##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049396_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4980690 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04526421038049 33.0 31.0 34.0 30.0 34.0 2 32.20979201676876 33.0 31.0 34.0 30.0 34.0 3 32.27296238071432 34.0 31.0 34.0 30.0 34.0 4 35.931114564447896 37.0 35.0 37.0 35.0 37.0 5 35.828543033194194 37.0 35.0 37.0 35.0 37.0 6 35.86445010631057 37.0 35.0 37.0 35.0 37.0 7 35.79401207463223 37.0 35.0 37.0 35.0 37.0 8 35.782114526300575 37.0 35.0 37.0 33.0 37.0 9 37.38245082508649 39.0 37.0 39.0 34.0 39.0 10-11 37.37163164139908 39.0 37.0 39.0 34.0 39.0 12-13 37.3586507090383 39.0 37.0 39.0 34.0 39.0 14-15 38.54855431677137 40.0 38.0 41.0 34.0 41.0 16-17 38.546112185259474 40.0 38.0 41.0 34.0 41.0 18-19 38.60779068763565 40.0 38.0 41.0 34.0 41.0 20-21 38.59248186496248 40.0 38.0 41.0 34.0 41.0 22-23 38.482141831754234 40.0 38.0 41.0 34.0 41.0 24-25 38.37768220868996 40.0 38.0 41.0 34.0 41.0 26-27 38.1860431988339 40.0 38.0 41.0 33.5 41.0 28-29 37.984598318706844 40.0 37.5 41.0 33.0 41.0 30-31 37.75639329088942 40.0 37.0 41.0 33.0 41.0 32-33 37.43912831354692 40.0 36.0 41.0 32.5 41.0 34-35 37.21638929545907 39.0 36.0 41.0 32.0 41.0 36-37 36.98598979257894 39.0 36.0 41.0 31.0 41.0 38-39 37.09507648538656 39.5 36.0 41.0 32.0 41.0 40-41 36.976070885760805 40.0 36.0 41.0 31.0 41.0 42-43 36.75473568923181 40.0 36.0 41.0 31.0 41.0 44-45 36.46786820701549 39.5 36.0 41.0 30.0 41.0 46-47 36.06823381900901 39.0 35.0 41.0 29.5 41.0 48-49 35.73600866546603 39.0 35.0 41.0 28.0 41.0 50-51 35.48139615193879 39.0 35.0 40.5 27.0 41.0 52-53 35.28140890117635 39.0 35.0 40.0 27.0 41.0 54-55 35.165268868369644 39.0 35.0 40.0 26.5 41.0 56-57 35.07754226823994 39.0 35.0 40.0 26.0 41.0 58-59 34.84366513475041 38.0 34.5 40.0 26.0 41.0 60-61 34.59110585079577 38.0 34.0 40.0 25.5 41.0 62-63 34.29791223705952 38.0 34.0 40.0 25.0 41.0 64-65 34.03758194145791 37.0 34.0 40.0 24.0 41.0 66-67 33.70972827058098 37.0 33.5 40.0 23.0 41.0 68-69 33.38479919448912 36.0 33.0 39.0 22.5 41.0 70-71 33.044509997610774 36.0 33.0 39.0 22.0 40.5 72-73 32.68046927232974 35.0 33.0 39.0 21.5 40.0 74-75 32.326294047611874 35.0 32.0 38.0 20.0 40.0 76-77 30.357223497145977 33.5 30.0 35.5 18.5 38.5 78-79 31.659866203277055 35.0 32.0 37.0 20.0 39.0 80-81 31.68064876553249 35.0 32.0 36.5 20.0 39.0 82-83 31.425160971672597 35.0 32.0 36.0 19.0 38.5 84-85 31.166234196466753 35.0 32.0 36.0 18.0 37.0 86-87 30.899014594363432 35.0 32.0 35.0 17.5 37.0 88-89 30.63634496425194 35.0 32.0 35.0 13.0 36.5 90-91 30.374884403566575 35.0 31.5 35.0 7.5 36.0 92-93 30.12092099688999 34.0 31.0 35.0 4.5 36.0 94-95 29.861961394907134 34.0 31.0 35.0 2.0 36.0 96-97 29.623193774356565 34.0 31.0 35.0 2.0 35.0 98-99 29.340454836578868 34.0 31.0 35.0 2.0 35.0 100-101 28.737547307702346 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34334005666983103 1101 2 0.38661782003909906 1101 3 0.35776111309110803 1101 4 0.30508046433671865 1101 5 0.2938075272965506 1101 6 0.294314833155525 1101 7 0.2912929746890143 1101 8 0.2751078292916489 1101 9 0.32604941091807405 1101 10-11 0.3373125413392515 1101 12-13 0.31643312243669186 1101 14-15 0.39209275370829744 1101 16-17 0.36388734527304933 1101 18-19 0.35424668287713956 1101 20-21 0.29476528186324913 1101 22-23 0.27875486102738734 1101 24-25 0.2935529815770579 1101 26-27 0.1911492282015388 1101 28-29 0.1890215774378845 1101 30-31 0.13310903640959282 1101 32-33 0.12716052439768077 1101 34-35 0.11830736932888186 1101 36-37 0.08749780904094706 1101 38-39 0.23420981551388564 1101 40-41 0.32070838763807785 1101 42-43 0.38116084687666785 1101 44-45 0.23177773495005027 1101 46-47 0.26118752414271285 1101 48-49 0.19263539242584216 1101 50-51 0.19796879768246356 1101 52-53 0.18562990996081652 1101 54-55 0.1450420438969786 1101 56-57 0.16222843036494794 1101 58-59 0.13811298450402631 1101 60-61 0.10527741515544875 1101 62-63 0.10388018541038235 1101 64-65 0.0852454133946523 1101 66-67 0.04294101282412299 1101 68-69 0.0538946201480357 1101 70-71 0.020555902609949328 1101 72-73 -0.09500018856501669 1101 74-75 -0.02573788302780855 1101 76-77 -0.21916295901536742 1101 78-79 -0.20393555614814218 1101 80-81 -0.10761019832578356 1101 82-83 -0.13555375702913253 1101 84-85 -0.16077149367571408 1101 86-87 -0.1405146549839209 1101 88-89 -0.23681712863740145 1101 90-91 -0.234311603335982 1101 92-93 -0.30070857850507693 1101 94-95 -0.31042640408032796 1101 96-97 -0.41844895129402104 1101 98-99 -0.5069222792064174 1101 100-101 -0.4834820258376027 1102 1 0.354433593832276 1102 2 0.36105073740782956 1102 3 0.3520202420401404 1102 4 0.2843923258913037 1102 5 0.28582277144173673 1102 6 0.3282552639252856 1102 7 0.25601715335796627 1102 8 0.2822999498503691 1102 9 0.38434978771788053 1102 10-11 0.37332121784876904 1102 12-13 0.29617117017901506 1102 14-15 0.3651873539153101 1102 16-17 0.3957241528684392 1102 18-19 0.34982755926485254 1102 20-21 0.31092508715823897 1102 22-23 0.35631274208260066 1102 24-25 0.4045909133313259 1102 26-27 0.344033328368738 1102 28-29 0.363936092730448 1102 30-31 0.28455159219253545 1102 32-33 0.24492346115513186 1102 34-35 0.25703042469972814 1102 36-37 0.29740153249442614 1102 38-39 0.3333345252747435 1102 40-41 0.26933434873581064 1102 42-43 0.2670928292395942 1102 44-45 0.2299798124059791 1102 46-47 0.31210596021908543 1102 48-49 0.09055729092875708 1102 50-51 0.18104381548953796 1102 52-53 0.23853843433015243 1102 54-55 0.19313347003367198 1102 56-57 0.18329635977362102 1102 58-59 0.16798208369210954 1102 60-61 0.17598853602189735 1102 62-63 0.14379548615085014 1102 64-65 0.12940671642045487 1102 66-67 0.1360333750425724 1102 68-69 0.023198153802873378 1102 70-71 -0.06680804812847185 1102 72-73 -0.08389488044348781 1102 74-75 -0.05165340326330892 1102 76-77 -0.008765694526005063 1102 78-79 0.08987117632399944 1102 80-81 0.07825397728745642 1102 82-83 0.07470307367779228 1102 84-85 0.06227361255342956 1102 86-87 0.03495884099092805 1102 88-89 -0.0012144748994238341 1102 90-91 -0.035779982063900206 1102 92-93 -0.06371350853565261 1102 94-95 -0.08428437357912699 1102 96-97 -0.09607407960177738 1102 98-99 -0.18627489797582442 1102 100-101 -0.18158784826611907 1103 1 0.36220670156960466 1103 2 0.3561874970107439 1103 3 0.2927982830313809 1103 4 0.25324962037277743 1103 5 0.24355376804606266 1103 6 0.28016083905358613 1103 7 0.2565713686704356 1103 8 0.27731650378250094 1103 9 0.307771563908517 1103 10-11 0.3232589968880788 1103 12-13 0.2779681291609819 1103 14-15 0.3754497121667839 1103 16-17 0.37594197526914996 1103 18-19 0.374362208374329 1103 20-21 0.27580461239487875 1103 22-23 0.23537854333437025 1103 24-25 0.2441814113058811 1103 26-27 0.21717847492364228 1103 28-29 0.21703598557968462 1103 30-31 0.28382271396075964 1103 32-33 0.2744981152228334 1103 34-35 0.2633197733069679 1103 36-37 0.1873453288227509 1103 38-39 0.26216438220403404 1103 40-41 0.40939931913851524 1103 42-43 0.3960508698368912 1103 44-45 0.3702030858886687 1103 46-47 0.39545210944670117 1103 48-49 0.389344742495183 1103 50-51 0.45251043840909233 1103 52-53 0.4006328967841526 1103 54-55 0.2400169577305391 1103 56-57 0.3263463621184002 1103 58-59 0.3093179498747176 1103 60-61 0.27968257537830965 1103 62-63 0.2540713526021463 1103 64-65 0.2888317640844562 1103 66-67 0.28197422692948493 1103 68-69 0.2678085345453667 1103 70-71 0.1797886932058148 1103 72-73 0.26419117494869937 1103 74-75 0.2794936456483228 1103 76-77 0.2687520703226909 1103 78-79 0.3046608048616868 1103 80-81 0.243167751044151 1103 82-83 0.27929576271697343 1103 84-85 0.24975421872627024 1103 86-87 0.2686988708639113 1103 88-89 0.2654223484532956 1103 90-91 0.24496998428922367 1103 92-93 0.18711628971249894 1103 94-95 0.24467688541476562 1103 96-97 0.1772621803708212 1103 98-99 0.1775050853445599 1103 100-101 0.34462846457883956 1104 1 0.25860864994346855 1104 2 0.2669902593622524 1104 3 0.2701354137761811 1104 4 0.22479708659084707 1104 5 0.2040001114804042 1104 6 0.23858678397550648 1104 7 0.24404226050614142 1104 8 0.2328538702457834 1104 9 0.36383334686384927 1104 10-11 0.26171989997904177 1104 12-13 0.20819311108489558 1104 14-15 0.27180388786617016 1104 16-17 0.32701470458058424 1104 18-19 0.2899116085165687 1104 20-21 0.3135426675679014 1104 22-23 0.26994675867752704 1104 24-25 0.2196727305853443 1104 26-27 0.19032465738943927 1104 28-29 0.18283630515109905 1104 30-31 0.1603087718124101 1104 32-33 0.23166424816489695 1104 34-35 0.11092496170314092 1104 36-37 0.13034715349515835 1104 38-39 0.1563490670878025 1104 40-41 0.19451627088144363 1104 42-43 0.3267504416131004 1104 44-45 0.2643463518037237 1104 46-47 0.3092680468264959 1104 48-49 0.157452104617974 1104 50-51 0.13017605574193425 1104 52-53 0.10941319067381272 1104 54-55 0.13845067335449102 1104 56-57 0.05444178167196867 1104 58-59 0.062269226222319674 1104 60-61 0.043229395356569 1104 62-63 0.03834330143875064 1104 64-65 0.1088921594543848 1104 66-67 0.1466433910687286 1104 68-69 0.03882213762960163 1104 70-71 0.07104238165189258 1104 72-73 -0.038024338275285174 1104 74-75 0.0407798051272934 1104 76-77 -0.05261588972136977 1104 78-79 -0.03455577362699458 1104 80-81 -0.06989681467446829 1104 82-83 -0.04992319980999227 1104 84-85 -0.07978390762424326 1104 86-87 -0.1519689587573545 1104 88-89 -0.07638582270003624 1104 90-91 -0.08831776753230969 1104 92-93 -0.20818019377909636 1104 94-95 -0.2541817852317436 1104 96-97 -0.2790369914176374 1104 98-99 -0.2545086645542405 1104 100-101 -0.24458239957069594 1105 1 0.3381904768795039 1105 2 0.3229910206876738 1105 3 0.3170535847986429 1105 4 0.2489983780173617 1105 5 0.2612604832044454 1105 6 0.2485887595270384 1105 7 0.2066557330137826 1105 8 0.25149542887741916 1105 9 0.3668243130341793 1105 10-11 0.3443196252862961 1105 12-13 0.2709162047169258 1105 14-15 0.34687798675241055 1105 16-17 0.34061750753958364 1105 18-19 0.30957721893843626 1105 20-21 0.30971111050351396 1105 22-23 0.249227612379606 1105 24-25 0.26320435401444087 1105 26-27 0.31447715677620636 1105 28-29 0.3611477723640135 1105 30-31 0.33007723497465236 1105 32-33 0.4068019949871271 1105 34-35 0.41349944454992027 1105 36-37 0.37413833895484316 1105 38-39 0.4116989956821371 1105 40-41 0.5006592703465671 1105 42-43 0.5426089302502248 1105 44-45 0.5273891534310238 1105 46-47 0.5101126579208 1105 48-49 0.513987489761007 1105 50-51 0.5018369339483968 1105 52-53 0.5712981196654567 1105 54-55 0.5299537524195799 1105 56-57 0.4852146007840048 1105 58-59 0.505850309107231 1105 60-61 0.528793107521544 1105 62-63 0.5459726521669879 1105 64-65 0.5529247296720499 1105 66-67 0.5856072504107104 1105 68-69 0.5627239948050828 1105 70-71 0.4807856667396848 1105 72-73 0.4831148090214086 1105 74-75 0.4825740581882201 1105 76-77 0.5624200989228179 1105 78-79 0.6252535856504515 1105 80-81 0.5655401983669996 1105 82-83 0.6332711617963476 1105 84-85 0.5627617538416061 1105 86-87 0.6108904997530828 1105 88-89 0.586060183340944 1105 90-91 0.6719192169908617 1105 92-93 0.6267232947182642 1105 94-95 0.6622505398793379 1105 96-97 0.6425026369211331 1105 98-99 0.5524084590176059 1105 100-101 0.6142892951101686 1106 1 0.2886293820575574 1106 2 0.2463255602741441 1106 3 0.24300824234464358 1106 4 0.17805407176962973 1106 5 0.18865623333085324 1106 6 0.28605495040440587 1106 7 0.2321726568086646 1106 8 0.2294285157045337 1106 9 0.3003980738306993 1106 10-11 0.24678851836065974 1106 12-13 0.2701024191748047 1106 14-15 0.3244145297322305 1106 16-17 0.3272124258895417 1106 18-19 0.35727384532383866 1106 20-21 0.3020956085993163 1106 22-23 0.24543135803306626 1106 24-25 0.29081797372050033 1106 26-27 0.2983762101682643 1106 28-29 0.4145892584805466 1106 30-31 0.41115806469952076 1106 32-33 0.42064414552435636 1106 34-35 0.46870705464584006 1106 36-37 0.43565823849073837 1106 38-39 0.4834044919856524 1106 40-41 0.4954913383805106 1106 42-43 0.5506358562898086 1106 44-45 0.5062513876081383 1106 46-47 0.5607487348309945 1106 48-49 0.48553724960265754 1106 50-51 0.6539079771927305 1106 52-53 0.6432686513128232 1106 54-55 0.6150311267515889 1106 56-57 0.5616316474520673 1106 58-59 0.6382667080078619 1106 60-61 0.651516700471575 1106 62-63 0.5650571756859222 1106 64-65 0.5836349135829124 1106 66-67 0.6649646182587148 1106 68-69 0.6666569103505893 1106 70-71 0.5819757415242108 1106 72-73 0.5229436018089544 1106 74-75 0.5931692665454946 1106 76-77 0.5485225430576257 1106 78-79 0.6051357923401817 1106 80-81 0.6647039420068559 1106 82-83 0.6802385353400133 1106 84-85 0.5485307766277572 1106 86-87 0.5590907922298918 1106 88-89 0.5026029795243723 1106 90-91 0.5654085685617325 1106 92-93 0.5642328917125745 1106 94-95 0.5866716966729335 1106 96-97 0.6338057021654961 1106 98-99 0.7242307101772383 1106 100-101 0.8349111344340869 1107 1 0.28567443500175926 1107 2 0.2811132766822695 1107 3 0.2330799835378059 1107 4 0.21096224157696497 1107 5 0.1909013702921456 1107 6 0.16783760563758676 1107 7 0.19982386315000156 1107 8 0.22452678089813816 1107 9 0.30630140410701756 1107 10-11 0.2751355095596253 1107 12-13 0.26545281959928246 1107 14-15 0.31770358058333414 1107 16-17 0.3368497770431276 1107 18-19 0.2846067697658512 1107 20-21 0.256062286907877 1107 22-23 0.2770053901573917 1107 24-25 0.3186297452111049 1107 26-27 0.30295936999246464 1107 28-29 0.34211211436559097 1107 30-31 0.3414683753551486 1107 32-33 0.34250431965222106 1107 34-35 0.31404683844836967 1107 36-37 0.3087747230593152 1107 38-39 0.3746091893104122 1107 40-41 0.4298674488898442 1107 42-43 0.37317715044610367 1107 44-45 0.32822361251016474 1107 46-47 0.31333229092767567 1107 48-49 0.3406296714563837 1107 50-51 0.39393068043044366 1107 52-53 0.4232097767632865 1107 54-55 0.47071841525870894 1107 56-57 0.5394122254603531 1107 58-59 0.5149829219632451 1107 60-61 0.5703507175631941 1107 62-63 0.5764346301023693 1107 64-65 0.6152453139341247 1107 66-67 0.5075051171224132 1107 68-69 0.5873074688566433 1107 70-71 0.54078088232 1107 72-73 0.6476918100345799 1107 74-75 0.5562646750969193 1107 76-77 0.5802350263122129 1107 78-79 0.69652033111932 1107 80-81 0.6597229069114157 1107 82-83 0.7225392863038316 1107 84-85 0.6841664031717833 1107 86-87 0.702332720849558 1107 88-89 0.6101370717795369 1107 90-91 0.6925977602757882 1107 92-93 0.7349391283330355 1107 94-95 0.6726320269895503 1107 96-97 0.6905051417055503 1107 98-99 0.7663001622710119 1107 100-101 0.9046015488532753 1108 1 0.1368810020038964 1108 2 0.11191387322340773 1108 3 0.1034037617502861 1108 4 0.10377857163246063 1108 5 0.08689858842272713 1108 6 0.10644249209909873 1108 7 0.17393768022716927 1108 8 0.08265255232463886 1108 9 0.15715428407061438 1108 10-11 0.06622069541639775 1108 12-13 0.08336544706576632 1108 14-15 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-1.1711604908970088 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 18.0 4 37.0 5 116.0 6 316.0 7 874.0 8 2252.0 9 4449.0 10 7677.0 11 12036.0 12 18363.0 13 25919.0 14 28085.0 15 33428.0 16 42243.0 17 56433.0 18 47586.0 19 26031.0 20 20267.0 21 19088.0 22 19266.0 23 20991.0 24 24610.0 25 30001.0 26 37990.0 27 47856.0 28 60674.0 29 77373.0 30 97651.0 31 122314.0 32 157230.0 33 207051.0 34 290177.0 35 434255.0 36 666952.0 37 1032225.0 38 1057619.0 39 251129.0 40 105.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.5662261047594 21.681925230626085 15.475771505616102 22.276077158998415 2 15.131414096958757 22.071974576516205 39.36871359870473 23.42789772782031 3 16.452070543063048 27.873336018378236 31.731769635997054 23.942823802561662 4 11.893809574003523 19.095845442635788 38.772837015063224 30.23750796829747 5 11.63416994056684 37.343282964763155 34.93473515892713 16.08781193574288 6 27.47712973133922 39.247374760395 18.89628403686067 14.379211471405112 7 25.051328229622804 33.40743953147054 23.451047947171975 18.09018429173468 8 25.255040015548047 34.0640220660234 21.60291477745348 19.078023140975073 9 24.900892066767575 16.70998403631953 23.109369983743186 35.279753913169706 10-11 22.958789139535803 27.24583614377086 29.162227438590584 20.63314727810276 12-13 24.55999068402169 25.024454443059096 29.453971236916978 20.96158363600224 14-15 21.835119857954755 26.669583277659726 28.14068157344002 23.3546152909455 16-17 19.79633343974429 29.965988648159193 28.43966398229964 21.798013929796873 18-19 19.72123524325058 27.46000702312753 32.42456311749765 20.394194616124242 20-21 21.25707686482089 26.840406483791522 32.14243838073061 19.760078270656976 22-23 20.541832557336434 27.082954369776076 32.312470762083166 20.062742310804328 24-25 20.29750858470047 27.030307009327874 32.31537648702593 20.35680791894573 26-27 20.121088373771254 26.942875968347074 32.30094514830796 20.635090509573715 28-29 20.249875535093683 26.957196220041705 32.39731908766094 20.39560915720367 30-31 20.687521740418028 27.524882582919773 31.894559788220096 19.893035888442103 32-33 20.035250396582022 27.169259228657634 32.494747628664896 20.300742746095445 34-35 20.033512037427464 26.76284561171014 32.33024115013151 20.87340120073088 36-37 20.960518071743653 27.378609060573318 31.36452926065088 20.29634360703215 38-39 20.616178902540504 27.442631274976453 31.744882836119142 20.1963069863639 40-41 20.22716985064321 27.22197297689902 31.901628933356395 20.649228239101365 42-43 20.109781380894688 27.437635349452055 31.5060396022508 20.94654366740245 44-45 20.737794897355315 27.686914515718573 31.071732067234166 20.503558519691946 46-47 20.727393923392647 28.32375042853278 30.253836968311916 20.69501867976266 48-49 20.634201972055518 27.84652019138295 30.53821936758496 20.981058468976567 50-51 20.93132119794503 27.73025406011811 30.013105817808555 21.325318924128307 52-53 20.820349138437745 28.113985645631846 29.80481468553477 21.26085053039564 54-55 21.027919121874444 28.7867889651852 29.4831633071517 20.702128605788655 56-57 20.700111792053928 28.36368998906976 29.447197529010115 21.489000689866195 58-59 20.533069137482798 27.854655874631582 30.023279097089468 21.58899589079615 60-61 21.08301347770809 28.35621515822513 29.47016809186141 21.090603272205367 62-63 20.859982881377746 28.285451319445016 29.556136572312123 21.298429226865114 64-65 20.700053025345955 28.184507323059275 29.851221841704184 21.26421780989058 66-67 20.75918448481149 28.175852992022744 29.464521997184974 21.600440525980794 68-69 21.360693553758235 28.527461234673552 29.04628326630786 21.06556194526035 70-71 21.41205647607499 28.163889079351957 29.224855922080117 21.19919852249294 72-73 20.962073396103467 28.34487079472106 29.59944360781941 21.093612201356066 74-75 20.88352530693439 28.488992827530957 29.362117577493436 21.265364288041216 76-77 20.987573017444678 28.701524031318883 29.039246183195573 21.27165676804087 78-79 21.12283445753644 28.77876305198306 29.20292175686895 20.89548073361155 80-81 20.941893504620282 28.488632402851998 29.23383664133663 21.33563745119109 82-83 20.61869444974548 28.573399041878105 29.42054214869993 21.387364359676482 84-85 20.773929404149367 28.604114995981455 29.16213329917455 21.459822300694626 86-87 20.962598745149645 28.708850603295755 29.00932639511673 21.319224256437867 88-89 20.542587370233793 28.717167112384423 29.17181628872431 21.568429228657475 90-91 20.316723239207278 28.602599351969012 29.25800893301364 21.82266847581007 92-93 20.690345079178897 28.861202369233297 28.95097921309186 21.497473338495947 94-95 20.584223601624004 28.60218466964679 29.139161535069185 21.67443019366002 96-97 20.55929635155438 28.717347006998455 29.10719423434884 21.616162407098326 98-99 20.354025121351803 28.634016331358264 29.095320332321183 21.91663821496875 100-101 20.342807993030686 28.961836404561698 28.795303220096656 21.90005238231096 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3217.0 1 2363.0 2 4266.0 3 5815.5 4 3274.5 5 2017.5 6 3027.0 7 6086.5 8 9682.0 9 10996.5 10 9275.0 11 7473.0 12 7529.0 13 8081.5 14 8688.0 15 9228.0 16 9773.5 17 10472.5 18 11163.5 19 12157.0 20 13682.0 21 15940.5 22 19674.5 23 26359.0 24 33770.5 25 41004.5 26 51544.0 27 64934.5 28 82382.0 29 97089.5 30 104497.5 31 113313.5 32 124208.0 33 133670.5 34 143143.0 35 151197.0 36 155002.0 37 157928.0 38 160994.5 39 162131.0 40 164495.0 41 163416.5 42 157235.0 43 154138.0 44 151678.5 45 148431.0 46 147256.0 47 147454.0 48 148237.0 49 147957.5 50 145869.0 51 139084.5 52 128563.0 53 118686.0 54 112054.0 55 107863.5 56 100399.5 57 90402.0 58 82397.5 59 75812.0 60 67539.5 61 57052.5 62 46713.0 63 38408.5 64 32191.0 65 27074.5 66 21586.5 67 16298.0 68 11930.0 69 8649.5 70 6323.5 71 4635.0 72 3513.5 73 2746.5 74 2250.0 75 1830.5 76 1346.5 77 1052.0 78 865.5 79 616.0 80 396.0 81 244.0 82 154.0 83 116.5 84 79.5 85 54.0 86 40.0 87 31.5 88 22.5 89 18.0 90 15.5 91 7.0 92 4.0 93 3.5 94 2.0 95 1.5 96 2.5 97 2.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05023400372237581 2 0.001967598866823673 3 3.2124063131815073E-4 4 3.011630918607663E-4 5 0.0027506229056616653 6 0.0019475213273662886 7 0.0 8 2.007753945738442E-5 9 1.8069785511645975E-4 10-11 1.003876972869221E-5 12-13 0.0 14-15 4.718221772485339E-4 16-17 0.0 18-19 6.0232618372153255E-5 20-21 4.8186094697722604E-4 22-23 0.0 24-25 5.420935653493793E-4 26-27 0.007237952974387083 28-29 0.007438728368960927 30-31 9.536831242257599E-4 32-33 0.006434851396091706 34-35 0.007830240388379922 36-37 0.004447174989810649 38-39 0.0024795761229869755 40-41 0.0019977151760097495 42-43 0.001284962525272603 44-45 7.529077296519157E-4 46-47 6.525200323649936E-4 48-49 0.0020077539457384417 50-51 0.0015560093079472926 52-53 0.0023189558073279004 54-55 7.127526507371469E-4 56-57 2.8108555240338186E-4 58-59 8.332178874814533E-4 60-61 0.006384657547448245 62-63 0.0029915533791502785 64-65 0.0010641095912413742 66-67 0.005621711048067637 68-69 0.007418650829503543 70-71 0.002780739214847742 72-73 6.123649534502248E-4 74-75 0.003764538648259579 76-77 0.002339033346785285 78-79 0.002509692432173052 80-81 8.432566572101457E-4 82-83 1.7065908538776756E-4 84-85 5.822486442641482E-4 86-87 0.005129811331361719 88-89 0.0029413595305068175 90-91 0.001284962525272603 92-93 1.4054277620169093E-4 94-95 5.722098745354559E-4 96-97 4.2162832860507285E-4 98-99 5.320547956206871E-4 100-101 2.007753945738442E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4980690.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.60541806751258 #Duplication Level Percentage of deduplicated Percentage of total 1 73.90689882978744 40.357171086738994 2 16.110083818378097 17.593957240104107 3 4.814377991582903 7.88673368896248 4 1.8376924519233087 4.013918584671381 5 0.8668703106302976 2.3667907861140947 6 0.5355219105265563 1.7545438685169403 7 0.3315534309710785 1.2673229596925737 8 0.24985914694268238 1.0914930541437784 9 0.18487703366894942 0.9085758943117124 >10 1.0165856733592802 10.342163516411391 >50 0.08966574289339731 3.381405921610826 >100 0.051139106483584475 5.154318572441136 >500 0.0035820587786669265 1.3402415911231187 >1k 0.0011078520631858499 0.9992317037503942 >5k 7.385680421206868E-5 0.2746258868956269 >10k+ 1.1078520631810301E-4 1.2675056445112285 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26472 0.5314926245158803 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21511 0.4318879512677962 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14874 0.2986333218891358 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8570 0.17206451314978446 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5049 0.10137149672033394 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.0077539457384417E-5 0.0 5 0.0 0.0 0.0 2.0077539457384417E-5 0.0 6 0.0 0.0 0.0 2.0077539457384417E-5 0.0 7 0.0 0.0 0.0 2.0077539457384417E-5 0.0 8 0.0 0.0 0.0 2.0077539457384417E-5 0.0 9 0.0 0.0 0.0 2.0077539457384417E-5 0.0 10-11 0.0 0.0 0.0 2.0077539457384417E-5 0.0 12-13 0.0 0.0 0.0 2.0077539457384417E-5 0.0 14-15 0.0 0.0 0.0 2.0077539457384417E-5 0.0 16-17 0.0 0.0 0.0 4.0155078914768835E-5 0.0 18-19 0.0 0.0 0.0 5.0193848643461045E-5 0.0 20-21 0.0 0.0 0.0 8.031015782953767E-5 0.0 22-23 0.0 0.0 0.0 2.0077539457384418E-4 0.0 24-25 0.0 0.0 0.0 3.714344799616117E-4 0.0 26-27 0.0 0.0 0.0 6.22403723178917E-4 0.0 28-29 0.0 0.0 0.0 0.001104264670156143 0.0 30-31 0.0 0.0 0.0 0.002108141643025364 0.0 32-33 0.0 0.0 0.0 0.004587717766012339 0.0 34-35 0.0 2.0077539457384417E-5 0.0 0.008251868716984996 0.0 36-37 0.0 2.0077539457384417E-5 0.0 0.013682843140207481 0.0 38-39 0.0 2.0077539457384417E-5 0.0 0.022767929744673933 0.0 40-41 0.0 2.0077539457384417E-5 0.0 0.03572798146441557 0.0 42-43 0.0 2.0077539457384417E-5 0.0 0.04963167753865429 0.0 44-45 0.0 2.0077539457384417E-5 0.0 0.06430835888200229 0.0 46-47 0.0 2.0077539457384417E-5 0.0 0.08163527543372505 0.0 48-49 0.0 2.0077539457384417E-5 0.0 0.10275684694289346 0.0 50-51 0.0 2.0077539457384417E-5 0.0 0.12857656268508982 0.0 52-53 0.0 2.0077539457384417E-5 0.0 0.15362329315817688 0.0 54-55 0.0 2.0077539457384417E-5 0.0 0.18215347672712015 0.0 56-57 0.0 4.0155078914768835E-5 0.0 0.21603432456145633 0.0 58-59 0.0 4.0155078914768835E-5 0.0 0.2539106027478121 0.0 60-61 0.0 4.0155078914768835E-5 0.0 0.29413595305068174 0.0 62-63 0.0 4.0155078914768835E-5 0.0 0.33834669493584224 0.0 64-65 0.0 5.0193848643461045E-5 0.0 0.38957453686135857 0.0 66-67 0.0 6.0232618372153255E-5 0.0 0.44977703892432574 0.0 68-69 0.0 1.0038769728692209E-4 0.0 0.5164043536136559 0.0 70-71 0.0 1.1042646701561431E-4 0.0 0.5919862509009797 0.0 72-73 0.0 1.2046523674430651E-4 0.0 0.6813513790257977 0.0 74-75 0.0 1.3050400647299871E-4 0.0 0.772503408162323 0.0 76-77 0.0 1.4054277620169093E-4 0.0 0.8707930025759483 0.0 78-79 0.0 1.4054277620169093E-4 0.0 0.9823940056498197 0.0 80-81 0.0 1.4054277620169093E-4 0.0 1.1065635484240137 0.0 82-83 0.0 1.4054277620169093E-4 0.0 1.2461024476528353 0.0 84-85 0.0 1.4054277620169093E-4 0.0 1.3873077826566198 0.0 86-87 0.0 1.4054277620169093E-4 0.0 1.5418546426298363 0.0 88-89 0.0 1.4054277620169093E-4 0.0 1.7170612907047016 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11300 0.0 55.763332 1 TATAACG 710 0.0 47.4998 2 GTATCAA 21855 0.0 43.81349 1 ATAACGC 880 0.0 40.481567 3 GTACCTG 4455 0.0 39.57399 1 TACCTGG 4535 0.0 37.287605 2 ATCAACG 28570 0.0 33.051018 3 TCAACGC 28890 0.0 32.767136 4 TATCAAC 29125 0.0 32.552654 2 CAACGCA 29520 0.0 32.1322 5 AACGCAG 31595 0.0 30.36738 6 TCTGTCG 1320 0.0 29.505377 8 GTATAAC 1540 0.0 28.08047 1 CGCAGAG 35135 0.0 27.13118 8 ACGCAGA 35440 0.0 27.058508 7 ACCTGGG 6170 0.0 26.251125 3 GTGGTAT 4935 0.0 26.191805 1 GTCTCGC 1400 0.0 26.136435 1 GTCGTAT 440 0.0 25.907423 9 TGGTATC 4970 0.0 25.135708 2 >>END_MODULE