FastQCFastQC Report
Wed 25 May 2016
SRR2049399_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049399_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9401040
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT358980.3818513696356999No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298610.31763507016245013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199200.21189145030762555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA179300.056.2426831
GTATCAA336050.041.9438441
ATCAACG431250.032.2603763
TCAACGC435650.031.8800354
TATCAAC448950.031.0947972
CAACGCA448700.030.995185
AACGCAG468100.029.7902036
ACGCAGA533500.025.9868377
CGCAGAG537300.025.6969728
GTGGTAT76700.025.0934661
TGGTATC79800.023.0948372
GCAGAGT591600.022.945239
AGAGTAC538550.021.5144810-11
GTACATG299900.021.2972621
GAGTACT390050.021.12078712-13
AGTACTT404700.020.38556112-13
TACATGG301250.020.2295272
CAGAGTA572700.020.1030310-11
GTACTTT415050.019.63128314-15
ACATGGG299700.019.3512083
Produced by FastQC (version 0.11.5)