FastQCFastQC Report
Wed 25 May 2016
SRR2049395_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049395_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11305438
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265310.23467467602758954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236720.20938596098620857No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117270.10372884270383864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA191450.054.787721
GTATCAA332800.042.523281
GTGGTAT76200.036.5950781
ATCAACG412150.033.9765323
TCAACGC412050.033.8828244
CAACGCA424600.032.981015
TATCAAC436000.032.1714322
AACGCAG448650.031.2450986
TGGTATC92600.028.6743952
CGCAGAG519100.026.584768
ACGCAGA523800.026.554817
GCAGAGT572650.023.6093089
GTACATG500500.022.6847171
TACATGG501100.021.9062982
GAGTACT273400.021.78771412-13
CAGAGTA551350.021.11298410-11
ACATGGG532750.020.0527023
AGAGTAC528600.019.74354710-11
GTACTTT311900.019.09678314-15
AGTACTT285250.018.3180712-13
Produced by FastQC (version 0.11.5)