FastQCFastQC Report
Wed 25 May 2016
SRR2049397_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049397_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8837457
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT375720.4251449257405156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316890.35857600212368784No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198200.22427266124180295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA213650.053.4759181
GTATCAA392600.040.92811
ATCAACG504500.031.5677363
TCAACGC510150.031.19954
TATCAAC523450.030.5168692
CAACGCA529500.030.077295
AACGCAG556350.028.6846796
CGCAGAG618800.025.612968
ACGCAGA626500.025.3965767
GTGGTAT101500.023.6456931
GCAGAGT664150.023.5280579
TGGTATC100300.022.9683112
GTACATG365650.022.1088121
AGAGTAC604550.021.99401710-11
GAGTACT415350.021.98980712-13
TACATGG359850.021.6344432
AGTACTT434900.020.97399712-13
ACATGGG365250.020.442473
CAGAGTA656600.020.23965810-11
GTACTTT449150.020.13425414-15
Produced by FastQC (version 0.11.5)