FastQCFastQC Report
Wed 25 May 2016
SRR2049394_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049394_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8572395
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72900.039.4060971
GTATCAA118450.032.2964061
TCAACGC117050.030.982264
ATCAACG120850.030.0473353
CAACGCA122600.029.7295535
AACGCAG127850.028.5273866
TATCAAC143300.025.7365782
GTACATG289800.022.6364971
ACGCAGA159650.022.609197
TACATGG292650.021.3768062
CGCAGAG171400.020.5095088
ACATGGG301700.020.044043
CATGGGG250050.018.1477244
GTATATA47250.016.3337861
GCAGAGT211800.016.1718259
GTGGTAT47400.016.0810831
CAGAGTA195700.015.48000610-11
GAGTACA212050.015.4344851
TATACTG45650.014.8666425
GTATAGA32150.014.8181051
Produced by FastQC (version 0.11.5)