FastQCFastQC Report
Wed 25 May 2016
SRR2049393_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049393_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2625707
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46280.1762572899413377No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37180.14159995764950165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34350.047.177821
GTATCAA62800.045.083851
TCAACGC77300.036.072644
ATCAACG77850.035.7573933
CAACGCA81800.034.0882075
TATCAAC82100.034.0729982
AACGCAG84900.032.885756
ACGCAGA98750.028.1295537
CGCAGAG102600.027.02828
GTACATG102550.026.5061281
TACGACC2601.8189894E-1225.3196354
TACATGG104750.024.9998662
ACATGGG107850.023.5437893
GCAGAGT117600.023.5407589
CATGGGG90500.021.5106984
CGATACA3700.020.3308162
AGAGTAC106400.020.00796310-11
GAGTACT61350.019.87783612-13
CAGAGTA110350.019.54729310-11
GTGGTAT18700.018.8940831
Produced by FastQC (version 0.11.5)