##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049395_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11305438 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.625177724206704 31.0 31.0 34.0 30.0 34.0 2 31.679496981894907 33.0 31.0 34.0 30.0 34.0 3 31.70541247495232 33.0 31.0 34.0 30.0 34.0 4 35.33486929033621 37.0 35.0 37.0 33.0 37.0 5 35.342432464801455 37.0 35.0 37.0 33.0 37.0 6 35.43112553445519 37.0 35.0 37.0 33.0 37.0 7 35.3821279635517 37.0 35.0 37.0 33.0 37.0 8 35.35172179972152 37.0 35.0 37.0 33.0 37.0 9 36.961208579446456 39.0 37.0 39.0 33.0 39.0 10-11 36.889682469622144 39.0 37.0 39.0 33.0 39.0 12-13 36.85263605001416 39.0 37.0 39.0 33.0 39.0 14-15 38.05095428412416 40.0 38.0 41.0 33.0 41.0 16-17 38.021404920357796 40.0 38.0 41.0 33.0 41.0 18-19 38.01079520315798 40.0 38.0 41.0 33.0 41.0 20-21 37.955705298635934 40.0 38.0 41.0 33.0 41.0 22-23 37.86007180792111 40.0 38.0 41.0 33.0 41.0 24-25 37.74135995438655 40.0 38.0 41.0 33.0 41.0 26-27 37.601797559723025 40.0 37.5 41.0 32.5 41.0 28-29 37.44571293920678 40.0 37.0 41.0 32.0 41.0 30-31 37.28161323780645 40.0 37.0 41.0 32.0 41.0 32-33 37.07625582485172 40.0 36.5 41.0 31.0 41.0 34-35 36.90328574620462 39.0 36.0 41.0 31.0 41.0 36-37 36.737801887905626 39.0 36.0 41.0 30.5 41.0 38-39 36.54948003783666 39.0 36.0 40.0 30.0 41.0 40-41 36.34415088561805 39.0 35.0 40.0 30.0 41.0 42-43 36.10243216583028 39.0 35.0 40.0 30.0 41.0 44-45 35.82816831156829 38.5 35.0 40.0 29.0 41.0 46-47 35.49972062117364 38.0 35.0 40.0 27.5 41.0 48-49 35.22832034459877 38.0 34.0 40.0 27.0 41.0 50-51 33.82480961816782 36.5 32.5 39.0 25.0 40.0 52-53 34.15132257591435 37.5 33.5 39.0 26.5 39.5 54-55 35.254812020551526 38.0 35.0 40.0 28.0 41.0 56-57 35.341668319263704 38.0 35.0 40.0 28.0 41.0 58-59 35.14432811006526 38.0 34.5 40.0 28.0 41.0 60-61 34.848542090983116 38.0 34.0 40.0 27.0 41.0 62-63 34.52753002581589 37.0 34.0 40.0 26.5 41.0 64-65 34.20718290613774 37.0 34.0 40.0 26.0 41.0 66-67 33.843870622261605 36.0 33.5 39.0 26.0 41.0 68-69 33.4759725806289 35.5 33.0 39.0 26.0 41.0 70-71 33.1156255069463 35.0 33.0 38.5 25.0 40.0 72-73 32.852002284210485 35.0 33.0 38.0 25.0 40.0 74-75 32.65669861707259 35.0 33.0 37.0 25.0 39.5 76-77 32.333909486744346 35.0 33.0 37.0 24.0 39.0 78-79 31.982306656318844 35.0 32.0 36.0 23.5 39.0 80-81 31.66299390611845 35.0 32.0 36.0 22.5 38.0 82-83 31.355056168544728 35.0 32.0 35.5 21.0 37.0 84-85 31.07303631225964 35.0 32.0 35.0 20.0 37.0 86-87 30.803468383975925 35.0 31.5 35.0 19.5 36.0 88-89 30.534385266630096 34.0 31.0 35.0 18.0 36.0 90-91 30.2797517884756 34.0 31.0 35.0 16.0 36.0 92-93 30.030361893099588 34.0 31.0 35.0 9.0 35.5 94-95 29.778437022961867 34.0 31.0 35.0 4.5 35.0 96-97 29.511896841148484 34.0 31.0 35.0 2.0 35.0 98-99 29.15873445150909 34.0 30.0 35.0 2.0 35.0 100-101 28.550419541463143 34.0 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23143681011370631 1101 2 0.21778344193801402 1101 3 0.2542044296257764 1101 4 0.17028896084877942 1101 5 0.18980416467699257 1101 6 0.18139509061038694 1101 7 0.18523441016692033 1101 8 0.20230155424409446 1101 9 0.2629939595617685 1101 10-11 0.26414338484512 1101 12-13 0.20981204980032686 1101 14-15 0.2714958204460842 1101 16-17 0.3081216588987701 1101 18-19 0.2702680360995089 1101 20-21 0.19286457975422877 1101 22-23 0.22693762322779776 1101 24-25 0.17002358787701155 1101 26-27 0.17378354661476436 1101 28-29 0.20531805796098723 1101 30-31 0.1080229503399579 1101 32-33 0.0749754093198689 1101 34-35 0.06745038583600405 1101 36-37 -0.023595819827733067 1101 38-39 -0.04882017470993816 1101 40-41 -0.17169589881677183 1101 42-43 -0.19738907003211636 1101 44-45 -0.24210061964706142 1101 46-47 -0.3336353794228799 1101 48-49 -0.4366954089568722 1101 50-51 -0.45240074753190385 1101 52-53 -0.3201835487276199 1101 54-55 0.029321406139324324 1101 56-57 0.026952931651756273 1101 58-59 -0.004751587730488893 1101 60-61 -6.177225523344987E-4 1101 62-63 -0.07415804311830243 1101 64-65 -0.06158477352533254 1101 66-67 -0.13079981339927826 1101 68-69 -0.19861796618850036 1101 70-71 -0.34399860315897257 1101 72-73 -0.28552566428859905 1101 74-75 -0.33304231353986324 1101 76-77 -0.3483993219614696 1101 78-79 -0.4037555735941005 1101 80-81 -0.45046301187500504 1101 82-83 -0.4275611511139523 1101 84-85 -0.4896155334565684 1101 86-87 -0.5907121675401079 1101 88-89 -0.6213617022017885 1101 90-91 -0.6835583495018156 1101 92-93 -0.7911765698295561 1101 94-95 -0.8465981571046406 1101 96-97 -0.9969254514393526 1101 98-99 -1.167240768658921 1101 100-101 -1.3117604554545537 1102 1 0.281795459203078 1102 2 0.2619440441086027 1102 3 0.2812963610359489 1102 4 0.29381272617725074 1102 5 0.270641930541089 1102 6 0.3154294791252923 1102 7 0.3081137489050647 1102 8 0.3389647100672093 1102 9 0.3806766043187082 1102 10-11 0.3847764574755175 1102 12-13 0.3297579016349843 1102 14-15 0.4337342329476854 1102 16-17 0.3582942518386929 1102 18-19 0.336632577370402 1102 20-21 0.3393065927788115 1102 22-23 0.31748365824602587 1102 24-25 0.322924766305249 1102 26-27 0.324046569395378 1102 28-29 0.33750868070845996 1102 30-31 0.22655231276479526 1102 32-33 0.20024572046969524 1102 34-35 0.2187881213201237 1102 36-37 0.13404812415272715 1102 38-39 0.13837041051645826 1102 40-41 0.1136164375580293 1102 42-43 -0.007446741144590874 1102 44-45 0.015925611704062703 1102 46-47 -0.017031889381343035 1102 48-49 -0.08637737542948543 1102 50-51 -0.19457113471637655 1102 52-53 0.04244983569086713 1102 54-55 0.14449957116156753 1102 56-57 0.09028828708895986 1102 58-59 0.13895717186048984 1102 60-61 0.11060856079573966 1102 62-63 0.14115337419173102 1102 64-65 0.0829350425681099 1102 66-67 0.05947975738860123 1102 68-69 -0.002900174587360027 1102 70-71 -0.011547144343360571 1102 72-73 -0.02676538744595547 1102 74-75 -0.08040316539912595 1102 76-77 -0.06528961023875723 1102 78-79 -0.06746981933200757 1102 80-81 -0.16470476770668796 1102 82-83 -0.26273884328986696 1102 84-85 -0.267766890755464 1102 86-87 -0.2945507153758129 1102 88-89 -0.36843768432819957 1102 90-91 -0.44734548296624155 1102 92-93 -0.6202894673744908 1102 94-95 -0.7029230051059336 1102 96-97 -0.8391297689780863 1102 98-99 -0.9151542979781766 1102 100-101 -1.027677450914826 1103 1 0.1464566616817855 1103 2 0.15741807799999918 1103 3 0.19957420975694617 1103 4 0.19893623197029342 1103 5 0.16714450062462305 1103 6 0.15485351556537807 1103 7 0.1594593173722103 1103 8 0.14456378167659523 1103 9 0.24866089661540514 1103 10-11 0.23839652647364318 1103 12-13 0.18243060209805861 1103 14-15 0.24099382457307428 1103 16-17 0.2723691343105443 1103 18-19 0.27896286611407817 1103 20-21 0.2563758026033298 1103 22-23 0.2090512951112018 1103 24-25 0.23979553750975668 1103 26-27 0.2401435463942292 1103 28-29 0.21932616250538928 1103 30-31 0.19598285669522397 1103 32-33 0.09167472218756956 1103 34-35 0.15958077665077752 1103 36-37 0.19133532600845626 1103 38-39 0.13310707837973013 1103 40-41 0.12667642845078575 1103 42-43 0.02499667954133855 1103 44-45 0.047786881570445416 1103 46-47 0.04044886641574408 1103 48-49 0.08769297508160179 1103 50-51 -0.013824890407725832 1103 52-53 0.07743635622094303 1103 54-55 0.2568283144439718 1103 56-57 0.2823544357708485 1103 58-59 0.30541683334877234 1103 60-61 0.2804247429510127 1103 62-63 0.25052457797843886 1103 64-65 0.16625350141679718 1103 66-67 0.17470777983476182 1103 68-69 0.14776442017110725 1103 70-71 0.11544149032823015 1103 72-73 0.16967678356668614 1103 74-75 0.09962196420342195 1103 76-77 0.022987748854113477 1103 78-79 0.055276636297978854 1103 80-81 -1.9057355761376016E-4 1103 82-83 -0.011958543914371234 1103 84-85 -0.07318033297342552 1103 86-87 -0.15465564198612824 1103 88-89 -0.19570520591805263 1103 90-91 -0.2703103452256528 1103 92-93 -0.23533494284323453 1103 94-95 -0.30232259572113307 1103 96-97 -0.41393905186316715 1103 98-99 -0.4675251964627982 1103 100-101 -0.4996529046890963 1104 1 0.07088529130991361 1104 2 0.10269467260483722 1104 3 0.12812846740971295 1104 4 0.10122061103079716 1104 5 0.09022199664119768 1104 6 0.02588645822500979 1104 7 0.018328185366918603 1104 8 0.03980743330292569 1104 9 0.07951217479522654 1104 10-11 0.07823583042856797 1104 12-13 0.050469168547557786 1104 14-15 0.13811163625367584 1104 16-17 0.13530368016778738 1104 18-19 0.012366431335642858 1104 20-21 0.05932353749624042 1104 22-23 0.060091097873346655 1104 24-25 0.10487545412801325 1104 26-27 0.07336400623656658 1104 28-29 0.007092042727059322 1104 30-31 0.025658934034034075 1104 32-33 0.00954879696318045 1104 34-35 -0.024803303993671477 1104 36-37 -0.019633439911530104 1104 38-39 -0.07686616635491106 1104 40-41 -0.11327168094841511 1104 42-43 -0.14998984875525423 1104 44-45 -0.07880606279657343 1104 46-47 -0.07370932784893114 1104 48-49 -0.1264183341474947 1104 50-51 -0.16134480322306644 1104 52-53 -0.1397741053374446 1104 54-55 0.002696582071514797 1104 56-57 -0.004260979884897154 1104 58-59 0.021729636894868065 1104 60-61 -0.04965100972760439 1104 62-63 -0.04594136452124786 1104 64-65 -0.018214047073016104 1104 66-67 -0.019615625414616034 1104 68-69 -0.06641293217918331 1104 70-71 -0.10467589354691142 1104 72-73 -0.17391377878360714 1104 74-75 -0.14412923360399787 1104 76-77 -0.16602138885345852 1104 78-79 -0.16943384792394411 1104 80-81 -0.1586198783994952 1104 82-83 -0.17617341522629104 1104 84-85 -0.16445611752220302 1104 86-87 -0.19474686873089908 1104 88-89 -0.23886289939843408 1104 90-91 -0.29163228547711384 1104 92-93 -0.3252348005618195 1104 94-95 -0.3252029213033545 1104 96-97 -0.34479009315785447 1104 98-99 -0.3670169616748211 1104 100-101 -0.48789623829712525 1105 1 0.24147128996832734 1105 2 0.25822526431890225 1105 3 0.22161059660167837 1105 4 0.23460567716129788 1105 5 0.2642384372661368 1105 6 0.26902163855118033 1105 7 0.294843241380498 1105 8 0.2870156910434005 1105 9 0.3772211948857347 1105 10-11 0.3222284547911656 1105 12-13 0.2542315754524864 1105 14-15 0.3519492732159719 1105 16-17 0.3663454905777712 1105 18-19 0.36002616441074764 1105 20-21 0.38364401705531037 1105 22-23 0.3096200814378065 1105 24-25 0.37082690052162803 1105 26-27 0.42964357385904606 1105 28-29 0.37489055446072683 1105 30-31 0.43922340099446444 1105 32-33 0.3999387640674925 1105 34-35 0.4623936572830232 1105 36-37 0.42511132670732366 1105 38-39 0.32820730238447027 1105 40-41 0.37596511114941933 1105 42-43 0.40557318618738236 1105 44-45 0.287234341695779 1105 46-47 0.31401629810844867 1105 48-49 0.20902452664956428 1105 50-51 0.155716572375745 1105 52-53 0.2788559337275913 1105 54-55 0.43033156643922865 1105 56-57 0.4356303007506952 1105 58-59 0.4327593462336097 1105 60-61 0.4972006551025814 1105 62-63 0.48935696285402486 1105 64-65 0.4680711835773721 1105 66-67 0.4784442319351925 1105 68-69 0.44638386262455043 1105 70-71 0.45827369085662184 1105 72-73 0.44505740614898315 1105 74-75 0.4294022778897215 1105 76-77 0.4213806570688874 1105 78-79 0.47567213536082065 1105 80-81 0.42895638323624397 1105 82-83 0.39809492316550177 1105 84-85 0.3716724810025305 1105 86-87 0.3980989725049895 1105 88-89 0.37153935668654725 1105 90-91 0.33214491981313543 1105 92-93 0.30765819439960396 1105 94-95 0.28202930273107896 1105 96-97 0.24607751307963 1105 98-99 0.20981927002087986 1105 100-101 0.20376141315580298 1106 1 0.2724608907146333 1106 2 0.2985206050090454 1106 3 0.2693819178848287 1106 4 0.26254979796278377 1106 5 0.2880813420819166 1106 6 0.3462036216615232 1106 7 0.3478894356407025 1106 8 0.3233754632754753 1106 9 0.3513393835940093 1106 10-11 0.39212930339784435 1106 12-13 0.37366973547621996 1106 14-15 0.35991866531738737 1106 16-17 0.3817432397920939 1106 18-19 0.41168567000435274 1106 20-21 0.454347148613941 1106 22-23 0.4864766018411544 1106 24-25 0.488069380253485 1106 26-27 0.5025568634831572 1106 28-29 0.46873410584237973 1106 30-31 0.49069384866254495 1106 32-33 0.5112262468466042 1106 34-35 0.5594752076507987 1106 36-37 0.5449043173735078 1106 38-39 0.5206344620986201 1106 40-41 0.5292517535401657 1106 42-43 0.5349774449504068 1106 44-45 0.5401787791525337 1106 46-47 0.5603800646153942 1106 48-49 0.5155967583681687 1106 50-51 0.45240736645585145 1106 52-53 0.5526683807246542 1106 54-55 0.6084850773803296 1106 56-57 0.6011271372908951 1106 58-59 0.617153720197301 1106 60-61 0.5590610077711275 1106 62-63 0.5943593797437927 1106 64-65 0.6448544682439845 1106 66-67 0.6352202276392873 1106 68-69 0.665790463900187 1106 70-71 0.6630345439583252 1106 72-73 0.6672955388667177 1106 74-75 0.64953222658567 1106 76-77 0.6546458162404392 1106 78-79 0.6629942376926117 1106 80-81 0.6360404071971821 1106 82-83 0.611641895955831 1106 84-85 0.5831829700979512 1106 86-87 0.5404064291622497 1106 88-89 0.629092516479048 1106 90-91 0.5615806526373852 1106 92-93 0.5070600325908217 1106 94-95 0.5485846110664205 1106 96-97 0.573905167335667 1106 98-99 0.6064895404209167 1106 100-101 0.6105055334565286 1107 1 0.210942828350845 1107 2 0.1976677726756897 1107 3 0.20285148767614558 1107 4 0.20594633037357113 1107 5 0.24976660536596285 1107 6 0.21018003987336442 1107 7 0.19993865155449697 1107 8 0.2772863143281583 1107 9 0.3213056850737743 1107 10-11 0.2725094415072391 1107 12-13 0.3009041999632487 1107 14-15 0.3919242762358195 1107 16-17 0.33626801602631673 1107 18-19 0.3357372532822538 1107 20-21 0.35722442170327895 1107 22-23 0.38294624052212356 1107 24-25 0.33463646908453626 1107 26-27 0.4248033406028213 1107 28-29 0.4486490594172139 1107 30-31 0.4250197849697628 1107 32-33 0.42564547839691613 1107 34-35 0.44343602881541955 1107 36-37 0.4699484498052442 1107 38-39 0.49660191317440905 1107 40-41 0.40017276868656637 1107 42-43 0.378900714080018 1107 44-45 0.44465974587524215 1107 46-47 0.4806295671388341 1107 48-49 0.3876086877591476 1107 50-51 0.39688562782462355 1107 52-53 0.5079894244315497 1107 54-55 0.5707844811260614 1107 56-57 0.5071151727374925 1107 58-59 0.5299415766743252 1107 60-61 0.6145928466296269 1107 62-63 0.5923955762314321 1107 64-65 0.6408150769988481 1107 66-67 0.6860511372768769 1107 68-69 0.6461305060807447 1107 70-71 0.6558014536762826 1107 72-73 0.6327649428923152 1107 74-75 0.6761796938505356 1107 76-77 0.6835010173731035 1107 78-79 0.6676468544587699 1107 80-81 0.6067305343775153 1107 82-83 0.7035434153313993 1107 84-85 0.6826465278321407 1107 86-87 0.6826492752233015 1107 88-89 0.7287306664188051 1107 90-91 0.7341320202763306 1107 92-93 0.737386171261381 1107 94-95 0.7198464146979084 1107 96-97 0.7312068781670575 1107 98-99 0.8062787273292997 1107 100-101 0.8393443194773802 1108 1 0.16522015679396773 1108 2 0.15425128328049453 1108 3 0.14588324070409442 1108 4 0.19708850463200633 1108 5 0.13200549913161552 1108 6 0.18793842719064457 1108 7 0.2192735402400814 1108 8 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-0.6720485197275181 2316 90-91 -0.5911519451561418 2316 92-93 -0.7231740927452179 2316 94-95 -0.9912630297048644 2316 96-97 -0.7699925268180685 2316 98-99 -0.45821663505476096 2316 100-101 -0.3835739618129814 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 79561.0 3 18117.0 4 14978.0 5 13761.0 6 14291.0 7 14662.0 8 16365.0 9 19617.0 10 22992.0 11 27083.0 12 29989.0 13 32300.0 14 36431.0 15 45653.0 16 59657.0 17 71710.0 18 57305.0 19 41746.0 20 41058.0 21 42581.0 22 46183.0 23 50446.0 24 59266.0 25 71533.0 26 88070.0 27 108396.0 28 136508.0 29 172060.0 30 219232.0 31 285262.0 32 381345.0 33 528623.0 34 767160.0 35 1171182.0 36 1783714.0 37 2398319.0 38 1969746.0 39 368413.0 40 123.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.427506510811185 18.73576983359093 14.494743447189999 23.341980208407882 2 16.515020236773122 19.839450362961596 38.46800263425244 25.177526766012836 3 19.073351908034883 24.304594204572815 29.269582207207407 27.352471680184898 4 13.985660958177057 16.631059373009695 36.11079364517727 33.27248602363598 5 14.95518414174071 36.26084572075558 33.61485694572827 15.16911319177543 6 32.361633095430555 35.30317433105657 17.204650347318236 15.13054222619464 7 28.963476390256243 31.49492848038396 21.03471914475436 18.506875984605436 8 26.967860102045265 33.580245100766625 19.686000412384523 19.765894384803588 9 26.57620372146981 16.276262026731956 20.10915849079337 37.038375761004865 10-11 25.38554298719386 25.775159546010396 27.25882421419432 21.580473252601422 12-13 26.175576195280886 23.56256149146696 27.090997375007674 23.170864938244478 14-15 23.70404622539013 24.914205320324978 25.140927157062876 26.240821297222016 16-17 23.1060642918217 27.94909244472641 25.673114330716796 23.271728932735098 18-19 23.060137126777295 26.815267704689944 27.815978628242178 22.308616540290576 20-21 24.13425882620384 25.726233937053127 27.64712035100266 22.492386885740373 22-23 24.019299251351732 25.791617018163766 27.41879058767876 22.770293142805738 24-25 23.56616178704828 25.88450795799083 27.494744430954448 23.05458582400644 26-27 23.30981020630273 26.05444972494385 27.592012367973755 23.043727700779666 28-29 23.4064023904423 25.815711854439254 27.726372691243544 23.051513063874904 30-31 23.45135721821326 25.9461501780526 27.46435829991189 23.13813430382225 32-33 23.2695295911393 25.992386928848283 27.664183493519364 23.073899986493053 34-35 23.717574118054888 25.879375504661585 27.308966752495717 23.094083624787814 36-37 23.715652535268053 25.789596077016796 27.27246150778831 23.222289879926837 38-39 23.520276728962262 26.01444457462037 27.442210622559227 23.023068073858134 40-41 23.602187871968507 25.963180201077552 27.293800550289486 23.140831376664448 42-43 23.555228762144644 25.975690255868184 27.135738065989912 23.333342915997264 44-45 23.528923936912143 26.240161541090824 26.904036368860297 23.326878153136736 46-47 23.77697607356582 26.11270960969756 26.86300354751239 23.247310769224235 48-49 23.91854429475295 26.070064570111036 26.54011088256109 23.471280252574925 50-51 23.634888072458622 26.336243580032466 26.495649756310808 23.533218591198104 52-53 23.64261063807605 26.443667141119626 26.48709464803527 23.42662757276905 54-55 23.694742684225258 26.48735775103596 26.475689806409424 23.342209758329354 56-57 23.417543315244256 26.28298201931287 26.692896537009602 23.606578128433277 58-59 23.604196846964335 26.06432612408925 26.695412706802994 23.63606432214342 60-61 23.66280707454245 26.13900042338562 26.588382710705556 23.609809791366374 62-63 23.45948324664756 26.302091073134488 26.918028871669677 23.32039680854828 64-65 23.75189050539496 26.155280350296124 26.627851414634957 23.46497772967396 66-67 23.442226200443717 26.099635799163806 26.726570562203722 23.73156743818875 68-69 23.437870612036416 26.19115192241377 26.7552550920919 23.615722373457917 70-71 23.5634448407869 26.10511634115286 26.65765516218961 23.673783655870626 72-73 23.49442763250684 26.239102063117603 26.588049217828797 23.67842108654676 74-75 23.385888623520973 26.367839030094686 26.546047977542507 23.700224368841837 76-77 23.530828175488907 26.41054011937231 26.37267897000689 23.68595273513189 78-79 23.5544207419489 26.362314823378156 26.36708719728653 23.716177237386415 80-81 23.422251423487985 26.407223721603224 26.44147731354174 23.729047541367052 82-83 23.456567209112286 26.48423084759189 26.52374009426839 23.535461849027435 84-85 23.40618037318535 26.29686611385256 26.258885539277717 24.038067973684377 86-87 23.5308549334505 26.41102142584723 26.27245172960236 23.78567191109991 88-89 23.57399745813027 26.436542066458006 26.255804478417815 23.733655996993914 90-91 23.540669251239468 26.108970716150637 26.35215178135747 23.998208251252425 92-93 23.432786306525514 26.361591591361822 26.41154149771791 23.79408060439475 94-95 23.457885479427578 26.135769888561168 26.317432391253487 24.088912240757768 96-97 23.500598439995944 26.200968808498935 26.271533512772454 24.026899238732668 98-99 23.46123911331126 26.167244679055067 26.38443774899238 23.9870784586413 100-101 23.371882326683235 26.029704330527316 26.38406998212956 24.214343360659893 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6075.0 1 3718.5 2 1486.0 3 1622.0 4 1488.5 5 1527.0 6 2554.5 7 5287.0 8 9290.5 9 11580.0 10 9830.0 11 7459.5 12 7265.5 13 7893.0 14 8532.5 15 9228.5 16 9889.5 17 10577.5 18 11465.0 19 12257.0 20 13631.0 21 15501.0 22 18044.5 23 22458.0 24 28816.5 25 36748.5 26 47482.0 27 59880.5 28 76412.5 29 96735.0 30 114457.5 31 133352.5 32 155219.0 33 172588.0 34 189144.0 35 211655.0 36 231567.0 37 253944.5 38 276204.5 39 291878.0 40 312909.0 41 331142.5 42 341271.0 43 349240.5 44 355755.5 45 362343.0 46 366568.5 47 363545.5 48 362131.5 49 365760.5 50 368883.0 51 366545.5 52 362061.5 53 366568.0 54 366414.0 55 363994.0 56 360336.5 57 339001.5 58 324110.0 59 320426.5 60 305010.0 61 278375.5 62 239134.0 63 191459.0 64 152515.5 65 121676.5 66 98203.5 67 79738.5 68 60431.5 69 44670.0 70 30775.5 71 19897.5 72 13501.0 73 9441.5 74 6921.5 75 5246.0 76 3802.0 77 2810.0 78 2128.0 79 1642.5 80 1331.0 81 1029.0 82 810.5 83 681.0 84 589.5 85 499.5 86 393.0 87 349.0 88 302.5 89 248.0 90 221.0 91 177.0 92 142.5 93 143.0 94 124.0 95 90.5 96 61.5 97 54.0 98 47.0 99 26.5 100 15.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01368368036691723 2 0.004626092328311384 3 0.004033457173441665 4 0.005643301922490752 5 0.002547446635857894 6 0.007253146671539837 7 0.0013091045212047512 8 0.0040246118726227145 9 0.010092488234423115 10-11 0.00762464930593578 12-13 0.009057588038605846 14-15 0.003498316473895129 16-17 0.0059440421503350865 18-19 0.003759252848054184 20-21 9.77405740494088E-4 22-23 0.0011410438056446818 24-25 0.006748964524859629 26-27 9.729830900846125E-4 28-29 7.297373175634592E-4 30-31 6.280163581455226E-4 32-33 0.0012825686187478982 34-35 0.001888471724846043 36-37 0.0014506293343079675 38-39 0.0019857700338545043 40-41 0.002631476993637929 42-43 0.002658012896094782 44-45 0.007270837273177739 46-47 0.014165749261550061 48-49 0.005258531336866382 50-51 0.005917506247878234 52-53 0.013643876513231953 54-55 0.008509179387830882 56-57 0.005678683125766556 58-59 0.0071514257121219 60-61 0.0012516100658815695 62-63 0.002105181594910343 64-65 0.002299778212927266 66-67 0.0024280350748020554 68-69 0.0020919136436819167 70-71 0.00229535556251779 72-73 0.0012781459683384225 74-75 0.0014992784888121981 76-77 0.008615322997658296 78-79 0.0070010555981997345 80-81 0.013391785439891848 82-83 0.015523502937259043 84-85 0.008624168298477246 86-87 0.005815785288460297 88-89 0.003308142506287682 90-91 0.0024899521805347128 92-93 9.86251041313039E-4 94-95 0.001176425008920486 96-97 0.0018663584727986655 98-99 0.006629552963803791 100-101 0.011454664560541573 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1305438E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.55852562634742 #Duplication Level Percentage of deduplicated Percentage of total 1 70.02671006707729 31.20286955052664 2 16.414715162238068 14.62831012411557 3 5.450805426939493 7.286395599015509 4 2.505834291520474 4.466251259763807 5 1.3226604483684072 2.946789974178992 6 0.8234962681124329 2.201626773953359 7 0.5824572585325144 1.8167405676401172 8 0.4220311911837183 1.5044070117982111 9 0.32110651784834837 1.287722970389953 >10 1.8741057784530293 15.476650966315638 >50 0.1484801600004129 4.555053951707811 >100 0.09761776780458113 8.128277516942013 >500 0.007528214334410723 2.2791131298446783 >1k 0.0023717263367385106 1.5983402387627872 >5k 1.9930312539413706E-5 0.07147149872646003 >10k+ 5.979093761824111E-5 0.5499788663182988 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26531 0.23467467602758954 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23672 0.20938596098620857 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11727 0.10372884270383864 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 8.845300818951022E-6 0.0 0.0 0.0 8 0.0 8.845300818951022E-6 0.0 0.0 0.0 9 0.0 8.845300818951022E-6 0.0 8.845300818951022E-6 0.0 10-11 0.0 8.845300818951022E-6 4.422650409475511E-6 1.7690601637902043E-5 0.0 12-13 0.0 8.845300818951022E-6 8.845300818951022E-6 3.0958552866328575E-5 0.0 14-15 0.0 1.3267951228426532E-5 8.845300818951022E-6 4.864915450423062E-5 0.0 16-17 0.0 1.7690601637902043E-5 8.845300818951022E-6 6.633975614213266E-5 8.845300818951022E-6 18-19 4.422650409475511E-6 1.7690601637902043E-5 8.845300818951022E-6 9.729830900846124E-5 8.845300818951022E-6 20-21 8.845300818951022E-6 1.7690601637902043E-5 8.845300818951022E-6 1.4594746351269186E-4 1.7690601637902043E-5 22-23 8.845300818951022E-6 1.7690601637902043E-5 8.845300818951022E-6 2.830496262064327E-4 2.6535902456853065E-5 24-25 8.845300818951022E-6 2.6535902456853065E-5 8.845300818951022E-6 5.218727483181103E-4 3.5381203275804086E-5 26-27 8.845300818951022E-6 3.98038536852796E-5 8.845300818951022E-6 9.022206835330042E-4 3.5381203275804086E-5 28-29 8.845300818951022E-6 4.422650409475511E-5 8.845300818951022E-6 0.001808864017475484 3.5381203275804086E-5 30-31 2.6535902456853065E-5 4.422650409475511E-5 8.845300818951022E-6 0.003931736214023729 3.5381203275804086E-5 32-33 2.6535902456853065E-5 4.422650409475511E-5 8.845300818951022E-6 0.008345541322680288 3.5381203275804086E-5 34-35 2.6535902456853065E-5 4.422650409475511E-5 8.845300818951022E-6 0.015735790156913867 3.5381203275804086E-5 36-37 2.6535902456853065E-5 6.633975614213266E-5 8.845300818951022E-6 0.027592915904717713 3.5381203275804086E-5 38-39 2.6535902456853065E-5 8.845300818951022E-5 8.845300818951022E-6 0.043275634256717876 3.5381203275804086E-5 40-41 2.6535902456853065E-5 8.845300818951022E-5 8.845300818951022E-6 0.058710684185787405 3.5381203275804086E-5 42-43 2.6535902456853065E-5 9.729830900846124E-5 8.845300818951022E-6 0.07463222565989924 3.5381203275804086E-5 44-45 2.6535902456853065E-5 9.729830900846124E-5 8.845300818951022E-6 0.09652434518680303 3.5381203275804086E-5 46-47 2.6535902456853065E-5 9.729830900846124E-5 8.845300818951022E-6 0.12334329726986253 3.5381203275804086E-5 48-49 3.5381203275804086E-5 9.729830900846124E-5 8.845300818951022E-6 0.15188708301261747 3.5381203275804086E-5 50-51 4.422650409475511E-5 1.1498891064636328E-4 8.845300818951022E-6 0.18062546537338936 3.98038536852796E-5 52-53 4.864915450423062E-5 1.1498891064636328E-4 8.845300818951022E-6 0.20859872921332195 4.422650409475511E-5 54-55 5.307180491370613E-5 1.1941156105583879E-4 8.845300818951022E-6 0.2423081706343443 4.422650409475511E-5 56-57 5.307180491370613E-5 1.238342114653143E-4 8.845300818951022E-6 0.28526095141117047 5.307180491370613E-5 58-59 5.307180491370613E-5 1.238342114653143E-4 8.845300818951022E-6 0.3336978186957462 6.191710573265715E-5 60-61 5.307180491370613E-5 1.3267951228426532E-4 8.845300818951022E-6 0.3849872954944337 7.076240655160817E-5 62-63 5.307180491370613E-5 1.3267951228426532E-4 8.845300818951022E-6 0.4382360064245189 7.076240655160817E-5 64-65 5.307180491370613E-5 1.3267951228426532E-4 8.845300818951022E-6 0.4956464313899205 7.518505696108368E-5 66-67 5.307180491370613E-5 1.4152481310321634E-4 8.845300818951022E-6 0.5646088192248722 7.96077073705592E-5 68-69 5.307180491370613E-5 1.4152481310321634E-4 8.845300818951022E-6 0.6448224297015295 7.96077073705592E-5 70-71 5.749445532318164E-5 1.4152481310321634E-4 8.845300818951022E-6 0.7350135395019636 8.845300818951022E-5 72-73 6.191710573265715E-5 1.4152481310321634E-4 8.845300818951022E-6 0.8294327030938562 8.845300818951022E-5 74-75 6.191710573265715E-5 1.4594746351269186E-4 8.845300818951022E-6 0.929079439469749 8.845300818951022E-5 76-77 6.191710573265715E-5 1.5037011392216737E-4 8.845300818951022E-6 1.0412820803581426 8.845300818951022E-5 78-79 6.191710573265715E-5 1.680607155600694E-4 8.845300818951022E-6 1.172409242348682 8.845300818951022E-5 80-81 6.191710573265715E-5 1.680607155600694E-4 8.845300818951022E-6 1.3190952884797564 8.845300818951022E-5 82-83 6.191710573265715E-5 1.7248336596954492E-4 8.845300818951022E-6 1.4781912916598188 8.845300818951022E-5 84-85 6.191710573265715E-5 1.7690601637902043E-4 8.845300818951022E-6 1.642789071949269 8.845300818951022E-5 86-87 6.191710573265715E-5 1.7690601637902043E-4 8.845300818951022E-6 1.8237241228513217 8.845300818951022E-5 88-89 6.191710573265715E-5 1.8575131719797145E-4 8.845300818951022E-6 2.028196519232603 8.845300818951022E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 19145 0.0 54.78772 1 GTATCAA 33280 0.0 42.52328 1 GTGGTAT 7620 0.0 36.595078 1 ATCAACG 41215 0.0 33.976532 3 TCAACGC 41205 0.0 33.882824 4 CAACGCA 42460 0.0 32.98101 5 TATCAAC 43600 0.0 32.171432 2 AACGCAG 44865 0.0 31.245098 6 TGGTATC 9260 0.0 28.674395 2 CGCAGAG 51910 0.0 26.58476 8 ACGCAGA 52380 0.0 26.55481 7 GCAGAGT 57265 0.0 23.609308 9 GTACATG 50050 0.0 22.684717 1 TACATGG 50110 0.0 21.906298 2 GAGTACT 27340 0.0 21.787714 12-13 CAGAGTA 55135 0.0 21.112984 10-11 ACATGGG 53275 0.0 20.052702 3 AGAGTAC 52860 0.0 19.743547 10-11 GTACTTT 31190 0.0 19.096783 14-15 AGTACTT 28525 0.0 18.31807 12-13 >>END_MODULE