##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049398_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7526137 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07504567084017 33.0 31.0 34.0 30.0 34.0 2 32.26339727804583 34.0 31.0 34.0 30.0 34.0 3 32.333773488311465 34.0 31.0 34.0 30.0 34.0 4 35.97021034828359 37.0 35.0 37.0 35.0 37.0 5 35.862788041195635 37.0 35.0 37.0 35.0 37.0 6 35.889415114287715 37.0 35.0 37.0 35.0 37.0 7 35.82007834829475 37.0 35.0 37.0 35.0 37.0 8 35.807566218898224 37.0 35.0 37.0 35.0 37.0 9 37.41972754415711 39.0 37.0 39.0 34.0 39.0 10-11 37.41898772238666 39.0 37.0 39.0 34.0 39.0 12-13 37.400115889466264 39.0 37.0 39.0 34.0 39.0 14-15 38.628041583617204 40.0 38.0 41.0 34.0 41.0 16-17 38.62112249617566 40.0 38.0 41.0 34.0 41.0 18-19 38.66582511054476 40.0 38.0 41.0 34.0 41.0 20-21 38.65345548453344 40.0 38.0 41.0 34.0 41.0 22-23 38.557338446004906 40.0 38.0 41.0 34.0 41.0 24-25 38.45714235603205 40.0 38.0 41.0 34.0 41.0 26-27 38.27339078467479 40.0 38.0 41.0 34.0 41.0 28-29 38.08109983913394 40.0 38.0 41.0 33.0 41.0 30-31 37.87581643810098 40.0 37.0 41.0 33.0 41.0 32-33 37.589976438111606 40.0 37.0 41.0 33.0 41.0 34-35 37.39403966470448 40.0 36.0 41.0 32.5 41.0 36-37 37.1793928279541 39.5 36.0 41.0 31.5 41.0 38-39 37.29318301008871 40.0 37.0 41.0 32.0 41.0 40-41 37.18247010385275 40.0 37.0 41.0 32.0 41.0 42-43 36.982478992875095 40.0 36.0 41.0 31.0 41.0 44-45 36.730484576616135 40.0 36.0 41.0 31.0 41.0 46-47 36.382520607849685 40.0 36.0 41.0 30.0 41.0 48-49 36.08732022815955 39.0 35.5 41.0 29.5 41.0 50-51 35.8506344622746 39.0 35.0 41.0 28.5 41.0 52-53 35.66552568734797 39.0 35.0 40.5 28.0 41.0 54-55 35.5553799379416 39.0 35.0 40.0 28.0 41.0 56-57 35.47596416328855 39.0 35.0 40.0 28.0 41.0 58-59 35.25283415648692 39.0 35.0 40.0 27.0 41.0 60-61 35.00941366068675 38.0 34.5 40.0 26.5 41.0 62-63 34.7221492911968 38.0 34.0 40.0 26.0 41.0 64-65 34.468980500886445 37.5 34.0 40.0 26.0 41.0 66-67 34.15110480981146 37.0 34.0 40.0 25.5 41.0 68-69 33.8333114983158 36.5 34.0 39.5 25.0 41.0 70-71 33.49798003942793 36.0 33.0 39.0 24.5 41.0 72-73 33.13734263673383 36.0 33.0 39.0 23.5 40.5 74-75 32.77739077829702 35.0 33.0 38.5 22.5 40.0 76-77 30.78509267104758 33.5 30.0 36.0 20.5 38.5 78-79 32.088416540916015 35.0 32.0 37.0 22.5 39.0 80-81 32.101541069475616 35.0 33.0 37.0 22.5 39.0 82-83 31.834513907998222 35.0 33.0 36.0 22.0 39.0 84-85 31.559487955108978 35.0 32.0 36.0 21.0 37.0 86-87 31.28354260625338 35.0 32.0 35.5 20.0 37.0 88-89 31.01609716910548 35.0 32.0 35.0 19.5 37.0 90-91 30.75316659795058 35.0 32.0 35.0 18.0 36.0 92-93 30.499733262894363 35.0 32.0 35.0 15.5 36.0 94-95 30.239776528117943 34.0 31.0 35.0 8.5 36.0 96-97 30.00094052234234 34.0 31.0 35.0 2.0 35.5 98-99 29.725658727711178 34.0 31.0 35.0 2.0 35.0 100-101 29.12055135323739 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3766677611388829 1101 2 0.3830258477186561 1101 3 0.3740244907490151 1101 4 0.31388091618710945 1101 5 0.319886197716464 1101 6 0.32495993908001 1101 7 0.29304720366495474 1101 8 0.3055133715834941 1101 9 0.3999032881098543 1101 10-11 0.35219811626288333 1101 12-13 0.32403315491003326 1101 14-15 0.40460500071531413 1101 16-17 0.4124632486237729 1101 18-19 0.37406545891361276 1101 20-21 0.33514952444576096 1101 22-23 0.27367325984874213 1101 24-25 0.23873100143175208 1101 26-27 0.21095955692185697 1101 28-29 0.2513150233259367 1101 30-31 0.23099641464029474 1101 32-33 0.2774347900517853 1101 34-35 0.24836828920300036 1101 36-37 0.17821876631645495 1101 38-39 0.300636202051038 1101 40-41 0.3134391143817652 1101 42-43 0.3500629897420353 1101 44-45 0.30679545704918354 1101 46-47 0.4023766226558507 1101 48-49 0.28014660302521577 1101 50-51 0.2780613903803797 1101 52-53 0.22852489139204835 1101 54-55 0.29504790834621986 1101 56-57 0.21022357017893967 1101 58-59 0.23830518484317764 1101 60-61 0.17244524512193493 1101 62-63 0.1721881363385407 1101 64-65 0.08821470537605336 1101 66-67 0.023192788602919734 1101 68-69 0.052119989069623784 1101 70-71 0.0980950957430764 1101 72-73 0.057095648755442596 1101 74-75 0.006349006981942296 1101 76-77 0.0454854376528715 1101 78-79 0.1068052730368052 1101 80-81 0.07243597381519606 1101 82-83 0.03778346418585343 1101 84-85 0.0346378646207377 1101 86-87 0.03521639410925914 1101 88-89 -0.04399892258630089 1101 90-91 -0.058269123670143586 1101 92-93 -0.1343861112808753 1101 94-95 -0.13645416775688446 1101 96-97 -0.21323849439321307 1101 98-99 -0.17471838510754623 1101 100-101 -0.26262075182630795 1102 1 0.3362878126694895 1102 2 0.3907263838450916 1102 3 0.34837701845465574 1102 4 0.3055615522934261 1102 5 0.29472205090768 1102 6 0.2788373362431926 1102 7 0.30479572947567846 1102 8 0.29751243536638583 1102 9 0.40846535307797893 1102 10-11 0.29927154088370855 1102 12-13 0.30084406708833455 1102 14-15 0.37081879843162113 1102 16-17 0.4032876639114633 1102 18-19 0.33885516685563033 1102 20-21 0.31637089356912185 1102 22-23 0.34313271584387195 1102 24-25 0.32137960575825275 1102 26-27 0.28915878476831125 1102 28-29 0.3152696387083793 1102 30-31 0.31270186472502814 1102 32-33 0.36152602195317485 1102 34-35 0.34892392553757645 1102 36-37 0.40113836052641005 1102 38-39 0.4223600284578879 1102 40-41 0.44983415539569194 1102 42-43 0.5239380173607628 1102 44-45 0.5375028430495874 1102 46-47 0.5149248553992578 1102 48-49 0.32808565968800707 1102 50-51 0.4156239547860352 1102 52-53 0.4202392356825513 1102 54-55 0.4846176502015709 1102 56-57 0.409816381257464 1102 58-59 0.36334948848587345 1102 60-61 0.4314098873867849 1102 62-63 0.375199179998269 1102 64-65 0.407977730163644 1102 66-67 0.38117220987194145 1102 68-69 0.33245005071538003 1102 70-71 0.31432002867132525 1102 72-73 0.22503331691817863 1102 74-75 0.24244662326245958 1102 76-77 0.20250434126844397 1102 78-79 0.3299134855635302 1102 80-81 0.28383535881493316 1102 82-83 0.34455036291726415 1102 84-85 0.3447562562707738 1102 86-87 0.28747219649455147 1102 88-89 0.1854789265590746 1102 90-91 0.09997905721754563 1102 92-93 0.15094070665256965 1102 94-95 0.14153548537553462 1102 96-97 0.12150489930215969 1102 98-99 0.11065964226925118 1102 100-101 0.06133184274789372 1103 1 0.3218759766578856 1103 2 0.3546703599832597 1103 3 0.2987719050857578 1103 4 0.2584837754391174 1103 5 0.25286824086641246 1103 6 0.2577730373674143 1103 7 0.2650810251949167 1103 8 0.27183382042335325 1103 9 0.3140574278251975 1103 10-11 0.34146142960641157 1103 12-13 0.3368419641070872 1103 14-15 0.3867995074239161 1103 16-17 0.40013788219319935 1103 18-19 0.3754671633669773 1103 20-21 0.3261387896877821 1103 22-23 0.287426128974154 1103 24-25 0.3182813122492476 1103 26-27 0.27330014042217243 1103 28-29 0.307859017997842 1103 30-31 0.30625611339893055 1103 32-33 0.3562890968717909 1103 34-35 0.3405803698577188 1103 36-37 0.3286518335044164 1103 38-39 0.4167684192771901 1103 40-41 0.39777123011359805 1103 42-43 0.5014445758136787 1103 44-45 0.5079760362462409 1103 46-47 0.5980708643043329 1103 48-49 0.5346690807605157 1103 50-51 0.6132327275078424 1103 52-53 0.5661761331403241 1103 54-55 0.5749712497033101 1103 56-57 0.5325514173457719 1103 58-59 0.5094187275398028 1103 60-61 0.5915352006527073 1103 62-63 0.5817665562687964 1103 64-65 0.5985294368443093 1103 66-67 0.5010864559662664 1103 68-69 0.4863495842177201 1103 70-71 0.4543597156312913 1103 72-73 0.4873964619131854 1103 74-75 0.5033583042576595 1103 76-77 0.5096383033809886 1103 78-79 0.5352505591778751 1103 80-81 0.48587997181361686 1103 82-83 0.48200387954816293 1103 84-85 0.4749049737384503 1103 86-87 0.5037597914227021 1103 88-89 0.4527084574094111 1103 90-91 0.5019189522340177 1103 92-93 0.47716067200752477 1103 94-95 0.5092289618918429 1103 96-97 0.4853985339041671 1103 98-99 0.43059274380793156 1103 100-101 0.42657987072879067 1104 1 0.2528090119479529 1104 2 0.2790258945897932 1104 3 0.2677825407158565 1104 4 0.19768305173737133 1104 5 0.17153693977101625 1104 6 0.2666313169016732 1104 7 0.2065116294495084 1104 8 0.20996037423289238 1104 9 0.23511987248668476 1104 10-11 0.2628229381142688 1104 12-13 0.200633189051878 1104 14-15 0.21042408474141183 1104 16-17 0.3010434356161156 1104 18-19 0.2575290786997755 1104 20-21 0.21892232101337328 1104 22-23 0.23410955714411585 1104 24-25 0.24782282715845128 1104 26-27 0.24193619796290733 1104 28-29 0.2104477971902483 1104 30-31 0.16668836533057174 1104 32-33 0.23108930359471458 1104 34-35 0.2878185982978678 1104 36-37 0.3096941736243437 1104 38-39 0.32780104588983505 1104 40-41 0.35996042103658965 1104 42-43 0.3425381060998234 1104 44-45 0.40018309501407856 1104 46-47 0.45129642453339613 1104 48-49 0.3419555319701644 1104 50-51 0.36760405449677336 1104 52-53 0.32262706492004156 1104 54-55 0.2805676912972288 1104 56-57 0.2188804577308403 1104 58-59 0.2567217584353685 1104 60-61 0.28021077431224484 1104 62-63 0.3107585110216533 1104 64-65 0.26656729808198065 1104 66-67 0.3084348279975444 1104 68-69 0.2672056558715994 1104 70-71 0.2560796050451657 1104 72-73 0.23544044147691778 1104 74-75 0.27442183810810405 1104 76-77 0.28127390157557386 1104 78-79 0.411511496950709 1104 80-81 0.2914580772971931 1104 82-83 0.31924954534793315 1104 84-85 0.3180800041757088 1104 86-87 0.25740338580804334 1104 88-89 0.2320365084335414 1104 90-91 0.20571142245627527 1104 92-93 0.17012833630267465 1104 94-95 0.10385094127924077 1104 96-97 0.04723039365916648 1104 98-99 0.1406229306372886 1104 100-101 0.1879109008133888 1105 1 0.3302960142668283 1105 2 0.3065409357108564 1105 3 0.28389911863035877 1105 4 0.18974245077156127 1105 5 0.21930056074489812 1105 6 0.22897000874096562 1105 7 0.2603292385010576 1105 8 0.28180691701295046 1105 9 0.3292439472624835 1105 10-11 0.23270326634350624 1105 12-13 0.2216370639799905 1105 14-15 0.3468888749095669 1105 16-17 0.32687803574342666 1105 18-19 0.3478375559603464 1105 20-21 0.28280536330185413 1105 22-23 0.2290503698247548 1105 24-25 0.286221151393832 1105 26-27 0.32716299457837295 1105 28-29 0.3102798961071258 1105 30-31 0.3746003613529254 1105 32-33 0.35494112636565234 1105 34-35 0.38237121408801755 1105 36-37 0.3075260725892619 1105 38-39 0.35135598384083266 1105 40-41 0.38799734455012214 1105 42-43 0.42692053982452194 1105 44-45 0.43192833692675237 1105 46-47 0.4102971677609446 1105 48-49 0.40274819609270907 1105 50-51 0.36515359367724187 1105 52-53 0.3582212127490578 1105 54-55 0.3633608759312352 1105 56-57 0.3648564550673896 1105 58-59 0.31796632007303316 1105 60-61 0.3619229676225544 1105 62-63 0.33415859367997314 1105 64-65 0.3103454960849561 1105 66-67 0.3750791756659595 1105 68-69 0.31865893543150037 1105 70-71 0.24239090999943613 1105 72-73 0.30334085267487865 1105 74-75 0.3363464208810356 1105 76-77 0.3908944730201256 1105 78-79 0.4575708126800464 1105 80-81 0.39681735672409957 1105 82-83 0.48115867051784633 1105 84-85 0.46532070525458735 1105 86-87 0.46191534385408417 1105 88-89 0.461825548625729 1105 90-91 0.48177724771007746 1105 92-93 0.509407515422545 1105 94-95 0.5159240062358883 1105 96-97 0.5039400841850252 1105 98-99 0.49084034834831414 1105 100-101 0.514737409216405 1106 1 0.22357252102405312 1106 2 0.17591132063751758 1106 3 0.20047023449314594 1106 4 0.09616366125140985 1106 5 0.19969948375628377 1106 6 0.18127784221778143 1106 7 0.21888851811139176 1106 8 0.18709777652781412 1106 9 0.2568526925227843 1106 10-11 0.2058851977048377 1106 12-13 0.1763792539613931 1106 14-15 0.2547615876560272 1106 16-17 0.2098833490466987 1106 18-19 0.21219019843136522 1106 20-21 0.19202387148043698 1106 22-23 0.21898850689621696 1106 24-25 0.26005656320202064 1106 26-27 0.2621344329863078 1106 28-29 0.337848963409904 1106 30-31 0.32140122695158624 1106 32-33 0.3019057050325884 1106 34-35 0.33981091410991837 1106 36-37 0.2812269099350786 1106 38-39 0.39368498713724875 1106 40-41 0.391677717296659 1106 42-43 0.45042790902114405 1106 44-45 0.4431943330560344 1106 46-47 0.4822790509152952 1106 48-49 0.38009961553827054 1106 50-51 0.4012708745252098 1106 52-53 0.43411013330097603 1106 54-55 0.38432397316129396 1106 56-57 0.307159051555125 1106 58-59 0.4219248636189832 1106 60-61 0.5244789258552274 1106 62-63 0.4985852317819379 1106 64-65 0.5699645893863803 1106 66-67 0.46801599283732287 1106 68-69 0.42201707635152275 1106 70-71 0.46739202693698445 1106 72-73 0.4609648245292064 1106 74-75 0.45648066745999216 1106 76-77 0.3749932622598138 1106 78-79 0.3710125726769107 1106 80-81 0.41766252770428736 1106 82-83 0.36732922733941464 1106 84-85 0.4172527938255186 1106 86-87 0.41328609297520913 1106 88-89 0.4293398306072902 1106 90-91 0.42912057744538856 1106 92-93 0.4562549214972407 1106 94-95 0.4967859544818616 1106 96-97 0.4610045897251531 1106 98-99 0.4986650674990649 1106 100-101 0.5025912510531008 1107 1 0.23878721917247958 1107 2 0.2484942111206081 1107 3 0.207135591839112 1107 4 0.17718363918780966 1107 5 0.18662696328728856 1107 6 0.15815826026037882 1107 7 0.17152861452058232 1107 8 0.14798823901523406 1107 9 0.22792659584245456 1107 10-11 0.16425929919667936 1107 12-13 0.16982113880127514 1107 14-15 0.20712365090187035 1107 16-17 0.22500007031282365 1107 18-19 0.17063118949036493 1107 20-21 0.11697893640388202 1107 22-23 0.2030555502377922 1107 24-25 0.19526748808436878 1107 26-27 0.19222286282082024 1107 28-29 0.24550810108761567 1107 30-31 0.26969255521662205 1107 32-33 0.2741868214519698 1107 34-35 0.19812167910571787 1107 36-37 0.17553017013373307 1107 38-39 0.26585809447353626 1107 40-41 0.24013607446387653 1107 42-43 0.1941836210478698 1107 44-45 0.13473725361202327 1107 46-47 0.17741589714485428 1107 48-49 0.11350806530420954 1107 50-51 0.16214913083918248 1107 52-53 0.06846753446989595 1107 54-55 0.06373540224072372 1107 56-57 0.13093552872345526 1107 58-59 0.13810462953232872 1107 60-61 0.12241766077166716 1107 62-63 0.13410074652452408 1107 64-65 0.1742943348584589 1107 66-67 0.12312501616669635 1107 68-69 0.09102938709288111 1107 70-71 0.10068888821748345 1107 72-73 0.16077685571183764 1107 74-75 0.185098659060138 1107 76-77 0.17745527887633727 1107 78-79 0.25950652045884226 1107 80-81 0.2748132701118351 1107 82-83 0.29809908486800296 1107 84-85 0.31611445171845176 1107 86-87 0.29309288062139416 1107 88-89 0.25614546135772187 1107 90-91 0.2925054538434395 1107 92-93 0.31678276008097583 1107 94-95 0.4273236527967974 1107 96-97 0.4597238137357387 1107 98-99 0.49014125976671963 1107 100-101 0.5470570967579214 1108 1 0.12383224199682275 1108 2 0.15837652115784095 1108 3 0.14587907485863383 1108 4 0.144912825473277 1108 5 0.12842390381538138 1108 6 0.15536151829194722 1108 7 0.1555269661810783 1108 8 0.15515230734305163 1108 9 0.24311301305907307 1108 10-11 0.18181630025322448 1108 12-13 0.14255516666136714 1108 14-15 0.1993197035533072 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63.0 5 133.0 6 389.0 7 1129.0 8 2912.0 9 6216.0 10 10537.0 11 16120.0 12 23616.0 13 32924.0 14 38354.0 15 45601.0 16 55431.0 17 72741.0 18 62145.0 19 36015.0 20 28068.0 21 26709.0 22 27382.0 23 29916.0 24 35305.0 25 44265.0 26 55117.0 27 69679.0 28 88867.0 29 113017.0 30 142288.0 31 180856.0 32 230206.0 33 304699.0 34 422798.0 35 625017.0 36 982316.0 37 1554910.0 38 1712652.0 39 447497.0 40 213.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.74578830776168 21.943751402479425 15.670879688269371 22.639580601489527 2 14.994764801394636 21.97372302344678 39.25867461779064 23.772837557367943 3 16.224665713983907 27.28389082547902 31.95460074686608 24.536842713670996 4 12.023763129785587 19.275248724009327 39.03946167164909 29.661526474555988 5 11.904621121897947 37.15118490799093 35.14719683633001 15.79699713378111 6 27.941019954416706 39.47810012831513 18.64272443154753 13.93815548572064 7 24.95851457394411 33.36378277461598 23.51369633584932 18.16400631559059 8 24.699885837832323 34.6924238413975 21.681364780014604 18.926325540755577 9 24.198396653793747 17.23088942730897 23.120021099807538 35.450692819089745 10-11 22.724202517453676 27.349769699234127 29.334254035918693 20.5917737473935 12-13 24.318166145527247 24.899659679328188 29.549794270287666 21.232379904856902 14-15 21.584594100745885 26.87340621389234 27.876920116968883 23.665079568392887 16-17 20.00302412778295 30.11861197849574 28.116077344858326 21.76228654886298 18-19 19.78901514157504 27.8185841134208 31.885865682008657 20.506535062995503 20-21 21.222874301439 27.379412421831155 31.390681743185194 20.007031533544648 22-23 20.67597228166322 27.58530040045776 31.458429470523857 20.280297847355158 24-25 20.43903774540198 27.401539084907974 31.59872871663667 20.56069445305338 26-27 20.27790233421457 27.2697429113066 31.655476740804563 20.79687801367427 28-29 20.47088844961813 27.32196644947037 31.621382150854604 20.5857629500569 30-31 20.92973583464129 27.95233231188287 31.04587558235133 20.07205627112451 32-33 20.135779164556116 27.57816042040609 31.747234401268404 20.538826013769395 34-35 20.263291719204222 27.110534764879574 31.68681996005883 20.93935355585737 36-37 21.165613602145044 27.796824077345534 30.703207746458755 20.33435457405067 38-39 20.82815486849218 27.90695325958105 30.927478416539444 20.337413455387317 40-41 20.442062360641387 27.675987646553672 31.121398267897266 20.76055172490768 42-43 20.421803246047773 27.71732216039913 30.814726115424907 21.046148478128185 44-45 20.870560249489625 28.012946174069665 30.501524398244534 20.61496917819618 46-47 20.849108166497732 28.640185519810966 29.717563414181576 20.793142899509725 48-49 20.785530346823016 28.162092525955572 30.02197519514222 21.030401932079194 50-51 20.905090671636263 27.991781324829866 29.745946418899045 21.357181584634823 52-53 20.896190620832353 28.271272952335625 29.474950561236103 21.357585865595922 54-55 21.152142844235346 28.969447807568145 29.155102422618334 20.72330692557818 56-57 20.712603761992572 28.53144210601003 29.236267621634422 21.51968651036298 58-59 20.522593712845843 28.037638737636982 29.730718903184076 21.709048646333095 60-61 21.132179117154646 28.61443099541296 29.094635012192626 21.158754875239772 62-63 20.943969891779314 28.553207728279794 29.206868849753608 21.29595353018728 64-65 20.84565221406345 28.240674489363542 29.58281961757092 21.33085367900209 66-67 20.78697710993885 28.40941019237527 29.174239295080007 21.62937340260587 68-69 21.408471964961777 28.67452131285845 28.84940415789578 21.067602564283998 70-71 21.503418103504977 28.230655583070696 28.999056199763036 21.266870113661295 72-73 21.023514269927297 28.521591458618715 29.35968274095066 21.095211530503324 74-75 20.975080394450522 28.555508229855146 29.12669200728714 21.34271936840719 76-77 21.104977984256664 28.85203756907076 28.72951528691686 21.313469159755716 78-79 21.250422039910724 28.896104370652626 28.918267694417725 20.935205895018928 80-81 21.033722940838313 28.55507021352488 29.084283402039983 21.326923443596822 82-83 20.796366256329204 28.646172049418546 29.203318176125887 21.354143518126367 84-85 20.924931619553124 28.715688614734486 28.941018056982166 21.41836170873022 86-87 21.097013334314855 28.8374216280712 28.849360663648266 21.216204373965674 88-89 20.61561759375505 28.798998186874503 29.008486564727438 21.57689765464301 90-91 20.440390816288907 28.676459108488956 29.014046891178197 21.86910318404394 92-93 20.78931280609195 28.925912643804008 28.705068701320425 21.579705848783618 94-95 20.651247426454223 28.631514749680942 28.980553660297936 21.736684163566895 96-97 20.51499686956226 28.76177368795329 29.02102468953392 21.70220475295053 98-99 20.409710126816652 28.729409243284852 28.987670442658793 21.873210187239703 100-101 20.47352854595828 28.961178017589507 28.682362947092834 21.882930489359378 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2085.0 1 1540.5 2 2553.5 3 3555.5 4 2242.5 5 1588.0 6 2543.0 7 5204.0 8 8717.5 9 10556.0 10 9260.0 11 7856.0 12 8259.0 13 9124.5 14 10087.0 15 10961.5 16 11798.0 17 12770.0 18 13877.5 19 15303.5 20 17370.5 21 20672.5 22 26424.0 23 35583.5 24 46186.5 25 58170.0 26 75421.5 27 96586.0 28 122050.5 29 146696.0 30 164810.5 31 182180.0 32 200095.0 33 216900.0 34 231621.5 35 242803.0 36 248940.0 37 252395.0 38 256816.5 39 256879.5 40 254253.5 41 251390.5 42 244368.0 43 236428.0 44 230699.0 45 226130.0 46 221527.0 47 217607.5 48 215769.5 49 217089.5 50 215299.5 51 205805.5 52 190968.0 53 177214.0 54 168421.0 55 161636.0 56 151447.0 57 138500.0 58 126697.5 59 114979.5 60 101116.0 61 85317.5 62 70642.0 63 57084.5 64 45203.0 65 35891.5 66 28360.0 67 21881.0 68 16459.5 69 12249.5 70 9049.5 71 6780.0 72 5143.0 73 4095.5 74 3332.5 75 2580.5 76 1943.5 77 1484.0 78 1148.5 79 879.0 80 632.5 81 413.0 82 260.5 83 155.0 84 105.5 85 77.5 86 52.0 87 38.5 88 25.5 89 17.0 90 14.0 91 9.5 92 6.5 93 5.5 94 4.5 95 2.5 96 2.0 97 0.5 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04975992331789868 2 0.0020860635409639767 3 2.9231463631342345E-4 4 3.1888869416009835E-4 5 0.0025776836111274615 6 0.0017804618757272157 7 0.0 8 1.3287028923337429E-5 9 2.6574057846674858E-5 10-11 6.6435144616687146E-6 12-13 0.0 14-15 4.384719544701352E-4 16-17 0.0 18-19 5.979163015501844E-5 20-21 5.381246713951659E-4 22-23 0.0 24-25 5.514117003185034E-4 26-27 0.007221500219833894 28-29 0.00708198641613885 30-31 9.898836547886387E-4 32-33 0.006125320333658556 34-35 0.007547032428455661 36-37 0.004304997371161327 38-39 0.002358447633892394 40-41 0.0018601840492672402 42-43 0.0012290501754087125 44-45 6.97569018475215E-4 46-47 5.381246713951659E-4 48-49 0.0020129848818856207 50-51 0.0014150685803354364 52-53 0.002125924627733989 54-55 8.63656880016933E-4 56-57 2.524535495434112E-4 58-59 8.105087643235833E-4 60-61 0.006397704426586973 62-63 0.0029098593342108976 64-65 0.0011692585452536938 66-67 0.005361316170566653 68-69 0.007008907757060494 70-71 0.0023916652062007373 72-73 6.311338738585279E-4 74-75 0.0036605764683794624 76-77 0.0021126375988106513 78-79 0.0025245354954341118 80-81 7.772911920152397E-4 82-83 1.9266191938839276E-4 84-85 6.045598160118531E-4 86-87 0.005361316170566653 88-89 0.002424882778509081 90-91 0.0012556242332553871 92-93 1.0629623138669943E-4 94-95 5.580552147801721E-4 96-97 4.384719544701352E-4 98-99 4.716895267784788E-4 100-101 1.9930543385006147E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7526137.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.168075836976286 #Duplication Level Percentage of deduplicated Percentage of total 1 72.56077067044922 40.75598869807236 2 16.437393247940168 18.4651350102501 3 5.2160147497841685 8.789205360979922 4 2.0691891281642536 4.648894874871065 5 1.0517878665311384 2.9538450325866235 6 0.628630081698641 2.1185365261352316 7 0.39617090422338924 1.5576510174975957 8 0.28011929384726064 1.2587009392170543 9 0.2006500247995795 1.0143113228670557 >10 1.0623660425326455 10.620520948434327 >50 0.06510714146952269 2.5061353677940006 >100 0.0290755087121083 2.862056691875851 >500 0.0018723045277344197 0.6951233586365317 >1k 7.345581960563638E-4 0.6671668112851278 >5k 4.7390849659352985E-5 0.14295480664573976 >10k+ 7.108627448902948E-5 0.9437732328513223 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30299 0.4025836893482009 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24618 0.32710007803472085 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15994 0.21251274059985886 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 1.328702892333743E-5 0.0 7 0.0 0.0 0.0 1.328702892333743E-5 0.0 8 0.0 0.0 0.0 1.328702892333743E-5 0.0 9 1.328702892333743E-5 0.0 0.0 1.328702892333743E-5 0.0 10-11 1.9930543385006147E-5 6.643514461668715E-6 0.0 1.328702892333743E-5 0.0 12-13 3.3217572308343576E-5 1.9930543385006147E-5 0.0 1.9930543385006147E-5 0.0 14-15 3.9861086770012294E-5 2.657405784667486E-5 0.0 7.307865907835588E-5 0.0 16-17 3.9861086770012294E-5 2.657405784667486E-5 0.0 7.972217354002459E-5 0.0 18-19 3.9861086770012294E-5 3.3217572308343576E-5 0.0 7.972217354002459E-5 0.0 20-21 3.9861086770012294E-5 5.314811569334972E-5 0.0 7.972217354002459E-5 0.0 22-23 5.314811569334972E-5 6.643514461668715E-5 0.0 9.965271692503072E-5 0.0 24-25 5.314811569334972E-5 6.643514461668715E-5 0.0 2.3916652062007376E-4 0.0 26-27 5.314811569334972E-5 6.643514461668715E-5 0.0 4.650460123168101E-4 0.0 28-29 5.314811569334972E-5 6.643514461668715E-5 0.0 8.237957932469207E-4 0.0 30-31 5.314811569334972E-5 6.643514461668715E-5 0.0 0.0019199756794222587 0.0 32-33 5.314811569334972E-5 7.972217354002459E-5 0.0 0.004192057625312959 0.0 34-35 5.314811569334972E-5 9.300920246336201E-5 0.0 0.008417332822934262 0.0 36-37 5.314811569334972E-5 9.965271692503072E-5 0.0 0.0145891577578245 0.0 38-39 5.314811569334972E-5 1.1958326031003687E-4 0.0 0.024282045357399154 0.0 40-41 5.314811569334972E-5 1.1958326031003687E-4 0.0 0.03804740732197673 0.0 42-43 5.314811569334972E-5 1.1958326031003687E-4 0.0 0.05282922699918963 0.0 44-45 5.314811569334972E-5 1.1958326031003687E-4 0.0 0.0693317169219747 0.0 46-47 5.314811569334972E-5 1.4615731815671173E-4 0.0 0.08877064023681738 0.0 48-49 5.979163015501844E-5 1.4615731815671173E-4 0.0 0.11190335759234785 0.0 50-51 6.643514461668715E-5 1.4615731815671173E-4 0.0 0.13823824891840264 0.0 52-53 6.643514461668715E-5 1.4615731815671173E-4 0.0 0.16495182056877253 0.0 54-55 6.643514461668715E-5 1.4615731815671173E-4 0.0 0.19439587666288827 0.0 56-57 7.972217354002459E-5 1.5944434708004918E-4 0.0 0.2289620824069506 0.0 58-59 7.972217354002459E-5 1.5944434708004918E-4 0.0 0.26772034577632586 0.0 60-61 7.972217354002459E-5 1.727313760033866E-4 0.0 0.3097405747463805 0.0 62-63 7.972217354002459E-5 1.727313760033866E-4 0.0 0.35451786221802767 0.0 64-65 7.972217354002459E-5 1.793748904650553E-4 0.0 0.4080380147212308 0.0 66-67 7.972217354002459E-5 2.0594894831173018E-4 0.0 0.4702877452270667 0.0 68-69 8.63656880016933E-5 2.192359772350676E-4 0.0 0.5369288387920655 0.0 70-71 9.300920246336201E-5 2.3916652062007376E-4 0.0 0.612724695285244 0.0 72-73 9.300920246336201E-5 2.524535495434112E-4 0.0 0.7023789229454633 0.0 74-75 1.0629623138669943E-4 2.524535495434112E-4 0.0 0.7961654166008405 0.0 76-77 1.1958326031003687E-4 2.9231463631342345E-4 0.0 0.8933932507473621 0.0 78-79 1.1958326031003687E-4 2.9231463631342345E-4 0.0 1.004771770697238 0.0 80-81 1.1958326031003687E-4 2.9231463631342345E-4 0.0 1.12974956475015 0.0 82-83 1.1958326031003687E-4 2.9231463631342345E-4 0.0 1.2685591559122562 0.0 84-85 1.1958326031003687E-4 2.9895815077509217E-4 0.0 1.4114943695550586 0.0 86-87 1.1958326031003687E-4 3.0560166523676093E-4 0.0 1.5682613803070553 1.328702892333743E-5 88-89 1.1958326031003687E-4 3.0560166523676093E-4 0.0 1.7481345343567356 1.328702892333743E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14760 0.0 55.18711 1 TATAACG 1110 0.0 44.075428 2 ATAACGC 1065 0.0 43.706913 3 GTATCAA 28020 0.0 42.317074 1 ATCAACG 34925 0.0 33.319874 3 TCAACGC 35250 0.0 32.972244 4 CAACGCA 36360 0.0 32.200592 5 TATCAAC 36230 0.0 32.185886 2 AACGCAG 38395 0.0 30.728336 6 TAACGCA 1785 0.0 29.802551 4 GTACCTG 5880 0.0 28.368933 1 TACCTGG 5770 0.0 28.071194 2 TCTGTCG 2070 0.0 27.304241 8 ACGCAGA 43375 0.0 27.18884 7 CGCAGAG 43425 0.0 27.0591 8 GCAGAGT 46640 0.0 24.817398 9 GTATAAC 2775 0.0 23.633568 1 GTGGTAT 6790 0.0 23.37706 1 TGGTATC 6825 0.0 22.61846 2 AGAGTAC 43290 0.0 22.585125 10-11 >>END_MODULE