FastQCFastQC Report
Wed 25 May 2016
SRR2049396_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049396_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4980690
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264720.5314926245158803No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215110.4318879512677962No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148740.2986333218891358No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85700.17206451314978446No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50490.10137149672033394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113000.055.7633321
TATAACG7100.047.49982
GTATCAA218550.043.813491
ATAACGC8800.040.4815673
GTACCTG44550.039.573991
TACCTGG45350.037.2876052
ATCAACG285700.033.0510183
TCAACGC288900.032.7671364
TATCAAC291250.032.5526542
CAACGCA295200.032.13225
AACGCAG315950.030.367386
TCTGTCG13200.029.5053778
GTATAAC15400.028.080471
CGCAGAG351350.027.131188
ACGCAGA354400.027.0585087
ACCTGGG61700.026.2511253
GTGGTAT49350.026.1918051
GTCTCGC14000.026.1364351
GTCGTAT4400.025.9074239
TGGTATC49700.025.1357082
Produced by FastQC (version 0.11.5)