##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049393_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2625707
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.0436743322846	34.0	33.0	34.0	31.0	34.0
2	33.145827390489494	34.0	33.0	34.0	31.0	34.0
3	33.246948726571546	34.0	34.0	34.0	31.0	34.0
4	36.57042427049172	37.0	37.0	37.0	35.0	37.0
5	36.53462096113542	37.0	37.0	37.0	35.0	37.0
6	36.55758658525114	37.0	37.0	37.0	35.0	37.0
7	36.53248972562437	37.0	37.0	37.0	35.0	37.0
8	36.53149037573499	37.0	37.0	37.0	35.0	37.0
9	38.34883557076247	39.0	39.0	39.0	37.0	39.0
10-11	38.36407146722768	39.0	39.0	39.0	37.0	39.0
12-13	38.35922496302901	39.0	39.0	39.0	37.0	39.0
14-15	39.99975244762649	41.0	40.0	41.0	38.0	41.0
16-17	40.007405624466095	41.0	40.0	41.0	38.0	41.0
18-19	40.011133001511595	41.0	40.0	41.0	38.0	41.0
20-21	40.00291159676232	41.0	40.0	41.0	38.0	41.0
22-23	39.977142918078826	41.0	40.0	41.0	38.0	41.0
24-25	39.953567362999756	41.0	40.0	41.0	38.0	41.0
26-27	39.88653989192244	41.0	40.0	41.0	38.0	41.0
28-29	39.823656638002646	41.0	40.0	41.0	38.0	41.0
30-31	39.74331884707624	41.0	40.0	41.0	37.0	41.0
32-33	39.65187395242501	41.0	40.0	41.0	37.0	41.0
34-35	39.585296836242584	41.0	40.0	41.0	36.0	41.0
36-37	39.50163822543795	41.0	40.0	41.0	36.0	41.0
38-39	39.41724133728554	41.0	40.0	41.0	35.5	41.0
40-41	39.31309414949955	41.0	39.5	41.0	35.0	41.0
42-43	39.19573947131192	41.0	39.0	41.0	35.0	41.0
44-45	39.051210397809044	41.0	39.0	41.0	35.0	41.0
46-47	38.854217359362636	41.0	39.0	41.0	35.0	41.0
48-49	38.646719912008464	41.0	39.0	41.0	35.0	41.0
50-51	38.46775363740127	41.0	38.0	41.0	35.0	41.0
52-53	38.318934862115235	40.0	38.0	41.0	35.0	41.0
54-55	38.202600670981184	40.0	37.0	41.0	35.0	41.0
56-57	38.045271806793366	40.0	37.0	41.0	35.0	41.0
58-59	37.82160957029859	40.0	36.0	41.0	34.5	41.0
60-61	37.56901607833623	39.5	35.5	41.0	34.0	41.0
62-63	37.29501520923698	39.0	35.0	41.0	34.0	41.0
64-65	37.003850391532644	39.0	35.0	41.0	34.0	41.0
66-67	36.69067359762533	37.5	35.0	41.0	34.0	41.0
68-69	36.36339165032504	37.0	35.0	40.0	33.5	41.0
70-71	36.02744536995179	36.5	35.0	39.0	33.0	41.0
72-73	35.69030226906506	36.0	35.0	39.0	33.0	41.0
74-75	35.351453722749724	35.5	35.0	39.0	33.0	41.0
76-77	35.041217470189935	35.0	35.0	37.0	33.0	39.5
78-79	34.73950292245098	35.0	35.0	37.0	33.0	39.0
80-81	34.4661628277641	35.0	35.0	36.5	33.0	39.0
82-83	34.22592162796535	35.0	35.0	36.0	32.5	37.5
84-85	34.006506247650634	35.0	35.0	36.0	32.0	37.0
86-87	33.80479409926545	35.0	35.0	35.5	32.0	37.0
88-89	33.66201807741686	35.0	35.0	35.0	32.0	36.0
90-91	33.54384819022077	35.0	35.0	35.0	32.0	36.0
92-93	33.434296553271174	35.0	35.0	35.0	32.0	36.0
94-95	33.34543877134806	35.0	35.0	35.0	32.0	36.0
96-97	33.32837308199277	35.0	35.0	35.0	32.0	35.5
98-99	33.276750033419574	35.0	35.0	35.0	32.0	35.0
100	33.22316541792363	35.0	35.0	35.0	32.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.041864568541598146
1101	2	-0.0392828943120449
1101	3	-0.02628579923778318
1101	4	-0.02496592866344116
1101	5	-0.003782333215085032
1101	6	-0.035850769925019677
1101	7	0.02099907736935336
1101	8	0.048322044127502295
1101	9	0.08910671235289414
1101	10-11	0.008050495564127402
1101	12-13	0.02087823509224762
1101	14-15	-0.008308967337960382
1101	16-17	-0.08442729898268198
1101	18-19	-0.02771060890331256
1101	20-21	-0.03819813960571139
1101	22-23	-0.04610139330277008
1101	24-25	-0.03710276882652863
1101	26-27	0.01371133148337833
1101	28-29	0.02620008239565408
1101	30-31	-0.05831448215513291
1101	32-33	0.03889436942261426
1101	34-35	-0.011348463011437104
1101	36-37	-0.05256447036207135
1101	38-39	0.010005613539078695
1101	40-41	-0.07030522735084332
1101	42-43	-0.07795870633271562
1101	44-45	-0.041549210657954916
1101	46-47	-0.08707449147615165
1101	48-49	0.014706870249355575
1101	50-51	-0.06901317471636048
1101	52-53	-0.03737527768528537
1101	54-55	-0.011550778849908738
1101	56-57	0.012406423971988545
1101	58-59	-0.0660376463442276
1101	60-61	-0.1171973765131824
1101	62-63	-0.06771655678811328
1101	64-65	-0.12956618337600645
1101	66-67	-0.06411555795188661
1101	68-69	-0.011157656174098918
1101	70-71	-0.049463578163894795
1101	72-73	-0.05501835649957343
1101	74-75	-0.05712512961623872
1101	76-77	-0.032232240488575314
1101	78-79	-0.07440016178853881
1101	80-81	-0.05038067686719927
1101	82-83	-0.04394732566497339
1101	84-85	-0.07698983135213666
1101	86-87	-0.012209846544926961
1101	88-89	-0.07643127043650821
1101	90-91	-0.06580159899807114
1101	92-93	-0.056585039311677576
1101	94-95	-0.003386013456008641
1101	96-97	0.006735680482705675
1101	98-99	-0.0032272654941181145
1101	100	-0.019540561421244718
1102	1	-0.027833333261469306
1102	2	-0.03527468983032378
1102	3	-0.07027406903250721
1102	4	-0.04627341010706232
1102	5	-0.07477527109171689
1102	6	-0.16372213567741767
1102	7	-0.05721194248120298
1102	8	-0.040051822642340085
1102	9	0.07872478886058332
1102	10-11	0.016740105911033254
1102	12-13	0.033994473363861744
1102	14-15	-0.02356177871388354
1102	16-17	-0.048750230662818694
1102	18-19	-0.018673251863582152
1102	20-21	0.05245373917875895
1102	22-23	0.029940384129950814
1102	24-25	0.022308235479414407
1102	26-27	0.002858585531491542
1102	28-29	-0.030570686212932685
1102	30-31	0.020885371623002413
1102	32-33	0.09245867693780241
1102	34-35	-0.0015846087669615372
1102	36-37	-0.04497024288031071
1102	38-39	0.015182866985050225
1102	40-41	-2.2967796189021783E-4
1102	42-43	0.012228200575108872
1102	44-45	-0.008579467566868004
1102	46-47	0.056050582456855125
1102	48-49	0.08129952917702354
1102	50-51	-0.0011892883662838472
1102	52-53	0.04668175567627486
1102	54-55	0.07270799286077079
1102	56-57	0.015816801368750077
1102	58-59	0.03773909711881629
1102	60-61	-0.04456785549656672
1102	62-63	-0.045460042462941885
1102	64-65	0.002514740862359588
1102	66-67	-0.02222038758674927
1102	68-69	-0.01663943886807573
1102	70-71	0.04076014044100873
1102	72-73	0.05247308008330265
1102	74-75	0.049229713790978735
1102	76-77	0.003892675008934532
1102	78-79	-0.002459984639720858
1102	80-81	0.047354336138603514
1102	82-83	-0.009491534506018695
1102	84-85	0.03823441038882436
1102	86-87	0.03093051577270245
1102	88-89	0.030470751943461494
1102	90-91	0.036507673065571566
1102	92-93	0.009396468575715744
1102	94-95	-0.006309255088936538
1102	96-97	0.021430679344618397
1102	98-99	0.014684144722707515
1102	100	0.06541981048792422
1103	1	-0.15196731893392013
1103	2	-0.0990211997306858
1103	3	-0.08274877381489887
1103	4	-0.05555814246871904
1103	5	-0.0840617111473847
1103	6	-0.028834800790079385
1103	7	-0.04377580596813857
1103	8	-0.05121672611493011
1103	9	-0.06926568220681162
1103	10-11	-0.04817361284884214
1103	12-13	-0.09164569843016324
1103	14-15	-0.16756990733739485
1103	16-17	-0.12610108493674943
1103	18-19	-0.03345590510777896
1103	20-21	-0.06168169803360257
1103	22-23	-0.08263507459935937
1103	24-25	-0.09583515958705391
1103	26-27	-0.12193713157191866
1103	28-29	-0.12728508292732954
1103	30-31	-0.13742725592039307
1103	32-33	-0.12450555541105501
1103	34-35	-0.06380870945260142
1103	36-37	0.001965727132585471
1103	38-39	-0.0629082726445489
1103	40-41	-0.07393545935997281
1103	42-43	-0.04732250512944347
1103	44-45	-0.1480553762735468
1103	46-47	-0.15825135492200815
1103	48-49	-0.24922464750099493
1103	50-51	-0.14965279067594395
1103	52-53	-0.12470156186088843
1103	54-55	-0.13164150379641626
1103	56-57	-0.11305347365537699
1103	58-59	-0.14079604295477566
1103	60-61	-0.2065955713704355
1103	62-63	-0.2262997156103097
1103	64-65	-0.14251031954731985
1103	66-67	-0.11382707150112736
1103	68-69	-0.13690891298369934
1103	70-71	-0.18775578807492366
1103	72-73	-0.2376940177761
1103	74-75	-0.21206454750327453
1103	76-77	-0.19499220079901392
1103	78-79	-0.31351644954234104
1103	80-81	-0.24434029401756874
1103	82-83	-0.2942563423477509
1103	84-85	-0.2308725510258256
1103	86-87	-0.1976016358363708
1103	88-89	-0.23591072982264194
1103	90-91	-0.20105988757891424
1103	92-93	-0.16619204547433242
1103	94-95	-0.17331575286466006
1103	96-97	-0.20304142974288197
1103	98-99	-0.1716739416353832
1103	100	-0.22236249267898955
1104	1	-0.11473212447180714
1104	2	-0.08347896585101466
1104	3	-0.07406721608017364
1104	4	-0.07437360538389015
1104	5	-0.0058716511449787845
1104	6	-0.005032918851895829
1104	7	-0.007807908098030225
1104	8	-0.05657990898961884
1104	9	0.0072221607515530195
1104	10-11	-0.02438262547595116
1104	12-13	-0.058192446500392236
1104	14-15	-0.0879668065403294
1104	16-17	-0.049135566235918304
1104	18-19	-0.0582959842540518
1104	20-21	-0.01120169054065201
1104	22-23	-0.08677575400482596
1104	24-25	-0.1599880032841412
1104	26-27	-0.11553998391018894
1104	28-29	-0.06653373413703889
1104	30-31	-0.031434970683434926
1104	32-33	-0.07305158161835834
1104	34-35	-0.07789871345381272
1104	36-37	-0.08181755566642579
1104	38-39	-0.10691432187152827
1104	40-41	-0.10833497090723654
1104	42-43	-0.13988422107833287
1104	44-45	-0.21210950266853246
1104	46-47	-0.15228932365773318
1104	48-49	-0.182247524912448
1104	50-51	-0.15673802027106376
1104	52-53	-0.20924950206304516
1104	54-55	-0.20641886081406824
1104	56-57	-0.25832015733010394
1104	58-59	-0.19636831918609232
1104	60-61	-0.18077133676263202
1104	62-63	-0.11755753236293742
1104	64-65	-0.11300685699581692
1104	66-67	-0.14254483211147573
1104	68-69	-0.18371251414698264
1104	70-71	-0.13337521269295394
1104	72-73	-0.13874287441498723
1104	74-75	-0.19043295702606855
1104	76-77	-0.0905291911792716
1104	78-79	-0.09059867333473903
1104	80-81	-0.08724779484739287
1104	82-83	-0.13531423598936243
1104	84-85	-0.13694897522572091
1104	86-87	-0.07711597335862308
1104	88-89	-0.08924056748968923
1104	90-91	-0.13818782151866316
1104	92-93	-0.13594849707522627
1104	94-95	-0.06170626111180866
1104	96-97	-0.12088237771114052
1104	98-99	-0.12940827962006551
1104	100	-0.12252681250737396
1105	1	-0.08156276990384725
1105	2	-0.10590403336446741
1105	3	-0.09207055299334854
1105	4	-0.07587947893200919
1105	5	-0.041403778647804756
1105	6	-0.06878508646860126
1105	7	-0.08339778087208316
1105	8	-0.032730674393604886
1105	9	-0.05138627176309285
1105	10-11	-0.04855717704288054
1105	12-13	-0.09522765610237371
1105	14-15	-0.10721746847209346
1105	16-17	-0.09923015486121756
1105	18-19	-0.07289182959721785
1105	20-21	-0.11201343635433147
1105	22-23	-0.09135686125191711
1105	24-25	-0.10489087406305941
1105	26-27	-0.0725763402695847
1105	28-29	-0.05169427828851525
1105	30-31	-0.07107878934034773
1105	32-33	-0.045733896242573735
1105	34-35	-0.08685202194325115
1105	36-37	-0.125401429088555
1105	38-39	-0.07392135670013289
1105	40-41	-0.044371784837920814
1105	42-43	-0.055805853789287596
1105	44-45	-0.04033195700854009
1105	46-47	-0.02513486260165365
1105	48-49	-6.89722869182674E-4
1105	50-51	-0.0012704205060032336
1105	52-53	0.002407324746030781
1105	54-55	-0.039147279612890884
1105	56-57	-0.016688185733478633
1105	58-59	-0.0029731514860245056
1105	60-61	-0.10678153007801683
1105	62-63	-0.10688435181238987
1105	64-65	-0.045766396678338594
1105	66-67	-0.021128684786340557
1105	68-69	-0.029986439033628187
1105	70-71	-0.014988089269792226
1105	72-73	-0.03775149508838638
1105	74-75	-0.048436475006205626
1105	76-77	-0.0823983416319578
1105	78-79	-0.020956586130623123
1105	80-81	-0.024990523594375702
1105	82-83	0.003869672589928541
1105	84-85	-0.03193960689667108
1105	86-87	-0.001117266478502188
1105	88-89	-0.039589140248246224
1105	90-91	-0.007250494451746192
1105	92-93	-0.024294386270547363
1105	94-95	-0.04727605431448012
1105	96-97	-0.03608038386213508
1105	98-99	-0.030818187679550135
1105	100	0.04526099814034268
1106	1	-0.06596891250786996
1106	2	-0.06417904794010809
1106	3	-0.05862958808162233
1106	4	-0.021007194939173246
1106	5	-0.007065103089225033
1106	6	-0.047081679254873166
1106	7	-0.057483746739187325
1106	8	-0.06069145997078351
1106	9	-0.03673848822905512
1106	10-11	-0.009992234002282885
1106	12-13	-0.0444551456385085
1106	14-15	-0.05656889325169345
1106	16-17	-0.08154896704911607
1106	18-19	-0.05916514547798357
1106	20-21	-0.00932532092610927
1106	22-23	-0.06523769789373546
1106	24-25	-0.06297924699116209
1106	26-27	-0.033666223701267484
1106	28-29	-0.13999559856104327
1106	30-31	-0.10794658715846595
1106	32-33	-0.054769997184315855
1106	34-35	-0.10748837518095655
1106	36-37	-0.20928450658335862
1106	38-39	-0.11403060711282365
1106	40-41	-0.15324646363878003
1106	42-43	-0.03282496576426297
1106	44-45	-0.013812121351215012
1106	46-47	-0.182215990489496
1106	48-49	-0.14737766070133063
1106	50-51	-0.11479322952802562
1106	52-53	-0.10932453171454881
1106	54-55	-0.12884230377324002
1106	56-57	-0.13718441538480164
1106	58-59	-0.09443166712741657
1106	60-61	-0.10056685693803757
1106	62-63	-0.0822101025720201
1106	64-65	-0.06623041541933361
1106	66-67	-0.09205579032143163
1106	68-69	0.004938973861023044
1106	70-71	0.03357899371694373
1106	72-73	-0.07442764195427287
1106	74-75	-0.04172270934321176
1106	76-77	-0.08888740934701644
1106	78-79	-0.05328044005756993
1106	80-81	-0.07883305311018063
1106	82-83	-0.05655324534663464
1106	84-85	-0.04013536994747113
1106	86-87	-0.011734469757016086
1106	88-89	-0.06377265781948438
1106	90-91	-0.04196292180152028
1106	92-93	-0.10190898564761852
1106	94-95	-0.05695085962371138
1106	96-97	-0.1103573808250573
1106	98-99	-0.12171818386671163
1106	100	-0.12202289414850753
1107	1	-0.06754686738885596
1107	2	-0.0801907493805345
1107	3	-0.08031150078512184
1107	4	-0.027675153743267344
1107	5	0.013502811521540536
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2216	4	0.030301588433253812
2216	5	0.018284142198915276
2216	6	0.06565587231330738
2216	7	0.051285083934899944
2216	8	0.0520900752799065
2216	9	0.0691359191561034
2216	10-11	0.04609392486023012
2216	12-13	0.05960858308201722
2216	14-15	0.06956519707205189
2216	16-17	0.06844317323579219
2216	18-19	0.058638261286084514
2216	20-21	0.06708810930445708
2216	22-23	0.06227988149834829
2216	24-25	0.056238370854636344
2216	26-27	0.057414049335513084
2216	28-29	0.06576050347086237
2216	30-31	0.03861093443526897
2216	32-33	0.062232384283753106
2216	34-35	0.05860898697576289
2216	36-37	0.0654210357190621
2216	38-39	0.023608160381442644
2216	40-41	0.07464958535668131
2216	42-43	0.09629346094095581
2216	44-45	0.07732749637477099
2216	46-47	0.06806127568142273
2216	48-49	0.061492040583409846
2216	50-51	0.044903764374168986
2216	52-53	0.065804027110687
2216	54-55	0.03789579157458434
2216	56-57	0.04080408126932156
2216	58-59	0.03965420192409397
2216	60-61	0.02136313066563389
2216	62-63	0.08813478275038733
2216	64-65	0.05906913701213057
2216	66-67	0.046077371173431914
2216	68-69	0.07032099953745075
2216	70-71	0.0754405531560991
2216	72-73	0.06818845013393116
2216	74-75	0.0448898660985364
2216	76-77	0.06836147531588921
2216	78-79	0.031457222109850136
2216	80-81	-0.0060446412488133205
2216	82-83	-0.025982205042936357
2216	84-85	-0.014056041612604986
2216	86-87	-0.0037023529642112862
2216	88-89	0.022371176502844037
2216	90-91	-0.06568185224534062
2216	92-93	-0.08187170448435666
2216	94-95	-0.032461779507357846
2216	96-97	-0.0071140578706447855
2216	98-99	-0.007382933991777918
2216	100	-0.009884012214740778
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	2.0
4	7.0
5	25.0
6	70.0
7	157.0
8	329.0
9	631.0
10	944.0
11	1162.0
12	1730.0
13	2305.0
14	2653.0
15	3797.0
16	5176.0
17	8033.0
18	9708.0
19	5705.0
20	4058.0
21	4084.0
22	4169.0
23	4475.0
24	4947.0
25	5579.0
26	6935.0
27	8585.0
28	10438.0
29	13727.0
30	17621.0
31	23161.0
32	30423.0
33	39529.0
34	58002.0
35	102195.0
36	231619.0
37	603789.0
38	977009.0
39	430802.0
40	2125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.03847671951572	18.52872622498628	14.30390638181567	25.12889067368233
2	14.771869062313502	18.87167913251555	39.352601032788506	27.00385077238245
3	18.022650661326644	23.563329800316637	29.032942365618098	29.381077172738618
4	12.760068050243229	15.784091675118358	35.69415780207007	35.76168247256834
5	14.766934914885413	36.39568567871472	33.655386120478546	15.18199328592131
6	33.98403146934782	35.035777415608756	16.4343397679486	14.545851347094827
7	29.860071972996227	30.85233805599787	20.248984368781436	19.038605602224468
8	27.172414896254608	34.613458394253435	18.809829124117808	19.40429758537415
9	26.339833756059512	15.852364898707597	19.526483201348054	38.28131814388484
10-11	25.894959980256722	25.52846779378026	26.72258563798509	21.85398658797793
12-13	26.23072566741072	22.73218222749149	27.301065960520347	23.73602614457744
14-15	23.367458745396956	24.421917601621203	24.668232213266748	27.542391439715093
16-17	23.434621608580088	27.72175265557048	25.15693868356218	23.68668705228725
18-19	23.52855821308318	26.519447904888093	27.215679434148594	22.736314447880133
20-21	24.250344764286343	25.585756521957702	27.21156625625022	22.95233245750573
22-23	24.081952022826613	25.30878350097707	27.249422726907458	23.359841749288858
24-25	23.869266448998307	25.46790254967519	27.098530034006078	23.56430096732042
26-27	23.61579635804687	25.729513180547787	27.12997435166497	23.524716109740368
28-29	23.551694838761524	25.662116907941368	27.285222608615506	23.500965644681603
30-31	23.682151913293385	25.705435768687906	27.102577539854405	23.5098347781643
32-33	23.577097675151965	25.676120087664295	27.236961897948298	23.509820339235446
34-35	23.770190867778858	25.547474722854012	27.196639971481808	23.485694437885325
36-37	23.88462644416896	25.634748795057977	26.965145707054837	23.51547905371823
38-39	23.75119301246979	25.72371011834531	26.94412474206937	23.580972127115526
40-41	23.717058107525503	25.656781242525756	27.06936554436863	23.556795105580107
42-43	23.80724318642312	25.680443625173293	26.928234639935404	23.58407854846818
44-45	23.726632021343246	25.863889200980328	26.79182908645652	23.617649691219906
46-47	23.90622042442955	25.780528463195758	26.625659017499785	23.68759209487491
48-49	23.972224486236023	25.91842999487359	26.32237237500295	23.78697314388743
50-51	23.844149631649046	26.043362694006483	26.338112012358216	23.774375661986248
52-53	23.90373227667506	26.070940526409604	26.261065890321433	23.764261306593905
54-55	23.93787433188685	26.122072089570768	26.268662798713322	23.671390779829057
56-57	23.85464079330881	25.987284725636705	26.363018683676227	23.795055797378257
58-59	23.80371303890137	25.816707075462	26.46004861242824	23.919531273208396
60-61	23.99497501790964	25.959113083424835	26.28895132348522	23.756960575180305
62-63	23.796733957484278	26.01329946924381	26.401878280091722	23.78808829318019
64-65	23.843110728721886	25.904337185028776	26.41269775978568	23.83985432646366
66-67	23.820008398008472	25.90726580077677	26.37903963726697	23.893686163947788
68-69	23.88539483411418	25.969665265193743	26.330501060531216	23.814438840160864
70-71	23.902551150800164	25.85275455804772	26.2818313012922	23.962862989859918
72-73	23.84380033769997	25.988844513479908	26.282080212026987	23.88527493679313
74-75	23.90707185272628	26.020403013892036	26.145667639698715	23.926857493682974
76-77	23.91072563320782	25.977616662639107	26.13586267978109	23.975795024371983
78-79	23.92218926387185	25.978970596272354	26.238215305644637	23.860624834211162
80-81	23.769997947219547	25.971214610007898	26.30550552670195	23.953281916070605
82-83	23.8410183648205	25.957711459365402	26.26528664075992	23.935983535054184
84-85	23.907906550723094	25.97489624205332	26.072260661670544	24.044936545553046
86-87	23.900969212456086	26.09599348894585	26.10187765989759	23.901159638700477
88-89	23.723808586188756	26.048242112036306	26.190813969718857	24.037135332056085
90-91	23.792296565545158	25.988541661112563	26.12195441496051	24.097207358381773
92-93	23.87505819871063	26.143422569769427	26.074222041529836	23.907297189990107
94-95	23.81195400232624	25.945024903854137	26.194920360224216	24.04810073359541
96-97	23.836418153282143	26.062085373577474	26.103997894662275	23.9974985784781
98-99	23.66652251465847	26.16798831397564	26.17179682145774	23.993692349908148
100	23.887057841381605	25.898256387444405	26.029269169569385	24.1854166016046
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	195.0
1	155.0
2	187.0
3	243.5
4	147.0
5	110.5
6	316.0
7	905.0
8	1571.5
9	1726.0
10	1251.0
11	806.5
12	790.0
13	929.5
14	1038.5
15	1135.0
16	1308.0
17	1477.0
18	1655.0
19	1841.5
20	2051.5
21	2301.0
22	2753.5
23	3550.5
24	4659.0
25	6072.0
26	7982.5
27	10596.5
28	13928.0
29	17944.5
30	22077.0
31	26420.0
32	30960.5
33	35481.0
34	40075.5
35	44781.5
36	50372.0
37	56758.5
38	63136.5
39	68188.5
40	73061.5
41	78542.0
42	81662.0
43	83910.5
44	87552.5
45	89834.0
46	91134.0
47	91022.5
48	90710.5
49	92271.0
50	92985.5
51	93337.0
52	92292.0
53	92223.0
54	92857.0
55	89477.0
56	84791.5
57	78821.0
58	72771.0
59	69272.0
60	64376.0
61	56534.0
62	49466.0
63	42678.0
64	35581.0
65	30159.0
66	24493.5
67	19151.0
68	14813.0
69	11106.0
70	8460.5
71	6269.5
72	4623.5
73	3339.0
74	2574.5
75	2002.0
76	1332.5
77	912.0
78	635.5
79	402.5
80	235.5
81	115.0
82	47.5
83	20.0
84	12.0
85	9.5
86	11.0
87	12.0
88	10.0
89	7.0
90	6.0
91	3.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.23022370736719674
2	0.0
3	0.0
4	0.0
5	0.029515859918871373
6	0.006398276730800504
7	0.0
8	0.0
9	0.010968474395658007
10-11	1.1425494162143758E-4
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.9042490270239596E-5
28-29	0.0
30-31	9.521245135119798E-4
32-33	3.237223345940731E-4
34-35	5.712747081071879E-4
36-37	0.00236126879350971
38-39	3.808498054047919E-5
40-41	8.759545524310214E-4
42-43	0.002551693696212106
44-45	0.001980418988104918
46-47	6.855296497286254E-4
48-49	0.003237223345940731
50-51	0.0032753083264812108
52-53	0.0027040336183740228
54-55	0.0011044644356738966
56-57	0.0017519091048620427
58-59	0.001561484202159647
60-61	9.90209494052459E-4
62-63	0.004322645291344389
64-65	0.004189347859452712
66-67	0.0032181808556704917
68-69	0.00517955735350517
70-71	0.00708380638052913
72-73	1.3329743189167717E-4
74-75	0.002494566225401387
76-77	0.0018471215562132408
78-79	9.521245135119798E-5
80-81	0.0
82-83	2.8563735405359394E-4
84-85	1.7138241243215636E-4
86-87	7.04572139998865E-4
88-89	5.141472372964691E-4
90-91	7.99784591350063E-4
92-93	1.7138241243215636E-4
94-95	3.427648248643127E-4
96-97	0.0
98-99	2.4755237351311476E-4
100	3.427648248643127E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2625707.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.07526317773574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.16620415538745	48.35096753572646
2	11.949770578915185	14.596707660416172
3	3.6131744830248564	6.620267473734671
4	1.657126022867383	4.048376318611998
5	0.9326702719894386	2.848154115990267
6	0.5985289214382957	2.1933186837798138
7	0.39263288420273756	1.6786109714440944
8	0.2898729670035054	1.416325419828016
9	0.20355288281041925	1.1188841299409842
>10	1.1174336921652797	12.156631364794151
>50	0.05805558178916361	2.392786337220236
>100	0.020478590560401706	2.0394554215247784
>500	2.4948392292297296E-4	0.08826203853436268
>1k	2.494839229101224E-4	0.4512525284541052
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4628	0.1762572899413377	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3718	0.14159995764950165	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	1.9042490270239596E-5	0.0	0.0	0.0	0.0
18-19	3.808498054047919E-5	0.0	0.0	0.0	0.0
20-21	3.808498054047919E-5	1.9042490270239596E-5	0.0	0.0	0.0
22-23	3.808498054047919E-5	3.808498054047919E-5	0.0	1.9042490270239598E-4	0.0
24-25	3.808498054047919E-5	3.808498054047919E-5	0.0	3.0467984432383353E-4	0.0
26-27	3.808498054047919E-5	3.808498054047919E-5	0.0	5.712747081071879E-4	0.0
28-29	3.808498054047919E-5	3.808498054047919E-5	0.0	0.0016757391437810846	0.0
30-31	3.808498054047919E-5	3.808498054047919E-5	0.0	0.004665410116208701	0.0
32-33	3.808498054047919E-5	3.808498054047919E-5	0.0	0.010739964512415132	0.0
34-35	3.808498054047919E-5	7.616996108095838E-5	0.0	0.020584931982129004	0.0
36-37	3.808498054047919E-5	7.616996108095838E-5	0.0	0.03555232933453733	0.0
38-39	3.808498054047919E-5	1.1425494162143758E-4	0.0	0.05316663283450895	0.0
40-41	3.808498054047919E-5	1.5233992216191677E-4	0.0	0.07068572388312938	0.0
42-43	3.808498054047919E-5	1.5233992216191677E-4	0.0	0.08755737026256166	0.0
44-45	3.808498054047919E-5	1.7138241243215636E-4	0.0	0.10793283485171803	0.0
46-47	3.808498054047919E-5	1.9042490270239595E-4	0.0	0.1312408429424913	0.0
48-49	3.808498054047919E-5	1.9042490270239595E-4	0.0	0.15597703780353253	0.0
50-51	3.808498054047919E-5	2.6659486378335435E-4	0.0	0.17808536900728072	0.0
52-53	3.808498054047919E-5	2.6659486378335435E-4	0.0	0.19926061818778712	0.0
54-55	3.808498054047919E-5	2.6659486378335435E-4	0.0	0.22258766876883063	0.0
56-57	3.808498054047919E-5	2.6659486378335435E-4	0.0	0.2508467243298662	0.0
58-59	3.808498054047919E-5	2.6659486378335435E-4	0.0	0.2788391850271184	0.0
60-61	3.808498054047919E-5	2.6659486378335435E-4	0.0	0.3063174984870741	0.0
62-63	3.808498054047919E-5	2.6659486378335435E-4	0.0	0.33482410642162286	0.0
64-65	3.808498054047919E-5	2.6659486378335435E-4	0.0	0.36357826672968463	0.0
66-67	3.808498054047919E-5	2.6659486378335435E-4	0.0	0.39543635295179547	0.0
68-69	3.808498054047919E-5	2.6659486378335435E-4	0.0	0.43001751528255056	0.0
70-71	3.808498054047919E-5	2.6659486378335435E-4	0.0	0.4646558050841164	0.0
72-73	3.808498054047919E-5	3.427648248643127E-4	0.0	0.5011793014224359	0.0
74-75	3.808498054047919E-5	3.9989229567503155E-4	0.0	0.53646503589319	0.0
76-77	3.808498054047919E-5	4.1893478594527114E-4	0.0	0.5746071439044799	0.0
78-79	3.808498054047919E-5	4.1893478594527114E-4	0.0	0.6167481748725201	0.0
80-81	3.808498054047919E-5	4.1893478594527114E-4	0.0	0.6590034607821817	0.0
82-83	3.808498054047919E-5	4.1893478594527114E-4	0.0	0.700668429493466	0.0
84-85	3.808498054047919E-5	4.1893478594527114E-4	0.0	0.7435521175820455	0.0
86-87	3.808498054047919E-5	4.1893478594527114E-4	0.0	0.7873117602230562	0.0
88	3.808498054047919E-5	4.1893478594527114E-4	0.0	0.8201981409197598	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3435	0.0	47.17782	1
GTATCAA	6280	0.0	45.08385	1
TCAACGC	7730	0.0	36.07264	4
ATCAACG	7785	0.0	35.757393	3
CAACGCA	8180	0.0	34.088207	5
TATCAAC	8210	0.0	34.072998	2
AACGCAG	8490	0.0	32.88575	6
ACGCAGA	9875	0.0	28.129553	7
CGCAGAG	10260	0.0	27.0282	8
GTACATG	10255	0.0	26.506128	1
TACGACC	260	1.8189894E-12	25.319635	4
TACATGG	10475	0.0	24.999866	2
ACATGGG	10785	0.0	23.543789	3
GCAGAGT	11760	0.0	23.540758	9
CATGGGG	9050	0.0	21.510698	4
CGATACA	370	0.0	20.330816	2
AGAGTAC	10640	0.0	20.007963	10-11
GAGTACT	6135	0.0	19.877836	12-13
CAGAGTA	11035	0.0	19.547293	10-11
GTGGTAT	1870	0.0	18.894083	1
>>END_MODULE