##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049398_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7526137 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.380528284297775 31.0 31.0 34.0 30.0 34.0 2 31.405057867004015 31.0 31.0 34.0 28.0 34.0 3 31.426089639346188 33.0 31.0 34.0 28.0 34.0 4 35.053833460645215 37.0 35.0 37.0 32.0 37.0 5 35.079313464530344 37.0 35.0 37.0 32.0 37.0 6 35.17160902067023 37.0 35.0 37.0 32.0 37.0 7 35.11846422673411 37.0 35.0 37.0 32.0 37.0 8 35.088147079969445 37.0 35.0 37.0 32.0 37.0 9 36.65558134272602 39.0 37.0 39.0 32.0 39.0 10-11 36.594282830620806 39.0 37.0 39.0 32.0 39.0 12-13 36.55260952597594 39.0 37.0 39.0 32.0 39.0 14-15 37.700583114551335 40.0 38.0 41.0 32.0 41.0 16-17 37.68656204371513 40.0 38.0 41.0 32.0 41.0 18-19 37.726406455263835 40.0 38.0 41.0 32.0 41.0 20-21 37.68322002376518 40.0 38.0 41.0 32.0 41.0 22-23 37.56147808630111 40.0 37.5 41.0 32.0 41.0 24-25 37.444269218059674 40.0 37.0 41.0 32.0 41.0 26-27 37.291923798357644 40.0 37.0 41.0 32.0 41.0 28-29 37.12285452683096 40.0 37.0 41.0 31.0 41.0 30-31 36.91306715516872 40.0 36.5 41.0 31.0 41.0 32-33 36.617803994266914 39.5 36.0 41.0 30.0 41.0 34-35 36.3972512060304 39.0 36.0 41.0 30.0 41.0 36-37 36.17916495540807 39.0 35.0 41.0 30.0 41.0 38-39 35.94637687302264 39.0 35.0 41.0 29.0 41.0 40-41 35.71464936128588 39.0 35.0 40.0 28.5 41.0 42-43 35.47366836399603 39.0 35.0 40.0 27.0 41.0 44-45 35.17096632708121 38.5 35.0 40.0 26.0 41.0 46-47 34.7976573506435 38.0 34.5 40.0 24.5 41.0 48-49 34.48249573453154 38.0 34.0 40.0 23.0 41.0 50-51 33.13230917800194 36.5 32.5 39.0 21.5 40.0 52-53 33.41749153649475 37.5 33.5 39.0 22.0 40.0 54-55 34.44768245648464 38.5 34.0 40.0 23.0 41.0 56-57 34.57140409482315 39.0 34.5 40.0 23.0 41.0 58-59 34.43202747970174 38.5 34.0 40.0 22.5 41.0 60-61 34.179530016527735 38.0 34.0 40.0 22.0 41.0 62-63 33.90635913218162 38.0 34.0 40.0 20.5 41.0 64-65 33.62433031713348 37.5 33.5 40.0 20.0 41.0 66-67 33.32209538572046 37.0 33.0 40.0 18.5 41.0 68-69 32.97883363005484 36.5 33.0 39.5 17.5 41.0 70-71 32.64047684755141 36.0 33.0 39.0 14.5 41.0 72-73 32.38465550122194 36.0 32.5 39.0 13.0 41.0 74-75 32.19782054193273 35.0 32.5 39.0 11.0 40.0 76-77 31.864187896127852 35.0 32.0 37.5 7.5 40.0 78-79 31.489050026593986 35.0 32.0 37.0 7.0 39.0 80-81 31.145327476765303 35.0 32.0 37.0 2.0 39.0 82-83 30.80609149155802 35.0 31.5 36.0 2.0 39.0 84-85 30.47793389889129 35.0 31.0 36.0 2.0 37.0 86-87 30.16845534435528 35.0 31.0 35.5 2.0 37.0 88-89 29.87327150701615 35.0 31.0 35.0 2.0 37.0 90-91 29.601114555847175 34.0 31.0 35.0 2.0 36.0 92-93 29.337059636304787 34.0 30.5 35.0 2.0 36.0 94-95 29.07019490875598 34.0 30.0 35.0 2.0 36.0 96-97 28.80834816055036 34.0 30.0 35.0 2.0 35.5 98-99 28.492093885614892 34.0 30.0 35.0 2.0 35.0 100-101 27.955835908913166 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14489093998614422 1101 2 0.18018994933016685 1101 3 0.1638499601595207 1101 4 0.16393727515713152 1101 5 0.10246085736757493 1101 6 0.16797341136192045 1101 7 0.1862424796235871 1101 8 0.18968295860116058 1101 9 0.2269824040156152 1101 10-11 0.2118519292045491 1101 12-13 0.20216648221902034 1101 14-15 0.334228754771587 1101 16-17 0.25733636369290736 1101 18-19 0.2446525421918082 1101 20-21 0.15237724851020573 1101 22-23 0.21934742398051554 1101 24-25 0.15713363406386804 1101 26-27 0.14015203604923698 1101 28-29 0.16878537352845058 1101 30-31 0.16549095355924948 1101 32-33 0.1396230992399481 1101 34-35 0.12050529876524507 1101 36-37 0.13474564336444672 1101 38-39 0.10198893441340573 1101 40-41 0.06731355108575343 1101 42-43 0.023337057682624618 1101 44-45 -0.11204601703136774 1101 46-47 -0.1751264563405357 1101 48-49 -0.2452532110457426 1101 50-51 -0.203254729227055 1101 52-53 -0.0940641929858117 1101 54-55 0.12864635770752386 1101 56-57 0.09376370767746067 1101 58-59 0.07811827839588403 1101 60-61 0.1267197221468166 1101 62-63 0.05885608551356114 1101 64-65 0.06318245318702509 1101 66-67 0.07370795338614045 1101 68-69 0.0184833112992564 1101 70-71 -0.013032003996073627 1101 72-73 -0.041134208938515826 1101 74-75 2.785586550402286E-5 1101 76-77 -0.030100939444519526 1101 78-79 -0.08363294741845095 1101 80-81 -0.1735170795449541 1101 82-83 -0.19157679378838566 1101 84-85 -0.24532657523354473 1101 86-87 -0.3081146139228004 1101 88-89 -0.3179187292061414 1101 90-91 -0.4199567875074379 1101 92-93 -0.4081611694580296 1101 94-95 -0.48844840862128436 1101 96-97 -0.6060025275615608 1101 98-99 -0.7914461903944812 1101 100-101 -0.8769829790545991 1102 1 0.2799719991374303 1102 2 0.3299416681181597 1102 3 0.30520098504120696 1102 4 0.3623432462945928 1102 5 0.3504027855544791 1102 6 0.33888011729805356 1102 7 0.3007788042066437 1102 8 0.3811567232362236 1102 9 0.4980726297020155 1102 10-11 0.4889153815448921 1102 12-13 0.4024244610906891 1102 14-15 0.5198991584416319 1102 16-17 0.4320015169494056 1102 18-19 0.37857325676162645 1102 20-21 0.4300221307757539 1102 22-23 0.3918275514761902 1102 24-25 0.3688601382922556 1102 26-27 0.39042594973143707 1102 28-29 0.3594353920626716 1102 30-31 0.2995038290150518 1102 32-33 0.35764796219896766 1102 34-35 0.3746120974064908 1102 36-37 0.3682375126367745 1102 38-39 0.2916163394804414 1102 40-41 0.201063853947403 1102 42-43 0.24853739213999404 1102 44-45 0.25779593170377524 1102 46-47 0.2432485463761367 1102 48-49 0.12711033507743963 1102 50-51 0.0691943593128812 1102 52-53 0.21187423344547085 1102 54-55 0.34664192360168045 1102 56-57 0.4336138492074397 1102 58-59 0.33913751943344295 1102 60-61 0.3627213196021728 1102 62-63 0.37586410311679685 1102 64-65 0.3398236894404505 1102 66-67 0.344632028104634 1102 68-69 0.30984683733608165 1102 70-71 0.2695690322087998 1102 72-73 0.3191633504199558 1102 74-75 0.27031301646980666 1102 76-77 0.29149474072340453 1102 78-79 0.22562533862036815 1102 80-81 0.15483970617980702 1102 82-83 0.0830784451909885 1102 84-85 0.05036139629210368 1102 86-87 0.022166504706984114 1102 88-89 0.020865027806490133 1102 90-91 -0.010421191725800583 1102 92-93 -0.13576760480851036 1102 94-95 -0.27540077057338763 1102 96-97 -0.27885299036567446 1102 98-99 -0.35582663812237314 1102 100-101 -0.3702168698133015 1103 1 0.26774503235163394 1103 2 0.2835363245179927 1103 3 0.32700324134902914 1103 4 0.2931673197396094 1103 5 0.28734124766064184 1103 6 0.2692197601499018 1103 7 0.28429681801016926 1103 8 0.2753386762912484 1103 9 0.3993457989651006 1103 10-11 0.3697011061026316 1103 12-13 0.3480861311364478 1103 14-15 0.3762205948833568 1103 16-17 0.4020880140371972 1103 18-19 0.35431154181456037 1103 20-21 0.3697330243082746 1103 22-23 0.30618596803959264 1103 24-25 0.3178763013826327 1103 26-27 0.37798183064710855 1103 28-29 0.3158943603051725 1103 30-31 0.3737878308042042 1103 32-33 0.28698127438063636 1103 34-35 0.29730328402521167 1103 36-37 0.34142273431368864 1103 38-39 0.2836145527395644 1103 40-41 0.29961569202129823 1103 42-43 0.27100083051088575 1103 44-45 0.1868992453993954 1103 46-47 0.17605817484369624 1103 48-49 0.25734650145781046 1103 50-51 0.18325333900487095 1103 52-53 0.288922096788248 1103 54-55 0.40457056466816255 1103 56-57 0.44009619923552634 1103 58-59 0.44864332746208646 1103 60-61 0.46073675606388775 1103 62-63 0.4087181587012978 1103 64-65 0.32695301657956577 1103 66-67 0.40185691363561915 1103 68-69 0.40373002600467345 1103 70-71 0.3462270698010528 1103 72-73 0.3974659586615843 1103 74-75 0.2796770422477124 1103 76-77 0.21475312531791602 1103 78-79 0.24841581161181736 1103 80-81 0.16601670836589832 1103 82-83 0.1210562759475522 1103 84-85 0.14439178157772048 1103 86-87 0.10974003404267663 1103 88-89 0.057219445325863205 1103 90-91 0.04306760864030679 1103 92-93 -0.029937875592349172 1103 94-95 0.0013214031187906983 1103 96-97 -0.004683543807022517 1103 98-99 -0.06936707154067179 1103 100-101 -0.07157145286743472 1104 1 -0.0557017412991172 1104 2 -0.07498171639640461 1104 3 -0.03071552354155571 1104 4 -0.06099509737482833 1104 5 -0.0736612247125521 1104 6 -0.06053429240492392 1104 7 -0.1277052210887959 1104 8 -0.15610919375255605 1104 9 -0.0938868212667785 1104 10-11 -0.055086948015819814 1104 12-13 -0.10752899819573258 1104 14-15 -0.11033976522481481 1104 16-17 -0.10964281700884015 1104 18-19 -0.1286070506582675 1104 20-21 -0.09804056709594988 1104 22-23 -0.0971927475956349 1104 24-25 -0.04341489182989022 1104 26-27 -0.09407811385400322 1104 28-29 -0.12099575819515707 1104 30-31 -0.11302502388974744 1104 32-33 -0.1746689138003461 1104 34-35 -0.17519003761692176 1104 36-37 -0.14919150136896775 1104 38-39 -0.19665647006547715 1104 40-41 -0.2545063907961733 1104 42-43 -0.22191740861875076 1104 44-45 -0.25222688371221125 1104 46-47 -0.15976384378880226 1104 48-49 -0.2854661377997232 1104 50-51 -0.38871890434361944 1104 52-53 -0.29610879329697326 1104 54-55 -0.14051543494829843 1104 56-57 -0.22300867371014732 1104 58-59 -0.15738646554398628 1104 60-61 -0.22213452958732205 1104 62-63 -0.28145257981685035 1104 64-65 -0.2828101302743278 1104 66-67 -0.2399148726308553 1104 68-69 -0.19485612952876608 1104 70-71 -0.2076991991383963 1104 72-73 -0.27594305357952464 1104 74-75 -0.3107470141801656 1104 76-77 -0.3268727182031981 1104 78-79 -0.34626794280025663 1104 80-81 -0.4124717494900345 1104 82-83 -0.3820734013471956 1104 84-85 -0.3393016873707886 1104 86-87 -0.32542246780551665 1104 88-89 -0.39468373658104383 1104 90-91 -0.38280582657250406 1104 92-93 -0.3864238415211467 1104 94-95 -0.4581689490683196 1104 96-97 -0.4374406961764983 1104 98-99 -0.4487831496164816 1104 100-101 -0.48446464579659576 1105 1 0.22410421289238514 1105 2 0.22323227461130912 1105 3 0.24898938839656637 1105 4 0.2563616616486897 1105 5 0.2641931147721195 1105 6 0.28110261545636916 1105 7 0.3439939015513289 1105 8 0.3198623406305927 1105 9 0.4197863704784339 1105 10-11 0.3834499293517055 1105 12-13 0.34484005590981326 1105 14-15 0.45821004978239444 1105 16-17 0.39127188618428477 1105 18-19 0.42303844613306296 1105 20-21 0.4347356527637345 1105 22-23 0.3513173386807438 1105 24-25 0.4197346429365396 1105 26-27 0.3644965340217894 1105 28-29 0.46836298112113184 1105 30-31 0.4157560411237 1105 32-33 0.38767166856150936 1105 34-35 0.4242821427004131 1105 36-37 0.4243716511310609 1105 38-39 0.3999284744052005 1105 40-41 0.47611774584896693 1105 42-43 0.4137787749611732 1105 44-45 0.44453282323681975 1105 46-47 0.4228606747520516 1105 48-49 0.3907875515753787 1105 50-51 0.3550176649279635 1105 52-53 0.46291863388646703 1105 54-55 0.5845882911870461 1105 56-57 0.6066063385487084 1105 58-59 0.6231241948961141 1105 60-61 0.5503135175578748 1105 62-63 0.49439831485475594 1105 64-65 0.5276432425931006 1105 66-67 0.49921981437417173 1105 68-69 0.5141476813551407 1105 70-71 0.4496482744913095 1105 72-73 0.5267502065281349 1105 74-75 0.5413615946316739 1105 76-77 0.5231584398765996 1105 78-79 0.5411524709811495 1105 80-81 0.5735469137419393 1105 82-83 0.532041554915299 1105 84-85 0.5024567843070749 1105 86-87 0.4003066032507654 1105 88-89 0.4724868364349888 1105 90-91 0.5032522092882914 1105 92-93 0.4704961895267523 1105 94-95 0.5065993181398021 1105 96-97 0.46399733311164226 1105 98-99 0.49444237603299257 1105 100-101 0.45536716336427574 1106 1 0.16907780591435184 1106 2 0.1839861205770319 1106 3 0.20213064866591424 1106 4 0.178028282192642 1106 5 0.211649527025088 1106 6 0.27756994267036106 1106 7 0.23516705993131382 1106 8 0.2331499480026693 1106 9 0.2755373997969315 1106 10-11 0.26186910045091594 1106 12-13 0.2840427484558816 1106 14-15 0.31550888865170634 1106 16-17 0.3029884815120383 1106 18-19 0.2926452847919663 1106 20-21 0.3174202974449827 1106 22-23 0.35052735305308147 1106 24-25 0.29533632847405755 1106 26-27 0.3722014724167977 1106 28-29 0.3634958313164418 1106 30-31 0.2866636420539521 1106 32-33 0.2404338187165962 1106 34-35 0.3297000485693431 1106 36-37 0.32145593264755945 1106 38-39 0.315483837603999 1106 40-41 0.342705059423281 1106 42-43 0.32138415094843253 1106 44-45 0.32543191748937517 1106 46-47 0.30339364381865863 1106 48-49 0.23820952082756008 1106 50-51 0.22629492222657888 1106 52-53 0.34176655996800065 1106 54-55 0.42214510215369216 1106 56-57 0.417781313158919 1106 58-59 0.3838257861366614 1106 60-61 0.35975712887661615 1106 62-63 0.32500193974814096 1106 64-65 0.3652055916731598 1106 66-67 0.34094406415372447 1106 68-69 0.3775884234907849 1106 70-71 0.44405431419860264 1106 72-73 0.4024859923164783 1106 74-75 0.3714151849397851 1106 76-77 0.4017323309608436 1106 78-79 0.3412534882021703 1106 80-81 0.32421006471711067 1106 82-83 0.3233099248120972 1106 84-85 0.3495235691111276 1106 86-87 0.3289555908141928 1106 88-89 0.40828628207491846 1106 90-91 0.29732867366455196 1106 92-93 0.31792500097411036 1106 94-95 0.313602315055153 1106 96-97 0.32188035826561645 1106 98-99 0.29092520149648493 1106 100-101 0.3389472972741636 1107 1 0.20597097749095283 1107 2 0.1898539047940062 1107 3 0.15725346791708716 1107 4 0.21339544346194828 1107 5 0.2340665916143223 1107 6 0.2161044125783178 1107 7 0.23473104758235763 1107 8 0.2564955836184595 1107 9 0.3527584057903468 1107 10-11 0.3185219553970384 1107 12-13 0.3472864104369364 1107 14-15 0.40027889534675865 1107 16-17 0.3406878332403025 1107 18-19 0.28662365210197294 1107 20-21 0.35158424467100247 1107 22-23 0.41465518501450305 1107 24-25 0.36647887567946924 1107 26-27 0.37797051449993546 1107 28-29 0.387484269994971 1107 30-31 0.3721647766504361 1107 32-33 0.3552201093060745 1107 34-35 0.4133359278007376 1107 36-37 0.4364918927224224 1107 38-39 0.531564581614127 1107 40-41 0.4899915498832996 1107 42-43 0.43033122671165813 1107 44-45 0.44156872892880017 1107 46-47 0.47039583625608117 1107 48-49 0.42214110970994767 1107 50-51 0.45376269962375915 1107 52-53 0.4928631390895646 1107 54-55 0.519371782857533 1107 56-57 0.4724779712346887 1107 58-59 0.5362666571252319 1107 60-61 0.5512391913641252 1107 62-63 0.610662011328948 1107 64-65 0.5657750326218576 1107 66-67 0.5777358555358205 1107 68-69 0.5906735697872989 1107 70-71 0.5878153813403841 1107 72-73 0.6036128326106507 1107 74-75 0.6155714362038935 1107 76-77 0.555914577079097 1107 78-79 0.5430707581816314 1107 80-81 0.5542497373316415 1107 82-83 0.5718375067979373 1107 84-85 0.6259892751287417 1107 86-87 0.5799495126763183 1107 88-89 0.5556002639906907 1107 90-91 0.5586277113533384 1107 92-93 0.5654895920712484 1107 94-95 0.5729021536743026 1107 96-97 0.5561338349843759 1107 98-99 0.5976855043412428 1107 100-101 0.6480593662059171 1108 1 0.23096169290123214 1108 2 0.21558590021967916 1108 3 0.232771856727215 1108 4 0.264678924105759 1108 5 0.2562955681629262 1108 6 0.2656534861512796 1108 7 0.2648028020727011 1108 8 0.25556070636633876 1108 9 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-0.7616682442517728 2316 98-99 -0.5528692753132205 2316 100-101 -0.4628567959654255 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 74312.0 3 16345.0 4 12829.0 5 11678.0 6 11987.0 7 13509.0 8 15375.0 9 18285.0 10 23018.0 11 27872.0 12 35258.0 13 41757.0 14 44142.0 15 50164.0 16 59862.0 17 73797.0 18 60015.0 19 37259.0 20 33915.0 21 33366.0 22 35269.0 23 38688.0 24 44132.0 25 52265.0 26 62325.0 27 75226.0 28 91092.0 29 112332.0 30 137896.0 31 173567.0 32 224447.0 33 298515.0 34 420104.0 35 623196.0 36 972219.0 37 1484189.0 38 1571477.0 39 414258.0 40 195.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.09917104528241 20.163151747523827 14.25347896131558 21.484198245878183 2 16.134767793580593 20.779347960696292 40.15916438961206 22.926719856111056 3 17.582500958031062 26.919835787363343 31.238416593895284 24.25924666071031 4 12.424088325756838 18.335468908021287 39.69972442383001 29.540718342391862 5 12.139448917140918 36.87155440338776 35.07757865826193 15.911418021209395 6 27.774171571808093 38.9168076408986 19.14750531086728 14.16151547642602 7 25.038785440378845 33.368659663407755 23.659743131163616 17.932811765049784 8 24.590935050018455 34.410526850090264 22.005091271093043 18.993446828798234 9 24.491441255521718 17.5092614420236 23.21789497435712 34.78140232809756 10-11 22.836640229744233 27.118147344273257 29.433109926589108 20.6121024993934 12-13 24.445422160311043 24.84863250851956 29.56369606628896 21.142249264880437 14-15 21.824862637453897 26.761715927969686 27.9102915045097 23.503129930066716 16-17 20.124560338149294 30.06019397608452 28.034254757383724 21.780990928382458 18-19 19.91800882562807 27.86623632539222 31.636906857866464 20.57884799111325 20-21 21.374538744774576 27.386211641898196 31.230384424586205 20.008865188741023 22-23 20.870880357424614 27.602820199805677 31.29531544549358 20.23098399727613 24-25 20.576499431837473 27.43729034410724 31.42606170119481 20.560148522860477 26-27 20.372313745134395 27.300959534523113 31.488730136479386 20.837996583863106 28-29 20.55332468029959 27.384838792679233 31.421580680327228 20.640255846693947 30-31 20.983727404764945 28.05704928549211 30.81102283005157 20.14820047969138 32-33 20.24885495747061 27.660384846965474 31.545487358840756 20.545272836723157 34-35 20.46447376236065 27.128995863806022 31.4487667487687 20.957763625064626 36-37 21.302776642090198 27.79502827053775 30.4875955234318 20.414599563940257 38-39 20.98470389012169 28.00485543546149 30.654663298165687 20.35577737625114 40-41 20.5921206923012 27.72637612835117 30.84664123897227 20.834861940375358 42-43 20.571296418897024 27.82123232387522 30.52449758571542 21.08297367151233 44-45 21.092317119687102 28.066723685804224 30.22385027739192 20.61710891711675 46-47 21.09578360868708 28.642884593815797 29.49908493007675 20.762246867420377 48-49 21.061828042716126 28.20693839412706 29.722429952738427 21.00880361041838 50-51 21.118309540248394 28.067867562368814 29.499718033473492 21.314104863909293 52-53 21.043561286858925 28.32478311049156 29.31000665103463 21.321648951614886 54-55 21.243948018820998 29.043231032917742 28.99323456422978 20.71958638403148 56-57 20.80069840284862 28.619056524987403 29.096577513780204 21.48366755838377 58-59 20.659421248415168 28.106432823991668 29.591949749851494 21.642196177741678 60-61 21.264052893756734 28.615093216476684 29.002814232736252 21.118039657030334 62-63 21.060193830576164 28.541438068848144 29.10678167844236 21.291586422133328 64-65 20.992258871099597 28.216882887978752 29.44275152855722 21.348106712364434 66-67 20.90359071261563 28.388779087021742 29.045754414786906 21.66187578557572 68-69 21.563807158544222 28.70550973025393 28.724696586247738 21.005986524954107 70-71 21.655233361405035 28.27381229200345 28.879734508500306 21.191219838091214 72-73 21.137588611584043 28.518867209400543 29.30384245565742 21.039701723357997 74-75 21.138489550014878 28.634348414509642 29.01687486559123 21.21028716988425 76-77 21.349433048843427 28.836275147112833 28.594072486192307 21.220219317851427 78-79 21.483540766852023 28.90192892974722 28.77960684721958 20.834923456181176 80-81 21.244770318591723 28.628166537334476 28.935274837710196 21.191788306363602 82-83 21.03421720754045 28.70411553996902 29.076759315429936 21.18490793706059 84-85 21.154442713814337 28.736172796598662 28.802586825080557 21.306797664506437 86-87 21.36405495907794 28.836002616896465 28.739852201291082 21.06009022273451 88-89 20.95623310172476 28.76222641868445 28.888118070943126 21.393422408647663 90-91 20.7509269441944 28.6398101711833 28.908720154968048 21.70054272965425 92-93 21.076215934384827 28.952637128783387 28.584443342496158 21.386703594335632 94-95 20.96215059295491 28.609190814394193 28.90578785432331 21.522870738327587 96-97 20.90451688417831 28.694928132763298 28.893473295326842 21.507081687731542 98-99 20.852624247550533 28.699392472817877 28.82379333193058 21.624189947701012 100-101 21.005738177694877 28.857818729715508 28.547020427163122 21.589422665426493 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2238.0 1 1543.5 2 2439.0 3 3463.0 4 2157.0 5 1600.5 6 2497.0 7 4517.5 8 7809.0 9 10018.0 10 9082.5 11 7860.5 12 8133.5 13 9048.0 14 10059.0 15 10741.0 16 11422.5 17 12268.5 18 13329.0 19 14997.0 20 17316.0 21 20550.0 22 26036.5 23 35240.0 24 45953.5 25 57889.0 26 75057.5 27 95750.0 28 120806.0 29 145970.0 30 163961.0 31 181057.5 32 199072.0 33 216294.0 34 230963.0 35 241005.5 36 246712.5 37 250848.0 38 255925.5 39 256355.5 40 254192.5 41 249961.5 42 241935.5 43 234622.0 44 228630.5 45 223722.0 46 219452.0 47 215785.5 48 214338.5 49 214972.5 50 212532.5 51 203689.5 52 190432.0 53 177447.0 54 168832.5 55 163408.5 56 152877.0 57 139576.0 58 128278.5 59 116475.5 60 102986.5 61 88010.0 62 73320.5 63 59456.5 64 47768.5 65 38332.5 66 30417.0 67 23665.5 68 18162.0 69 13910.5 70 10682.0 71 8281.5 72 6587.0 73 5309.0 74 4281.5 75 3427.0 76 2668.5 77 2154.0 78 1788.5 79 1471.5 80 1166.0 81 805.5 82 600.5 83 511.5 84 414.5 85 341.0 86 283.0 87 237.0 88 194.0 89 175.0 90 155.0 91 112.0 92 90.0 93 76.5 94 73.5 95 63.0 96 42.0 97 31.0 98 27.5 99 17.5 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014854898336291248 2 0.004424580631471365 3 0.0037868032431511676 4 0.005979163015501844 5 0.003016155565597597 6 0.007042125329368838 7 0.0015545823840304794 8 0.00418541411085129 9 0.011307261613760153 10-11 0.007321152936758925 12-13 0.008776082603864373 14-15 0.0033217572308343575 16-17 0.006205042507198581 18-19 0.00434485845793134 20-21 9.367355390952888E-4 22-23 9.898836547886387E-4 24-25 0.007460666740453967 26-27 0.0012224066609470437 28-29 8.968744523252765E-4 30-31 6.377773883201967E-4 32-33 0.0014150685803354364 34-35 0.0018070359335738907 36-37 0.0013818510080270928 38-39 0.001840253505882234 40-41 0.0029497204209809097 42-43 0.0026773363280524925 44-45 0.007885851666000764 46-47 0.013672352762114218 48-49 0.004796617441324813 50-51 0.005593839176725058 52-53 0.013180732691950732 54-55 0.008045296013080815 56-57 0.005241732910256616 58-59 0.006743167178593746 60-61 0.0014217120947971052 62-63 0.0024315262929707495 64-65 0.00222557734465902 66-67 0.002757058501592517 68-69 0.0020129848818856207 70-71 0.002272081945890701 72-73 0.001375207493565424 74-75 0.0016143740141854978 76-77 0.008251244961392545 78-79 0.006816245837672102 80-81 0.01250973773132219 82-83 0.014908046451984595 84-85 0.0082180273890842 86-87 0.005693491893650089 88-89 0.002936433392057572 90-91 0.00263747524128248 92-93 9.699531114036324E-4 94-95 0.0013419899212570804 96-97 0.0018801145926522465 98-99 0.006185111963813574 100-101 0.011141173752218436 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7526137.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.556096934688284 #Duplication Level Percentage of deduplicated Percentage of total 1 74.0455852061912 42.61774879713261 2 15.544337714827503 17.893428166002852 3 4.941406510633567 8.53224216359176 4 1.9754169097301981 4.547891485714146 5 0.9912128582582682 2.8525171676411163 6 0.5851009546387663 2.020567635706049 7 0.38495711840189284 1.5509640455706333 8 0.25797839740019796 1.1878583718257063 9 0.19542625264604185 1.012317510679062 >10 0.9869898889195385 10.141107820273586 >50 0.060896611772473706 2.4030450150569482 >100 0.028146191447419158 2.84763903518398 >500 0.0017355941572481986 0.6707584886264106 >1k 6.941065544888199E-4 0.6642763402118568 >5k 4.6273768502011826E-5 0.1484011658028448 >10k+ 6.941065275301774E-5 0.9092367909804949 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30746 0.40852299127693265 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24571 0.32647558767532403 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12961 0.17221318187537643 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.328702892333743E-5 0.0 5 0.0 0.0 0.0 2.657405784667486E-5 0.0 6 0.0 0.0 0.0 2.657405784667486E-5 0.0 7 0.0 0.0 0.0 2.657405784667486E-5 0.0 8 0.0 0.0 0.0 5.314811569334972E-5 0.0 9 0.0 0.0 0.0 5.314811569334972E-5 0.0 10-11 1.328702892333743E-5 0.0 0.0 5.314811569334972E-5 0.0 12-13 1.328702892333743E-5 0.0 0.0 5.314811569334972E-5 0.0 14-15 1.328702892333743E-5 3.3217572308343576E-5 0.0 5.314811569334972E-5 0.0 16-17 1.328702892333743E-5 7.972217354002459E-5 0.0 5.314811569334972E-5 0.0 18-19 1.328702892333743E-5 8.63656880016933E-5 0.0 5.314811569334972E-5 0.0 20-21 1.328702892333743E-5 9.300920246336201E-5 0.0 6.643514461668715E-5 0.0 22-23 1.328702892333743E-5 9.300920246336201E-5 0.0 9.965271692503072E-5 0.0 24-25 1.328702892333743E-5 1.262267747717056E-4 0.0 2.3252300615840505E-4 0.0 26-27 1.328702892333743E-5 1.4615731815671173E-4 0.0 4.318284400084665E-4 0.0 28-29 1.328702892333743E-5 1.4615731815671173E-4 0.0 8.038652498619145E-4 0.0 30-31 1.328702892333743E-5 1.4615731815671173E-4 0.0 0.0019000451360372526 0.0 32-33 1.328702892333743E-5 1.4615731815671173E-4 0.0 0.004205344654236297 0.0 34-35 1.328702892333743E-5 1.4615731815671173E-4 0.0 0.008463837424165944 0.0 36-37 1.328702892333743E-5 1.5944434708004918E-4 0.0 0.014715384532596205 0.0 38-39 3.9861086770012294E-5 1.793748904650553E-4 0.0 0.024521211878019228 0.0 40-41 3.9861086770012294E-5 1.8601840492672402E-4 0.0 0.03839287007398351 0.0 42-43 3.9861086770012294E-5 1.9930543385006147E-4 0.0 0.05322783786688975 0.0 44-45 3.9861086770012294E-5 1.9930543385006147E-4 0.0 0.06981004996321485 0.0 46-47 3.9861086770012294E-5 2.3916652062007376E-4 0.0 0.08943499168298424 0.0 48-49 3.9861086770012294E-5 2.524535495434112E-4 0.0 0.11280023204467313 0.0 50-51 3.9861086770012294E-5 2.524535495434112E-4 0.0 0.13935435934796297 0.0 52-53 3.9861086770012294E-5 2.524535495434112E-4 0.0 0.16616758371525792 0.0 54-55 3.9861086770012294E-5 2.524535495434112E-4 0.0 0.19601689419153545 0.0 56-57 3.9861086770012294E-5 2.524535495434112E-4 0.0 0.23086212754298785 0.0 58-59 3.9861086770012294E-5 2.524535495434112E-4 0.0 0.2700522193523716 0.0 60-61 3.9861086770012294E-5 2.657405784667486E-4 0.0 0.31233154538643126 0.0 62-63 3.9861086770012294E-5 2.657405784667486E-4 0.0 0.3574144345233152 0.0 64-65 4.6504601231681005E-5 2.657405784667486E-4 0.0 0.4109611610843651 0.0 66-67 5.314811569334972E-5 2.7902760739008603E-4 0.0 0.4736958681459027 0.0 68-69 5.314811569334972E-5 2.9231463631342345E-4 0.0 0.5409415215269135 0.0 70-71 5.314811569334972E-5 2.9231463631342345E-4 0.0 0.6175146692121071 0.0 72-73 5.314811569334972E-5 3.0560166523676093E-4 0.0 0.7076405863991049 0.0 74-75 5.314811569334972E-5 3.0560166523676093E-4 0.0 0.8020781444717258 0.0 76-77 5.314811569334972E-5 3.0560166523676093E-4 0.0 0.8998308162607191 0.0 78-79 5.314811569334972E-5 3.0560166523676093E-4 0.0 1.011873687656762 0.0 80-81 5.314811569334972E-5 3.0560166523676093E-4 0.0 1.1372434490629124 0.0 82-83 5.314811569334972E-5 3.0560166523676093E-4 0.0 1.2766509565265687 0.0 84-85 5.314811569334972E-5 3.0560166523676093E-4 0.0 1.4205162621940048 0.0 86-87 5.314811569334972E-5 3.1224517969842964E-4 0.0 1.5780937817103249 1.328702892333743E-5 88-89 5.314811569334972E-5 3.1888869416009835E-4 0.0 1.758000153332314 1.328702892333743E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14850 0.0 55.59733 1 GTATCAA 28650 0.0 43.010647 1 ATAACGC 1140 0.0 37.08477 3 TATAACG 1170 0.0 36.53769 2 ATCAACG 34210 0.0 35.54654 3 TCAACGC 34635 0.0 35.110817 4 TATCAAC 35325 0.0 34.449375 2 CAACGCA 35810 0.0 33.971123 5 AACGCAG 37610 0.0 32.711765 6 ACGCAGA 41905 0.0 29.122128 7 CGCAGAG 41825 0.0 29.064253 8 GTGGTAT 6505 0.0 28.626574 1 GTACCTG 5825 0.0 27.890787 1 TGGTATC 6345 0.0 27.54867 2 GCAGAGT 44295 0.0 26.810823 9 TACCTGG 5835 0.0 26.700377 2 TCTGTCG 2225 0.0 25.40632 8 TAACGCA 1740 0.0 25.389252 4 GTCTCGC 2205 0.0 24.559916 1 GTACATG 20325 0.0 23.5592 1 >>END_MODULE