##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049393_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2625707 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.43618537788108 33.0 31.0 34.0 30.0 34.0 2 31.67638430335144 34.0 31.0 34.0 30.0 34.0 3 31.915140569758925 34.0 31.0 34.0 30.0 34.0 4 35.38070584417835 37.0 35.0 37.0 33.0 37.0 5 35.4609691789678 37.0 37.0 37.0 35.0 37.0 6 35.501594808560135 37.0 37.0 37.0 35.0 37.0 7 35.42469552010182 37.0 37.0 37.0 35.0 37.0 8 35.443506453690375 37.0 37.0 37.0 35.0 37.0 9 37.160680152050475 39.0 38.0 39.0 35.0 39.0 10-11 37.163777793942735 39.0 39.0 39.0 35.0 39.0 12-13 37.1218978355163 39.0 38.5 39.0 35.0 39.0 14-15 38.63983624220067 41.0 40.0 41.0 35.5 41.0 16-17 38.62588076278122 41.0 40.0 41.0 35.0 41.0 18-19 38.58136989389905 41.0 39.5 41.0 35.0 41.0 20-21 38.607338899580185 41.0 40.0 41.0 35.0 41.0 22-23 38.5602268265271 41.0 39.0 41.0 35.0 41.0 24-25 38.56963438799531 41.0 39.0 41.0 35.0 41.0 26-27 38.56627681611086 41.0 39.0 41.0 35.0 41.0 28-29 38.533954283551054 41.0 39.0 41.0 35.0 41.0 30-31 38.451216186726086 41.0 39.0 41.0 34.0 41.0 32-33 38.43022107950354 41.0 39.0 41.0 34.0 41.0 34-35 38.374771061660724 41.0 39.0 41.0 34.0 41.0 36-37 38.248790516230486 41.0 39.0 41.0 34.0 41.0 38-39 38.14409281005078 41.0 39.0 41.0 33.5 41.0 40-41 38.03898683287967 41.0 38.0 41.0 33.0 41.0 42-43 37.9119821823227 40.5 38.0 41.0 33.0 41.0 44-45 37.732451107454104 40.0 38.0 41.0 33.0 41.0 46-47 37.58648832485879 40.0 38.0 41.0 33.0 41.0 48-49 37.4442270215222 40.0 37.0 41.0 33.0 41.0 50-51 37.257132459943165 40.0 37.0 41.0 32.0 41.0 52-53 37.068413002669374 40.0 36.5 41.0 32.0 41.0 54-55 36.876914293940644 40.0 36.0 41.0 31.5 41.0 56-57 36.605381712430216 39.0 35.0 41.0 31.0 41.0 58-59 36.36893206286916 39.0 35.0 41.0 31.0 41.0 60-61 36.13321554918352 39.0 35.0 41.0 31.0 41.0 62-63 35.79894691220308 38.0 35.0 40.0 30.0 41.0 64-65 35.5716774567764 37.5 35.0 40.0 30.0 41.0 66-67 35.33830145556987 37.0 35.0 40.0 30.0 41.0 68-69 35.09454710674116 36.5 35.0 39.5 30.0 41.0 70-71 34.87063084342617 36.0 35.0 39.0 30.0 41.0 72-73 34.54619098779872 35.5 35.0 39.0 30.0 41.0 74-75 34.190775665373174 35.0 34.5 38.0 29.5 40.5 76-77 33.87045965143864 35.0 34.0 37.0 29.0 39.0 78-79 33.5749392449348 35.0 34.0 37.0 29.0 39.0 80-81 33.27244090829632 35.0 34.0 36.0 29.0 39.0 82-83 33.004275039065675 35.0 34.0 36.0 29.0 37.0 84-85 32.76181443702592 35.0 34.0 35.5 29.0 37.0 86-87 32.52991670433906 35.0 34.0 35.0 28.5 36.5 88-89 32.24404741275397 35.0 34.0 35.0 27.0 36.0 90-91 32.04018936614024 35.0 34.0 35.0 27.0 36.0 92-93 31.881517435113665 35.0 34.0 35.0 26.5 36.0 94-95 31.742260465467012 35.0 33.5 35.0 26.0 35.5 96-97 31.588844452179927 35.0 33.0 35.0 25.0 35.0 98-99 31.42835529630686 35.0 33.0 35.0 25.0 35.0 100 31.281964819380075 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1775597984661026 1101 2 -0.21444767837222045 1101 3 -0.04847167915247752 1101 4 0.08554040305509858 1101 5 0.06962914491509764 1101 6 0.07336151214681053 1101 7 0.05000870341708463 1101 8 0.03683674421220928 1101 9 0.12191492467895415 1101 10-11 0.11380122315679131 1101 12-13 0.13125743598148887 1101 14-15 0.13613718558108445 1101 16-17 0.10871137035613998 1101 18-19 0.057051686502418875 1101 20-21 0.07820944334513058 1101 22-23 0.0932274963514601 1101 24-25 0.06517853254094064 1101 26-27 0.09862436717934031 1101 28-29 0.08445263281608106 1101 30-31 0.03987662324021102 1101 32-33 0.054848437687518015 1101 34-35 0.03692653044344496 1101 36-37 0.06764626709178145 1101 38-39 0.17558279299598212 1101 40-41 0.07135845971207289 1101 42-43 0.07374515848810148 1101 44-45 -0.043080184802725796 1101 46-47 -0.08618953411033203 1101 48-49 -0.11764516615788523 1101 50-51 -0.02309613027213686 1101 52-53 -0.06132497486104427 1101 54-55 -0.050518042669608576 1101 56-57 -0.07112591066720597 1101 58-59 -0.018009745814296707 1101 60-61 -0.2007740542763159 1101 62-63 -0.08811051272056858 1101 64-65 -0.07385367305081303 1101 66-67 -0.011027361823231274 1101 68-69 0.0033734879020315134 1101 70-71 -0.09418296826521555 1101 72-73 -0.16210565650114006 1101 74-75 -0.17581889589877875 1101 76-77 -0.12064928638189798 1101 78-79 -0.18981106236658718 1101 80-81 -0.18325226211061363 1101 82-83 -0.1746739029935256 1101 84-85 -0.235400183916731 1101 86-87 -0.24280124645738255 1101 88-89 -0.1940994808871963 1101 90-91 -0.17105771200160547 1101 92-93 -0.08571253489395048 1101 94-95 -0.10612314122793975 1101 96-97 -0.0706926700546795 1101 98-99 -0.06348382713478884 1101 100 -0.1065134461771251 1102 1 -0.1038294710512524 1102 2 -0.189268733808845 1102 3 -0.1296653005650974 1102 4 -0.03450322435757158 1102 5 0.1604895521412928 1102 6 0.0960399944287289 1102 7 0.1266802939685192 1102 8 0.12961332411516224 1102 9 0.053846082534938944 1102 10-11 0.12057534410670456 1102 12-13 0.22658978112146855 1102 14-15 0.18568397892781974 1102 16-17 0.14942160550260297 1102 18-19 0.11004601058286312 1102 20-21 0.07241971500434374 1102 22-23 0.05722488793097824 1102 24-25 0.09536869356863065 1102 26-27 0.09262884351586109 1102 28-29 0.054698874030066236 1102 30-31 0.09988084249272333 1102 32-33 0.1274324778951197 1102 34-35 0.07570107673500814 1102 36-37 0.13253414301843236 1102 38-39 0.09639929522710133 1102 40-41 0.12338141620381293 1102 42-43 0.13339988014341486 1102 44-45 0.05002603487940149 1102 46-47 -0.023283553832349924 1102 48-49 0.03648417101376822 1102 50-51 -0.00896129269840884 1102 52-53 -0.11034602713750274 1102 54-55 -0.12531377405012734 1102 56-57 -0.13167461847841366 1102 58-59 -0.052167328063035256 1102 60-61 -0.10547472008973813 1102 62-63 -0.36104434946026487 1102 64-65 -0.17730632838271987 1102 66-67 -0.12842729443476486 1102 68-69 -0.1538324872086534 1102 70-71 0.02336959196269106 1102 72-73 0.04738164026049674 1102 74-75 0.032631438417197955 1102 76-77 -0.028025590325235328 1102 78-79 -0.033115582646544794 1102 80-81 -0.056778374287219435 1102 82-83 -0.012387102292990448 1102 84-85 0.05313886573507176 1102 86-87 0.025978932621704587 1102 88-89 0.046560832131802954 1102 90-91 0.07680915264536736 1102 92-93 -5.204030312100372E-4 1102 94-95 0.058475024080493654 1102 96-97 -0.05503206877703093 1102 98-99 -0.1978056100508354 1102 100 -0.10837982484137143 1103 1 -0.24476887352911803 1103 2 -0.30139763055312585 1103 3 -0.2093394725469615 1103 4 -0.170010452172491 1103 5 -0.007368231101111178 1103 6 -0.0657383981188957 1103 7 -0.09755529949784147 1103 8 -0.03362514681561635 1103 9 -0.057323090172694435 1103 10-11 0.027731435878173727 1103 12-13 -0.035636881105197915 1103 14-15 -0.09027595857057946 1103 16-17 -0.08257289572267013 1103 18-19 -0.12269987600917887 1103 20-21 -0.07175235224464416 1103 22-23 -0.08883930428706321 1103 24-25 -0.03104079437932228 1103 26-27 -0.13107565711171532 1103 28-29 -0.0796599295595044 1103 30-31 -0.018353060356560036 1103 32-33 -0.00671286394253201 1103 34-35 0.007360869933265235 1103 36-37 0.009703981726737254 1103 38-39 -0.002230280325548506 1103 40-41 0.03480418147272957 1103 42-43 -0.07491573586450784 1103 44-45 -0.19013038989240982 1103 46-47 -0.264560225878256 1103 48-49 -0.1796970836290228 1103 50-51 -0.22161872074045874 1103 52-53 -0.22846295294673524 1103 54-55 -0.1691869948464273 1103 56-57 -0.08761013595239575 1103 58-59 -0.08055774106883007 1103 60-61 -0.1612343450801248 1103 62-63 -0.13403460706590664 1103 64-65 -0.11834306941945982 1103 66-67 -0.07157645296853588 1103 68-69 -0.029227177987962705 1103 70-71 -0.07382893600507145 1103 72-73 -0.09601598775251574 1103 74-75 -0.13957327994136648 1103 76-77 -0.117572795257054 1103 78-79 -0.07112885142904446 1103 80-81 -0.06288935732128209 1103 82-83 -0.10582699573288323 1103 84-85 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-0.12769080104657604 1104 40-41 0.010029939987248326 1104 42-43 -0.03487475975848753 1104 44-45 -0.14291710604704377 1104 46-47 -0.1102754678061828 1104 48-49 -0.09300965458575661 1104 50-51 -0.23319388285589326 1104 52-53 -0.22395820695273727 1104 54-55 -0.15267581086973792 1104 56-57 -0.1346316676107051 1104 58-59 -0.10389421344777361 1104 60-61 -0.15429854346154315 1104 62-63 -0.11154939996531255 1104 64-65 -0.20839488130715722 1104 66-67 -0.15770978755592324 1104 68-69 -0.17163780042425714 1104 70-71 -0.21434974325316603 1104 72-73 -0.22996905725709382 1104 74-75 -0.22563431066913608 1104 76-77 -0.19225300253595634 1104 78-79 -0.22674490186696517 1104 80-81 -0.2831192718381317 1104 82-83 -0.22795225109875616 1104 84-85 -0.24376721072594165 1104 86-87 -0.1891840221796386 1104 88-89 -0.11457105097504439 1104 90-91 -0.18134500968125167 1104 92-93 -0.1408142615448149 1104 94-95 -0.10593602461428375 1104 96-97 -0.15229819316416027 1104 98-99 -0.18530287557313585 1104 100 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56-57 -0.38463406297569236 1105 58-59 -0.3727150111005457 1105 60-61 -0.3214451884605296 1105 62-63 -0.22475797061896685 1105 64-65 -0.3131379680386104 1105 66-67 -0.26589378780035844 1105 68-69 -0.256484721512642 1105 70-71 -0.20958074131311122 1105 72-73 -0.24903574761336245 1105 74-75 -0.2547531596264747 1105 76-77 -0.2637420481735049 1105 78-79 -0.2517144505537914 1105 80-81 -0.24926842650062042 1105 82-83 -0.19427854960348157 1105 84-85 -0.14863195182093847 1105 86-87 -0.11593632597378445 1105 88-89 0.0012900374712572216 1105 90-91 -0.006805325273418816 1105 92-93 -0.059757901813050296 1105 94-95 -0.09865045599047306 1105 96-97 -0.10860933862769429 1105 98-99 -0.10189626670454999 1105 100 -0.0343612478122175 1106 1 -0.25238683390571737 1106 2 -0.30360805734295937 1106 3 -0.2696737906134494 1106 4 -0.18229157443133204 1106 5 -0.03298806599750037 1106 6 -0.1012669653018321 1106 7 -0.18160340862822721 1106 8 -0.1632423423785596 1106 9 -0.1285398664733819 1106 10-11 -0.23299229768841911 1106 12-13 -0.12743829432569242 1106 14-15 -0.13646204760301117 1106 16-17 -0.17243674107007223 1106 18-19 -0.07614508299595002 1106 20-21 -0.05955662943545548 1106 22-23 -0.12464552263993767 1106 24-25 -0.13029322534335108 1106 26-27 -0.168043135758289 1106 28-29 -0.08111961993352423 1106 30-31 -0.11973373845589208 1106 32-33 -0.09522226128898836 1106 34-35 -0.16819228150860255 1106 36-37 -0.20286955034433873 1106 38-39 -0.21071646968828617 1106 40-41 -0.151989237295993 1106 42-43 -0.20956359922718804 1106 44-45 -0.27465132219231947 1106 46-47 -0.2905750098296238 1106 48-49 -0.33980731144301046 1106 50-51 -0.3256125246565773 1106 52-53 -0.33179958170293133 1106 54-55 -0.35113855310370923 1106 56-57 -0.24577530799765412 1106 58-59 -0.2985062343806817 1106 60-61 -0.23191730930138732 1106 62-63 -0.27301631466103515 1106 64-65 -0.35169966764451743 1106 66-67 -0.3093317031627194 1106 68-69 -0.27463015294387105 1106 70-71 -0.3401744318523896 1106 72-73 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2213 60-61 0.31072095238530295 2213 62-63 0.3038927187873526 2213 64-65 0.3131234777027885 2213 66-67 0.282572648239686 2213 68-69 0.23845993724950887 2213 70-71 0.2773275828066275 2213 72-73 0.26887854005727263 2213 74-75 0.3422758165365849 2213 76-77 0.3484090126151358 2213 78-79 0.36711436681450493 2213 80-81 0.30845105431256314 2213 82-83 0.3241237313131009 2213 84-85 0.35306209263394805 2213 86-87 0.36749197603564454 2213 88-89 0.34134935321250737 2213 90-91 0.35157068140091496 2213 92-93 0.35389295616643324 2213 94-95 0.307252263737702 2213 96-97 0.2890993349656519 2213 98-99 0.3609552764118895 2213 100 0.31008325842307727 2214 1 0.13856907408701957 2214 2 0.06820028119441801 2214 3 -0.042154181547129355 2214 4 -0.12434291282740162 2214 5 -0.1689595375817703 2214 6 -0.08077710019658468 2214 7 -0.08689577988152308 2214 8 -0.07412661935080678 2214 9 -0.10155528930478397 2214 10-11 -0.18988147456929738 2214 12-13 -0.1507580612948729 2214 14-15 -0.1990725887054623 2214 16-17 -0.2064808634135673 2214 18-19 -0.1965374364115391 2214 20-21 -0.20507143919913773 2214 22-23 -0.18080511859218262 2214 24-25 -0.17593830993015303 2214 26-27 -0.18940282344730264 2214 28-29 -0.20989718440161198 2214 30-31 -0.21558101680474806 2214 32-33 -0.1977053979276846 2214 34-35 -0.2379906349019123 2214 36-37 -0.1739158757603505 2214 38-39 -0.22810109023218672 2214 40-41 -0.14755297664839162 2214 42-43 -0.12666851417154845 2214 44-45 -0.0975762106680449 2214 46-47 -0.09703377621271159 2214 48-49 -0.08441661769523989 2214 50-51 -0.05978459420663995 2214 52-53 -0.08106100470179456 2214 54-55 -0.11258571063713418 2214 56-57 -0.1363960005970597 2214 58-59 -0.16914433887566105 2214 60-61 -0.16798435657197075 2214 62-63 -0.14592050830903247 2214 64-65 -0.24414991097972916 2214 66-67 -0.24638969732867366 2214 68-69 -0.17968692196719616 2214 70-71 -0.22373847500838906 2214 72-73 -0.19103679062368428 2214 74-75 -0.16603724449805668 2214 76-77 -0.12569998594483422 2214 78-79 -0.16783360435086792 2214 80-81 -0.19948528685713995 2214 82-83 -0.21422544659708365 2214 84-85 -0.2635198940743706 2214 86-87 -0.20730992463975184 2214 88-89 -0.15028532019379526 2214 90-91 -0.10571372782271737 2214 92-93 -0.1184966429252583 2214 94-95 -0.10637010035367211 2214 96-97 -0.07988332102554452 2214 98-99 -0.09078827426637304 2214 100 -0.07528369722469819 2215 1 0.10895430066574718 2215 2 0.04014922474486582 2215 3 -0.07265577710279914 2215 4 -0.17307030974639304 2215 5 -0.1886919127409996 2215 6 -0.16339789183891895 2215 7 -0.14085350458593382 2215 8 -0.19921623591552162 2215 9 -0.24473727604440398 2215 10-11 -0.27186311999809476 2215 12-13 -0.26262448477216793 2215 14-15 -0.30306087023411976 2215 16-17 -0.2672078070161277 2215 18-19 -0.23442117697636178 2215 20-21 -0.2744790471741396 2215 22-23 -0.2436028051967014 2215 24-25 -0.21425468218669153 2215 26-27 -0.17303165733613213 2215 28-29 -0.2186321618435727 2215 30-31 -0.22289563464508433 2215 32-33 -0.22393232600628465 2215 34-35 -0.2055133130015392 2215 36-37 -0.18714391748577697 2215 38-39 -0.17426000336482161 2215 40-41 -0.18236553163256985 2215 42-43 -0.15007536617522277 2215 44-45 -0.09295920437613603 2215 46-47 -0.11331469106620773 2215 48-49 -0.08622274575771627 2215 50-51 -0.06812866321529043 2215 52-53 -0.06317764620132493 2215 54-55 -0.05851061975950245 2215 56-57 -0.09056120122703959 2215 58-59 -0.07541594184704081 2215 60-61 -0.10086264210689677 2215 62-63 -0.10862178817465917 2215 64-65 -0.02489901597942179 2215 66-67 -0.0947486477021755 2215 68-69 -0.10351762817111165 2215 70-71 -0.14915857723682535 2215 72-73 -0.15096375473618906 2215 74-75 -0.11981305579589474 2215 76-77 -0.1276971461828751 2215 78-79 -0.21267203982481675 2215 80-81 -0.18173648326377645 2215 82-83 -0.1646230890186473 2215 84-85 -0.13639441140598763 2215 86-87 -0.1401228888240169 2215 88-89 -0.1795479742858035 2215 90-91 -0.18786474262808284 2215 92-93 -0.22459757800070435 2215 94-95 -0.19987645889501238 2215 96-97 -0.26061790508274285 2215 98-99 -0.27619120191145896 2215 100 -0.19251598144594695 2216 1 0.5158270445590212 2216 2 0.3993932785525871 2216 3 0.24836187457190562 2216 4 0.13190737211082393 2216 5 0.10503825969577463 2216 6 0.06647681515456583 2216 7 0.11679198061199969 2216 8 0.07892060001203305 2216 9 0.10485660059747914 2216 10-11 0.035079593871067516 2216 12-13 0.04012271575266624 2216 14-15 0.021931257660646963 2216 16-17 0.07200708040021198 2216 18-19 0.09035696488226108 2216 20-21 0.07313996456852578 2216 22-23 0.07625499077610698 2216 24-25 0.05073490731634678 2216 26-27 0.05958327636625427 2216 28-29 0.00358993159164811 2216 30-31 0.05715465410180087 2216 32-33 1.3722979061725482E-4 2216 34-35 0.04998437211656892 2216 36-37 0.03948473898636706 2216 38-39 0.01884005292798463 2216 40-41 0.13134419844405443 2216 42-43 0.1290028962666625 2216 44-45 0.23079115928614868 2216 46-47 0.21386706048589588 2216 48-49 0.16117325841962327 2216 50-51 0.19083435160590767 2216 52-53 0.19857389045294127 2216 54-55 0.23239001862476982 2216 56-57 0.18704811671830157 2216 58-59 0.19426395420639864 2216 60-61 0.16833980834906725 2216 62-63 0.14268952103660837 2216 64-65 0.1199194387852387 2216 66-67 0.12840652684992193 2216 68-69 0.10825077314050446 2216 70-71 0.03485730142728016 2216 72-73 0.07032786551886261 2216 74-75 0.05495208501530158 2216 76-77 0.026445628885873873 2216 78-79 0.012970797254865829 2216 80-81 0.01729931424134179 2216 82-83 0.022161104798570364 2216 84-85 0.012923333033988627 2216 86-87 0.008117410713936124 2216 88-89 -0.010511543148659541 2216 90-91 -0.0029200134999811667 2216 92-93 0.013789917348475456 2216 94-95 0.0023914801356781368 2216 96-97 -0.03795961008607662 2216 98-99 -0.07414080343804841 2216 100 -0.021111601734997265 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33024.0 3 5044.0 4 3498.0 5 2931.0 6 2652.0 7 2769.0 8 2798.0 9 3052.0 10 3454.0 11 3827.0 12 4210.0 13 4552.0 14 4632.0 15 4901.0 16 4830.0 17 4976.0 18 5088.0 19 5234.0 20 5288.0 21 5708.0 22 6753.0 23 8259.0 24 10289.0 25 13235.0 26 16049.0 27 17240.0 28 19502.0 29 24317.0 30 27902.0 31 31784.0 32 40104.0 33 54406.0 34 80832.0 35 134909.0 36 284006.0 37 616947.0 38 828401.0 39 297705.0 40 599.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.991901728076385 18.308202396947305 13.668423568626217 24.03147230635009 2 16.096396972022024 18.903284128627575 39.24929183338865 25.75102706596175 3 19.531329963320946 23.385189666313778 28.25914264807202 28.824337722293258 4 13.669622026624213 15.61132101992767 35.63364282363942 35.0854141298087 5 15.196981501000565 36.57004720223638 33.52729786291493 14.70567343384813 6 34.14708664540836 34.748066155043254 16.75552690600064 14.349320293547747 7 30.287867127453396 30.561265388901305 20.303548723925342 18.847318759719958 8 26.963427947806395 34.989839719622786 18.958637039093343 19.088095293477476 9 26.286167778142612 16.39405092462824 19.523649897089797 37.79613140013935 10-11 25.978512935270924 25.66153974043089 26.85009865731108 21.509848666987104 12-13 26.0790757558454 22.7776399751307 27.195753839689107 23.947530429334794 14-15 23.366025781812414 24.656393749161147 24.14943964517979 27.828140823846653 16-17 23.85843201512946 27.95556864640318 24.67322289360477 23.51277644486259 18-19 23.961206346797535 27.175041384739192 26.034723335654782 22.829028932808495 20-21 24.69703726767169 26.189738496529984 25.856664913179507 23.256559322618823 22-23 24.6099683886727 25.985182202245 25.792452063907547 23.612397345174756 24-25 24.301005977909096 26.127094675171037 25.688593928289876 23.88330541862999 26-27 24.064476275636174 26.31033568921436 25.782655199302962 23.842532835846505 28-29 24.022599240616096 26.324450720316474 25.877453726354098 23.775496312713333 30-31 24.122241430982296 26.49093701551792 25.622672674848225 23.76414887865156 32-33 24.03949060565403 26.28024390718167 25.833107429805008 23.847158057359287 34-35 24.197445107010495 26.178503198858394 25.82003509765849 23.80401659647262 36-37 24.291053091829998 26.218581883131975 25.66848304454495 23.82188198049308 38-39 24.134806691762332 26.324790169951424 25.683914710321204 23.85648842796504 40-41 24.131537215585407 26.232527568635888 25.795081765650817 23.84085345012788 42-43 24.207552183914004 26.205462044059175 25.75113686145789 23.83584891056893 44-45 24.001329045009104 26.33869068860239 25.76595464014372 23.89402562624479 46-47 24.16566153146955 26.193070219536015 25.788118453634052 23.853149795360384 48-49 24.189194186605526 26.219006114045225 25.594505784839132 23.997293914510117 50-51 24.047270481397184 26.3421324518965 25.730521275338447 23.880075791367865 52-53 24.12787239753311 26.24375880705907 25.682947398600632 23.94542139680719 54-55 24.158389304732097 26.28640174279247 25.744447879974853 23.81076107250058 56-57 24.08280420879901 26.21204276510683 25.800625722963176 23.90452730313098 58-59 24.098217681355855 26.05805331800648 25.85546815055012 23.98826085008755 60-61 24.211396397220746 26.18170442691653 25.628759249431155 23.978139926431567 62-63 23.990543731619194 26.311565399025206 25.73826816691484 23.95962270244076 64-65 24.1664050349195 26.114718039132484 25.708440630943308 24.010436295004713 66-67 24.025177303747693 26.125579971320278 25.735913974558848 24.113328750373174 68-69 24.06581847420671 26.189432275818735 25.75823197965924 23.98651727031531 70-71 24.079467764547807 26.012077404280774 25.784186744518824 24.12426808665259 72-73 24.126822279999903 26.172824488957026 25.754865244273173 23.945487986769898 74-75 24.007230335375446 26.216372362271834 25.749108125165648 24.027289177187072 76-77 24.099734451475197 26.115874784023774 25.637433705807922 24.146957058693104 78-79 24.13352184445011 26.131114362266967 25.805185352106662 23.93017844117626 80-81 23.963317703644474 26.12506877787577 25.878307295926557 24.033306222553193 82-83 24.037713843221574 26.200514068247966 25.781420529597433 23.980351558933027 84-85 24.1143017510251 26.066962701752626 25.68870482711575 24.13003072010652 86-87 24.1055932591124 26.302710843373493 25.672334909257284 23.919360988256823 88-89 23.966755234203298 26.18249746641598 25.76674606476806 24.08400123461266 90-91 23.98064802809318 26.11053580700237 25.765359739951883 24.143456424952564 92-93 24.07007481794709 26.27248877577195 25.690253545442953 23.967182860838005 94-95 24.07189340658328 26.080083327956345 25.804743118223072 24.043280147237304 96-97 24.117235714240444 26.164908145037362 25.698954489958304 24.018901650763887 98-99 24.007173205997468 26.337946582394107 25.694186491832095 23.96069371977633 100 24.283024803871143 25.986079777571458 25.560613737451803 24.170281681105596 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2520.0 1 1531.5 2 531.0 3 450.5 4 328.0 5 252.5 6 233.0 7 227.5 8 243.0 9 254.5 10 218.0 11 173.0 12 175.5 13 200.0 14 191.5 15 188.5 16 225.0 17 276.0 18 354.5 19 463.0 20 661.5 21 974.0 22 1429.5 23 2228.5 24 3424.0 25 4990.5 26 7189.0 27 10028.5 28 13546.0 29 17897.5 30 22189.0 31 26479.5 32 31353.0 33 35917.0 34 40282.5 35 45483.5 36 51085.5 37 57001.0 38 63423.5 39 68653.0 40 73359.0 41 78409.0 42 81738.0 43 83855.0 44 86969.5 45 89478.5 46 91363.0 47 91567.5 48 91342.0 49 92513.5 50 92611.5 51 92943.5 52 92278.0 53 92645.0 54 93288.0 55 90771.5 56 86177.0 57 79625.5 58 73237.0 59 68926.5 60 64167.0 61 56938.0 62 49995.5 63 43427.0 64 36459.0 65 30641.0 66 25115.0 67 20077.0 68 15856.5 69 12052.0 70 9042.5 71 6775.0 72 5246.0 73 3986.5 74 3082.0 75 2310.5 76 1585.0 77 1110.0 78 792.5 79 569.0 80 382.5 81 233.5 82 155.0 83 104.5 84 80.5 85 67.5 86 58.5 87 49.0 88 37.0 89 29.0 90 23.0 91 18.5 92 18.0 93 17.0 94 15.5 95 9.5 96 8.0 97 9.0 98 9.0 99 11.5 100 19.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7321837508907125 2 0.27901056743955055 3 0.2787439725757672 4 0.29093116634872057 5 0.27371675514442395 6 0.15184481741489056 7 0.13424955640518915 8 0.12339533695115258 9 0.13398296154140582 10-11 0.12360480434412521 12-13 0.12173864029764174 14-15 0.11741599500629735 16-17 0.11461674893657213 18-19 0.11659716792467704 20-21 0.1261945830208778 22-23 0.12901287158087327 24-25 0.11937737150413204 26-27 0.1215672578852096 28-29 0.12636596543330994 30-31 0.1165781254344068 32-33 0.11067495345063252 34-35 0.10435284668091299 36-37 0.1141216441895459 38-39 0.10703783780901678 40-41 0.10486699391820946 42-43 0.11105580325603732 44-45 0.10553348107766784 46-47 0.11141761057117189 48-49 0.10397199687550819 50-51 0.10347689212848196 52-53 0.10705688029928702 54-55 0.10625709570793694 56-57 0.10743773010469182 58-59 0.10890400185550025 60-61 0.10827559967658235 62-63 0.11031314613549799 64-65 0.10456231407388562 66-67 0.11227452263333265 68-69 0.10648560559117982 70-71 0.11265537243873745 72-73 0.12196715018088461 74-75 0.1303268034095198 76-77 0.11511185368359837 78-79 0.11615919064846154 80-81 0.1193583290138618 82-83 0.11057974099928133 84-85 0.12025332605656305 86-87 0.11071303843117303 88-89 0.7320504534588208 90-91 0.7923389776543993 92-93 0.8195506962505718 94-95 0.8385931865208113 96-97 0.8506851678424135 98-99 0.8534082439510577 100 0.8547793032505149 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2625707.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.3551371780267 #Duplication Level Percentage of deduplicated Percentage of total 1 80.45207902697193 50.97052503011251 2 11.249697821452322 14.254522973789202 3 3.4134753663097137 6.4878360025910045 4 1.5671448449803167 3.971467065262615 5 0.8742694092458335 2.7694729176661075 6 0.5330674890539209 2.026353833649444 7 0.3601788169573143 1.5973424849865012 8 0.26147628557957975 1.325269275335613 9 0.19283917863166267 1.0995617353956286 >10 1.03041979194991 11.595976680262302 >50 0.04864936498880518 2.068016718420073 >100 0.016401301372064917 1.669145749315907 >500 2.4104200532511737E-4 0.08716065545291346 >1k 6.026050132323613E-5 0.07734887776022077 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.808498054047919E-5 0.0 4 0.0 3.808498054047919E-5 0.0 3.808498054047919E-5 0.0 5 0.0 3.808498054047919E-5 0.0 3.808498054047919E-5 0.0 6 0.0 3.808498054047919E-5 0.0 3.808498054047919E-5 0.0 7 0.0 3.808498054047919E-5 0.0 3.808498054047919E-5 0.0 8 0.0 7.616996108095838E-5 0.0 3.808498054047919E-5 0.0 9 0.0 7.616996108095838E-5 0.0 3.808498054047919E-5 0.0 10-11 0.0 7.616996108095838E-5 0.0 7.616996108095838E-5 0.0 12-13 0.0 9.521245135119799E-5 0.0 7.616996108095838E-5 0.0 14-15 0.0 1.3329743189167717E-4 0.0 7.616996108095838E-5 0.0 16-17 0.0 1.5233992216191677E-4 0.0 7.616996108095838E-5 0.0 18-19 0.0 1.7138241243215636E-4 0.0 7.616996108095838E-5 0.0 20-21 0.0 1.9042490270239595E-4 0.0 7.616996108095838E-5 0.0 22-23 0.0 1.9042490270239595E-4 0.0 2.8563735405359394E-4 0.0 24-25 0.0 2.6659486378335435E-4 0.0 4.1893478594527114E-4 0.0 26-27 0.0 3.0467984432383353E-4 0.0 6.474446691881463E-4 0.0 28-29 0.0 3.237223345940731E-4 0.0 0.0016757391437810846 0.0 30-31 0.0 3.427648248643127E-4 0.0 0.004627325135668222 0.0 32-33 0.0 3.427648248643127E-4 0.0 0.010739964512415132 0.0 34-35 0.0 3.427648248643127E-4 0.0 0.02029929462807541 0.0 36-37 0.0 3.427648248643127E-4 0.0 0.03507626707778134 0.0 38-39 0.0 3.427648248643127E-4 0.0 0.052595358126401766 0.0 40-41 5.712747081071879E-5 3.427648248643127E-4 0.0 0.06992402427231979 0.0 42-43 7.616996108095838E-5 3.808498054047919E-4 0.0 0.08658620325877944 0.0 44-45 7.616996108095838E-5 3.808498054047919E-4 0.0 0.1067141154744227 0.0 46-47 7.616996108095838E-5 3.808498054047919E-4 0.0 0.13002212356519596 0.0 48-49 7.616996108095838E-5 3.808498054047919E-4 0.0 0.1545298085429943 0.0 50-51 7.616996108095838E-5 3.808498054047919E-4 0.0 0.17658101227593176 0.0 52-53 7.616996108095838E-5 3.808498054047919E-4 0.0 0.19756583655373583 0.0 54-55 7.616996108095838E-5 3.808498054047919E-4 0.0 0.22081671717369836 0.0 56-57 7.616996108095838E-5 3.808498054047919E-4 0.0 0.24865683794878862 0.0 58-59 7.616996108095838E-5 3.808498054047919E-4 0.0 0.2764969587238789 0.0 60-61 7.616996108095838E-5 3.808498054047919E-4 0.0 0.303765804790862 0.0 62-63 7.616996108095838E-5 3.808498054047919E-4 0.0 0.3318344354491952 0.0 64-65 7.616996108095838E-5 3.808498054047919E-4 0.0 0.36020774595185223 0.0 66-67 7.616996108095838E-5 4.1893478594527114E-4 0.0 0.39191349225180117 0.0 68-69 7.616996108095838E-5 4.1893478594527114E-4 0.0 0.42622805971877287 0.0 70-71 7.616996108095838E-5 4.951047470262295E-4 0.0 0.4608473070300685 0.0 72-73 7.616996108095838E-5 4.951047470262295E-4 0.0 0.4971042085046047 0.0 74-75 7.616996108095838E-5 4.951047470262295E-4 0.0 0.5322185605629265 0.0 76-77 7.616996108095838E-5 4.951047470262295E-4 0.0 0.5698465213369199 0.0 78-79 7.616996108095838E-5 4.951047470262295E-4 0.0 0.6117590424217172 0.0 80-81 7.616996108095838E-5 4.951047470262295E-4 0.0 0.6534240111330015 0.0 82-83 7.616996108095838E-5 4.951047470262295E-4 0.0 0.6946319600777999 0.0 84-85 7.616996108095838E-5 4.951047470262295E-4 0.0 0.7368110760263807 0.0 86-87 7.616996108095838E-5 4.951047470262295E-4 0.0 0.7798661465273924 0.0 88 7.616996108095838E-5 5.331897275667087E-4 0.0 0.8125049748505832 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3800 0.0 36.52078 1 GGTATCA 2370 0.0 36.44845 1 TCAACGC 4240 0.0 31.400335 4 ATCAACG 4345 0.0 30.749798 3 CAACGCA 4725 0.0 28.172926 5 AACGCAG 4855 0.0 27.66377 6 TATCAAC 4960 0.0 27.127806 2 GTACATG 10310 0.0 25.318779 1 TACATGG 10725 0.0 24.21431 2 ACGCAGA 5860 0.0 22.918076 7 ACATGGG 10960 0.0 22.706951 3 CGCAGAG 6390 0.0 20.87023 8 CATGGGG 9365 0.0 20.294918 4 GTATAAC 650 0.0 18.15528 1 GTACCGA 160 0.0012863798 17.610264 6 GCAGAGT 7525 0.0 17.597555 9 GAGTACA 7065 0.0 17.43832 1 AGAGTAC 6245 0.0 16.955692 10-11 CCTAGAC 640 0.0 16.906805 3 TACTCCG 525 0.0 16.127258 5 >>END_MODULE