FastQCFastQC Report
Wed 25 May 2016
SRR2049396_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049396_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4980690
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260070.5221565686681966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208830.4192792564885588No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117820.23655356988690324No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83380.1674065239956713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118350.055.629691
GTATCAA223550.043.8152851
GTCGTAT4450.043.7680639
GTACCTG46900.041.1211131
TACCTGG46100.040.491922
TATAACG6050.038.469582
ATCAACG278500.035.0327153
TCAACGC282450.034.526674
TATCAAC283500.034.3628652
CAACGCA290150.033.625765
ATAACGC7350.033.6058273
AACGCAG305200.032.2334826
TCTGTCG14000.031.217158
GTGGTAT49400.030.9628261
GTCTCGC14150.030.2132681
CGCAGAG335400.029.105988
TGGTATC50350.029.0554752
ACCTGGG62500.029.0323313
ACGCAGA340200.028.807027
TGTCGTA6900.028.2272268
Produced by FastQC (version 0.11.5)