FastQCFastQC Report
Wed 25 May 2016
SRR2049398_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049398_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7526137
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307460.40852299127693265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245710.32647558767532403No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129610.17221318187537643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148500.055.597331
GTATCAA286500.043.0106471
ATAACGC11400.037.084773
TATAACG11700.036.537692
ATCAACG342100.035.546543
TCAACGC346350.035.1108174
TATCAAC353250.034.4493752
CAACGCA358100.033.9711235
AACGCAG376100.032.7117656
ACGCAGA419050.029.1221287
CGCAGAG418250.029.0642538
GTGGTAT65050.028.6265741
GTACCTG58250.027.8907871
TGGTATC63450.027.548672
GCAGAGT442950.026.8108239
TACCTGG58350.026.7003772
TCTGTCG22250.025.406328
TAACGCA17400.025.3892524
GTCTCGC22050.024.5599161
GTACATG203250.023.55921
Produced by FastQC (version 0.11.5)