##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049396_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4980690
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.441973100112634	31.0	31.0	34.0	30.0	34.0
2	31.452076318742986	31.0	31.0	34.0	29.0	34.0
3	31.468358801692133	33.0	31.0	34.0	28.0	34.0
4	35.10377859292588	37.0	35.0	37.0	32.0	37.0
5	35.14199719315998	37.0	35.0	37.0	33.0	37.0
6	35.23995169344006	37.0	35.0	37.0	33.0	37.0
7	35.19667134473336	37.0	35.0	37.0	32.0	37.0
8	35.16794640903168	37.0	35.0	37.0	32.0	37.0
9	36.74347851402115	39.0	37.0	39.0	33.0	39.0
10-11	36.66941728957233	39.0	37.0	39.0	32.5	39.0
12-13	36.63436622235072	39.0	37.0	39.0	32.0	39.0
14-15	37.77516247748806	40.0	38.0	41.0	32.5	41.0
16-17	37.76397296358537	40.0	38.0	41.0	32.5	41.0
18-19	37.81379517697347	40.0	38.0	41.0	33.0	41.0
20-21	37.77369782098464	40.0	38.0	41.0	33.0	41.0
22-23	37.63430870020017	40.0	38.0	41.0	32.0	41.0
24-25	37.518549036378495	40.0	37.0	41.0	32.0	41.0
26-27	37.36253631524949	40.0	37.0	41.0	32.0	41.0
28-29	37.19061947240242	40.0	37.0	41.0	32.0	41.0
30-31	36.96228735376022	40.0	36.5	41.0	31.0	41.0
32-33	36.643027672872634	39.5	36.0	41.0	30.5	41.0
34-35	36.39972162491542	39.0	35.5	41.0	30.0	41.0
36-37	36.17549445960299	39.0	35.0	41.0	30.0	41.0
38-39	35.940711327145436	39.0	35.0	41.0	29.5	41.0
40-41	35.703090435260975	39.0	35.0	40.0	28.5	41.0
42-43	35.44803862517041	39.0	35.0	40.0	27.0	41.0
44-45	35.129198564857475	38.5	35.0	40.0	25.5	41.0
46-47	34.71887880996408	38.0	34.5	40.0	24.0	41.0
48-49	34.37197697507775	38.0	34.0	40.0	22.5	41.0
50-51	33.002794090778586	36.5	32.5	39.0	19.5	40.0
52-53	33.27002995568887	37.5	33.0	39.0	21.0	40.0
54-55	34.298724072367484	38.5	34.0	40.0	22.0	41.0
56-57	34.41400077097751	39.0	34.0	40.0	22.0	41.0
58-59	34.25845655923175	38.0	34.0	40.0	22.0	41.0
60-61	33.99627069743349	38.0	34.0	40.0	20.0	41.0
62-63	33.71805512890784	38.0	34.0	40.0	19.5	41.0
64-65	33.43320624250857	37.0	33.5	40.0	18.0	41.0
66-67	33.11399153530937	37.0	33.0	40.0	16.0	41.0
68-69	32.76542155805721	36.0	33.0	39.0	13.0	41.0
70-71	32.42307361028291	36.0	32.5	39.0	9.5	41.0
72-73	32.15957307120098	35.0	32.0	39.0	8.0	40.0
74-75	31.971660051117414	35.0	32.0	38.5	7.0	40.0
76-77	31.646508013949877	35.0	32.0	37.5	3.5	40.0
78-79	31.281077019449114	35.0	32.0	37.0	2.0	39.0
80-81	30.950308089842974	35.0	32.0	36.5	2.0	39.0
82-83	30.619418695000093	35.0	31.0	36.0	2.0	38.5
84-85	30.3050913628433	35.0	31.0	36.0	2.0	37.0
86-87	30.0094799114179	35.0	31.0	35.0	2.0	37.0
88-89	29.716938315775526	35.0	31.0	35.0	2.0	36.5
90-91	29.45436917374902	34.0	31.0	35.0	2.0	36.0
92-93	29.199868190953463	34.0	30.0	35.0	2.0	36.0
94-95	28.93973465523853	34.0	30.0	35.0	2.0	36.0
96-97	28.682716952871992	34.0	30.0	35.0	2.0	35.0
98-99	28.3669724475926	34.0	29.5	35.0	2.0	35.0
100-101	27.834403968124896	34.0	28.5	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.19931355540482087
1101	2	0.20758505758059798
1101	3	0.2158837614389988
1101	4	0.1797494908635855
1101	5	0.24175169839323019
1101	6	0.24531479228608788
1101	7	0.19295579111141592
1101	8	0.17889902604662922
1101	9	0.248776435385885
1101	10-11	0.14911639117679698
1101	12-13	0.1894195089313726
1101	14-15	0.27636679597861047
1101	16-17	0.21177126480840514
1101	18-19	0.2641961395851311
1101	20-21	0.13513878866366014
1101	22-23	0.16302571137660493
1101	24-25	0.23133064285956806
1101	26-27	0.2238304291671369
1101	28-29	0.20946502778787846
1101	30-31	0.17653275030775717
1101	32-33	0.07436174734370837
1101	34-35	0.11446835032762692
1101	36-37	0.11878570296859436
1101	38-39	0.04947678847779713
1101	40-41	-0.029594779661145765
1101	42-43	-0.0027484644059185825
1101	44-45	0.0011065531287357544
1101	46-47	-0.10814929339094448
1101	48-49	-0.16233917605784143
1101	50-51	-0.20588293132947655
1101	52-53	-0.09586057503162948
1101	54-55	0.10905488746519154
1101	56-57	0.034618077298802064
1101	58-59	0.07894392204864431
1101	60-61	0.08259225622803257
1101	62-63	0.051001239413409394
1101	64-65	-0.04112433118803693
1101	66-67	0.0052420733002236375
1101	68-69	0.0774419129706061
1101	70-71	0.027696256924159002
1101	72-73	-0.13607488661887146
1101	74-75	-0.16058134017122327
1101	76-77	-0.14417690309122477
1101	78-79	-0.1752384999420009
1101	80-81	-0.28801190673959454
1101	82-83	-0.3594054538164855
1101	84-85	-0.31325068119380006
1101	86-87	-0.43995627053565656
1101	88-89	-0.5425982194458498
1101	90-91	-0.5768935312475811
1101	92-93	-0.6208387239658677
1101	94-95	-0.6745843607904511
1101	96-97	-0.7095254041001056
1101	98-99	-0.7725919863186306
1101	100-101	-0.9282579393804333
1102	1	0.3133677362472085
1102	2	0.2956100541913358
1102	3	0.2593486411578745
1102	4	0.242080011647829
1102	5	0.2017560882938767
1102	6	0.3041654130051299
1102	7	0.2884072084683069
1102	8	0.2763667850238676
1102	9	0.3249805915888757
1102	10-11	0.36115197089115014
1102	12-13	0.2989419350831284
1102	14-15	0.43938747800562084
1102	16-17	0.3922617716482577
1102	18-19	0.3341970003499668
1102	20-21	0.24726248917775706
1102	22-23	0.26871616312750746
1102	24-25	0.3058245669358044
1102	26-27	0.2749489069906019
1102	28-29	0.3208793504843115
1102	30-31	0.3085640046788285
1102	32-33	0.35999248353434865
1102	34-35	0.4186231976171868
1102	36-37	0.4351478310374901
1102	38-39	0.27799330332705807
1102	40-41	0.32789159335559503
1102	42-43	0.17591134626885463
1102	44-45	0.37471890338255776
1102	46-47	0.2565412078539353
1102	48-49	0.2043673009823337
1102	50-51	0.1638935495626228
1102	52-53	0.2806097942149606
1102	54-55	0.4503468739597949
1102	56-57	0.43529752201940397
1102	58-59	0.33540303679505
1102	60-61	0.43973598028404837
1102	62-63	0.41380242640811105
1102	64-65	0.3491951416795871
1102	66-67	0.34599866406894364
1102	68-69	0.31592890358611925
1102	70-71	0.25464344684976936
1102	72-73	0.25263448259114085
1102	74-75	0.27552894962677854
1102	76-77	0.2763315291762716
1102	78-79	0.29146014413819543
1102	80-81	0.16628927057150023
1102	82-83	0.17021720840002885
1102	84-85	0.07764229776265097
1102	86-87	0.12576417886966595
1102	88-89	0.16223712700123372
1102	90-91	0.012065847108058136
1102	92-93	-0.06214284724460484
1102	94-95	-0.12731855876205955
1102	96-97	-0.20026477957218347
1102	98-99	-0.29917888160443695
1102	100-101	-0.28637572644012366
1103	1	0.21960451084183674
1103	2	0.21527071568434053
1103	3	0.26743101991312557
1103	4	0.2553056436671639
1103	5	0.2551907026314737
1103	6	0.2677772155289304
1103	7	0.24065765889960034
1103	8	0.2743008298989551
1103	9	0.3497501712877096
1103	10-11	0.27585846507154343
1103	12-13	0.2944187897841033
1103	14-15	0.3028275310626043
1103	16-17	0.3416332200281005
1103	18-19	0.32414944225584463
1103	20-21	0.29051510215727916
1103	22-23	0.306922754683967
1103	24-25	0.34483748840668227
1103	26-27	0.3091057995804647
1103	28-29	0.33966425782787013
1103	30-31	0.3161559591323737
1103	32-33	0.28202779424891133
1103	34-35	0.3597163406522057
1103	36-37	0.2610052329087438
1103	38-39	0.2223423016392374
1103	40-41	0.24955757142549828
1103	42-43	0.17243896007049386
1103	44-45	0.25908611674807247
1103	46-47	0.15545102030423408
1103	48-49	0.1590623317367701
1103	50-51	0.0209165483648448
1103	52-53	0.19656857846378983
1103	54-55	0.35355891206528156
1103	56-57	0.42469317307136834
1103	58-59	0.42760906923236774
1103	60-61	0.35648042830265325
1103	62-63	0.3534564186910458
1103	64-65	0.3271649696853345
1103	66-67	0.3305544194315786
1103	68-69	0.32035430193584347
1103	70-71	0.2477517100026887
1103	72-73	0.3758737085243169
1103	74-75	0.33354489589595104
1103	76-77	0.3475270979922236
1103	78-79	0.2718119359032336
1103	80-81	0.359744700229097
1103	82-83	0.34300653463096253
1103	84-85	0.2697388717485616
1103	86-87	0.1691046281149724
1103	88-89	0.16145210196573245
1103	90-91	0.23769314804528108
1103	92-93	0.18829182173567816
1103	94-95	0.12461118112638658
1103	96-97	0.13312744094097795
1103	98-99	0.03240137337993687
1103	100-101	0.12058345662614656
1104	1	-0.1185925376340613
1104	2	-0.05799489646963707
1104	3	0.015492040743453117
1104	4	-0.07798206819396114
1104	5	-0.1161824402782301
1104	6	-0.176005355704703
1104	7	-0.16207733570534089
1104	8	-0.14381484798590805
1104	9	-0.1544736444314907
1104	10-11	-0.17307114729726436
1104	12-13	-0.26144752680802696
1104	14-15	-0.14106375247627057
1104	16-17	-0.23328404393352997
1104	18-19	-0.3033339875592347
1104	20-21	-0.32936739744331334
1104	22-23	-0.27273870948317125
1104	24-25	-0.22295821046218833
1104	26-27	-0.25276496876158205
1104	28-29	-0.2881621322370833
1104	30-31	-0.2761337549852314
1104	32-33	-0.3294315496666371
1104	34-35	-0.2773140915204664
1104	36-37	-0.28977512204043165
1104	38-39	-0.4931245482805551
1104	40-41	-0.49720923150884033
1104	42-43	-0.4488159199951198
1104	44-45	-0.39836646442691404
1104	46-47	-0.4366974936918524
1104	48-49	-0.4403106254369362
1104	50-51	-0.5129549172064998
1104	52-53	-0.490404238784528
1104	54-55	-0.45734757153580574
1104	56-57	-0.45876048239937717
1104	58-59	-0.3424675620536206
1104	60-61	-0.38324542967340136
1104	62-63	-0.37265182943403374
1104	64-65	-0.42846709097009494
1104	66-67	-0.3670625932509779
1104	68-69	-0.358691128498954
1104	70-71	-0.3463155831740039
1104	72-73	-0.4430275950882745
1104	74-75	-0.44538488510579555
1104	76-77	-0.38723343882957906
1104	78-79	-0.4330429066954906
1104	80-81	-0.3958665410508395
1104	82-83	-0.39103343601251694
1104	84-85	-0.3668462815351887
1104	86-87	-0.3828435099815053
1104	88-89	-0.4135537742130886
1104	90-91	-0.44057669072164174
1104	92-93	-0.44378877394301597
1104	94-95	-0.4927611651325172
1104	96-97	-0.5252884059844227
1104	98-99	-0.5409979343298765
1104	100-101	-0.5087836071857588
1105	1	0.295156264550414
1105	2	0.3415514490584073
1105	3	0.3155610437118028
1105	4	0.25809575848578703
1105	5	0.3079818998545818
1105	6	0.36575769369256506
1105	7	0.3734441172452847
1105	8	0.33586633757114726
1105	9	0.42280512599026565
1105	10-11	0.3505530255386802
1105	12-13	0.2812733824105109
1105	14-15	0.4229104321847146
1105	16-17	0.33714213027117523
1105	18-19	0.37309988124592053
1105	20-21	0.49394703937845463
1105	22-23	0.38229980277890974
1105	24-25	0.40530105696310414
1105	26-27	0.4214713345260961
1105	28-29	0.4112533840398598
1105	30-31	0.43629900481000305
1105	32-33	0.4251270983023332
1105	34-35	0.5130370588378454
1105	36-37	0.36914150937096935
1105	38-39	0.3336699529021061
1105	40-41	0.337831626916973
1105	42-43	0.3936178668356476
1105	44-45	0.2676232067992288
1105	46-47	0.3165811287990934
1105	48-49	0.23535328079692164
1105	50-51	0.18379839419790045
1105	52-53	0.26173333780734964
1105	54-55	0.4139095984756338
1105	56-57	0.44280389372678997
1105	58-59	0.3192829571960729
1105	60-61	0.3280541361763838
1105	62-63	0.36726017686261514
1105	64-65	0.40485320812023673
1105	66-67	0.4282618274868426
1105	68-69	0.36568433496261576
1105	70-71	0.30984567993522205
1105	72-73	0.39232074584104026
1105	74-75	0.4218631166505311
1105	76-77	0.31403282217375406
1105	78-79	0.3780831780911633
1105	80-81	0.363554586232155
1105	82-83	0.41022215418585617
1105	84-85	0.3730129596331686
1105	86-87	0.32498910426991756
1105	88-89	0.25541563488971164
1105	90-91	0.28343232206479385
1105	92-93	0.2818315775041462
1105	94-95	0.3153047385127081
1105	96-97	0.30098672772649593
1105	98-99	0.30795313103889654
1105	100-101	0.24674585632793722
1106	1	0.31223566925024215
1106	2	0.37340017288374483
1106	3	0.38188343750057996
1106	4	0.3833330911656532
1106	5	0.3332629552532609
1106	6	0.3147069870530075
1106	7	0.3798787337343512
1106	8	0.448887982691474
1106	9	0.40819284139431034
1106	10-11	0.3983234993112248
1106	12-13	0.4235905946930174
1106	14-15	0.49514481191890525
1106	16-17	0.4615895016999474
1106	18-19	0.4970839454071907
1106	20-21	0.5163580592132462
1106	22-23	0.5090389760237741
1106	24-25	0.5359577034970542
1106	26-27	0.5752232171199836
1106	28-29	0.5477630889954099
1106	30-31	0.6067635879830533
1106	32-33	0.6562759499398467
1106	34-35	0.7157834771999987
1106	36-37	0.6932382996295061
1106	38-39	0.672655381617453
1106	40-41	0.6208492052862269
1106	42-43	0.7324618536637431
1106	44-45	0.7151241257361676
1106	46-47	0.5887777300146553
1106	48-49	0.5415412485959763
1106	50-51	0.5882982091441065
1106	52-53	0.704387279928099
1106	54-55	0.7915127386596623
1106	56-57	0.828518611376488
1106	58-59	0.8808951461782684
1106	60-61	0.8384948349040542
1106	62-63	0.790058115847728
1106	64-65	0.8265710993871309
1106	66-67	0.8289977606281269
1106	68-69	0.903219228446666
1106	70-71	0.9306063808411196
1106	72-73	0.9075143510530594
1106	74-75	0.8733103592223337
1106	76-77	0.9016961214628871
1106	78-79	0.8724978857045862
1106	80-81	0.8285974256317381
1106	82-83	0.9080586552792411
1106	84-85	0.8784848293983529
1106	86-87	0.8457404179302443
1106	88-89	0.8403580972481244
1106	90-91	0.7868904060345763
1106	92-93	0.846027950133152
1106	94-95	0.8069863408727862
1106	96-97	0.851594350043257
1106	98-99	0.8099669168342984
1106	100-101	0.8089637838457371
1107	1	0.2928766292920564
1107	2	0.2363856829883879
1107	3	0.31158575383225795
1107	4	0.29136154707072137
1107	5	0.23901589737793927
1107	6	0.3215229194046003
1107	7	0.36139863711469644
1107	8	0.36965981950292814
1107	9	0.43456213375791464
1107	10-11	0.3752807357717174
1107	12-13	0.3879518694081483
1107	14-15	0.5769863355957199
1107	16-17	0.4340231107957706
1107	18-19	0.4723062304522685
1107	20-21	0.53330240918595
1107	22-23	0.5994280252262811
1107	24-25	0.47467514229463603
1107	26-27	0.5374829578515516
1107	28-29	0.5395497084798038
1107	30-31	0.5393891770491095
1107	32-33	0.6140307626049335
1107	34-35	0.6055346666280101
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2312	96-97	0.38520341581131845
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2313	1	-0.2844347290459517
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2314	4	-0.8165866667481652
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2314	7	-1.1001074822708503
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2314	98-99	-0.8457981959958865
2314	100-101	-0.869985065526695
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2315	3	-1.0549661130555066
2315	4	-0.9529462302746765
2315	5	-0.5762286192856294
2315	6	-0.6321229632288023
2315	7	-0.547994991008224
2315	8	-0.9475025794574918
2315	9	-0.8377084244445072
2315	10-11	-0.6315201541712483
2315	12-13	-0.7129759652217373
2315	14-15	-1.0630769540088139
2315	16-17	-1.4930053716443439
2315	18-19	-0.9352082980959011
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2315	24-25	-0.6378878011138553
2315	26-27	-0.7198998920343413
2315	28-29	-0.8285282329596058
2315	30-31	-1.2061779066888505
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2315	100-101	-0.1859719881454076
2316	1	-0.9479997107330611
2316	2	-1.090246008036857
2316	3	-1.2693424353820184
2316	4	-0.8935937702581498
2316	5	-0.7258220938129298
2316	6	-0.7172746753754495
2316	7	-0.8380011954390412
2316	8	-0.7712509880612757
2316	9	-0.8691486437751692
2316	10-11	-0.8957097680231314
2316	12-13	-0.830927526056243
2316	14-15	-1.2528296213960672
2316	16-17	-1.197534821970919
2316	18-19	-1.1142417033154501
2316	20-21	-1.1200576639807807
2316	22-23	-1.3224754133373224
2316	24-25	-1.2176110679596306
2316	26-27	-1.4164223963337008
2316	28-29	-1.3806764236025373
2316	30-31	-1.096813626310734
2316	32-33	-0.9949102649145019
2316	34-35	-1.20329903375238
2316	36-37	-1.074859820121354
2316	38-39	-1.3392758959439348
2316	40-41	-1.3394507026319644
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2316	44-45	-1.1613048413597724
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2316	48-49	-0.7997778458386975
2316	50-51	-1.1799431681856092
2316	52-53	-1.3806930240080248
2316	54-55	-1.2581217082961587
2316	56-57	-1.2540381638837559
2316	58-59	-1.1023630386373142
2316	60-61	-1.1622420150200483
2316	62-63	-1.314550477832725
2316	64-65	-1.2456083539801384
2316	66-67	-1.1621703400928887
2316	68-69	-1.148719481517901
2316	70-71	-1.3661028072511598
2316	72-73	-1.2862924031564766
2316	74-75	-1.1282093890472957
2316	76-77	-1.0582709597423197
2316	78-79	-1.1744545508245317
2316	80-81	-0.9716497096677728
2316	82-83	-0.9514798260144985
2316	84-85	-1.0648823785321078
2316	86-87	-0.9649000152451919
2316	88-89	-0.9039602793574382
2316	90-91	-0.9184676604760362
2316	92-93	-0.975570654084688
2316	94-95	-1.275417628809386
2316	96-97	-1.0229581720720162
2316	98-99	-0.9407667754837554
2316	100-101	-0.8793925288402598
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	45950.0
3	10120.0
4	8132.0
5	7407.0
6	7826.0
7	8825.0
8	9908.0
9	11774.0
10	15129.0
11	19018.0
12	25386.0
13	30842.0
14	30033.0
15	34102.0
16	42336.0
17	54478.0
18	44459.0
19	25886.0
20	23211.0
21	22688.0
22	23447.0
23	25399.0
24	28975.0
25	33850.0
26	40196.0
27	48996.0
28	59447.0
29	73015.0
30	90723.0
31	114142.0
32	147901.0
33	198640.0
34	280950.0
35	426784.0
36	656331.0
37	989152.0
38	1006965.0
39	258139.0
40	128.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.75206628165688	19.8921477280942	14.100856231776962	21.254929758471956
2	16.339000134525456	20.85214849200979	40.33109325023542	22.477758123229332
3	17.84961206752888	27.409383705189793	30.940249846099977	23.80075438118135
4	12.304709853204857	18.036045225192304	39.39089352081455	30.26835140078829
5	11.893421088296739	37.1298264142306	34.82748339284836	16.149269104624302
6	27.385061383186816	38.54970951864064	19.420446819643743	14.644782278528796
7	25.252579807589605	33.354006863424594	23.56180093819946	17.83161239078634
8	25.158950223773164	33.75414868818128	21.967637722392777	19.119263365652778
9	25.259072637524007	16.930576826038163	23.136814488349486	34.67353604808835
10-11	23.11058169425775	26.898438805920698	29.31714763451564	20.67383186530591
12-13	24.748688992192836	24.85396518054826	29.44087862547007	20.956467201788833
14-15	22.081811906462818	26.53487927570644	28.1202050708741	23.263103746956645
16-17	20.02549606696362	29.870385288179047	28.34219745657758	21.76192118827976
18-19	19.901941306336237	27.5391369899306	32.04215090232651	20.51677080140665
20-21	21.53033392261444	26.898443876244944	31.76893755728425	19.802284643856368
22-23	20.83427949820836	27.106163011809947	31.95374965390945	20.10580783607224
24-25	20.498300813701917	27.19255269485425	31.931982350549408	20.37716414089442
26-27	20.264054710298456	27.072976344680583	31.95912087104565	20.703848073975315
28-29	20.42155303083159	27.07354023452139	31.99167900523917	20.513227729407852
30-31	20.837832400592532	27.71452871124361	31.441824400049633	20.005814488114233
32-33	20.150121721671475	27.373241310076544	32.08802108169155	20.38861588656042
34-35	20.259071014185327	26.875478041968208	31.96645747849633	20.89899346535013
36-37	21.18054213547976	27.476818391129537	30.99938290796615	20.343256565424557
38-39	20.88264599041178	27.600631852535617	31.286197375111495	20.230524781941103
40-41	20.46886751955706	27.382325339711205	31.44020725405246	20.708599886679274
42-43	20.38096603406572	27.581929221055752	31.049518838184305	20.98758590669422
44-45	20.942025769938713	27.807487060515847	30.685577311723655	20.56490985782179
46-47	21.009384851606733	28.392858344092215	29.939296105506962	20.658460698794084
48-49	20.93905104216581	27.932605704155762	30.157648303461865	20.97069495021656
50-51	21.15394461684264	27.813838059029454	29.760069231456544	21.272148092671365
52-53	21.05437328225436	28.209954174707995	29.535546278002755	21.200126265034886
54-55	21.16837852473997	28.93851346345101	29.242252564674825	20.650855447134198
56-57	20.847750248448644	28.51322045965936	29.19938948746242	21.43963980442958
58-59	20.731812364391626	27.93670063092154	29.818973988491535	21.5125130161953
60-61	21.27767866581229	28.4405541199351	29.246277344871928	21.03548986938068
62-63	21.052485220828483	28.366994282887305	29.354731940006506	21.225788556277706
64-65	20.90988024784671	28.215658080682797	29.638715663250764	21.235746008219728
66-67	20.925004324312493	28.240486186243498	29.24802339013852	21.58648609930549
68-69	21.60578204009762	28.601984301072463	28.85193435724045	20.94029930158947
70-71	21.643856684619102	28.2586845516415	29.018216396202202	21.079242367537194
72-73	21.16166356247093	28.426324851431783	29.421832543288495	20.990179042808787
74-75	21.126121748795732	28.572369361540822	29.15127365771624	21.150235231947214
76-77	21.284754945027696	28.734666651808066	28.836689017817367	21.14388938534687
78-79	21.375351768578614	28.887113697086132	28.946045067539668	20.79148946679559
80-81	21.214502537028324	28.61099079746163	29.00168050892774	21.172826156582307
82-83	20.852704935866758	28.695775451963897	29.24488225730388	21.206637354865467
84-85	21.058302367859785	28.662496350605327	28.949930104303412	21.329271177231472
86-87	21.302373826348816	28.772379235555068	28.81318922704571	21.112057711050404
88-89	20.903445928099426	28.78268690453758	28.956644277150794	21.357222890212196
90-91	20.66035265871389	28.673819401248775	29.066666318647314	21.59916162139002
92-93	21.02250174023925	28.97199695540952	28.716808713423603	21.288692590927628
94-95	20.95295900202053	28.692451858687974	28.985146136824035	21.369443002467463
96-97	20.96408953507668	28.766198740692754	28.93704176538729	21.332669958843276
98-99	20.864711907766793	28.649064714096355	28.869590254791028	21.616633123345817
100-101	20.947538961477715	28.94574222610252	28.573343318320042	21.53337549409972
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3652.0
1	2447.5
2	3952.5
3	5507.5
4	3116.0
5	2029.0
6	2830.5
7	5112.5
8	8485.0
9	10062.5
10	8624.5
11	7168.5
12	7311.5
13	7840.0
14	8327.0
15	8752.0
16	9188.0
17	10022.0
18	10929.0
19	11849.5
20	13358.0
21	15634.0
22	19417.0
23	26266.5
24	33436.0
25	40361.0
26	50966.0
27	64285.0
28	81417.0
29	96364.5
30	103340.5
31	112104.5
32	123135.0
33	132084.0
34	141578.0
35	150092.5
36	154059.0
37	156423.0
38	160077.5
39	161649.0
40	164007.5
41	162880.5
42	156502.5
43	153649.0
44	150456.5
45	147448.0
46	147547.0
47	147234.0
48	147286.0
49	147568.5
50	145341.5
51	138403.5
52	128971.0
53	120161.0
54	113245.0
55	109121.0
56	102074.0
57	91607.0
58	83403.5
59	76530.0
60	68623.5
61	58590.5
62	48491.0
63	40080.5
64	33222.5
65	28108.0
66	22917.0
67	17644.0
68	13283.0
69	9875.0
70	7381.0
71	5587.0
72	4426.5
73	3590.0
74	2877.5
75	2292.0
76	1743.0
77	1402.5
78	1175.0
79	1013.5
80	801.0
81	517.5
82	381.5
83	306.5
84	258.0
85	216.0
86	170.5
87	143.5
88	120.0
89	114.0
90	102.5
91	79.0
92	63.0
93	54.5
94	47.0
95	37.0
96	29.5
97	23.5
98	17.0
99	11.5
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014656603803890625
2	0.004417058680624572
3	0.003694267260158733
4	0.005621711048067637
5	0.003112018615894585
6	0.006746053257681166
7	0.00172666839333506
8	0.004015507891476883
9	0.010500553136212051
10-11	0.007378495750588774
12-13	0.009165396762295986
14-15	0.003373026628840583
16-17	0.006304347389618707
18-19	0.004085779279577729
20-21	9.034892755822988E-4
22-23	0.0011444197490709119
24-25	0.0073283019019453124
26-27	0.0011143034398848352
28-29	7.127526507371469E-4
30-31	5.621711048067637E-4
32-33	0.0013050400647299871
34-35	0.0017367071630637523
36-37	0.001435544071202986
38-39	0.0019575600970949806
40-41	0.002730545366204281
42-43	0.002830933063491203
44-45	0.007850317927837308
46-47	0.013984006232068249
48-49	0.005390819344307716
50-51	0.005922874139928404
52-53	0.013833424686137864
54-55	0.008613264427217915
56-57	0.005712059975625867
58-59	0.0071476040468288525
60-61	0.001435544071202986
62-63	0.002339033346785285
64-65	0.0022386456494983627
66-67	0.0026000413597312825
68-69	0.0019073662484515197
70-71	0.0024494598138008992
72-73	0.0013150788344586794
74-75	0.001485737919846447
76-77	0.008221752407798918
78-79	0.006434851396091706
80-81	0.013070478186757257
82-83	0.015750829704318077
84-85	0.00827194625644238
86-87	0.0058626415215562505
88-89	0.0032023675434528147
90-91	0.0024896148927156677
92-93	0.0010641095912413742
94-95	0.0013351563739160638
96-97	0.0018973274787228277
98-99	0.006314386159347399
100-101	0.010972375313460585
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4980690.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.92762584239165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.1564566220942	42.033221856004225
2	15.429363443542268	17.25855331313415
3	4.524240885565122	7.5909015440621035
4	1.7365929323889993	3.884940790527748
5	0.8451454833885489	2.3633490188670994
6	0.5099470704548476	1.7112077373493468
7	0.3081479819583094	1.2063789527336666
8	0.22341087422631292	0.9995871826280679
9	0.1740995687457957	0.8763277986122945
>10	0.9565010276396712	10.037639348911895
>50	0.08261476409838003	3.204797547469461
>100	0.04862802816113509	5.042298005789605
>500	0.0035935703214120397	1.3391886058223204
>1k	0.0011140048530387948	1.1048446338509934
>5k	3.593564042039342E-5	0.16757671142229322
>10k+	1.0780692126118028E-4	1.1791869528146783
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26007	0.5221565686681966	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20883	0.4192792564885588	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11782	0.23655356988690324	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8338	0.1674065239956713	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.0077539457384417E-5	0.0
10-11	0.0	0.0	0.0	2.0077539457384417E-5	0.0
12-13	0.0	0.0	0.0	5.0193848643461045E-5	0.0
14-15	0.0	0.0	0.0	8.031015782953767E-5	0.0
16-17	0.0	0.0	0.0	8.031015782953767E-5	0.0
18-19	0.0	0.0	0.0	1.0038769728692209E-4	0.0
20-21	0.0	0.0	0.0	1.4054277620169093E-4	0.0
22-23	0.0	0.0	0.0	2.8108555240338186E-4	0.0
24-25	0.0	0.0	0.0	5.220160258919949E-4	0.0
26-27	0.0	2.0077539457384417E-5	0.0	7.830240388379923E-4	0.0
28-29	0.0	2.0077539457384417E-5	0.0	0.0012749237555439107	0.0
30-31	0.0	2.0077539457384417E-5	0.0	0.0022888394981418238	0.0
32-33	0.0	2.0077539457384417E-5	0.0	0.004768415621128799	0.0
34-35	0.0	2.0077539457384417E-5	0.0	0.008522915499659686	0.0
36-37	0.0	2.0077539457384417E-5	0.0	0.013913734843967403	0.0
38-39	0.0	2.0077539457384417E-5	0.0	0.02294862759979039	0.0
40-41	0.0	2.0077539457384417E-5	0.0	0.03603918332600503	0.0
42-43	0.0	4.0155078914768835E-5	0.0	0.05010349971590282	0.0
44-45	0.0	4.0155078914768835E-5	0.0	0.06489060752626644	0.0
46-47	0.0	4.0155078914768835E-5	0.0	0.08231791177527611	0.0
48-49	0.0	4.0155078914768835E-5	0.0	0.10356998729091753	0.0
50-51	0.0	4.0155078914768835E-5	0.0	0.12968082735524594	0.0
52-53	0.0	4.0155078914768835E-5	0.0	0.15480786798616256	0.0
54-55	0.0	6.0232618372153255E-5	0.0	0.1834886331010362	0.0
56-57	0.0	6.0232618372153255E-5	0.0	0.2176405277180471	0.0
58-59	0.0	6.0232618372153255E-5	0.0	0.255707542529248	0.0
60-61	0.0	6.0232618372153255E-5	0.0	0.29631436608180795	0.0
62-63	0.0	6.0232618372153255E-5	0.0	0.3411776279993334	0.0
64-65	0.0	6.0232618372153255E-5	0.0	0.3925962065496949	0.0
66-67	0.0	6.0232618372153255E-5	0.0	0.4529492901585925	0.0
68-69	0.0	6.0232618372153255E-5	0.0	0.5199380005581556	0.0
70-71	0.0	6.0232618372153255E-5	0.0	0.5959415261740844	0.0
72-73	0.0	6.0232618372153255E-5	0.0	0.6861097558771978	0.0
74-75	0.0	7.027138810084547E-5	0.0	0.7778641111974445	0.0
76-77	0.0	8.031015782953767E-5	0.0	0.8771375050444818	0.0
78-79	0.0	8.031015782953767E-5	0.0	0.989621919854478	0.0
80-81	0.0	8.031015782953767E-5	0.0	1.1142632848059204	0.0
82-83	0.0	1.0038769728692209E-4	0.0	1.2543141612909054	0.0
84-85	0.0	1.0038769728692209E-4	0.0	1.3957403492287215	0.0
86-87	0.0	1.0038769728692209E-4	0.0	1.5503374030505814	0.0
88-89	0.0	1.0038769728692209E-4	0.0	1.7266583545653313	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11835	0.0	55.62969	1
GTATCAA	22355	0.0	43.815285	1
GTCGTAT	445	0.0	43.768063	9
GTACCTG	4690	0.0	41.121113	1
TACCTGG	4610	0.0	40.49192	2
TATAACG	605	0.0	38.46958	2
ATCAACG	27850	0.0	35.032715	3
TCAACGC	28245	0.0	34.52667	4
TATCAAC	28350	0.0	34.362865	2
CAACGCA	29015	0.0	33.62576	5
ATAACGC	735	0.0	33.605827	3
AACGCAG	30520	0.0	32.233482	6
TCTGTCG	1400	0.0	31.21715	8
GTGGTAT	4940	0.0	30.962826	1
GTCTCGC	1415	0.0	30.213268	1
CGCAGAG	33540	0.0	29.10598	8
TGGTATC	5035	0.0	29.055475	2
ACCTGGG	6250	0.0	29.032331	3
ACGCAGA	34020	0.0	28.80702	7
TGTCGTA	690	0.0	28.227226	8
>>END_MODULE