FastQCFastQC Report
Wed 25 May 2016
SRR2049397_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049397_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8837457
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT391670.44319310407960116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328730.37197352134216893No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173900.19677606352144061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA218600.054.1305731
GTATCAA407850.042.6517561
ATCAACG503150.034.164943
TCAACGC506550.033.993044
TATCAAC518400.033.241042
CAACGCA524650.032.8288045
AACGCAG547600.031.4964926
CGCAGAG603350.028.248948
ACGCAGA609750.028.0615047
GCAGAGT640850.025.9139779
GTACATG385000.023.998161
GAGTACT394700.023.63725912-13
TACATGG377350.023.3617822
GTGGTAT101450.022.943681
TGGTATC95400.022.8028682
AGAGTAC585000.022.11118710-11
CAGAGTA635550.021.90717510-11
ACATGGG388400.021.7441313
GTACTTT430400.021.48185214-15
AGTACTT410000.021.30691712-13
Produced by FastQC (version 0.11.5)