##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049397_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8837457 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06312109920309 33.0 31.0 34.0 30.0 34.0 2 32.250133381129885 34.0 31.0 34.0 30.0 34.0 3 32.31536888948936 34.0 31.0 34.0 30.0 34.0 4 35.95596244485263 37.0 35.0 37.0 35.0 37.0 5 35.84658889995165 37.0 35.0 37.0 35.0 37.0 6 35.876402227473356 37.0 35.0 37.0 35.0 37.0 7 35.803995538535574 37.0 35.0 37.0 35.0 37.0 8 35.794394020813904 37.0 35.0 37.0 35.0 37.0 9 37.39857483889313 39.0 37.0 39.0 34.0 39.0 10-11 37.393570175221214 39.0 37.0 39.0 34.0 39.0 12-13 37.37593235248556 39.0 37.0 39.0 34.0 39.0 14-15 38.585717531638345 40.0 38.0 41.0 34.0 41.0 16-17 38.584992323017815 40.0 38.0 41.0 34.0 41.0 18-19 38.6454911746671 40.0 38.0 41.0 34.0 41.0 20-21 38.649564914431835 40.0 38.0 41.0 34.0 41.0 22-23 38.56726114763557 40.0 38.0 41.0 34.0 41.0 24-25 38.47207799709803 40.0 38.0 41.0 34.0 41.0 26-27 38.297741024369344 40.0 38.0 41.0 34.0 41.0 28-29 38.11579366100452 40.0 38.0 41.0 33.5 41.0 30-31 37.92020821147984 40.0 37.0 41.0 33.0 41.0 32-33 37.65752857411357 40.0 37.0 41.0 33.0 41.0 34-35 37.46657550922171 40.0 36.0 41.0 32.5 41.0 36-37 37.269103261266224 39.5 36.0 41.0 32.0 41.0 38-39 37.39803243172781 40.0 37.0 41.0 33.0 41.0 40-41 37.29038494897344 40.0 37.0 41.0 32.0 41.0 42-43 37.086665824795524 40.0 36.0 41.0 31.5 41.0 44-45 36.81928692835507 40.0 36.0 41.0 31.0 41.0 46-47 36.44989423993803 40.0 36.0 41.0 30.0 41.0 48-49 36.14224980104571 39.0 36.0 41.0 29.5 41.0 50-51 35.907080000502404 39.0 35.0 41.0 29.0 41.0 52-53 35.73047071120119 39.0 35.0 40.5 28.0 41.0 54-55 35.635587250947864 39.0 35.0 40.0 28.0 41.0 56-57 35.55143034925092 39.0 35.0 40.5 28.0 41.0 58-59 35.331801897310505 39.0 35.0 40.0 27.5 41.0 60-61 35.10559412057111 38.0 35.0 40.0 27.0 41.0 62-63 34.846644741807516 38.0 34.0 40.0 26.0 41.0 64-65 34.6138657874092 38.0 34.0 40.0 26.0 41.0 66-67 34.298533899514304 37.0 34.0 40.0 26.0 41.0 68-69 33.99757741395517 37.0 34.0 40.0 25.5 41.0 70-71 33.67946282510908 36.0 33.5 39.0 25.0 41.0 72-73 33.3270854387184 36.0 33.0 39.0 24.5 40.5 74-75 32.96065140684702 35.5 33.0 39.0 23.5 40.0 76-77 30.939661884634912 33.5 30.0 36.0 21.0 38.5 78-79 32.254584095854725 35.0 32.5 37.0 23.0 39.0 80-81 32.263776842139094 35.0 33.0 37.0 23.5 39.0 82-83 31.9808663849793 35.0 33.0 36.5 23.0 39.0 84-85 31.700527029438447 35.0 32.5 36.0 22.0 37.5 86-87 31.419932849461105 35.0 32.0 36.0 20.5 37.0 88-89 31.14208272809701 35.0 32.0 35.0 20.0 37.0 90-91 30.868207053228097 35.0 32.0 35.0 18.5 36.0 92-93 30.62052194426519 35.0 32.0 35.0 17.5 36.0 94-95 30.36013691495189 34.0 31.5 35.0 11.5 36.0 96-97 30.121870974874334 34.0 31.0 35.0 4.5 35.5 98-99 29.84419856300291 34.0 31.0 35.0 2.0 35.0 100-101 29.24424690270063 34.0 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37423592756623947 1101 2 0.39843472062658947 1101 3 0.3629917646197498 1101 4 0.2642960804924286 1101 5 0.31288620435033465 1101 6 0.33111587232166073 1101 7 0.3514877104160874 1101 8 0.3728569498221148 1101 9 0.42991726173997336 1101 10-11 0.4130380930897033 1101 12-13 0.36556873673544743 1101 14-15 0.4484895881172264 1101 16-17 0.4096974361942429 1101 18-19 0.42380564058938575 1101 20-21 0.41847202040496967 1101 22-23 0.36943589293641566 1101 24-25 0.31846109453395144 1101 26-27 0.3113265446908926 1101 28-29 0.34291066679322313 1101 30-31 0.3400667447532726 1101 32-33 0.26867686542266256 1101 34-35 0.2929804142434662 1101 36-37 0.24214626640698356 1101 38-39 0.33965392847250087 1101 40-41 0.3527515670173216 1101 42-43 0.2982316422492133 1101 44-45 0.2861544657368711 1101 46-47 0.3580359141606735 1101 48-49 0.2325283459883707 1101 50-51 0.33098935580073174 1101 52-53 0.26270102612458146 1101 54-55 0.2272405036565246 1101 56-57 0.17729001203910144 1101 58-59 0.17976297234923777 1101 60-61 0.18042037159043645 1101 62-63 0.16422392061753754 1101 64-65 0.07568573901600928 1101 66-67 0.0661537284313809 1101 68-69 -0.018121616016095743 1101 70-71 0.010223936040979709 1101 72-73 0.0648916365825869 1101 74-75 -0.016595969707061897 1101 76-77 -0.015236049809026042 1101 78-79 0.11385344809365705 1101 80-81 0.11018595383895047 1101 82-83 0.023706747855783306 1101 84-85 -0.02863136911480524 1101 86-87 -0.027732653636245885 1101 88-89 -0.12420848146416219 1101 90-91 -0.13025154118816218 1101 92-93 -0.08664052027512525 1101 94-95 -0.128707112482342 1101 96-97 -0.20671314487962533 1101 98-99 -0.2892532345834695 1101 100-101 -0.3438682875392054 1102 1 0.32486130033102256 1102 2 0.3053483763450302 1102 3 0.29869958560208687 1102 4 0.25735184255649557 1102 5 0.25732788632029724 1102 6 0.2680465486745405 1102 7 0.27556925129619003 1102 8 0.27914514881820907 1102 9 0.3740029907480533 1102 10-11 0.33324835098077443 1102 12-13 0.3192196284269997 1102 14-15 0.35755587499239994 1102 16-17 0.3758862651916033 1102 18-19 0.3568717370163128 1102 20-21 0.33868275225000133 1102 22-23 0.3090264936765692 1102 24-25 0.2758594995245147 1102 26-27 0.3290080876076402 1102 28-29 0.3606271468872393 1102 30-31 0.26503967114920357 1102 32-33 0.3054435726651974 1102 34-35 0.22373549764338918 1102 36-37 0.30124558922698697 1102 38-39 0.4539065553555304 1102 40-41 0.425323775798212 1102 42-43 0.482519307600235 1102 44-45 0.42552451268106495 1102 46-47 0.3713879824876116 1102 48-49 0.2042400927177681 1102 50-51 0.22755141026707548 1102 52-53 0.3216156287759375 1102 54-55 0.3087868139141534 1102 56-57 0.274114038460489 1102 58-59 0.24898190273103893 1102 60-61 0.2829735669636264 1102 62-63 0.2978565247280258 1102 64-65 0.30155083904548263 1102 66-67 0.26524767471772037 1102 68-69 0.2050580609033048 1102 70-71 0.19018113826965077 1102 72-73 0.08772501836755708 1102 74-75 0.15091505270153505 1102 76-77 0.19753034413641046 1102 78-79 0.22166879173275333 1102 80-81 0.22020216360588307 1102 82-83 0.2743262192167535 1102 84-85 0.2891277560019425 1102 86-87 0.16221059592965403 1102 88-89 0.09823119083269916 1102 90-91 0.020072705608274788 1102 92-93 -0.003874821281605989 1102 94-95 0.00806765739405435 1102 96-97 -0.018170632374417295 1102 98-99 -0.03463509495186656 1102 100-101 -0.10067000075004628 1103 1 0.3088301781907177 1103 2 0.3528059875006022 1103 3 0.3002182070965205 1103 4 0.22924186470073948 1103 5 0.2402553754090917 1103 6 0.2696608872921118 1103 7 0.22815802873277846 1103 8 0.27102129132916986 1103 9 0.3246778940305717 1103 10-11 0.3177741130922769 1103 12-13 0.24310787755398877 1103 14-15 0.3935206669095095 1103 16-17 0.4215838286379565 1103 18-19 0.3800851427002456 1103 20-21 0.33182115619207764 1103 22-23 0.2639940458580128 1103 24-25 0.27407880720065947 1103 26-27 0.2919891324951962 1103 28-29 0.2823188454418286 1103 30-31 0.33848830576095423 1103 32-33 0.3021128041032739 1103 34-35 0.27208846326374214 1103 36-37 0.2912809137626695 1103 38-39 0.36526051842167107 1103 40-41 0.35184232054202624 1103 42-43 0.4088980881729398 1103 44-45 0.4669460001579324 1103 46-47 0.5263014892273787 1103 48-49 0.5385278654810008 1103 50-51 0.5134012843986753 1103 52-53 0.4453818886539409 1103 54-55 0.41179453003075395 1103 56-57 0.39699497348799895 1103 58-59 0.4071802447408288 1103 60-61 0.4132058970597541 1103 62-63 0.4668234723747915 1103 64-65 0.41723265742833604 1103 66-67 0.4221959833605098 1103 68-69 0.43589715274423924 1103 70-71 0.4076016078512552 1103 72-73 0.4011937574777491 1103 74-75 0.4106425557563327 1103 76-77 0.38924103573646107 1103 78-79 0.5037384378947181 1103 80-81 0.477295975804779 1103 82-83 0.5556804065508061 1103 84-85 0.5208099598550078 1103 86-87 0.5079717010802334 1103 88-89 0.42788487852804735 1103 90-91 0.4691421551325696 1103 92-93 0.44764958122526366 1103 94-95 0.4555983091181304 1103 96-97 0.442563961032306 1103 98-99 0.45639381182746064 1103 100-101 0.4515916395474484 1104 1 0.3121900388473762 1104 2 0.3250702432251771 1104 3 0.2962556397625633 1104 4 0.23040856535025256 1104 5 0.25337712442313887 1104 6 0.25863550379644806 1104 7 0.2720555129083593 1104 8 0.2735832655722632 1104 9 0.3311940491274612 1104 10-11 0.2717469020056811 1104 12-13 0.23401115542566941 1104 14-15 0.323103891678862 1104 16-17 0.3959312338571763 1104 18-19 0.31805883088866693 1104 20-21 0.33516953157052143 1104 22-23 0.2948635785188003 1104 24-25 0.2953232758461368 1104 26-27 0.2785068475537571 1104 28-29 0.2988438510114051 1104 30-31 0.3380220989109617 1104 32-33 0.3227304565317439 1104 34-35 0.2844167736257148 1104 36-37 0.3517705144381935 1104 38-39 0.41210202618914593 1104 40-41 0.44152984639980986 1104 42-43 0.46377339215739255 1104 44-45 0.4753859234482434 1104 46-47 0.49941915520911806 1104 48-49 0.42121819461204524 1104 50-51 0.3506510679091335 1104 52-53 0.2924661309362193 1104 54-55 0.3254295456292198 1104 56-57 0.323271061708887 1104 58-59 0.29153450753015875 1104 60-61 0.30643157327030224 1104 62-63 0.3522854886571949 1104 64-65 0.42982902336668616 1104 66-67 0.465108183378085 1104 68-69 0.3920977356644215 1104 70-71 0.3207912428133497 1104 72-73 0.27777127793891765 1104 74-75 0.3513467813486315 1104 76-77 0.2969223694448928 1104 78-79 0.3825426642836689 1104 80-81 0.3330572853296019 1104 82-83 0.3949116391671481 1104 84-85 0.34230029999155676 1104 86-87 0.25825309994105083 1104 88-89 0.3406795408172165 1104 90-91 0.36299936893210116 1104 92-93 0.33362590253939217 1104 94-95 0.28058516881450757 1104 96-97 0.23941891389277714 1104 98-99 0.15653639726145485 1104 100-101 0.2817746745405536 1105 1 0.3131434205683803 1105 2 0.31180251208049725 1105 3 0.29675821038254213 1105 4 0.23574183115114522 1105 5 0.21468017352015778 1105 6 0.231133460862047 1105 7 0.2619535992049933 1105 8 0.23505236206792546 1105 9 0.29618643186803695 1105 10-11 0.2631813800673086 1105 12-13 0.23731377909791718 1105 14-15 0.267866480905127 1105 16-17 0.27029417298569314 1105 18-19 0.29027468870759066 1105 20-21 0.29439506385536873 1105 22-23 0.20617171228542475 1105 24-25 0.26291869611061003 1105 26-27 0.30816180769583923 1105 28-29 0.3147682665217175 1105 30-31 0.3586530101940326 1105 32-33 0.4061083742680154 1105 34-35 0.3645932121548583 1105 36-37 0.24274721680766476 1105 38-39 0.3424001180910068 1105 40-41 0.3911181904620591 1105 42-43 0.41887278042549525 1105 44-45 0.45352074623862393 1105 46-47 0.4504520783338748 1105 48-49 0.4489978096855296 1105 50-51 0.35761520263319113 1105 52-53 0.3574882618312074 1105 54-55 0.3559596191062724 1105 56-57 0.3640182064177182 1105 58-59 0.3701191064778939 1105 60-61 0.3919557902490638 1105 62-63 0.3720300860230239 1105 64-65 0.3765843910792128 1105 66-67 0.3918079155287444 1105 68-69 0.3500638377189489 1105 70-71 0.35159429964468103 1105 72-73 0.40705367809639625 1105 74-75 0.39533345393861197 1105 76-77 0.4421848919734188 1105 78-79 0.5024138862057654 1105 80-81 0.42701999385440104 1105 82-83 0.4903972814885904 1105 84-85 0.4302808429025333 1105 86-87 0.46495657688550196 1105 88-89 0.4861791752316904 1105 90-91 0.5332022699470542 1105 92-93 0.47530686619012386 1105 94-95 0.48045714171837517 1105 96-97 0.44653967420408947 1105 98-99 0.4388460125888045 1105 100-101 0.5049772444475416 1106 1 0.25175947100379403 1106 2 0.2207777097495054 1106 3 0.19723018434056883 1106 4 0.193980300437552 1106 5 0.21284637277778984 1106 6 0.23995863313029986 1106 7 0.258427133663119 1106 8 0.2359405767755689 1106 9 0.33307884690029255 1106 10-11 0.2501674689702682 1106 12-13 0.2560897726150344 1106 14-15 0.3142873564153703 1106 16-17 0.31611211054372745 1106 18-19 0.31749986164992094 1106 20-21 0.29056746080240714 1106 22-23 0.2602368090892 1106 24-25 0.25884199865102886 1106 26-27 0.3202433170112968 1106 28-29 0.38652689744309754 1106 30-31 0.35116500495244907 1106 32-33 0.3626572331894877 1106 34-35 0.37802596491761875 1106 36-37 0.43383483764400665 1106 38-39 0.44309959104553087 1106 40-41 0.3988331779864538 1106 42-43 0.44935972396297075 1106 44-45 0.48604859109624954 1106 46-47 0.5437097343020483 1106 48-49 0.4740988557097978 1106 50-51 0.49506661760713655 1106 52-53 0.447529982309824 1106 54-55 0.4491703433462959 1106 56-57 0.3948638756396292 1106 58-59 0.5040712979969086 1106 60-61 0.49202227655415953 1106 62-63 0.44883370734084593 1106 64-65 0.49039548605944105 1106 66-67 0.5154273451725047 1106 68-69 0.5297425815827879 1106 70-71 0.5379284767949954 1106 72-73 0.5367531363549389 1106 74-75 0.49367181178586605 1106 76-77 0.46466602616217756 1106 78-79 0.5634376850961402 1106 80-81 0.6253957806067447 1106 82-83 0.5514133978254492 1106 84-85 0.5466766648622752 1106 86-87 0.5624314776733819 1106 88-89 0.5788526411564803 1106 90-91 0.5372218439038612 1106 92-93 0.5422726369694395 1106 94-95 0.7030727602455933 1106 96-97 0.6632809644869724 1106 98-99 0.7549654199903273 1106 100-101 0.7289717512235185 1107 1 0.20495664209955322 1107 2 0.2048106281151334 1107 3 0.1575924747340025 1107 4 0.14083376271675974 1107 5 0.13285659782675197 1107 6 0.14842171418261074 1107 7 0.17931099992770072 1107 8 0.13910155647707256 1107 9 0.22267159668020753 1107 10-11 0.17202307039634235 1107 12-13 0.1557116026173091 1107 14-15 0.18063016087319284 1107 16-17 0.20593617307996936 1107 18-19 0.14252900737377416 1107 20-21 0.13535674740946746 1107 22-23 0.14132707100599617 1107 24-25 0.14494007399351005 1107 26-27 0.13927324544130926 1107 28-29 0.1698017652262891 1107 30-31 0.1914317552272493 1107 32-33 0.21772808509395247 1107 34-35 0.17688708653574992 1107 36-37 0.24170438518753912 1107 38-39 0.2729487082633284 1107 40-41 0.21119878733261288 1107 42-43 0.2905036157648553 1107 44-45 0.20013804965808646 1107 46-47 0.25028358585776544 1107 48-49 0.23551139294642098 1107 50-51 0.2709324278650769 1107 52-53 0.22413780380995973 1107 54-55 0.21761979108115526 1107 56-57 0.29379829925849776 1107 58-59 0.3565717890145166 1107 60-61 0.395520010568184 1107 62-63 0.3967737210849549 1107 64-65 0.49164073251473184 1107 66-67 0.4381910978414467 1107 68-69 0.4784052300623429 1107 70-71 0.4649540382486137 1107 72-73 0.5158398285141033 1107 74-75 0.4787154557389002 1107 76-77 0.47965295971255983 1107 78-79 0.5698712271235209 1107 80-81 0.625887662635705 1107 82-83 0.6324124191018399 1107 84-85 0.5782957574870693 1107 86-87 0.5737320292932431 1107 88-89 0.5719696226125492 1107 90-91 0.6537941348685479 1107 92-93 0.615606000496765 1107 94-95 0.622889548951985 1107 96-97 0.6644517608393627 1107 98-99 0.7813217243276398 1107 100-101 0.8615976028219059 1108 1 0.18006805586417585 1108 2 0.14214098797824448 1108 3 0.12204967814766832 1108 4 0.13126952300951444 1108 5 0.0741625780725812 1108 6 0.11841560527604855 1108 7 0.1348861446636036 1108 8 0.1314496850259701 1108 9 0.20495320932372607 1108 10-11 0.14001327028851307 1108 12-13 0.12567889401849897 1108 14-15 0.20777131503533042 1108 16-17 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2316 46-47 -1.239000118518554 2316 48-49 -1.0211350308543174 2316 50-51 -1.2015241424514969 2316 52-53 -1.1994792143349358 2316 54-55 -1.7510234223419943 2316 56-57 -1.2916101330207468 2316 58-59 -0.9466306975516758 2316 60-61 -0.8729239421122301 2316 62-63 -1.421679262883508 2316 64-65 -1.7114402268951707 2316 66-67 -1.2886630464930775 2316 68-69 -1.2840875217384138 2316 70-71 -1.164727330821968 2316 72-73 -1.224478203660908 2316 74-75 -1.101179257068864 2316 76-77 -1.0070284798638518 2316 78-79 -1.0261702066815843 2316 80-81 -0.9529461009709692 2316 82-83 -1.0153475398012546 2316 84-85 -1.1732634571434026 2316 86-87 -1.3802353183301186 2316 88-89 -0.997604935576657 2316 90-91 -0.9103549724240629 2316 92-93 -0.9309866099107325 2316 94-95 -1.1767940743062901 2316 96-97 -1.1545111638151653 2316 98-99 -1.1709099169445096 2316 100-101 -1.5124246739312888 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 32.0 4 76.0 5 150.0 6 350.0 7 1170.0 8 2853.0 9 6033.0 10 10005.0 11 15512.0 12 22670.0 13 32068.0 14 41182.0 15 54004.0 16 68017.0 17 92240.0 18 76633.0 19 42411.0 20 32102.0 21 30915.0 22 31448.0 23 33970.0 24 40578.0 25 50153.0 26 63404.0 27 80365.0 28 102484.0 29 130026.0 30 165039.0 31 208821.0 32 267617.0 33 354225.0 34 490058.0 35 724738.0 36 1132484.0 37 1798729.0 38 2084182.0 39 550440.0 40 268.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.351670285148934 22.69433129100511 16.802943271247596 22.15105515259836 2 14.041458651280648 22.104928809033815 40.22116742389378 23.632445115791757 3 16.351629789245315 27.507688603169854 32.18709147488676 23.953590132698068 4 11.60025242612762 19.056543203646033 39.61685950349632 29.726344866730024 5 11.942092471627525 38.54899854728761 35.77386299059596 13.735045990488908 6 28.188082971324857 40.10001957618133 18.05421810129146 13.65767935120235 7 25.03689692634431 34.417977931886966 23.015263327448157 17.52986181432057 8 25.009810515605395 35.906622901432264 20.59969520640329 18.483871376559048 9 24.76675953663536 16.94022232215054 22.504714597407443 35.78830354380666 10-11 23.011977484698225 28.03236994716749 29.011311116496017 19.944341451638262 12-13 24.303852341233455 25.616871459742324 29.432080970804158 20.647195228220063 14-15 21.40017430438361 27.358687733764654 27.85665580451476 23.38448215733698 16-17 19.613283549781347 30.91035124697071 28.467980098799917 21.00838510444803 18-19 19.590098031056343 28.756617643689143 32.07794167426831 19.575342650986208 20-21 21.077131195678373 27.66604695267052 31.852024518495803 19.4047973331553 22-23 20.443924083591014 27.674318528508824 32.03010209837513 19.85165528952503 24-25 20.25112961821244 27.832164445412804 31.898235547752595 20.01847038862216 26-27 20.003987886737455 27.911614216544628 31.94761247631414 20.136785420403776 28-29 19.93633516949167 27.936337885423224 32.12744463545239 19.999882309632717 30-31 20.224901168036997 28.247958083807607 31.75344957823039 19.773691169925005 32-33 19.90791184446373 28.021026929422938 32.12362741804681 19.94743380806652 34-35 20.173368131440654 27.914232567350606 31.896720349112716 20.01567895209602 36-37 20.53835248257179 28.014587018463377 31.464703293315953 19.982357205648885 38-39 20.240014475145284 28.24481394991206 31.569220264705507 19.94595131023715 40-41 20.073961784449974 28.141292332872386 31.634029595011082 20.150716287666555 42-43 20.136213094691104 28.120497261078704 31.412985110490677 20.33030453373952 44-45 20.375514031054117 28.349676507611786 30.986193361957582 20.288616099376515 46-47 20.598739787007368 28.627553707851693 30.44705481649287 20.326651688648067 48-49 20.614720557004755 28.55452208502232 30.232472876938488 20.598284481034437 50-51 20.63790257178152 28.73953431899225 29.869608910467033 20.752954198759195 52-53 20.634490726465092 28.849358595354097 29.75391652036353 20.762234157817282 54-55 20.769782490386433 29.148063631306094 29.601863312535126 20.480290565772354 56-57 20.715672428555926 28.82784341206619 29.594440107465548 20.86204405191234 58-59 20.63833928249137 28.60221094814812 29.862080242062554 20.89736952729795 60-61 20.762973431707344 28.877358056066576 29.661457705637744 20.69821080658834 62-63 20.546022372239538 28.85174515644205 29.76731775509941 20.834914716219004 64-65 20.579857756114187 28.75147512995118 29.82332274230859 20.845344371626044 66-67 20.536585374133747 28.731411792536875 29.68705221771281 21.04495061561657 68-69 20.84545616522988 28.984309767666556 29.385653986761163 20.784580080342398 70-71 20.91152302316022 28.723982622407384 29.344917263501596 21.019577090930795 72-73 20.692249356372365 28.88015920505198 29.54577292346353 20.881818515112123 74-75 20.79055968353487 29.016175973187643 29.30634972777893 20.886914615498558 76-77 20.76936773287269 29.13388575509664 29.1992000470281 20.897546465002566 78-79 20.843912389993132 29.072416053328915 29.395034022246218 20.688637534431738 80-81 20.72787451024767 29.080581054894694 29.32636105178291 20.865183383074726 82-83 20.586728669532278 29.045930829976683 29.516185737477624 20.85115476301341 84-85 20.539161083196905 29.06210720895612 29.29659370248883 21.10213800535814 86-87 20.647812381517593 29.132557547548416 29.160344068533973 21.059286002400018 88-89 20.453810059663965 29.139386054033707 29.24589551029119 21.16090837601114 90-91 20.31311528611738 29.025894860813512 29.345730485783523 21.315259367285584 92-93 20.401296958493266 29.142850159585144 29.278647319522367 21.177205562399227 94-95 20.296175941021673 28.940365600080387 29.4372755565654 21.326182902332537 96-97 20.265753851856864 29.087547663765445 29.306214275514158 21.34048420886353 98-99 20.121966743872555 29.031376730442148 29.30558606034868 21.541070465336617 100-101 20.075663172995533 29.16193703471129 29.140545133093443 21.621854659199737 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3132.0 1 2068.0 2 1569.0 3 2035.5 4 1561.0 5 1359.0 6 2494.0 7 5507.5 8 9436.0 9 11577.5 10 10422.5 11 8921.0 12 9465.5 13 10719.0 14 11817.5 15 12726.0 16 13727.5 17 14916.5 18 16511.5 19 18638.5 20 21726.0 21 26129.5 22 33160.5 23 44855.0 24 58111.0 25 73404.5 26 96048.5 27 123301.5 28 155778.5 29 184849.5 30 204249.0 31 226449.5 32 251130.0 33 272703.0 34 292023.0 35 307258.0 36 316234.0 37 322084.5 38 326232.5 39 326797.0 40 325845.0 41 318978.5 42 304203.5 43 292111.5 44 282863.0 45 272085.0 46 260166.0 47 248119.5 48 241211.5 49 236819.0 50 229327.5 51 217575.0 52 199646.5 53 184577.5 54 177368.5 55 169541.0 56 152724.0 57 134463.5 58 122126.0 59 110505.0 60 97960.0 61 83354.5 62 69033.5 63 56433.5 64 45720.0 65 37778.5 66 30899.0 67 24322.0 68 18556.0 69 14369.5 70 11502.0 71 8729.5 72 6477.0 73 5082.5 74 4152.0 75 3251.5 76 2338.0 77 1888.5 78 1647.5 79 1446.0 80 1053.5 81 535.0 82 331.0 83 255.0 84 169.0 85 106.5 86 78.0 87 50.0 88 30.5 89 27.0 90 21.0 91 12.0 92 9.0 93 7.0 94 4.0 95 2.0 96 1.5 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05164381563610437 2 0.0019236302931940716 3 2.942022801355639E-4 4 2.2630944625812608E-4 5 0.002206517101016729 6 0.0021159933225134786 7 0.0 8 1.1315472312906302E-5 9 1.2447019544196934E-4 10-11 5.657736156453151E-6 12-13 0.0 14-15 4.8656530945497103E-4 16-17 0.0 18-19 2.828868078226576E-5 20-21 5.318271987065962E-4 22-23 0.0 24-25 7.751098534340818E-4 26-27 0.00788122646593924 28-29 0.008033985342163475 30-31 0.0012277287459503338 32-33 0.006772310179274423 34-35 0.0087072559447814 36-37 0.004769471579890007 38-39 0.002715713355097513 40-41 0.002155597475608651 42-43 0.0014427227198955538 44-45 8.769491042502386E-4 46-47 7.015592834001908E-4 48-49 0.0021442820032957443 50-51 0.0013578566775487564 52-53 0.0023988801303361364 54-55 8.769491042502386E-4 56-57 2.2630944625812608E-4 58-59 8.147140065292538E-4 60-61 0.006449819218356593 62-63 0.0028854454397911075 64-65 0.0011315472312906304 66-67 0.005697340309548324 68-69 0.0073946611564842685 70-71 0.002427168811118402 72-73 5.544581433324088E-4 74-75 0.0038246296417623303 76-77 0.0021216510586699318 78-79 0.002540323534247465 80-81 9.278687296583168E-4 82-83 1.8104755700650084E-4 84-85 6.902438110872845E-4 86-87 0.004961834609209414 88-89 0.0024158533388054957 90-91 0.001188124592855162 92-93 1.301279315984225E-4 94-95 4.639343648291584E-4 96-97 3.507796417000954E-4 98-99 5.431426710195026E-4 100-101 5.657736156453151E-6 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8837457.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.12603686387433 #Duplication Level Percentage of deduplicated Percentage of total 1 67.96423717725328 34.7474209534935 2 18.0861772594539 18.493491305868115 3 6.459350000139619 9.907228986714145 4 2.8066432979657567 5.739701948621725 5 1.427265706514652 3.648521956290587 6 0.8705048187182299 2.670327687118108 7 0.5114629593287379 1.830435187920312 8 0.36965918503386874 1.5119367296889055 9 0.24723388058986392 1.137607964373248 >10 1.1397674580177475 10.104580847371873 >50 0.0700145945969391 2.485146147806179 >100 0.04262636310766843 4.154830120477169 >500 0.003453809549749539 1.1862020789025545 >1k 0.0015144070038303787 1.2800845491513608 >5k 2.2270681531558556E-5 0.08819686111747163 >10k+ 6.681204459467566E-5 1.0142866750846231 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37572 0.4251449257405156 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31689 0.35857600212368784 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19820 0.22427266124180295 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1315472312906304E-5 0.0 10-11 0.0 0.0 0.0 1.6973208469359456E-5 0.0 12-13 0.0 0.0 0.0 2.2630944625812608E-5 0.0 14-15 0.0 0.0 0.0 3.394641693871891E-5 0.0 16-17 0.0 1.1315472312906304E-5 0.0 3.394641693871891E-5 0.0 18-19 0.0 1.1315472312906304E-5 0.0 3.394641693871891E-5 0.0 20-21 0.0 1.1315472312906304E-5 0.0 6.789283387743782E-5 0.0 22-23 0.0 1.6973208469359456E-5 0.0 1.4710114006778193E-4 0.0 24-25 0.0 2.2630944625812608E-5 0.0 3.168332247613765E-4 0.0 26-27 0.0 2.2630944625812608E-5 0.0 4.582766286727053E-4 0.0 28-29 0.0 2.2630944625812608E-5 0.0 9.052377850325043E-4 0.0 30-31 0.0 2.2630944625812608E-5 0.0 0.002155597475608651 0.0 32-33 0.0 2.2630944625812608E-5 0.0 0.004752498371420647 0.0 34-35 0.0 2.2630944625812608E-5 0.0 0.008809095195597557 0.0 36-37 0.0 2.2630944625812608E-5 0.0 0.01595481596119789 1.1315472312906304E-5 38-39 0.0 3.394641693871891E-5 0.0 0.02631413086366361 1.1315472312906304E-5 40-41 0.0 3.394641693871891E-5 0.0 0.03903837947952675 1.1315472312906304E-5 42-43 0.0 3.394641693871891E-5 0.0 0.052051172639368995 1.1315472312906304E-5 44-45 0.0 3.394641693871891E-5 0.0 0.0672308787471328 1.1315472312906304E-5 46-47 0.0 3.394641693871891E-5 0.0 0.08685190773771233 1.1315472312906304E-5 48-49 0.0 3.394641693871891E-5 0.0 0.10943759047427332 1.1315472312906304E-5 50-51 0.0 3.394641693871891E-5 0.0 0.1329737728851184 1.1315472312906304E-5 52-53 0.0 3.394641693871891E-5 0.0 0.15785649650119937 1.1315472312906304E-5 54-55 0.0 3.394641693871891E-5 0.0 0.18499665684370514 1.1315472312906304E-5 56-57 0.0 4.5261889251625216E-5 0.0 0.21898267793551923 1.1315472312906304E-5 58-59 0.0 5.657736156453152E-5 0.0 0.2582021049720525 1.1315472312906304E-5 60-61 0.0 5.657736156453152E-5 0.0 0.29975251930504443 1.1315472312906304E-5 62-63 0.0 5.657736156453152E-5 0.0 0.34434113795405175 1.1315472312906304E-5 64-65 0.0 5.657736156453152E-5 0.0 0.39214335073992435 1.1315472312906304E-5 66-67 0.0 8.486604234679728E-5 0.0 0.44924688176700606 1.1315472312906304E-5 68-69 5.657736156453152E-6 1.0183925081615673E-4 0.0 0.5142938743577479 1.1315472312906304E-5 70-71 1.1315472312906304E-5 1.0749698697260988E-4 0.0 0.5870523613297355 1.1315472312906304E-5 72-73 1.1315472312906304E-5 1.1315472312906304E-4 0.0 0.6668490720803507 1.1315472312906304E-5 74-75 1.1315472312906304E-5 1.1315472312906304E-4 0.0 0.749667013938512 1.1315472312906304E-5 76-77 1.1315472312906304E-5 1.1315472312906304E-4 0.0 0.843557145454852 1.1315472312906304E-5 78-79 1.1315472312906304E-5 1.1315472312906304E-4 0.0 0.9539678665480352 1.1315472312906304E-5 80-81 1.1315472312906304E-5 1.2447019544196934E-4 0.0 1.0766275864199395 1.1315472312906304E-5 82-83 1.1315472312906304E-5 1.3578566775487565E-4 0.0 1.2089733505916918 1.1315472312906304E-5 84-85 1.1315472312906304E-5 1.3578566775487565E-4 0.0 1.3441253518970446 1.1315472312906304E-5 86-87 1.1315472312906304E-5 1.3578566775487565E-4 0.0 1.4934443245381561 1.1315472312906304E-5 88-89 1.1315472312906304E-5 1.3578566775487565E-4 0.0 1.6657280482383112 2.2630944625812608E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 21365 0.0 53.475918 1 GTATCAA 39260 0.0 40.9281 1 ATCAACG 50450 0.0 31.567736 3 TCAACGC 51015 0.0 31.1995 4 TATCAAC 52345 0.0 30.516869 2 CAACGCA 52950 0.0 30.07729 5 AACGCAG 55635 0.0 28.684679 6 CGCAGAG 61880 0.0 25.61296 8 ACGCAGA 62650 0.0 25.396576 7 GTGGTAT 10150 0.0 23.645693 1 GCAGAGT 66415 0.0 23.528057 9 TGGTATC 10030 0.0 22.968311 2 GTACATG 36565 0.0 22.108812 1 AGAGTAC 60455 0.0 21.994017 10-11 GAGTACT 41535 0.0 21.989807 12-13 TACATGG 35985 0.0 21.634443 2 AGTACTT 43490 0.0 20.973997 12-13 ACATGGG 36525 0.0 20.44247 3 CAGAGTA 65660 0.0 20.239658 10-11 GTACTTT 44915 0.0 20.134254 14-15 >>END_MODULE