##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049394_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8572395 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.831636549645694 31.0 31.0 33.0 28.0 34.0 2 30.918051839655078 31.0 31.0 34.0 27.0 34.0 3 31.02264443017383 31.0 31.0 34.0 28.0 34.0 4 34.646423899038716 35.0 35.0 37.0 32.0 37.0 5 34.840298656326496 37.0 35.0 37.0 32.0 37.0 6 34.97279803368837 37.0 35.0 37.0 32.0 37.0 7 34.94520807778923 37.0 35.0 37.0 32.0 37.0 8 34.876173228135194 37.0 35.0 37.0 32.0 37.0 9 36.56849970165864 39.0 37.0 39.0 32.0 39.0 10-11 36.53814575739919 39.0 36.0 39.0 32.0 39.0 12-13 36.55758484064255 39.0 37.0 39.0 32.0 39.0 14-15 37.625814489416314 40.0 37.0 41.0 32.0 41.0 16-17 37.54868452748619 40.0 37.0 41.0 31.5 41.0 18-19 37.54385874659299 40.0 37.0 41.0 31.5 41.0 20-21 37.65629097819221 40.0 37.0 41.0 32.0 41.0 22-23 37.47134803050956 40.0 37.0 41.0 31.0 41.0 24-25 37.46819500268011 40.0 36.5 41.0 31.5 41.0 26-27 37.46092451409437 40.0 37.0 41.0 31.0 41.0 28-29 37.088766966524524 40.0 36.0 41.0 30.0 41.0 30-31 37.11102667340924 40.0 36.0 41.0 30.0 41.0 32-33 37.10469670377998 40.0 36.0 41.0 30.0 41.0 34-35 37.177108789317344 40.0 36.0 41.0 30.0 41.0 36-37 37.18640018337933 40.0 36.0 41.0 30.0 41.0 38-39 36.950905493738915 40.0 36.0 41.0 30.0 41.0 40-41 37.08517024705465 40.0 36.0 41.0 30.0 41.0 42-43 36.67317278310204 39.0 35.0 41.0 30.0 41.0 44-45 36.57318963953481 39.0 35.0 40.0 30.0 41.0 46-47 36.48019421643543 39.0 35.0 40.0 29.5 41.0 48-49 36.32282617634861 38.0 35.0 40.0 29.0 41.0 50-51 36.19236905205605 38.0 34.0 40.0 29.0 41.0 52-53 36.02570920961995 38.0 34.0 40.0 28.5 41.0 54-55 35.80509618373861 38.0 34.0 40.0 28.0 41.0 56-57 35.54371572938484 38.0 34.0 40.0 28.0 41.0 58-59 35.23920561290048 37.5 33.0 40.0 27.5 41.0 60-61 34.9418387160181 37.0 33.0 40.0 27.0 41.0 62-63 34.63817620396634 37.0 33.0 40.0 26.5 41.0 64-65 34.29902658475257 36.0 32.5 39.0 26.0 41.0 66-67 33.9769888111782 36.0 32.0 39.0 26.0 40.5 68-69 33.628860370993166 35.0 32.0 39.0 26.0 40.0 70-71 33.258553298115636 35.0 32.0 38.0 26.0 40.0 72-73 32.8667161277566 35.0 31.0 37.5 25.0 40.0 74-75 32.461334084581964 35.0 31.0 37.0 24.0 39.0 76-77 32.09565057373115 34.5 31.0 36.5 24.0 39.0 78-79 31.887503958928633 34.0 31.0 36.0 24.0 39.0 80-81 31.767824219485917 34.0 31.0 36.0 24.0 38.5 82-83 31.477563971328898 34.0 31.0 35.5 24.0 37.0 84-85 31.16915984389427 34.0 31.0 35.0 23.0 37.0 86-87 30.759541878319887 34.0 30.5 35.0 21.0 36.5 88-89 30.346619585308424 34.0 30.0 35.0 19.5 36.0 90-91 30.135995016561882 34.0 30.0 35.0 18.5 36.0 92-93 29.903904218132737 34.0 30.0 35.0 17.5 35.5 94-95 29.625441139844817 34.0 30.0 35.0 9.5 35.0 96-97 29.333229570032643 34.0 30.0 35.0 2.0 35.0 98-99 29.000301724313918 34.0 29.5 35.0 2.0 35.0 100-101 28.399220871180106 33.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2122792225158463 1101 2 0.23953923760065265 1101 3 0.18581295170669065 1101 4 0.2054882350888576 1101 5 0.24802195026538953 1101 6 0.08694755755473693 1101 7 0.20095799490235322 1101 8 0.31220936493335927 1101 9 0.29378486674794857 1101 10-11 0.3331370937443978 1101 12-13 0.2978943996967871 1101 14-15 0.4754218172983471 1101 16-17 0.41269950534087485 1101 18-19 0.40282366917956125 1101 20-21 0.49254080311194315 1101 22-23 0.4380241830455631 1101 24-25 0.05710369483919919 1101 26-27 0.32324155875844696 1101 28-29 0.7997191389540319 1101 30-31 0.36048152032363845 1101 32-33 0.7221695988369561 1101 34-35 0.5191512052450022 1101 36-37 0.4649069556035883 1101 38-39 0.4807400120341967 1101 40-41 0.3482071794061312 1101 42-43 0.44276699929250185 1101 44-45 0.2565312797226582 1101 46-47 0.14805314233080225 1101 48-49 0.038376415645537065 1101 50-51 0.09253451368429921 1101 52-53 0.020253875799824073 1101 54-55 -0.0038271040250918986 1101 56-57 -0.15592726634438492 1101 58-59 -0.23095095347944294 1101 60-61 -0.1630899439438025 1101 62-63 -0.2310792529739203 1101 64-65 -0.23772224105205453 1101 66-67 -0.30887264615282106 1101 68-69 -0.5073786641555813 1101 70-71 -0.47895159465698356 1101 72-73 -0.5430164918841598 1101 74-75 -0.4946854810218184 1101 76-77 -0.6346498300857206 1101 78-79 -0.5508240549087411 1101 80-81 -0.6496786908667254 1101 82-83 -0.8067886732124983 1101 84-85 -0.7406548092919927 1101 86-87 -0.7939537898036768 1101 88-89 -0.9476197762194865 1101 90-91 -1.0237130760844266 1101 92-93 -1.0686180199171709 1101 94-95 -1.151704262598681 1101 96-97 -1.2378558591854798 1101 98-99 -1.2885307902854564 1101 100-101 -1.5188709313326108 1102 1 0.16732001043937927 1102 2 0.06769375139842282 1102 3 0.035793922018978463 1102 4 0.10696369685293661 1102 5 0.25650005762837935 1102 6 8.135668920772332E-5 1102 7 0.04070572759150082 1102 8 0.16376915931994063 1102 9 0.23537576540923055 1102 10-11 0.3474507391064776 1102 12-13 0.35855069223515557 1102 14-15 0.5531230561760125 1102 16-17 0.5902160229365307 1102 18-19 0.5637768237282899 1102 20-21 0.4380157507474607 1102 22-23 -0.1667767508819864 1102 24-25 0.42149027848735443 1102 26-27 0.5858873018796302 1102 28-29 0.6625852843710334 1102 30-31 0.5202103049327818 1102 32-33 0.8190659487198317 1102 34-35 0.2027304376932122 1102 36-37 0.39952385520715694 1102 38-39 0.464483035623239 1102 40-41 0.3590223778020132 1102 42-43 0.3096980665474831 1102 44-45 0.0206402881683303 1102 46-47 -0.11315318056731627 1102 48-49 -0.22120229741992148 1102 50-51 -0.09180528024059242 1102 52-53 -0.047104125531276964 1102 54-55 -0.09357482832988495 1102 56-57 -0.21769394353621152 1102 58-59 -0.28887256894090996 1102 60-61 -0.2887561185607126 1102 62-63 -0.36091005336610493 1102 64-65 -0.40949353131519217 1102 66-67 -0.4509105147003538 1102 68-69 -0.49881181958970444 1102 70-71 -0.616431955007549 1102 72-73 -0.6497009959834656 1102 74-75 -0.6380116166671286 1102 76-77 -0.6476414333792491 1102 78-79 -0.6729186793810911 1102 80-81 -0.6371387331339271 1102 82-83 -0.7044863269406001 1102 84-85 -0.7508947935249353 1102 86-87 -0.7743034251709346 1102 88-89 -0.8617922458369698 1102 90-91 -0.8642430745467351 1102 92-93 -0.9455139416390601 1102 94-95 -0.9895275681613569 1102 96-97 -1.114274596605803 1102 98-99 -1.1936531356104823 1102 100-101 -1.3605101406388869 1103 1 0.11010282444484432 1103 2 0.03287909722337545 1103 3 -0.011201071830569731 1103 4 0.0193273538480625 1103 5 -0.11560185565964076 1103 6 -0.12518803107844434 1103 7 -0.08668461782160364 1103 8 0.09476491056891945 1103 9 0.1961107231182524 1103 10-11 0.2033022136257614 1103 12-13 0.22700288703582316 1103 14-15 0.309762614027008 1103 16-17 0.3371848188320854 1103 18-19 0.4095144781347244 1103 20-21 0.3828569925133891 1103 22-23 -0.05441903243155366 1103 24-25 0.32046112441979346 1103 26-27 0.491074419868859 1103 28-29 0.4035055400548586 1103 30-31 0.5050456030272201 1103 32-33 0.6240542322702112 1103 34-35 0.5688169469944242 1103 36-37 0.3626091265567766 1103 38-39 0.5909750885687473 1103 40-41 0.350180363256257 1103 42-43 -0.04534016059099599 1103 44-45 0.09008891950039555 1103 46-47 0.0019866307185552046 1103 48-49 -0.052073886089139876 1103 50-51 -0.04213721498256007 1103 52-53 -0.1759077676791847 1103 54-55 -0.12227941213445348 1103 56-57 -0.1942793811650816 1103 58-59 -0.19704468146667153 1103 60-61 -0.2742311987182475 1103 62-63 -0.2642078340422316 1103 64-65 -0.3568012771256548 1103 66-67 -0.47713268428742595 1103 68-69 -0.5034685956315457 1103 70-71 -0.5290467654474966 1103 72-73 -0.6214015490969587 1103 74-75 -0.47257909921293617 1103 76-77 -0.5404503428026857 1103 78-79 -0.486080681854947 1103 80-81 -0.637920732661577 1103 82-83 -0.6844871455198565 1103 84-85 -0.6959517454502375 1103 86-87 -0.6488715263507494 1103 88-89 -0.6466966361218844 1103 90-91 -0.7412000685459823 1103 92-93 -0.7981235261596744 1103 94-95 -0.771299260767929 1103 96-97 -0.7852859709268891 1103 98-99 -0.9950280069704966 1103 100-101 -0.9599940511987661 1104 1 0.21319662506677162 1104 2 0.2662341751752102 1104 3 0.09480059664377904 1104 4 0.22897091577215178 1104 5 0.07699802734997263 1104 6 -0.018632028600428896 1104 7 0.08058954590831746 1104 8 0.14913394170957872 1104 9 0.3015081734079601 1104 10-11 0.23977350842282874 1104 12-13 0.283196116644433 1104 14-15 0.46542882382151163 1104 16-17 0.5575263328092888 1104 18-19 0.39168808566734015 1104 20-21 0.4165317091210383 1104 22-23 0.5774320826901942 1104 24-25 0.7075478059404645 1104 26-27 0.612671113456706 1104 28-29 0.5347330463821862 1104 30-31 0.7021873773971379 1104 32-33 0.7363512459399288 1104 34-35 0.587709619483114 1104 36-37 0.3643532412410124 1104 38-39 0.4480180045220905 1104 40-41 0.3460068821752955 1104 42-43 0.4337004558056208 1104 44-45 0.22451795683339526 1104 46-47 0.0660071654534562 1104 48-49 0.009801685396794824 1104 50-51 -0.017557836316044018 1104 52-53 -0.018292524593071846 1104 54-55 0.03747436081464173 1104 56-57 0.05489949717621556 1104 58-59 -0.002646118709982659 1104 60-61 -0.03651641068621814 1104 62-63 -0.07384249550683109 1104 64-65 -0.03134503134127442 1104 66-67 -0.12898099042777034 1104 68-69 -0.12264554463543931 1104 70-71 -0.18470787602041838 1104 72-73 -0.21194396064569787 1104 74-75 -0.1238043844957275 1104 76-77 -0.2230959686564944 1104 78-79 -0.2846481103882823 1104 80-81 -0.3011352941570138 1104 82-83 -0.3217212522072366 1104 84-85 -0.3764169848594783 1104 86-87 -0.3456255224444966 1104 88-89 -0.29073646242181894 1104 90-91 -0.22250464887258659 1104 92-93 -0.2213656334013443 1104 94-95 -0.31849443337216954 1104 96-97 -0.3439837889304016 1104 98-99 -0.35381552378662207 1104 100-101 -0.3751563231278823 1105 1 0.198124951858059 1105 2 0.17439442339354372 1105 3 0.13641908155555527 1105 4 0.16866970438928064 1105 5 0.08197871495232079 1105 6 0.06221527917938374 1105 7 0.14684028334560395 1105 8 -0.10806434739953374 1105 9 -0.09939791552973531 1105 10-11 0.18719784033941522 1105 12-13 0.24115365164770708 1105 14-15 0.3326751915046984 1105 16-17 0.030755617108262356 1105 18-19 0.1752757214563445 1105 20-21 0.13628580228105847 1105 22-23 0.45739230788595364 1105 24-25 0.15528017946459727 1105 26-27 -0.09489347616992916 1105 28-29 0.4541251687005996 1105 30-31 0.36716097728923813 1105 32-33 0.348869685195595 1105 34-35 -0.30865027826376235 1105 36-37 -0.36560943426584913 1105 38-39 -0.16230838587133434 1105 40-41 -0.13062951351700036 1105 42-43 0.1941511655520145 1105 44-45 -0.016413988973617677 1105 46-47 -0.22666848315900268 1105 48-49 -0.2762160334730197 1105 50-51 -0.34850474133524045 1105 52-53 -0.10258389434552129 1105 54-55 -0.3077455118548258 1105 56-57 -0.5121635442764116 1105 58-59 -0.20438573393193593 1105 60-61 -0.17760225520126482 1105 62-63 -0.25021264131951426 1105 64-65 -0.42337448002238887 1105 66-67 -0.39304993224629925 1105 68-69 -0.36029274417055035 1105 70-71 -0.12402306010987019 1105 72-73 -0.2133735023630834 1105 74-75 -0.0641664993333464 1105 76-77 -0.15972922708298398 1105 78-79 -0.09068035788162376 1105 80-81 -0.14853090593672036 1105 82-83 -0.2405065796828474 1105 84-85 -0.3551420013770432 1105 86-87 -0.14265146680292418 1105 88-89 -0.24722314256711186 1105 90-91 -0.06885740909135407 1105 92-93 -0.062491488935652484 1105 94-95 -0.08672891454167697 1105 96-97 -0.20485211998755304 1105 98-99 -0.1938284993803201 1105 100-101 -0.25894828701429873 1106 1 0.32387958655955984 1106 2 0.31969403465750545 1106 3 0.24699311290239834 1106 4 0.337906029455155 1106 5 0.3547770450358527 1106 6 0.11408771999663259 1106 7 0.1547140732719896 1106 8 0.22123834181626734 1106 9 0.29980373816900396 1106 10-11 0.014004592225745682 1106 12-13 0.2256844445430346 1106 14-15 0.4760772705717571 1106 16-17 0.4141067571761283 1106 18-19 0.39325656698675004 1106 20-21 0.36217436140790227 1106 22-23 0.5211092013231067 1106 24-25 0.47340552620165965 1106 26-27 0.5278529998872301 1106 28-29 0.554158800153175 1106 30-31 0.5792411789065497 1106 32-33 0.6349558968383775 1106 34-35 0.5301336919678548 1106 36-37 0.2653227077765834 1106 38-39 0.4666496372295299 1106 40-41 0.28887668219199014 1106 42-43 -0.671639961485802 1106 44-45 0.07827098451046055 1106 46-47 0.11780817180319048 1106 48-49 0.0751212100059675 1106 50-51 0.08693548389705086 1106 52-53 0.10281916489083898 1106 54-55 0.032330561373548505 1106 56-57 -0.09880519084369865 1106 58-59 -0.03970778126105046 1106 60-61 0.028926624435683834 1106 62-63 -0.10212166491812269 1106 64-65 0.11615826056009126 1106 66-67 0.04475842415131126 1106 68-69 -0.04652500082325872 1106 70-71 0.06473558618849751 1106 72-73 0.07360841010692099 1106 74-75 0.1053733420661942 1106 76-77 -0.029100540354328075 1106 78-79 -0.06209658266143947 1106 80-81 0.005375784857843513 1106 82-83 0.02949078480020617 1106 84-85 0.047309314139287295 1106 86-87 0.15551669705007498 1106 88-89 0.05866893618711444 1106 90-91 0.09626963981594017 1106 92-93 0.1767061697229515 1106 94-95 0.3063288123238763 1106 96-97 0.23272217427366115 1106 98-99 0.20490769458092473 1106 100-101 0.19184935688020133 1107 1 0.10237418411003318 1107 2 -0.10382811698472239 1107 3 0.0620307109654199 1107 4 0.09442366628215382 1107 5 -0.031458827253118216 1107 6 -0.09592538929497607 1107 7 -0.026630585604259238 1107 8 -0.531282476297541 1107 9 -0.21707743419958803 1107 10-11 -0.4716358068280897 1107 12-13 -0.278005406293687 1107 14-15 -0.08062600086672944 1107 16-17 0.13571018121771772 1107 18-19 0.0017895583093689993 1107 20-21 -0.4128326002397742 1107 22-23 0.07636482346971007 1107 24-25 0.2702427632447666 1107 26-27 -0.161035770265876 1107 28-29 -0.4459331573046086 1107 30-31 -0.18832921164865724 1107 32-33 -0.5452482850919438 1107 34-35 -0.42657559948255397 1107 36-37 -0.9903423128462023 1107 38-39 -0.4981903938136796 1107 40-41 -0.5676365219519326 1107 42-43 -0.45573750164909654 1107 44-45 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0.2000975322448113 2213 100-101 0.18403296165201155 2214 1 -0.018849263844305852 2214 2 -0.32375516091250844 2214 3 -0.06626989699861241 2214 4 -0.04000888752280929 2214 5 -0.58489026442507 2214 6 -0.130484094206345 2214 7 0.03250413392160567 2214 8 -0.0446949643694694 2214 9 -0.13482263486515933 2214 10-11 -0.3965305095292777 2214 12-13 -0.27579701013701197 2214 14-15 0.10644656883164316 2214 16-17 -0.15637851374377476 2214 18-19 0.0065866519011805735 2214 20-21 -0.3399558884159717 2214 22-23 -0.5521151020182486 2214 24-25 -0.1053811630836492 2214 26-27 -0.1046023136285541 2214 28-29 -0.4446550146074273 2214 30-31 0.08148414883464028 2214 32-33 0.2906581059271147 2214 34-35 0.08978741983643346 2214 36-37 0.1252003483838635 2214 38-39 0.2487040209745004 2214 40-41 -0.02485911538276042 2214 42-43 0.2817535726108318 2214 44-45 0.17752413774493192 2214 46-47 0.1956228247150591 2214 48-49 0.18237234736230334 2214 50-51 0.32014007142318235 2214 52-53 0.23202470068926573 2214 54-55 0.2802819046853031 2214 56-57 0.267835894683202 2214 58-59 0.1560151006981485 2214 60-61 0.13222632893234731 2214 62-63 0.27457825730980545 2214 64-65 0.29940054794788296 2214 66-67 0.40497698406336724 2214 68-69 0.5598184328875817 2214 70-71 0.4793604045449058 2214 72-73 0.5217546967397624 2214 74-75 0.5460532851813156 2214 76-77 0.5421924279054764 2214 78-79 0.5274493168246579 2214 80-81 0.5138760392054174 2214 82-83 0.5230578198846167 2214 84-85 0.4205007267618903 2214 86-87 0.4113196188991388 2214 88-89 0.5276693132334636 2214 90-91 0.43186986548049333 2214 92-93 0.3478771790734996 2214 94-95 0.3544084487708119 2214 96-97 0.4442552650670848 2214 98-99 0.4991418448989364 2214 100-101 0.5736503930679113 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 100.0 11 2348.0 12 10612.0 13 22084.0 14 31876.0 15 38103.0 16 41224.0 17 42666.0 18 43614.0 19 44053.0 20 45837.0 21 48498.0 22 55772.0 23 67415.0 24 83083.0 25 98220.0 26 115695.0 27 135040.0 28 159825.0 29 191095.0 30 230123.0 31 281957.0 32 352926.0 33 450198.0 34 596808.0 35 826640.0 36 1182585.0 37 1599488.0 38 1457476.0 39 316882.0 40 152.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.80947187013434 19.17192601800227 13.195783771244871 24.822818340618515 2 15.502654001172916 19.90272086481964 38.89094595250813 25.703679181499318 3 18.698659991487933 24.327270061547893 29.157692926369688 27.816377020594484 4 13.480655061965052 16.601004092707 36.594285855729304 33.324054989598636 5 14.380083359181459 37.26609295308708 34.223703467975895 14.130120219755563 6 32.24111323952127 36.23980111957148 17.427876272968078 14.091209367939175 7 28.663286922414933 31.82008175641553 21.226861862315534 18.289769458854003 8 26.08586606230666 35.72377966710272 19.596788474231293 18.59356579635933 9 25.371308368854233 17.417516358475343 20.587534249691846 36.623641022978575 10-11 24.853438176195223 26.425369511944314 27.860371770959112 20.86082054090135 12-13 25.096056482477497 23.572213259536568 28.08018456657613 23.251545691409802 14-15 22.403684989533506 25.55830337698543 25.26615971577898 26.77185191770209 16-17 22.728199209640007 28.868201503413065 25.78953453791032 22.614064749036608 18-19 22.747626447109976 28.142847801482475 27.241958461781753 21.867567289625807 20-21 23.42198046162783 27.182732431204865 27.121453007121794 22.273834100045516 22-23 23.49654583087921 26.864306036309944 26.881975151588932 22.757172981221913 24-25 23.147247613790327 27.06826378036853 26.810354932589124 22.974133673252016 26-27 22.959614497916085 27.226600880346023 26.836117576619063 22.977667045118828 28-29 23.001332407148116 27.104706623404507 27.044791173483635 22.849169795963746 30-31 23.074562604340763 27.15927852507731 26.889134305452007 22.877024565129926 32-33 22.946233089569287 27.17539812305418 26.995777291867313 22.882591495509217 34-35 23.120491359269167 27.112560858648 26.818319710744724 22.94862807133811 36-37 23.271714863202305 27.013215806613555 26.702787456669547 23.012281873514592 38-39 23.004527904363933 27.237505343090657 26.849795502694317 22.908171249851094 40-41 23.0725908723874 27.089094135223927 26.878740589957058 22.959574402431613 42-43 23.13014671729723 27.11780214942097 26.763253672890418 22.988797460391382 44-45 22.953422532643007 27.191019730176112 26.8333906739134 23.022167063267485 46-47 23.156466794388187 26.9792635690115 26.779054137451595 23.085215499148717 48-49 23.129316105224813 27.02652961879587 26.776752961792088 23.06740131418723 50-51 22.99340594313307 27.128420596268693 26.85875599959539 23.01941746100285 52-53 23.043431138200294 27.1082863570453 26.78291827485034 23.065364229904066 54-55 23.069586248891618 27.175813844638018 26.829578021736744 22.925021884733617 56-57 23.026582197407052 27.04931927254519 26.821988186856675 23.10211034319107 58-59 23.033513457511525 26.82720492668173 26.909119504046373 23.23016211176037 60-61 23.152623663771355 26.96476021725133 26.82955713464905 23.053058984328263 62-63 22.98381858635737 27.10218350881898 26.854178280852913 23.059819623970736 64-65 23.073108369396962 26.997544941111546 26.768863074683434 23.160483614808054 66-67 22.972921024325853 26.92363266505895 26.825970154425416 23.277476156189785 68-69 23.022182785433333 27.040344888873747 26.80545852393157 23.132013801761346 70-71 23.031038837188277 26.847669681212505 26.855258054261828 23.26603342733739 72-73 22.94962749666019 26.94349048678304 26.891277370470227 23.215604646086536 74-75 22.95183385970003 26.979393703503934 26.90683525334963 23.16193718344641 76-77 23.033469814287226 26.898279985267738 26.81425146562762 23.253998734817415 78-79 23.039810317829872 26.888074148908952 26.898714719420873 23.173400813840303 80-81 22.891893615359137 26.904237475671934 26.98056105708569 23.22330785188324 82-83 22.916579126748466 26.835163501223224 27.034672810801723 23.213584561226586 84-85 23.021073059719072 26.81098439415325 26.84020447756936 23.32773806855832 86-87 22.960041251667363 26.88565129113388 26.91637348210779 23.237933975090968 88-89 22.93006552221918 26.82968594955576 26.92804586068267 23.312202667542387 90-91 22.89979220782879 26.751041601386877 26.977681294737927 23.37148489604641 92-93 22.976391631812128 26.85281848492989 26.92772097496236 23.243068908295623 94-95 23.011631870455442 26.60419009743385 27.002389182776504 23.381788849334203 96-97 23.047508616506057 26.62005598609319 26.978381533801155 23.3540538635996 98-99 22.907323467858184 26.736045972249645 26.983757672775155 23.372872887117012 100-101 22.988982236494564 26.5590162057757 26.931702516806116 23.520299040923618 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3965.0 1 2409.0 2 664.5 3 374.0 4 226.0 5 146.0 6 103.5 7 110.0 8 143.5 9 148.0 10 105.5 11 71.0 12 70.5 13 88.0 14 135.0 15 217.0 16 365.0 17 669.5 18 1211.5 19 1991.5 20 3275.5 21 5340.0 22 8411.5 23 13296.0 24 20150.5 25 28443.0 26 38977.5 27 52363.5 28 67760.0 29 84245.5 30 99812.5 31 115519.0 32 132387.5 33 147934.0 34 163517.5 35 181528.5 36 201793.0 37 223124.5 38 245288.5 39 263765.5 40 279001.5 41 293941.0 42 303925.5 43 310033.0 44 313229.0 45 314004.5 46 315189.5 47 309924.5 48 301157.0 49 296462.5 50 292298.5 51 288662.0 52 280648.5 53 273346.5 54 267159.0 55 253726.5 56 236627.0 57 215841.5 58 195872.5 59 180855.5 60 164263.0 61 144307.0 62 125343.5 63 107287.0 64 89954.0 65 74199.5 66 58818.5 67 45711.5 68 34991.0 69 26113.5 70 19530.5 71 14308.5 72 10695.0 73 7933.5 74 5924.5 75 4335.0 76 2925.5 77 1974.5 78 1355.0 79 944.5 80 626.5 81 354.0 82 191.0 83 93.0 84 49.0 85 20.0 86 14.0 87 13.5 88 7.0 89 5.0 90 7.0 91 4.5 92 1.0 93 1.0 94 1.5 95 4.5 96 4.5 97 1.0 98 2.5 99 4.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42934325821430297 2 0.06657416043007817 3 0.06687745956643389 4 0.0892166074941717 5 0.07386500505401349 6 9.332281118637207E-5 7 0.004036211583810592 8 2.449723793642267E-4 9 0.013415154108040986 10-11 8.632360034739416E-4 12-13 0.008696519467430048 14-15 0.00881900565711216 16-17 0.015911539307276436 18-19 0.008148247951710113 20-21 0.002175588035782299 22-23 0.010982928341496164 24-25 0.03376535962236924 26-27 0.00230390690116356 28-29 0.0036804183661625486 30-31 0.001423172870592174 32-33 0.0019947750891087033 34-35 0.0020356038190027406 36-37 5.424388400207876E-4 38-39 0.11589526614207582 40-41 0.0074133308136174305 42-43 0.004263685936077374 44-45 0.009378942524230393 46-47 0.007244183218342131 48-49 0.010936266935902977 50-51 0.013905098866769439 52-53 0.010172186419314557 54-55 0.009104806766370425 56-57 0.00881900565711216 58-59 0.014826661627234861 60-61 0.01848374929060082 62-63 0.009349779145734653 64-65 0.01536910046725565 66-67 0.012137798129927517 68-69 0.006888390000694088 70-71 8.165745978807556E-4 72-73 0.009262289010247428 74-75 0.0160281928212594 76-77 0.02438641709813885 78-79 0.016634791093970824 80-81 0.026882802297374307 82-83 0.05686858806669548 84-85 0.034371957895080664 86-87 0.3465775900433893 88-89 0.6084122348538535 90-91 0.5993774201958729 92-93 0.6064116270890457 94-95 0.598205052380344 96-97 0.592768998628738 98-99 0.6019612955305955 100-101 0.5951603956653887 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8572395.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.729707641198395 #Duplication Level Percentage of deduplicated Percentage of total 1 73.35013452257255 37.94381014295252 2 14.142322584084862 14.631564252844548 3 4.851332655538828 7.528740598236667 4 2.3103650962764064 4.780580438992309 5 1.3107108051361258 3.3901343375925777 6 0.8643253275800344 2.6826777901558936 7 0.5545390567043431 2.008030029525913 8 0.42771457031155513 1.7700439740878038 9 0.3178421758669142 1.4797694550274054 >10 1.7227550369885214 16.09756166498787 >50 0.10561457604400137 3.73239385919421 >100 0.041458178827811706 3.5759077356627658 >500 7.719614048889223E-4 0.2561262748184043 >1k 1.1345266307508965E-4 0.12265944592111802 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.166535139829651E-5 0.0 3 0.0 0.0 0.0 1.166535139829651E-5 0.0 4 0.0 0.0 0.0 1.166535139829651E-5 0.0 5 0.0 1.166535139829651E-5 0.0 1.166535139829651E-5 0.0 6 0.0 1.166535139829651E-5 0.0 1.166535139829651E-5 0.0 7 0.0 1.166535139829651E-5 0.0 2.333070279659302E-5 0.0 8 0.0 2.333070279659302E-5 0.0 2.333070279659302E-5 0.0 9 3.4996054194889524E-5 2.333070279659302E-5 0.0 2.333070279659302E-5 0.0 10-11 5.249408129233429E-5 2.333070279659302E-5 0.0 3.4996054194889524E-5 0.0 12-13 5.832675699148254E-5 2.333070279659302E-5 0.0 3.4996054194889524E-5 0.0 14-15 5.832675699148254E-5 6.41594326906308E-5 0.0 4.0828729894037784E-5 0.0 16-17 5.832675699148254E-5 9.332281118637207E-5 0.0 4.666140559318604E-5 0.0 18-19 6.41594326906308E-5 1.2248618968211332E-4 0.0 5.832675699148254E-5 0.0 20-21 6.999210838977905E-5 1.8081294667359588E-4 0.0 6.999210838977905E-5 0.0 22-23 6.999210838977905E-5 1.9831097377104065E-4 0.0 7.58247840889273E-5 0.0 24-25 6.999210838977905E-5 1.9831097377104065E-4 0.0 1.399842167795581E-4 0.0 26-27 6.999210838977905E-5 1.9831097377104065E-4 0.0 3.5579321764804355E-4 0.0 28-29 6.999210838977905E-5 2.041436494701889E-4 0.0 0.0013590134379015432 0.0 30-31 6.999210838977905E-5 2.0997632516933716E-4 0.0 0.004112036367899519 0.0 32-33 6.999210838977905E-5 2.0997632516933716E-4 0.0 0.009402273227026987 0.0 34-35 7.58247840889273E-5 2.1580900086848543E-4 0.0 0.018262107614033185 0.0 36-37 8.165745978807557E-5 2.508050550633749E-4 0.0 0.033392068377623756 0.0 38-39 8.165745978807557E-5 2.916337849574127E-4 0.0 0.054640505949620846 5.832675699148255E-6 40-41 8.165745978807557E-5 2.916337849574127E-4 0.0 0.07692132712036717 1.166535139829651E-5 42-43 8.165745978807557E-5 3.1496448775400574E-4 0.0 0.10036868343094316 1.166535139829651E-5 44-45 8.165745978807557E-5 3.20797163453154E-4 0.0 0.1295670579808793 1.166535139829651E-5 46-47 8.165745978807557E-5 3.499605419488953E-4 0.0 0.16805105224385952 1.166535139829651E-5 48-49 8.165745978807557E-5 3.5579321764804355E-4 0.0 0.2112361831203532 1.166535139829651E-5 50-51 8.165745978807557E-5 4.316180017369708E-4 0.0 0.2554187015414012 1.166535139829651E-5 52-53 9.332281118637207E-5 4.316180017369708E-4 0.0 0.2991054425280216 1.166535139829651E-5 54-55 9.332281118637207E-5 4.6078138023271213E-4 0.0 0.34961641408264554 1.166535139829651E-5 56-57 9.332281118637207E-5 5.016101101267499E-4 0.0 0.41251598882226026 1.166535139829651E-5 58-59 9.332281118637207E-5 5.366061643216394E-4 0.0 0.48379711854155116 1.166535139829651E-5 60-61 9.332281118637207E-5 5.366061643216394E-4 0.0 0.5617158332064727 1.166535139829651E-5 62-63 9.332281118637207E-5 5.599368671182324E-4 0.0 0.641361019878342 1.166535139829651E-5 64-65 9.332281118637207E-5 5.599368671182324E-4 0.0 0.727124683358618 1.166535139829651E-5 66-67 9.332281118637207E-5 5.832675699148255E-4 0.0 0.8302988838008515 1.166535139829651E-5 68-69 9.332281118637207E-5 5.891002456139738E-4 0.0 0.9493437948204673 1.166535139829651E-5 70-71 9.332281118637207E-5 6.065982727114184E-4 0.0 1.0775343413363476 1.166535139829651E-5 72-73 9.332281118637207E-5 6.182636241097149E-4 0.0 1.212251651959575 1.166535139829651E-5 74-75 9.332281118637207E-5 6.240962998088633E-4 0.0 1.3517459239804044 1.166535139829651E-5 76-77 9.332281118637207E-5 6.707577054020492E-4 0.0 1.5117945451650328 1.166535139829651E-5 78-79 9.332281118637207E-5 6.765903811011975E-4 0.0 1.6934298991122083 1.166535139829651E-5 80-81 9.332281118637207E-5 6.940884081986422E-4 0.0 1.8923824672101555 1.166535139829651E-5 82-83 1.0498816258466858E-4 7.11586435296087E-4 0.0 2.103035382760594 1.166535139829651E-5 84-85 1.0498816258466858E-4 7.290844623935317E-4 0.0 2.3167504530530847 1.166535139829651E-5 86-87 1.0498816258466858E-4 7.524151651901248E-4 0.0 2.547864394956135 1.166535139829651E-5 88-89 1.0498816258466858E-4 7.58247840889273E-4 0.0 2.8069401841609025 1.166535139829651E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7290 0.0 39.406097 1 GTATCAA 11845 0.0 32.296406 1 TCAACGC 11705 0.0 30.98226 4 ATCAACG 12085 0.0 30.047335 3 CAACGCA 12260 0.0 29.729553 5 AACGCAG 12785 0.0 28.527386 6 TATCAAC 14330 0.0 25.736578 2 GTACATG 28980 0.0 22.636497 1 ACGCAGA 15965 0.0 22.60919 7 TACATGG 29265 0.0 21.376806 2 CGCAGAG 17140 0.0 20.509508 8 ACATGGG 30170 0.0 20.04404 3 CATGGGG 25005 0.0 18.147724 4 GTATATA 4725 0.0 16.333786 1 GCAGAGT 21180 0.0 16.171825 9 GTGGTAT 4740 0.0 16.081083 1 CAGAGTA 19570 0.0 15.480006 10-11 GAGTACA 21205 0.0 15.434485 1 TATACTG 4565 0.0 14.866642 5 GTATAGA 3215 0.0 14.818105 1 >>END_MODULE