FastQCFastQC Report
Wed 25 May 2016
SRR2049394_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049394_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8572395
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82250.041.898171
GTATCAA130850.034.32821
TCAACGC139050.031.1888084
ATCAACG143950.030.1931513
CAACGCA144450.029.9250935
AACGCAG153500.028.1910676
TATCAAC166950.026.2896332
ACGCAGA191550.022.5167267
GTACATG292550.021.0245691
CGCAGAG207800.020.5959018
TACATGG296850.019.8739192
ACATGGG301600.018.8994393
TAGACCG4450.018.1468225
GTGGTAT52850.017.1783451
GCAGAGT249750.017.098419
CATGGGG253200.016.4526124
CAGAGTA232400.016.08570710-11
AGAGTAC216950.015.56723510-11
GAGTACA216500.014.9294541
CCTAGAC23400.014.4125723
Produced by FastQC (version 0.11.5)