##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049395_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11305438 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.216885449285556 33.0 31.0 34.0 31.0 34.0 2 32.40558870872584 34.0 31.0 34.0 31.0 34.0 3 32.477835622113886 34.0 31.0 34.0 31.0 34.0 4 36.09167252078159 37.0 35.0 37.0 35.0 37.0 5 35.986908777881936 37.0 35.0 37.0 35.0 37.0 6 36.014726806692494 37.0 35.0 37.0 35.0 37.0 7 35.95163274523287 37.0 35.0 37.0 35.0 37.0 8 35.94417845642071 37.0 35.0 37.0 35.0 37.0 9 37.59802919621513 39.0 37.0 39.0 35.0 39.0 10-11 37.583024647076925 39.0 37.0 39.0 35.0 39.0 12-13 37.567135258271286 39.0 37.0 39.0 35.0 39.0 14-15 38.84177592234816 40.0 38.0 41.0 35.0 41.0 16-17 38.80758582728064 40.0 38.0 41.0 35.0 41.0 18-19 38.80521152740832 40.0 38.0 41.0 35.0 41.0 20-21 38.774043871630624 40.0 38.0 41.0 35.0 41.0 22-23 38.69962649832762 40.0 38.0 41.0 34.5 41.0 24-25 38.59628592010323 40.0 38.0 41.0 34.0 41.0 26-27 38.43519282490426 40.0 38.0 41.0 34.0 41.0 28-29 38.261189084403455 40.0 38.0 41.0 34.0 41.0 30-31 38.10458037981368 40.0 38.0 41.0 34.0 41.0 32-33 37.90028435873073 40.0 37.0 41.0 33.0 41.0 34-35 37.74503800737309 40.0 37.0 41.0 33.0 41.0 36-37 37.585149332560135 39.5 37.0 41.0 33.0 41.0 38-39 37.80685967230991 40.0 37.0 41.0 33.0 41.0 40-41 37.75157211954105 40.0 37.0 41.0 33.0 41.0 42-43 37.578840112165494 40.0 37.0 41.0 33.0 41.0 44-45 37.368735249355225 40.0 37.0 41.0 32.5 41.0 46-47 37.09152276983873 39.5 36.0 41.0 31.5 41.0 48-49 36.85993271556573 39.0 36.0 41.0 31.0 41.0 50-51 36.636174025278805 39.0 35.5 40.5 31.0 41.0 52-53 36.43181431802996 39.0 35.0 40.0 31.0 41.0 54-55 36.30190873630902 39.0 35.0 40.0 30.5 41.0 56-57 36.17920645798951 39.0 35.0 40.0 30.5 41.0 58-59 35.90365711615949 38.0 35.0 40.0 30.0 41.0 60-61 35.61871609927895 38.0 35.0 40.0 29.5 41.0 62-63 35.29750019415435 37.0 34.0 40.0 29.0 41.0 64-65 35.0069016786435 37.0 34.0 40.0 29.0 41.0 66-67 34.63628322051741 36.0 34.0 39.0 28.0 41.0 68-69 34.29495217257394 36.0 34.0 39.0 28.0 41.0 70-71 33.949954305175964 35.0 33.5 38.5 28.0 40.0 72-73 33.582646510466915 35.0 33.0 37.5 27.0 40.0 74-75 33.212985335021955 35.0 33.0 37.0 26.0 39.5 76-77 31.233959621909385 33.5 30.0 35.5 24.5 38.0 78-79 32.58490551184306 35.0 32.5 36.0 26.0 39.0 80-81 32.628456986805816 35.0 33.0 36.0 26.5 37.5 82-83 32.397987853279105 35.0 33.0 35.5 26.0 37.0 84-85 32.18428768527146 35.0 33.0 35.0 26.0 37.0 86-87 31.961161566672608 35.0 33.0 35.0 26.0 36.0 88-89 31.731810346489894 35.0 32.5 35.0 25.0 36.0 90-91 31.4963255735868 35.0 32.0 35.0 25.0 36.0 92-93 31.283262001879095 34.5 32.0 35.0 24.5 35.5 94-95 31.055430846642118 34.0 32.0 35.0 24.0 35.0 96-97 30.837222803751608 34.0 32.0 35.0 23.0 35.0 98-99 30.5486963441841 34.0 32.0 35.0 20.0 35.0 100-101 29.907301291643897 34.0 30.5 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35006273597443993 1101 2 0.3784876763019227 1101 3 0.3524470468661107 1101 4 0.27914448203343767 1101 5 0.2928223278584312 1101 6 0.2733057145727926 1101 7 0.29811566708323767 1101 8 0.3025731200550936 1101 9 0.38803525489000634 1101 10-11 0.3641657119623076 1101 12-13 0.28746817664904256 1101 14-15 0.39937312151200643 1101 16-17 0.37458091570495355 1101 18-19 0.35540131643606543 1101 20-21 0.2888214525825603 1101 22-23 0.2795967809641766 1101 24-25 0.27071982582554455 1101 26-27 0.2548025214378171 1101 28-29 0.2197987645495445 1101 30-31 0.19117790156069248 1101 32-33 0.18542415305206816 1101 34-35 0.14006490450639575 1101 36-37 0.055508297920397354 1101 38-39 0.1806139828737514 1101 40-41 0.2003452989648764 1101 42-43 0.2457302234138723 1101 44-45 0.15248677179274495 1101 46-47 0.1989571324083741 1101 48-49 0.1133579655748278 1101 50-51 0.17478851499806325 1101 52-53 0.09821330121145166 1101 54-55 0.0846776147051429 1101 56-57 0.10259785758271533 1101 58-59 8.073216509529857E-4 1101 60-61 0.03108212205157912 1101 62-63 -0.029977558079245625 1101 64-65 -0.09342463811584878 1101 66-67 -0.13208400221623862 1101 68-69 -0.15087151064701487 1101 70-71 -0.21142826645805002 1101 72-73 -0.21917194459884115 1101 74-75 -0.25790802697709836 1101 76-77 -0.3202593382838934 1101 78-79 -0.29600507593431047 1101 80-81 -0.29051881603422913 1101 82-83 -0.300424673957842 1101 84-85 -0.31291521201717387 1101 86-87 -0.34468937991385573 1101 88-89 -0.4589996105069041 1101 90-91 -0.5088624514541031 1101 92-93 -0.5404164564318812 1101 94-95 -0.5411548183071098 1101 96-97 -0.6486925249033177 1101 98-99 -0.8148021588754446 1101 100-101 -0.9041505608130613 1102 1 0.34365927525582407 1102 2 0.3297629855825477 1102 3 0.29806133676592594 1102 4 0.2630094326068786 1102 5 0.26230807037198645 1102 6 0.23971715719543596 1102 7 0.2617867635775326 1102 8 0.2620938685272378 1102 9 0.35037851662423947 1102 10-11 0.3065749107435849 1102 12-13 0.2693674623202753 1102 14-15 0.3018140009964725 1102 16-17 0.3364125795115456 1102 18-19 0.3233471292165149 1102 20-21 0.2698985787388466 1102 22-23 0.2083954132279615 1102 24-25 0.24458114774730433 1102 26-27 0.23064816793935705 1102 28-29 0.22263377273519325 1102 30-31 0.1709456024939513 1102 32-33 0.17044767392867755 1102 34-35 0.1299349185699512 1102 36-37 0.0785939461229006 1102 38-39 0.1979982946883041 1102 40-41 0.21148806764789185 1102 42-43 0.25965867418133115 1102 44-45 0.20077506812827295 1102 46-47 0.20015179960566343 1102 48-49 0.011118735678486757 1102 50-51 0.06735639567747143 1102 52-53 0.10497401507382875 1102 54-55 0.09912599469411987 1102 56-57 0.04653938505222044 1102 58-59 0.014483328162029352 1102 60-61 0.09953458643217772 1102 62-63 0.02896752824465665 1102 64-65 0.019529684687938698 1102 66-67 -0.051122993720575494 1102 68-69 -0.07884225840505366 1102 70-71 -0.14816530250080007 1102 72-73 -0.18924736116555607 1102 74-75 -0.19380551873718588 1102 76-77 -0.18421444112271956 1102 78-79 -0.09054261973008693 1102 80-81 -0.0762378849659413 1102 82-83 0.008215882393457719 1102 84-85 -0.10621315611205517 1102 86-87 -0.1374409484766126 1102 88-89 -0.2282644224379311 1102 90-91 -0.28115096629950287 1102 92-93 -0.29925692752560096 1102 94-95 -0.3733979100506737 1102 96-97 -0.37791081634173906 1102 98-99 -0.4835786883981221 1102 100-101 -0.5614544790710028 1103 1 0.28875501534420067 1103 2 0.27380515114971615 1103 3 0.2552238033437817 1103 4 0.20688602961253366 1103 5 0.21094775118086773 1103 6 0.21360368057974455 1103 7 0.21948652160222792 1103 8 0.24824018503477419 1103 9 0.2714773504197794 1103 10-11 0.21860247471837368 1103 12-13 0.2228954301802446 1103 14-15 0.30306786668010943 1103 16-17 0.3314618320010112 1103 18-19 0.2810311773720997 1103 20-21 0.19087878020418714 1103 22-23 0.1769638171771959 1103 24-25 0.18929841999423758 1103 26-27 0.18485618903807932 1103 28-29 0.1964655436288396 1103 30-31 0.15410639025841988 1103 32-33 0.16799817968822595 1103 34-35 0.09818491340680424 1103 36-37 0.16096037220657422 1103 38-39 0.21226375697808209 1103 40-41 0.1818878741480887 1103 42-43 0.22797016259745106 1103 44-45 0.1966868782911746 1103 46-47 0.2649677222502973 1103 48-49 0.18428159566054347 1103 50-51 0.1870070750190891 1103 52-53 0.14567980588937957 1103 54-55 0.14103845197058718 1103 56-57 0.08972311572303937 1103 58-59 0.0593172448335082 1103 60-61 0.01468865027983668 1103 62-63 0.04551601508104852 1103 64-65 0.06695075226443947 1103 66-67 -0.010367551674178799 1103 68-69 -0.02453945984385797 1103 70-71 -0.05982474811955285 1103 72-73 -0.053392036916093844 1103 74-75 -0.03345087791731771 1103 76-77 -0.09494708954907694 1103 78-79 -0.009857203787127844 1103 80-81 -0.037960873949920426 1103 82-83 0.043004207957331175 1103 84-85 -0.00466282949606267 1103 86-87 -0.03502679177287504 1103 88-89 -0.09959683514663453 1103 90-91 -0.06740696956183356 1103 92-93 -0.15623817755315983 1103 94-95 -0.1094952134682714 1103 96-97 -0.15933420551356647 1103 98-99 -0.17706256574851764 1103 100-101 -0.23795656416744393 1104 1 0.32194485550458296 1104 2 0.3064875787868857 1104 3 0.302528877282235 1104 4 0.24380086580623583 1104 5 0.2556870629575627 1104 6 0.27802894461670036 1104 7 0.2273938386516221 1104 8 0.2706479475421162 1104 9 0.34442847504791985 1104 10-11 0.31173877564850727 1104 12-13 0.2592144546945292 1104 14-15 0.34859301073930027 1104 16-17 0.3746084784340411 1104 18-19 0.3161999992607605 1104 20-21 0.3079214496409506 1104 22-23 0.2844881911774735 1104 24-25 0.28692565316624297 1104 26-27 0.3066915687173477 1104 28-29 0.3185974738914936 1104 30-31 0.29885013452049947 1104 32-33 0.3412854101624774 1104 34-35 0.2749033909533054 1104 36-37 0.31219373290469576 1104 38-39 0.32177450397851004 1104 40-41 0.3571277214902011 1104 42-43 0.37406820653207973 1104 44-45 0.3753347511286762 1104 46-47 0.37841726551069854 1104 48-49 0.32683522292551714 1104 50-51 0.3558638359054527 1104 52-53 0.323107219157869 1104 54-55 0.34246238676303165 1104 56-57 0.3174314269338012 1104 58-59 0.4039947683105396 1104 60-61 0.3543897978106756 1104 62-63 0.36579866278450623 1104 64-65 0.31295391914541426 1104 66-67 0.3166390806997228 1104 68-69 0.27743152245549396 1104 70-71 0.3283548975522095 1104 72-73 0.26159997481591546 1104 74-75 0.2759409527433405 1104 76-77 0.20585436348614294 1104 78-79 0.3131181446242124 1104 80-81 0.34001498991437273 1104 82-83 0.3384412261893246 1104 84-85 0.2543788940401228 1104 86-87 0.20309763232859623 1104 88-89 0.1863742301536213 1104 90-91 0.21360473452289241 1104 92-93 0.17791071259213354 1104 94-95 0.17538180692849537 1104 96-97 0.048892193540318374 1104 98-99 0.07832694699104792 1104 100-101 0.10097199614900276 1105 1 0.2828897008027482 1105 2 0.27073499886441965 1105 3 0.25023173039245705 1105 4 0.22195384988489053 1105 5 0.19142510135358037 1105 6 0.18742012813123665 1105 7 0.18660590749122008 1105 8 0.2007938753406222 1105 9 0.26627879344047045 1105 10-11 0.20550395118431908 1105 12-13 0.22463508802565002 1105 14-15 0.2594202143783093 1105 16-17 0.25674211956005877 1105 18-19 0.2721441603910506 1105 20-21 0.23306127053101022 1105 22-23 0.16453264196644568 1105 24-25 0.207076685886868 1105 26-27 0.2600991375197239 1105 28-29 0.2060295394546543 1105 30-31 0.22366001900621058 1105 32-33 0.2372439104096955 1105 34-35 0.25806824498940983 1105 36-37 0.1870574245892982 1105 38-39 0.3172410928536351 1105 40-41 0.3149586338398933 1105 42-43 0.37029442296894644 1105 44-45 0.37743985458303086 1105 46-47 0.4226178020379834 1105 48-49 0.38290226942371675 1105 50-51 0.3760250971132848 1105 52-53 0.3793067935888885 1105 54-55 0.34950894592538617 1105 56-57 0.3845680476819169 1105 58-59 0.3384816480303954 1105 60-61 0.3774070899760389 1105 62-63 0.38942526236721875 1105 64-65 0.40515777595830826 1105 66-67 0.3956523097009992 1105 68-69 0.3439098683648467 1105 70-71 0.3138588762606247 1105 72-73 0.36240281118887907 1105 74-75 0.35049807981355485 1105 76-77 0.37970522187416833 1105 78-79 0.40356862892155476 1105 80-81 0.3238807387189482 1105 82-83 0.38851501245125775 1105 84-85 0.3972618644034611 1105 86-87 0.39146305881671495 1105 88-89 0.3801170844167494 1105 90-91 0.3988810927707078 1105 92-93 0.3715769308802699 1105 94-95 0.3314189773072478 1105 96-97 0.3580204251829855 1105 98-99 0.3124928922038812 1105 100-101 0.4089917175245965 1106 1 0.2701889965908748 1106 2 0.23533268171369315 1106 3 0.21037047253167884 1106 4 0.18980119641680915 1106 5 0.1937062932970477 1106 6 0.2241245759448276 1106 7 0.2502563197763834 1106 8 0.22989882491290103 1106 9 0.32231302785197613 1106 10-11 0.2456807928440199 1106 12-13 0.21029347987639113 1106 14-15 0.2541671132922261 1106 16-17 0.316640752316232 1106 18-19 0.2983248132546876 1106 20-21 0.24587640880332629 1106 22-23 0.24137022196608626 1106 24-25 0.2775048281160437 1106 26-27 0.2573304695131853 1106 28-29 0.31737354888185365 1106 30-31 0.3171656292539424 1106 32-33 0.3786557702247819 1106 34-35 0.3874995409578119 1106 36-37 0.3182482892172587 1106 38-39 0.40630356938063983 1106 40-41 0.3713590432530651 1106 42-43 0.4114585920846565 1106 44-45 0.4098388363964105 1106 46-47 0.45571317875317874 1106 48-49 0.4851636335923004 1106 50-51 0.5435368995180383 1106 52-53 0.5363639018517006 1106 54-55 0.5271176846093795 1106 56-57 0.4881772329234835 1106 58-59 0.5762813143788321 1106 60-61 0.5962291603477397 1106 62-63 0.5753794396982883 1106 64-65 0.511207047813194 1106 66-67 0.5579314610589847 1106 68-69 0.5443469483731818 1106 70-71 0.5784179234846718 1106 72-73 0.5660799926270528 1106 74-75 0.5426964192733053 1106 76-77 0.49153971190747825 1106 78-79 0.4966821868042288 1106 80-81 0.5755189915889432 1106 82-83 0.5886412290334491 1106 84-85 0.5815010110071981 1106 86-87 0.593051777228883 1106 88-89 0.5808629880021492 1106 90-91 0.5486932646458875 1106 92-93 0.5310851534603565 1106 94-95 0.5872537888561205 1106 96-97 0.6296062995889642 1106 98-99 0.6314406342161369 1106 100-101 0.6708615440770416 1107 1 0.2076222328328612 1107 2 0.20714041787781667 1107 3 0.19141491137883548 1107 4 0.1704050644271078 1107 5 0.15727536557592003 1107 6 0.16368140739410109 1107 7 0.1822503593865008 1107 8 0.187494985176059 1107 9 0.20113308449936085 1107 10-11 0.1790063760917775 1107 12-13 0.15322665763393672 1107 14-15 0.16816118447676587 1107 16-17 0.2005934466550059 1107 18-19 0.153496757514354 1107 20-21 0.17501023627637124 1107 22-23 0.15309202905640973 1107 24-25 0.18078175087280357 1107 26-27 0.2028576475563284 1107 28-29 0.2727535972911852 1107 30-31 0.25223358243481186 1107 32-33 0.22110955495754325 1107 34-35 0.1787357549548929 1107 36-37 0.20143291778364159 1107 38-39 0.23118532675560033 1107 40-41 0.21025618454788741 1107 42-43 0.2140798073698349 1107 44-45 0.15936608117640816 1107 46-47 0.1996571147776578 1107 48-49 0.12325252348583149 1107 50-51 0.1841715669424886 1107 52-53 0.1476300146742915 1107 54-55 0.17314485081211473 1107 56-57 0.25266332690187454 1107 58-59 0.27682830537223424 1107 60-61 0.24013127820214208 1107 62-63 0.28007301351390623 1107 64-65 0.32790466100211546 1107 66-67 0.3364120209022019 1107 68-69 0.33603930513832125 1107 70-71 0.3251912686440619 1107 72-73 0.34980637370306056 1107 74-75 0.3409313372838625 1107 76-77 0.32812440347919036 1107 78-79 0.40814386370745837 1107 80-81 0.4602593601342164 1107 82-83 0.499409273277827 1107 84-85 0.47941957483022435 1107 86-87 0.417683414943955 1107 88-89 0.40117165668813826 1107 90-91 0.4799099455569227 1107 92-93 0.49300944947630754 1107 94-95 0.5690852381293006 1107 96-97 0.5392345609936058 1107 98-99 0.5604972119936313 1107 100-101 0.7240463089875249 1108 1 0.13378867500367875 1108 2 0.12309558427979539 1108 3 0.09589842207967081 1108 4 0.08961243699982191 1108 5 0.08709900498222112 1108 6 0.11451565675007203 1108 7 0.11788885288695639 1108 8 0.14207552362864106 1108 9 0.1899244997999503 1108 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-0.6024923155116362 2316 98-99 -0.674924923559761 2316 100-101 -0.9555096382444894 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 28.0 4 70.0 5 159.0 6 330.0 7 909.0 8 2586.0 9 4932.0 10 7965.0 11 11312.0 12 15407.0 13 19917.0 14 26120.0 15 36152.0 16 50375.0 17 65421.0 18 53776.0 19 35374.0 20 30095.0 21 30087.0 22 32081.0 23 35130.0 24 42112.0 25 53000.0 26 68352.0 27 89018.0 28 116748.0 29 152715.0 30 201292.0 31 267776.0 32 362542.0 33 508634.0 34 749718.0 35 1165644.0 36 1846193.0 37 2629490.0 38 2204826.0 39 389030.0 40 114.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.75953171462457 20.190603319363568 15.69397770890534 24.355887257106517 2 15.548167209205047 20.770184245449723 37.6412304754515 26.040418069893732 3 17.89087925481294 24.743186952291747 29.95515507054459 27.41077872235072 4 13.789184665643733 17.480310976752637 35.6666909323332 33.06381342527043 5 14.845046836143288 36.34153543011075 33.85145081563654 14.961966918109415 6 32.58971827355535 35.72042194306312 16.873627072515692 14.81623271086583 7 28.87230021517079 31.470819617957307 21.071364063913315 18.585516102958593 8 27.01986018053616 33.85834035945186 19.51121566651653 19.610583793495447 9 26.323956952359524 16.085140695248334 20.054402186277244 37.53650016611489 10-11 25.34633339218408 25.94091019576777 27.310503548912223 21.402252863135928 12-13 26.03718228342856 23.527429897010624 27.27576764385422 23.159620175706593 14-15 23.520553669236005 24.940952881009373 25.17670351225762 26.361789937497004 16-17 23.08722138850348 27.915910909422525 25.78809418971649 23.208773512357507 18-19 23.065653136090077 26.739674940300816 27.957399412724733 22.23727251088437 20-21 24.110110152787325 25.664895357859457 27.768520638124052 22.456473851229166 22-23 23.855599402694526 25.731886725662463 27.62462188550324 22.787891986139766 24-25 23.505916415889747 25.870970215782034 27.637410074233333 22.98570329409489 26-27 23.32279792652154 25.964497482148495 27.735394999468575 22.977309591861385 28-29 23.377427098797245 25.725562088030777 27.930966887126647 22.966043926045327 30-31 23.41342452595577 25.84455848829494 27.688569622718123 23.053447363031175 32-33 23.309944356343735 25.867510900196226 27.82585746190515 22.996687281554884 34-35 23.678353976913407 25.784297256230115 27.52451837027841 23.012830396578067 36-37 23.686057536380574 25.712439991173742 27.468789085652638 23.132713386793043 38-39 23.473034193674163 25.89360301144648 27.64141984366038 22.99194295121897 40-41 23.487115745205255 25.86729604109403 27.560579676916646 23.085008536784066 42-43 23.481655087570974 25.930925061875914 27.334909763900733 23.25251008665238 44-45 23.442910874648994 26.138190404938968 27.14387284330138 23.275025877110657 46-47 23.645205621280773 26.05495650094099 27.06844041536229 23.231397462415945 48-49 23.74982502532572 26.03741682759914 26.785191691528 23.427566455547144 50-51 23.549253363927907 26.24426556332317 26.699637120676474 23.506843952072458 52-53 23.573523973738265 26.356567890677855 26.67017596823973 23.39973216734415 54-55 23.65483189431617 26.40665451695475 26.63842987167971 23.300083717049375 56-57 23.406265653452905 26.178548275945335 26.83913891513738 23.576047155464376 58-59 23.56324607070039 25.9879201488109 26.827973417374928 23.62086036311378 60-61 23.63649479601564 26.097384803195368 26.729035131820456 23.53708526896854 62-63 23.460499518448618 26.185585868642974 27.016107203739754 23.337807409168654 64-65 23.66664358019669 26.123840070734822 26.75732484600396 23.45219150306453 66-67 23.424346016620554 26.028638459528423 26.845756111697 23.701259412154023 68-69 23.45258927476741 26.079627450574517 26.85880625168847 23.608977022969597 70-71 23.474596094889932 26.010530676629433 26.828347388049256 23.686525840431383 72-73 23.40101001550502 26.190423118356755 26.74433682892848 23.664230037209748 74-75 23.387300544221702 26.285077983114352 26.635766593910056 23.691854878753894 76-77 23.41618204109602 26.360551340707605 26.523626660302636 23.699639957893734 78-79 23.46207810629449 26.301476724164313 26.538426088339502 23.698019081201696 80-81 23.322630110891176 26.364627691145 26.532022363280717 23.78071983468311 82-83 23.32311463948632 26.43113164088317 26.64564814198919 23.600105577641315 84-85 23.281545821965572 26.30360243155373 26.37240163165025 24.042450114830448 86-87 23.440440793827303 26.36193211124721 26.356394678155276 23.841232416770215 88-89 23.381887614385942 26.443943944829385 26.376545446375406 23.797622994409274 90-91 23.356932131213593 26.146029199117315 26.47608656376662 24.02095210590247 92-93 23.294646908609405 26.323859419765544 26.47790044253071 23.903593229094337 94-95 23.256458737970995 26.21057386650004 26.414476750984615 24.11849064454434 96-97 23.308320523091297 26.29157888787197 26.32548744154014 24.074613147496592 98-99 23.18782379816237 26.23012729930114 26.474268034436214 24.107780868100274 100-101 23.007614698679284 26.191250891440514 26.49940349499964 24.30173091488056 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6031.0 1 3856.0 2 1677.0 3 1632.5 4 1409.0 5 1445.0 6 2692.5 7 6016.0 8 10101.5 9 12152.5 10 10354.0 11 7603.5 12 7135.5 13 7708.0 14 8551.0 15 9225.0 16 9834.5 17 10577.0 18 11226.5 19 12078.5 20 13417.0 21 15082.5 22 17915.0 23 22374.5 24 28372.5 25 36230.0 26 46896.0 27 59565.0 28 76542.0 29 97026.0 30 114663.5 31 133968.5 32 155733.5 33 173167.5 34 190421.5 35 212779.5 36 232886.0 37 255204.5 38 277684.5 39 294284.5 40 315224.5 41 333126.0 42 343169.0 43 352766.0 44 359856.0 45 365420.0 46 370446.0 47 368272.0 48 366095.5 49 369624.0 50 373058.0 51 370831.5 52 366522.0 53 369320.5 54 368019.5 55 364514.5 56 360767.5 57 339145.0 58 323778.0 59 320280.5 60 304571.0 61 276590.5 62 236180.0 63 188668.0 64 148120.0 65 116342.0 66 93132.5 67 75029.0 68 56035.0 69 40293.5 70 26719.0 71 16210.0 72 10364.5 73 7191.0 74 5303.0 75 3798.5 76 2533.0 77 1757.5 78 1234.0 79 840.0 80 566.0 81 381.0 82 266.0 83 168.5 84 101.0 85 81.5 86 63.5 87 44.0 88 34.5 89 26.0 90 14.0 91 11.0 92 12.0 93 6.5 94 3.0 95 1.5 96 0.5 97 1.5 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04705700035681944 2 0.0021847893022809025 3 3.0958552866328575E-4 4 2.3882312211167758E-4 5 0.002476684229306286 6 0.0019105849768934207 7 0.0 8 1.7690601637902043E-5 9 8.845300818951022E-5 10-11 1.7690601637902043E-5 12-13 0.0 14-15 5.307180491370613E-4 16-17 0.0 18-19 6.191710573265715E-5 20-21 4.422650409475511E-4 22-23 0.0 24-25 6.32439008554998E-4 26-27 0.007602536053888403 28-29 0.007890008330504312 30-31 0.0012162288626057654 32-33 0.007297373175634593 34-35 0.008447262282098226 36-37 0.004763194491005125 38-39 0.0027066620505990127 40-41 0.0021582533998240495 42-43 0.0013356404236616042 44-45 7.076240655160817E-4 46-47 6.943561142876552E-4 48-49 0.0020388418387682107 50-51 0.001455051984717443 52-53 0.002162676050233525 54-55 8.358809273908716E-4 56-57 2.2113252047377554E-4 58-59 7.385826183824103E-4 60-61 0.006072299012209876 62-63 0.0027420432538748166 64-65 0.0010172095941793674 66-67 0.005660992524128654 68-69 0.007465433891194662 70-71 0.0024457256764399576 72-73 5.926351548697185E-4 74-75 0.0034894711730761776 76-77 0.0020565324404061124 78-79 0.002476684229306286 80-81 0.0010614360982741226 82-83 1.238342114653143E-4 84-85 6.545522606023756E-4 86-87 0.004962213759431524 88-89 0.0025651372374957964 90-91 0.0011543117568731083 92-93 7.076240655160817E-5 94-95 4.953368458612572E-4 96-97 2.7420432538748167E-4 98-99 6.501296101929002E-4 100-101 4.422650409475511E-6 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1305438E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.02966814368868 #Duplication Level Percentage of deduplicated Percentage of total 1 68.4339678037184 29.446909243498787 2 16.951476162050483 14.588327875973636 3 5.889819927612376 7.603109907337352 4 2.655868151835751 4.571245008275366 5 1.4564998591749534 3.1336352795813776 6 0.9011020669254574 2.326447374203663 7 0.6344651596646352 1.9110577688371198 8 0.4701589351429376 1.6184626363192522 9 0.33687304467711926 1.3045981787109409 >10 1.9953106324445606 15.884233613034974 >50 0.16107632825559784 4.774028895873306 >100 0.10325555122055703 8.346162034038048 >500 0.007660366670068769 2.282456134233607 >1k 0.002362397398827224 1.5296811576897347 >5k 4.144528324205613E-5 0.11545581299789874 >10k+ 6.21679248630842E-5 0.5641890793947365 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26116 0.23100387618772486 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23381 0.2068119784478938 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13775 0.12184401878105032 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 3.5381203275804086E-5 0.0 0.0 0.0 3 0.0 3.5381203275804086E-5 0.0 0.0 0.0 4 0.0 3.5381203275804086E-5 0.0 0.0 0.0 5 0.0 3.5381203275804086E-5 0.0 0.0 0.0 6 0.0 3.5381203275804086E-5 0.0 0.0 0.0 7 0.0 3.5381203275804086E-5 0.0 0.0 0.0 8 0.0 3.5381203275804086E-5 0.0 8.845300818951022E-6 0.0 9 0.0 3.5381203275804086E-5 0.0 8.845300818951022E-6 0.0 10-11 0.0 3.5381203275804086E-5 0.0 2.2113252047377554E-5 0.0 12-13 4.422650409475511E-6 3.98038536852796E-5 0.0 4.864915450423062E-5 0.0 14-15 8.845300818951022E-6 6.191710573265715E-5 0.0 6.633975614213266E-5 0.0 16-17 8.845300818951022E-6 7.96077073705592E-5 0.0 8.40303577800347E-5 0.0 18-19 8.845300818951022E-6 7.96077073705592E-5 0.0 9.729830900846124E-5 0.0 20-21 8.845300818951022E-6 7.96077073705592E-5 0.0 1.5479276433164288E-4 1.7690601637902043E-5 22-23 8.845300818951022E-6 7.96077073705592E-5 0.0 2.830496262064327E-4 1.7690601637902043E-5 24-25 8.845300818951022E-6 7.96077073705592E-5 0.0 5.218727483181102E-4 1.7690601637902043E-5 26-27 8.845300818951022E-6 7.96077073705592E-5 0.0 9.022206835330042E-4 1.7690601637902043E-5 28-29 8.845300818951022E-6 7.96077073705592E-5 0.0 0.0017779054646091556 1.7690601637902043E-5 30-31 8.845300818951022E-6 7.96077073705592E-5 0.0 0.0038742417587005473 1.7690601637902043E-5 32-33 8.845300818951022E-6 8.845300818951022E-5 0.0 0.00830573746899501 2.6535902456853065E-5 34-35 8.845300818951022E-6 8.845300818951022E-5 0.0 0.01561637859585803 3.5381203275804086E-5 36-37 8.845300818951022E-6 1.1056626023688777E-4 0.0 0.02739831928670079 3.5381203275804086E-5 38-39 8.845300818951022E-6 1.238342114653143E-4 0.0 0.04303238848419672 4.422650409475511E-5 40-41 8.845300818951022E-6 1.238342114653143E-4 0.0 0.058414366608352544 4.422650409475511E-5 42-43 8.845300818951022E-6 1.238342114653143E-4 0.0 0.07436686663533071 4.422650409475511E-5 44-45 8.845300818951022E-6 1.282568618747898E-4 8.845300818951022E-6 0.09628552206469135 5.307180491370613E-5 46-47 8.845300818951022E-6 1.4152481310321634E-4 8.845300818951022E-6 0.12310889679816031 5.307180491370613E-5 48-49 1.7690601637902043E-5 1.5037011392216737E-4 1.7690601637902043E-5 0.15163941458968683 5.307180491370613E-5 50-51 1.7690601637902043E-5 1.5037011392216737E-4 1.7690601637902043E-5 0.18020973623489864 5.307180491370613E-5 52-53 1.7690601637902043E-5 1.5037011392216737E-4 1.7690601637902043E-5 0.2080282073104996 5.307180491370613E-5 54-55 1.7690601637902043E-5 1.636380651505939E-4 1.7690601637902043E-5 0.2414369085036776 5.749445532318164E-5 56-57 1.7690601637902043E-5 1.7690601637902043E-4 1.7690601637902043E-5 0.28408010375184045 6.633975614213266E-5 58-59 1.7690601637902043E-5 1.7690601637902043E-4 1.7690601637902043E-5 0.33229583851594247 7.076240655160817E-5 60-61 3.0958552866328575E-5 1.7690601637902043E-4 1.7690601637902043E-5 0.3831916994281867 7.076240655160817E-5 62-63 4.422650409475511E-5 1.8132866678849594E-4 1.7690601637902043E-5 0.436144092780837 7.076240655160817E-5 64-65 4.422650409475511E-5 1.9459661801692247E-4 2.6535902456853065E-5 0.49320955101429953 7.076240655160817E-5 66-67 4.422650409475511E-5 1.9459661801692247E-4 2.6535902456853065E-5 0.5617429417595321 7.076240655160817E-5 68-69 4.422650409475511E-5 1.9459661801692247E-4 2.6535902456853065E-5 0.6414656380407375 7.96077073705592E-5 70-71 4.422650409475511E-5 1.9459661801692247E-4 2.6535902456853065E-5 0.7307589498080481 8.845300818951022E-5 72-73 4.422650409475511E-5 2.1228721965482452E-4 2.6535902456853065E-5 0.824435108131149 8.845300818951022E-5 74-75 4.422650409475511E-5 2.2113252047377554E-4 2.6535902456853065E-5 0.9234228695960298 8.845300818951022E-5 76-77 4.422650409475511E-5 2.2113252047377554E-4 2.6535902456853065E-5 1.0352628531508465 8.845300818951022E-5 78-79 4.422650409475511E-5 2.2113252047377554E-4 2.6535902456853065E-5 1.1658283385393826 8.845300818951022E-5 80-81 4.422650409475511E-5 2.2113252047377554E-4 2.6535902456853065E-5 1.3119173268651776 8.845300818951022E-5 82-83 4.422650409475511E-5 2.2555517088325105E-4 2.6535902456853065E-5 1.4701155320121166 8.845300818951022E-5 84-85 4.422650409475511E-5 2.3440047170220207E-4 2.6535902456853065E-5 1.6339083899270421 8.845300818951022E-5 86-87 4.422650409475511E-5 2.3882312211167758E-4 2.6535902456853065E-5 1.814303877479139 8.845300818951022E-5 88-89 4.422650409475511E-5 2.3882312211167758E-4 2.6535902456853065E-5 2.018245555811283 8.845300818951022E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 18400 0.0 53.102283 1 GTATCAA 31415 0.0 42.690166 1 ATCAACG 40235 0.0 32.85358 3 TCAACGC 40220 0.0 32.783024 4 CAACGCA 41765 0.0 31.604269 5 TATCAAC 42330 0.0 31.463924 2 GTGGTAT 7270 0.0 31.115763 1 AACGCAG 43835 0.0 30.208954 6 ACGCAGA 52215 0.0 25.196531 7 CGCAGAG 52040 0.0 25.189993 8 TGGTATC 9170 0.0 23.930721 2 GCAGAGT 57735 0.0 22.475199 9 GTACATG 46895 0.0 21.59558 1 TACATGG 47610 0.0 20.521978 2 GAGTACT 28750 0.0 20.24592 12-13 AGAGTAC 53525 0.0 19.8993 10-11 CAGAGTA 56135 0.0 19.744043 10-11 ACATGGG 51045 0.0 18.768356 3 AGTACTT 29840 0.0 18.622974 12-13 GTACTTT 32175 0.0 18.135124 14-15 >>END_MODULE