938995497718 30.07913227923973 20.917205360399098 38-39 21.228219023272754 27.85958290642222 30.137692663142452 20.774505407162575 40-41 21.168175585616922 27.585798822862383 30.252920771383625 20.993104820137074 42-43 21.168931893008995 27.73158171478391 29.97413611441552 21.125350277791576 44-45 21.320519176024543 27.839021816371428 29.758757185956163 21.081701821647865 46-47 21.568263547078406 27.92575764635943 29.354521026304877 21.15145778025729 48-49 21.545956446209214 28.059223002857337 29.125805745235922 21.269014805697527 50-51 21.558784080526998 28.168849349040837 28.87405311295722 21.398313457474938 52-53 21.59836173921906 28.184996273029068 28.833222718389244 21.383419269362626 54-55 21.52038698113556 28.525428282424148 28.720598592495104 21.23358614394519 56-57 21.531572381370037 28.242596605872933 28.787604705174008 21.438226307583026 58-59 21.41923324397667 27.957403896995892 29.01563118411577 21.60773167491167 60-61 21.628727036773075 28.17986575753385 28.850700464849112 21.340706740843963 62-63 21.415295205664368 28.232974165395675 28.910469553555114 21.441261075384848 64-65 21.549819764898157 28.096007383219497 28.924667609922032 21.42950524196032 66-67 21.45379081765322 28.13047543831031 28.831363454246418 21.58437028979005 68-69 21.72193332387319 28.331029994534536 28.59883567925695 21.348201002335323 70-71 21.720720576972173 28.13337694664284 28.66450195728023 21.481400519104756 72-73 21.48688194886852 28.285177133553475 28.757413784061765 21.470527133516246 74-75 21.57917850576034 28.392629989006885 28.57094305464865 21.457248450584128 76-77 21.700381713089882 28.393472133838088 28.385977655497395 21.52016849757464 78-79 21.69716448503693 28.360479176130998 28.51806245174089 21.424293887091185 80-81 21.63991104948354 28.380935065548506 28.519868384895773 21.45928550007218 82-83 21.56868366958678 28.4039135146602 28.622508353164445 21.404894462588576 84-85 21.531724889525492 28.41080586671912 28.4805646831245 21.57690456063089 86-87 21.612595485825775 28.52039241830124 28.40564294649241 21.46136914938058 88-89 21.509475709245464 28.42038359183671 28.49602651278529 21.57411418613254 90-91 21.412765760994265 28.37421483967247 28.524271425360453 21.688747973972816 92-93 21.496460066147552 28.521673734656144 28.350792113884204 21.6310740853121 94-95 21.434899454292974 28.300012227522114 28.569145361091387 21.69594295709352 96-97 21.544308051046976 28.36757510913484 28.410959389345912 21.677157450472272 98-99 21.467556349419993 28.41770155289181 28.33893387530857 21.775808222379624 100-101 21.482222855558046 28.390404302655238 28.29656882691985 21.830804014866867 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3561.0 1 2342.0 2 1972.5 3 2820.5 4 2107.5 5 1672.0 6 2930.5 7 5979.0 8 10609.0 9 13424.5 10 11546.0 11 9083.0 12 9104.5 13 9884.5 14 10666.5 15 11414.0 16 12268.5 17 13173.5 18 14431.0 19 16088.5 20 18266.0 21 21797.0 22 27672.5 23 37045.5 24 48603.5 25 63008.0 26 82528.5 27 106264.5 28 133198.5 29 160563.0 30 184881.5 31 206036.0 32 225063.0 33 243887.5 34 261566.0 35 275556.0 36 285875.5 37 295687.5 38 307801.0 39 313742.5 40 317600.5 41 322634.5 42 322041.0 43 321644.5 44 317806.0 45 311012.5 46 306141.0 47 297741.5 48 290580.0 49 286211.0 50 277822.0 51 264277.0 52 246792.5 53 230781.5 54 218147.5 55 207583.0 56 193865.5 57 174929.5 58 157163.0 59 141786.0 60 126220.5 61 109093.0 62 93196.0 63 78690.5 64 64834.5 65 53442.5 66 43565.0 67 34881.0 68 27599.0 69 21543.5 70 16807.0 71 13117.5 72 10581.5 73 8436.0 74 6650.5 75 5155.5 76 3794.5 77 3029.0 78 2531.0 79 2050.5 80 1503.5 81 1091.0 82 851.5 83 637.5 84 464.0 85 373.5 86 318.5 87 265.0 88 236.0 89 222.0 90 203.5 91 151.0 92 124.0 93 112.0 94 93.5 95 80.5 96 63.0 97 54.0 98 41.0 99 18.5 100 10.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013849531541191187 2 0.004244211278752138 3 0.003733629470781956 4 0.00537174610468629 5 0.0026167317658471827 6 0.006658837745611124 7 0.0013509143669211065 8 0.00431867112574779 9 0.010413741458391837 10-11 0.007116233948584412 12-13 0.008398007029009558 14-15 0.003212410541812395 16-17 0.006052521848646533 18-19 0.00449418362223754 20-21 9.732965714431595E-4 22-23 9.892522529422276E-4 24-25 0.0075736301515577 26-27 0.0012126317939291822 28-29 8.45651119450614E-4 30-31 6.967314254593109E-4 32-33 0.0016327980734046445 34-35 0.002021052989881971 36-37 0.0014413298954158264 38-39 0.0018721332958906674 40-41 0.002872022669832274 42-43 0.0030528537268217134 44-45 0.008084211959527883 46-47 0.014232467897168824 48-49 0.005355790423187221 50-51 0.006015291925148707 52-53 0.013993132674682801 54-55 0.008387369908010178 56-57 0.005717452537166101 58-59 0.0074087547760673285 60-61 0.001489196939913031 62-63 0.0025316347978521525 64-65 0.002797562822836622 66-67 0.002675235931343766 68-69 0.002063601473879486 70-71 0.0025529090398509102 72-73 0.0014785598189136521 74-75 0.0016859836784015386 76-77 0.008360777105511731 78-79 0.006525873733118889 80-81 0.012839005046250202 82-83 0.015514240977593968 84-85 0.00845119263400645 86-87 0.005967424880651502 88-89 0.0031113578923182964 90-91 0.0026911916128428345 92-93 9.307480874456443E-4 94-95 0.0012392245964276292 96-97 0.0018029920093947053 98-99 0.0064939623701207524 100-101 0.01145617931633096 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9401040.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.78308781745453 #Duplication Level Percentage of deduplicated Percentage of total 1 69.37599719322303 33.84375363500478 2 16.483714499765078 16.08252983999777 3 6.029567395703013 8.824227472975219 4 2.7846469820302775 5.43374713059971 5 1.4374552133623664 3.5061751953557074 6 0.8789798656466145 2.5727611185367905 7 0.5740946811381789 1.9604277871848135 8 0.40819835449328273 1.5930540939348994 9 0.29518270936994045 1.2959931630049168 >10 1.5614923650580856 13.808286039512746 >50 0.10945195909625462 3.6876271252293273 >100 0.05806634181942828 5.055546674118613 >500 0.0023211101903964607 0.7580022397396992 >1k 7.438208854825162E-4 0.6124839905999097 >5k 2.1877054683389362E-5 0.0761682250698283 >10k+ 6.563116405016809E-5 0.8892162691352241 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36639 0.38973347629623956 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30164 0.32085811782526186 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16517 0.1756933275467395 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.0637120999378792E-5 0.0 0.0 0.0 6 0.0 1.0637120999378792E-5 0.0 0.0 0.0 7 0.0 1.0637120999378792E-5 0.0 0.0 0.0 8 0.0 1.0637120999378792E-5 0.0 0.0 0.0 9 0.0 1.0637120999378792E-5 0.0 0.0 0.0 10-11 1.0637120999378792E-5 1.0637120999378792E-5 0.0 0.0 0.0 12-13 1.0637120999378792E-5 1.0637120999378792E-5 0.0 0.0 0.0 14-15 1.0637120999378792E-5 1.595568149906819E-5 0.0 5.318560499689396E-6 0.0 16-17 1.0637120999378792E-5 2.1274241998757584E-5 0.0 1.0637120999378792E-5 0.0 18-19 1.0637120999378792E-5 3.191136299813638E-5 0.0 1.595568149906819E-5 0.0 20-21 1.0637120999378792E-5 3.191136299813638E-5 0.0 2.1274241998757584E-5 0.0 22-23 1.0637120999378792E-5 3.191136299813638E-5 0.0 4.254848399751517E-5 0.0 24-25 1.0637120999378792E-5 3.191136299813638E-5 0.0 1.0637120999378792E-4 0.0 26-27 1.0637120999378792E-5 3.191136299813638E-5 0.0 2.1274241998757584E-4 0.0 28-29 1.0637120999378792E-5 5.318560499689396E-5 0.0 6.914128649596215E-4 0.0 30-31 1.0637120999378792E-5 5.850416549658336E-5 0.0 0.0018029920093947053 0.0 32-33 2.1274241998757584E-5 6.382272599627276E-5 0.0 0.0044144052147421994 0.0 34-35 2.1274241998757584E-5 6.382272599627276E-5 0.0 0.008270361577017011 0.0 36-37 2.1274241998757584E-5 7.445984699565154E-5 0.0 0.014684545539642423 0.0 38-39 2.1274241998757584E-5 8.509696799503034E-5 0.0 0.024694076400057864 0.0 40-41 2.1274241998757584E-5 8.509696799503034E-5 0.0 0.03599601746189783 0.0 42-43 2.1274241998757584E-5 1.0637120999378792E-4 0.0 0.0477500361662114 0.0 44-45 2.1274241998757584E-5 1.0637120999378792E-4 5.318560499689396E-6 0.0617963544458911 0.0 46-47 2.1274241998757584E-5 1.0637120999378792E-4 1.0637120999378792E-5 0.07998583135482883 0.0 48-49 2.1274241998757584E-5 1.1700833099316671E-4 1.0637120999378792E-5 0.10040910367363612 0.0 50-51 2.1274241998757584E-5 1.382825729919243E-4 1.0637120999378792E-5 0.12305553428131355 0.0 52-53 2.1274241998757584E-5 1.4891969399130308E-4 1.0637120999378792E-5 0.1450052334635317 0.0 54-55 2.1274241998757584E-5 1.6487537549037125E-4 1.0637120999378792E-5 0.1696833541820905 0.0 56-57 2.1274241998757584E-5 1.8083105698943946E-4 1.0637120999378792E-5 0.2003820853862977 0.0 58-59 2.1274241998757584E-5 1.9146817798881827E-4 1.0637120999378792E-5 0.2355324517287449 0.0 60-61 2.1274241998757584E-5 1.9146817798881827E-4 1.0637120999378792E-5 0.2727517381055713 0.0 62-63 2.1274241998757584E-5 1.9146817798881827E-4 1.0637120999378792E-5 0.3117155123262958 0.0 64-65 2.1274241998757584E-5 1.9146817798881827E-4 1.0637120999378792E-5 0.3537693701973399 0.0 66-67 2.1274241998757584E-5 1.9146817798881827E-4 1.0637120999378792E-5 0.40462012713487017 0.0 68-69 2.1274241998757584E-5 2.0210529898819705E-4 1.0637120999378792E-5 0.46378911269391476 0.0 70-71 2.1274241998757584E-5 2.0210529898819705E-4 1.0637120999378792E-5 0.5303296231055288 0.0 72-73 2.1274241998757584E-5 2.1806098048726523E-4 1.0637120999378792E-5 0.6028216027162951 0.0 74-75 2.1274241998757584E-5 2.2337954098695465E-4 1.0637120999378792E-5 0.6779834996979057 0.0 76-77 2.1274241998757584E-5 2.2337954098695465E-4 1.0637120999378792E-5 0.762431603311974 0.0 78-79 2.1274241998757584E-5 2.2337954098695465E-4 1.0637120999378792E-5 0.86078774263273 0.0 80-81 2.1274241998757584E-5 2.2869810148664404E-4 1.0637120999378792E-5 0.9721264881332278 0.0 82-83 2.1274241998757584E-5 2.3401666198633343E-4 1.0637120999378792E-5 1.091251606205271 0.0 84-85 2.1274241998757584E-5 2.4997234348540163E-4 1.0637120999378792E-5 1.2145358385880711 0.0 86-87 2.1274241998757584E-5 2.765651459838486E-4 1.0637120999378792E-5 1.3506643945776213 0.0 88-89 2.659280249844698E-5 2.872022669832274E-4 1.0637120999378792E-5 1.5057004331435673 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17605 0.0 56.478798 1 GTATCAA 34355 0.0 43.7106 1 ATCAACG 41925 0.0 35.3049 3 TCAACGC 42095 0.0 35.096115 4 TATCAAC 43670 0.0 34.023968 2 CAACGCA 43690 0.0 33.802 5 AACGCAG 45585 0.0 32.542885 6 ACGCAGA 50390 0.0 29.186749 7 CGCAGAG 51070 0.0 28.742315 8 GCAGAGT 55380 0.0 25.656216 9 GTGGTAT 7580 0.0 25.508062 1 GTACATG 31000 0.0 23.40074 1 TGGTATC 8175 0.0 22.835274 2 GAGTACT 36790 0.0 22.6602 12-13 TACATGG 30655 0.0 22.530172 2 CAGAGTA 53660 0.0 22.003057 10-11 AGAGTAC 50855 0.0 22.002363 10-11 ACATGGG 31210 0.0 20.958048 3 GTACTTT 39775 0.0 20.885738 14-15 AGTACTT 38035 0.0 20.301119 12-13 >>END_MODULE