FastQCFastQC Report
Wed 25 May 2016
SRR2049395_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049395_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11305438
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261160.23100387618772486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233810.2068119784478938No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137750.12184401878105032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA184000.053.1022831
GTATCAA314150.042.6901661
ATCAACG402350.032.853583
TCAACGC402200.032.7830244
CAACGCA417650.031.6042695
TATCAAC423300.031.4639242
GTGGTAT72700.031.1157631
AACGCAG438350.030.2089546
ACGCAGA522150.025.1965317
CGCAGAG520400.025.1899938
TGGTATC91700.023.9307212
GCAGAGT577350.022.4751999
GTACATG468950.021.595581
TACATGG476100.020.5219782
GAGTACT287500.020.2459212-13
AGAGTAC535250.019.899310-11
CAGAGTA561350.019.74404310-11
ACATGGG510450.018.7683563
AGTACTT298400.018.62297412-13
GTACTTT321750.018.13512414-15
Produced by FastQC (version 0.11.5)