FastQCFastQC Report
Wed 25 May 2016
SRR2049392_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049392_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2704906
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24650.037.944491
GTATCAA38750.037.5473631
TCAACGC43100.033.2875824
ATCAACG44350.032.349983
CAACGCA48050.029.8533925
TATCAAC49000.029.1845742
AACGCAG49450.028.9662956
GTACATG103750.026.4538231
TACATGG106650.025.2296372
ACGCAGA57650.024.6787017
ACATGGG110900.023.6684843
CGCAGAG63250.022.2701748
GTATAGA7950.020.797031
CATGGGG96300.020.4666844
GCAGAGT73850.019.0736439
GAGTACA71200.018.9752041
AGAGTAC62100.017.42649510-11
AGTACAT74650.017.2031782
GTCGTAC3401.8757419E-816.6725771
CAGAGTA67700.016.33175810-11
Produced by FastQC (version 0.11.5)