FastQCFastQC Report
Wed 25 May 2016
SRR2049398_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049398_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7526137
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302990.4025836893482009No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246180.32710007803472085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159940.21251274059985886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147600.055.187111
TATAACG11100.044.0754282
ATAACGC10650.043.7069133
GTATCAA280200.042.3170741
ATCAACG349250.033.3198743
TCAACGC352500.032.9722444
CAACGCA363600.032.2005925
TATCAAC362300.032.1858862
AACGCAG383950.030.7283366
TAACGCA17850.029.8025514
GTACCTG58800.028.3689331
TACCTGG57700.028.0711942
TCTGTCG20700.027.3042418
ACGCAGA433750.027.188847
CGCAGAG434250.027.05918
GCAGAGT466400.024.8173989
GTATAAC27750.023.6335681
GTGGTAT67900.023.377061
TGGTATC68250.022.618462
AGAGTAC432900.022.58512510-11
Produced by FastQC (version 0.11.5)