FastQCFastQC Report
Wed 25 May 2016
SRR2049393_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049393_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2625707
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38000.036.520781
GGTATCA23700.036.448451
TCAACGC42400.031.4003354
ATCAACG43450.030.7497983
CAACGCA47250.028.1729265
AACGCAG48550.027.663776
TATCAAC49600.027.1278062
GTACATG103100.025.3187791
TACATGG107250.024.214312
ACGCAGA58600.022.9180767
ACATGGG109600.022.7069513
CGCAGAG63900.020.870238
CATGGGG93650.020.2949184
GTATAAC6500.018.155281
GTACCGA1600.001286379817.6102646
GCAGAGT75250.017.5975559
GAGTACA70650.017.438321
AGAGTAC62450.016.95569210-11
CCTAGAC6400.016.9068053
TACTCCG5250.016.1272585
Produced by FastQC (version 0.11.5)