##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049392_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2704906 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.476655750698914 33.0 31.0 34.0 30.0 34.0 2 31.715577916570854 34.0 31.0 34.0 30.0 34.0 3 31.95017793594306 34.0 31.0 34.0 30.0 34.0 4 35.41543033288403 37.0 35.0 37.0 35.0 37.0 5 35.49071908598672 37.0 37.0 37.0 35.0 37.0 6 35.535365369443525 37.0 37.0 37.0 35.0 37.0 7 35.45847951832707 37.0 37.0 37.0 35.0 37.0 8 35.47486530030988 37.0 37.0 37.0 35.0 37.0 9 37.20070974739973 39.0 39.0 39.0 35.0 39.0 10-11 37.20506941830881 39.0 39.0 39.0 35.0 39.0 12-13 37.162606574868036 39.0 39.0 39.0 35.0 39.0 14-15 38.68660075433306 41.0 40.0 41.0 36.0 41.0 16-17 38.66993769838952 41.0 40.0 41.0 35.5 41.0 18-19 38.62691346760294 41.0 40.0 41.0 35.0 41.0 20-21 38.651081405416676 41.0 40.0 41.0 35.0 41.0 22-23 38.60873039580673 41.0 39.5 41.0 35.0 41.0 24-25 38.616799068063735 41.0 39.0 41.0 35.0 41.0 26-27 38.60943873835173 41.0 39.0 41.0 35.0 41.0 28-29 38.57750897813085 41.0 39.0 41.0 35.0 41.0 30-31 38.49657326354409 41.0 39.0 41.0 35.0 41.0 32-33 38.47625222466141 41.0 39.0 41.0 35.0 41.0 34-35 38.42017985098188 41.0 39.0 41.0 34.0 41.0 36-37 38.29408119912485 41.0 39.0 41.0 34.0 41.0 38-39 38.191727365017485 41.0 39.0 41.0 34.0 41.0 40-41 38.08547487417307 41.0 38.0 41.0 33.0 41.0 42-43 37.95600623459743 40.5 38.0 41.0 33.0 41.0 44-45 37.77650092091925 40.0 38.0 41.0 33.0 41.0 46-47 37.63296487937104 40.0 38.0 41.0 33.0 41.0 48-49 37.491641853728005 40.0 37.0 41.0 33.0 41.0 50-51 37.307329164118826 40.0 37.0 41.0 32.5 41.0 52-53 37.12045982374249 40.0 36.5 41.0 32.0 41.0 54-55 36.92690984455652 40.0 36.0 41.0 32.0 41.0 56-57 36.65694038905603 39.5 35.0 41.0 31.0 41.0 58-59 36.423756500225885 39.0 35.0 41.0 31.0 41.0 60-61 36.189194374961644 39.0 35.0 41.0 31.0 41.0 62-63 35.858625031701656 38.0 35.0 40.5 30.5 41.0 64-65 35.63373736462561 37.5 35.0 40.0 30.5 41.0 66-67 35.402339674650435 37.0 35.0 40.0 30.0 41.0 68-69 35.15577010809248 36.5 35.0 39.5 30.0 41.0 70-71 34.92575730912645 36.0 35.0 39.0 30.0 41.0 72-73 34.602124066418575 35.5 35.0 39.0 30.0 41.0 74-75 34.24946929763918 35.0 35.0 38.0 30.0 40.5 76-77 33.92195163159089 35.0 34.0 37.0 30.0 39.0 78-79 33.623294118168985 35.0 34.0 37.0 29.0 39.0 80-81 33.325175440477416 35.0 34.0 36.0 29.0 39.0 82-83 33.05660880636887 35.0 34.0 36.0 29.0 37.0 84-85 32.81953901540386 35.0 34.0 35.5 29.0 37.0 86-87 32.59056266650301 35.0 34.0 35.0 29.0 36.5 88-89 32.303880985143294 35.0 34.0 35.0 27.5 36.0 90-91 32.104363515774665 35.0 34.0 35.0 27.0 36.0 92-93 31.946624762561065 35.0 34.0 35.0 27.0 36.0 94-95 31.812830094650238 35.0 34.0 35.0 26.5 35.5 96-97 31.66405468434023 35.0 33.5 35.0 26.0 35.0 98-99 31.508206015292213 35.0 33.0 35.0 25.0 35.0 100 31.36818617726457 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.17205327290784567 1101 2 -0.2772333020198623 1101 3 -0.13553747481598322 1101 4 0.02163452301184776 1101 5 0.02423078525644229 1101 6 -0.03153380074432022 1101 7 0.00951654059728213 1101 8 -0.11759811409219623 1101 9 0.04397644311065818 1101 10-11 -0.036045008141179835 1101 12-13 0.06308036555129348 1101 14-15 0.05218542535242676 1101 16-17 0.05424931246776765 1101 18-19 -0.08949380851135658 1101 20-21 -0.04622712199146406 1101 22-23 -0.07332046805890968 1101 24-25 0.007008186125133875 1101 26-27 -0.031074846799548084 1101 28-29 -0.015322253865875268 1101 30-31 -0.009576713345545329 1101 32-33 -0.04851218333502061 1101 34-35 0.007728585444858993 1101 36-37 -0.04144864834783846 1101 38-39 0.03522244923723861 1101 40-41 0.07527063365363063 1101 42-43 -0.034042728273362854 1101 44-45 -0.09841522669858449 1101 46-47 -0.0754052458850154 1101 48-49 -0.07670660784623351 1101 50-51 -0.058434842860044967 1101 52-53 -0.11221677334914659 1101 54-55 -0.11557333120725133 1101 56-57 -0.07191158513245455 1101 58-59 -0.11176594298871123 1101 60-61 -0.16183701048577603 1101 62-63 -0.22057404271485836 1101 64-65 -0.16029792440156854 1101 66-67 -0.0760696188847021 1101 68-69 0.047213571552099154 1101 70-71 -0.15526304589708673 1101 72-73 -0.1650415918595698 1101 74-75 -0.07124247522514793 1101 76-77 -0.0880464803810952 1101 78-79 -0.10503670157876144 1101 80-81 -0.14336193061665625 1101 82-83 -0.0865417193320468 1101 84-85 -0.13544998620577076 1101 86-87 -0.2351586382072881 1101 88-89 -0.21290585178554977 1101 90-91 -0.12899960660965348 1101 92-93 -0.03182459423707229 1101 94-95 -0.0539958802089302 1101 96-97 0.025786941620602022 1101 98-99 -0.09509176423383181 1101 100 -0.05026600259469305 1102 1 -0.5060347768533973 1102 2 -0.5530274079807391 1102 3 -0.47139438629218944 1102 4 -0.38132243373200225 1102 5 -0.18233857353264682 1102 6 -0.2823300726023916 1102 7 -0.22801697184644354 1102 8 -0.23162886684485073 1102 9 -0.25070625177173866 1102 10-11 -0.14178304763236582 1102 12-13 -0.20940821179781466 1102 14-15 -0.23533129450909485 1102 16-17 -0.24527347473609495 1102 18-19 -0.27036375546535396 1102 20-21 -0.2565509122219254 1102 22-23 -0.26988821677269925 1102 24-25 -0.2555839706383338 1102 26-27 -0.2390989337775835 1102 28-29 -0.22457465219410722 1102 30-31 -0.2505551815758764 1102 32-33 -0.28668068494768306 1102 34-35 -0.30255210696928714 1102 36-37 -0.23620356862946323 1102 38-39 -0.272592815377223 1102 40-41 -0.21725866413498807 1102 42-43 -0.2854870004069099 1102 44-45 -0.40847598817300224 1102 46-47 -0.36276862337015814 1102 48-49 -0.3665204366005739 1102 50-51 -0.45170033342750315 1102 52-53 -0.5295365372907312 1102 54-55 -0.4628083963830605 1102 56-57 -0.4668832209202094 1102 58-59 -0.41573369368364865 1102 60-61 -0.46956996314531096 1102 62-63 -0.6840118506476571 1102 64-65 -0.4774395187314582 1102 66-67 -0.49239523208517966 1102 68-69 -0.5202918227504654 1102 70-71 -0.3381667742434189 1102 72-73 -0.26689811559693766 1102 74-75 -0.278286248046669 1102 76-77 -0.31916135060572515 1102 78-79 -0.3037001032307529 1102 80-81 -0.29399732859378247 1102 82-83 -0.34950247170262116 1102 84-85 -0.31591179322283836 1102 86-87 -0.39377133821079724 1102 88-89 -0.38558316486547284 1102 90-91 -0.41116954785605486 1102 92-93 -0.336094823677513 1102 94-95 -0.4395017984149021 1102 96-97 -0.5344287345693566 1102 98-99 -0.5074475355282964 1102 100 -0.40509452249995803 1103 1 -0.37436328354443305 1103 2 -0.4730096302644249 1103 3 -0.32174803915577144 1103 4 -0.2237738379142229 1103 5 -0.15710474163194021 1103 6 -0.010233980742150095 1103 7 -0.11641762656424248 1103 8 -0.13173976629819606 1103 9 -0.11715411577605295 1103 10-11 -0.04185249903922994 1103 12-13 -0.15913292224728792 1103 14-15 -0.15508307487363027 1103 16-17 -0.20510009974728405 1103 18-19 -0.20268040163847445 1103 20-21 -0.16428836746552378 1103 22-23 -0.12478532843157808 1103 24-25 -0.14874481249080418 1103 26-27 -0.1643741368979761 1103 28-29 -0.16602934417826276 1103 30-31 -0.15918290551729086 1103 32-33 -0.18153997478400186 1103 34-35 -0.1764971504705315 1103 36-37 -0.10956311525928442 1103 38-39 -0.11590663855040617 1103 40-41 -0.13665243171631403 1103 42-43 -0.20099356352644548 1103 44-45 -0.35052128600928967 1103 46-47 -0.44899139259903365 1103 48-49 -0.39966927042771516 1103 50-51 -0.4228194959849034 1103 52-53 -0.4725172395566233 1103 54-55 -0.5074661119618824 1103 56-57 -0.36359968286272704 1103 58-59 -0.3851210270223575 1103 60-61 -0.495532881164614 1103 62-63 -0.5067871872545808 1103 64-65 -0.5239787220915559 1103 66-67 -0.4074824362396683 1103 68-69 -0.3932566718108177 1103 70-71 -0.38816610200912294 1103 72-73 -0.3647690919385127 1103 74-75 -0.41639793804755243 1103 76-77 -0.36226777989301695 1103 78-79 -0.3914394330477222 1103 80-81 -0.35013765116637074 1103 82-83 -0.40733813077457626 1103 84-85 -0.4672169841393057 1103 86-87 -0.42278838952992714 1103 88-89 -0.3973852560135356 1103 90-91 -0.3726726868061192 1103 92-93 -0.3585298560734458 1103 94-95 -0.2953845592158828 1103 96-97 -0.29649639668058114 1103 98-99 -0.29882180908309763 1103 100 -0.26385202607528413 1104 1 -0.4819907618087349 1104 2 -0.3486251872015913 1104 3 -0.28258726900645925 1104 4 -0.12545446871821753 1104 5 -0.10896350153759471 1104 6 -0.11106590779310466 1104 7 -0.15750080940068756 1104 8 -0.08455578508809936 1104 9 -0.0634212995598844 1104 10-11 -0.14109557371529746 1104 12-13 -0.1395969460899451 1104 14-15 -0.17007947281700808 1104 16-17 -0.16263826674455117 1104 18-19 -0.1857379062520721 1104 20-21 -0.1075456135205286 1104 22-23 -0.20159400494715385 1104 24-25 -0.16453958408093428 1104 26-27 -0.23974100389820308 1104 28-29 -0.2348029672551064 1104 30-31 -0.22293239974906243 1104 32-33 -0.19223265520756883 1104 34-35 -0.18215978086004014 1104 36-37 -0.1862434993470714 1104 38-39 -0.23572485804887577 1104 40-41 -0.20088845030884528 1104 42-43 -0.24119054174634158 1104 44-45 -0.3098861398052719 1104 46-47 -0.2982448293558022 1104 48-49 -0.2734295214861291 1104 50-51 -0.3565682994112933 1104 52-53 -0.34663769627766783 1104 54-55 -0.33131172682801235 1104 56-57 -0.39432769304717397 1104 58-59 -0.3054790615823606 1104 60-61 -0.2775561514372882 1104 62-63 -0.16821162794893496 1104 64-65 -0.19847221371801282 1104 66-67 -0.10827982302159711 1104 68-69 -0.17441051416903264 1104 70-71 -0.17863693521908885 1104 72-73 -0.09570039627776339 1104 74-75 -0.04841578056365847 1104 76-77 -0.10810271688169593 1104 78-79 -0.1849892845245691 1104 80-81 -0.19591337626530247 1104 82-83 -0.20503281453374456 1104 84-85 -0.25707003996625133 1104 86-87 -0.2045915889457035 1104 88-89 -0.21623536111463437 1104 90-91 -0.2375087192147589 1104 92-93 -0.2204546372361733 1104 94-95 -0.17458243548277608 1104 96-97 -0.20551732808360867 1104 98-99 -0.2583919307635334 1104 100 -0.21190543519983507 1105 1 -0.34077671384163466 1105 2 -0.4531144976211827 1105 3 -0.3570716436219996 1105 4 -0.2838485637552637 1105 5 -0.20616308993317745 1105 6 -0.16544134982306247 1105 7 -0.14317688311682986 1105 8 -0.185758905727603 1105 9 -0.2855141856298786 1105 10-11 -0.21382392089811475 1105 12-13 -0.13545082862453484 1105 14-15 -0.24668643244793031 1105 16-17 -0.2687333433273267 1105 18-19 -0.30753270929413645 1105 20-21 -0.21994850888380313 1105 22-23 -0.29267736030969616 1105 24-25 -0.30443808359228797 1105 26-27 -0.26500771502286113 1105 28-29 -0.2951589970852808 1105 30-31 -0.30118571396167937 1105 32-33 -0.25719799417028355 1105 34-35 -0.2511638777666789 1105 36-37 -0.208401411007749 1105 38-39 -0.3035417962899274 1105 40-41 -0.26715934834968635 1105 42-43 -0.26470984784842244 1105 44-45 -0.25320630608739947 1105 46-47 -0.31242574981281024 1105 48-49 -0.3409095548233054 1105 50-51 -0.2805958229857026 1105 52-53 -0.3442896667822879 1105 54-55 -0.24765447478171865 1105 56-57 -0.31054899119938284 1105 58-59 -0.33113956448758586 1105 60-61 -0.2657580655702745 1105 62-63 -0.25862842894645155 1105 64-65 -0.34533751547817815 1105 66-67 -0.3683466445972243 1105 68-69 -0.2900006518983602 1105 70-71 -0.21297189818794493 1105 72-73 -0.2733869237198405 1105 74-75 -0.25454048879497293 1105 76-77 -0.23285858175044183 1105 78-79 -0.2757389946748958 1105 80-81 -0.194916856985067 1105 82-83 -0.23752100446061775 1105 84-85 -0.28889083431735685 1105 86-87 -0.1885779857267238 1105 88-89 -0.20252068171372883 1105 90-91 -0.17252024695652324 1105 92-93 -0.1637696511024771 1105 94-95 -0.11296721831686085 1105 96-97 -0.1915012849328619 1105 98-99 -0.15936922837821754 1105 100 -0.19263035276643947 1106 1 -0.10174558348695228 1106 2 -0.1373111878440909 1106 3 -0.10460831540841298 1106 4 -0.08024059697071806 1106 5 0.006029953569786528 1106 6 0.017204740469779267 1106 7 -0.0015989327871537284 1106 8 -0.00725399327390619 1106 9 -0.11367292345357782 1106 10-11 -0.04233454825973837 1106 12-13 -0.017370956530264436 1106 14-15 -0.03765701318403103 1106 16-17 0.0036367338205494093 1106 18-19 0.014210892207024983 1106 20-21 -0.021640764135256063 1106 22-23 0.031008430670759424 1106 24-25 0.009363452872534594 1106 26-27 0.022620760503471615 1106 28-29 0.020158376437713343 1106 30-31 0.041389065101334666 1106 32-33 0.0836812494870145 1106 34-35 -0.016623075691192923 1106 36-37 -0.01664433720292635 1106 38-39 0.0030937528721537433 1106 40-41 0.040793165837825995 1106 42-43 -0.08903154914607114 1106 44-45 -0.061895637605495324 1106 46-47 -0.06553022982719625 1106 48-49 -0.12196395871783494 1106 50-51 -0.09974160994881487 1106 52-53 -0.183957180878501 1106 54-55 -0.19358718495337968 1106 56-57 -0.1714984842633811 1106 58-59 -0.13533860154251443 1106 60-61 -0.16057778896873032 1106 62-63 -0.08994402780454891 1106 64-65 -0.14062193866813288 1106 66-67 -0.09459846305387742 1106 68-69 -0.08932622513068367 1106 70-71 -0.135738400576372 1106 72-73 -0.11610768655522463 1106 74-75 -0.04349002108555311 1106 76-77 -0.04027653224243011 1106 78-79 -0.0690629339169675 1106 80-81 -0.06930977482769407 1106 82-83 -0.0787517656795984 1106 84-85 -0.09838197756270262 1106 86-87 -0.03596275593670839 1106 88-89 -0.014244460262055725 1106 90-91 -0.07750763696144958 1106 92-93 -0.023100472581603526 1106 94-95 -0.07257502684241857 1106 96-97 -0.061356701143857606 1106 98-99 -0.042674359148161756 1106 100 0.006878029887563031 1107 1 -0.20548765803966162 1107 2 -0.1894053881828981 1107 3 -0.13445041239345557 1107 4 -0.10133601690661465 1107 5 0.06622312949860287 1107 6 0.008229227813558282 1107 7 0.014952134295839414 1107 8 0.04272099596114742 1107 9 -0.007535616932472067 1107 10-11 -0.03210456525158634 1107 12-13 -0.041947449505336465 1107 14-15 -0.1080915172102408 1107 16-17 -0.12784814498037633 1107 18-19 -0.10749735593070397 1107 20-21 -0.0700409131684836 1107 22-23 -0.05476205559524061 1107 24-25 -0.07262705592893326 1107 26-27 -0.11029334170031291 1107 28-29 -0.09268974375834205 1107 30-31 -0.16695247623217568 1107 32-33 -0.13046304715612678 1107 34-35 -0.12339428907426253 1107 36-37 -0.16492027040097668 1107 38-39 -0.164633787074159 1107 40-41 -0.14821995321084103 1107 42-43 -0.12956800426979953 1107 44-45 -0.17320050335955983 1107 46-47 -0.18885360612576108 1107 48-49 -0.05847490543974487 1107 50-51 -0.08558725743110784 1107 52-53 -0.1549149126857472 1107 54-55 -0.1680767848584921 1107 56-57 -0.025815548566839652 1107 58-59 -0.08067892843578761 1107 60-61 -0.08977424723814664 1107 62-63 -0.038149312037710104 1107 64-65 -0.09061336539045328 1107 66-67 -0.11604158419105914 1107 68-69 -0.051480038756643864 1107 70-71 -0.0623332880666112 1107 72-73 -0.10641653082582536 1107 74-75 -0.02490934331960659 1107 76-77 -0.015009922644999563 1107 78-79 -0.05619309045341225 1107 80-81 -0.08614853016826629 1107 82-83 -0.06122946871157353 1107 84-85 -0.03948994155751251 1107 86-87 -0.11384846963306927 1107 88-89 -0.04290060865582035 1107 90-91 -0.013803864977120384 1107 92-93 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0.27101960971258166 2214 74-75 0.27027914184391477 2214 76-77 0.2833974649679547 2214 78-79 0.2818246122898529 2214 80-81 0.24540218442482598 2214 82-83 0.24638609899723463 2214 84-85 0.2767248219544314 2214 86-87 0.2514671650243514 2214 88-89 0.19633677096072688 2214 90-91 0.1877855952120413 2214 92-93 0.22134934050620814 2214 94-95 0.23460602883093173 2214 96-97 0.23380834033542897 2214 98-99 0.29312646522527075 2214 100 0.34738824907415733 2215 1 0.16572524902244012 2215 2 0.09743273147566356 2215 3 0.04024040913182958 2215 4 -0.08745831498534784 2215 5 -0.09638254395839141 2215 6 -0.10134380609933658 2215 7 -0.009666957307622681 2215 8 -0.06760598061818257 2215 9 -0.08891516193488513 2215 10-11 -0.11004267560796421 2215 12-13 -0.11086512303214135 2215 14-15 -0.1015881976807691 2215 16-17 -0.05394681373729071 2215 18-19 -0.08716344040124113 2215 20-21 -0.07106730160806762 2215 22-23 -0.07790001307058958 2215 24-25 -0.13093712058999074 2215 26-27 -0.07969286417519328 2215 28-29 -0.139600373809472 2215 30-31 -0.13814425504894956 2215 32-33 -0.10208106018275487 2215 34-35 -0.14176644132298577 2215 36-37 -0.1459107006595204 2215 38-39 -0.10959163372069014 2215 40-41 -0.08173006928181081 2215 42-43 -0.03821090449509512 2215 44-45 0.008393071115101236 2215 46-47 0.023059843338707253 2215 48-49 0.03175366042395211 2215 50-51 0.06465427694065085 2215 52-53 0.07013615509143989 2215 54-55 0.030873958016762515 2215 56-57 0.083860424180358 2215 58-59 0.10038415898745257 2215 60-61 0.08508169293754264 2215 62-63 0.044285752697724945 2215 64-65 0.08419514656026905 2215 66-67 0.009979349878697974 2215 68-69 -0.0599609888245638 2215 70-71 -0.06579574682908884 2215 72-73 -0.10099002786429878 2215 74-75 -0.08475954847333611 2215 76-77 -0.10110310926437904 2215 78-79 -0.16078727641251334 2215 80-81 -0.15251127769500528 2215 82-83 -0.14279171031080296 2215 84-85 -0.15837802264871925 2215 86-87 -0.21504126097921983 2215 88-89 -0.19873131017306633 2215 90-91 -0.16892547362350285 2215 92-93 -0.23700199522348697 2215 94-95 -0.19969584946452557 2215 96-97 -0.22747297561488722 2215 98-99 -0.15613095396722443 2215 100 -0.16609013305489384 2216 1 0.5156461165044419 2216 2 0.37940406055577824 2216 3 0.20687049144927983 2216 4 0.10095129906206779 2216 5 0.06002620089053323 2216 6 0.0737001693028958 2216 7 0.10622392967428596 2216 8 0.08740709349962827 2216 9 0.08278893502274087 2216 10-11 0.06579711400704724 2216 12-13 0.009239634254605278 2216 14-15 0.0141038974292087 2216 16-17 0.003271406440333635 2216 18-19 0.03713725008047675 2216 20-21 0.05201807835013028 2216 22-23 0.0612301592177289 2216 24-25 0.0549786835673558 2216 26-27 0.055642953140349505 2216 28-29 0.021601041255408404 2216 30-31 0.028380106741828115 2216 32-33 -0.012836938742395887 2216 34-35 -0.03831922934332255 2216 36-37 0.06119497413988029 2216 38-39 0.03389084607885451 2216 40-41 0.0572125717592229 2216 42-43 0.07353789866408533 2216 44-45 0.05069472985837109 2216 46-47 0.14321131700068435 2216 48-49 0.1706941330338978 2216 50-51 0.14772681522163822 2216 52-53 0.1250398033283986 2216 54-55 0.12653005412710883 2216 56-57 0.09266214306906306 2216 58-59 0.05749748035966462 2216 60-61 0.09820070107736711 2216 62-63 0.10404931153597374 2216 64-65 0.035104833839788796 2216 66-67 0.10391030688037972 2216 68-69 -0.03344874209636828 2216 70-71 -0.11699221482764699 2216 72-73 -0.10258597413072579 2216 74-75 -0.03678519789372103 2216 76-77 -0.1027449920316954 2216 78-79 -0.059850175598811006 2216 80-81 -0.08455713613236782 2216 82-83 -0.08845991920117058 2216 84-85 -0.07065003399334557 2216 86-87 -0.0575184561219686 2216 88-89 -0.07978415270852679 2216 90-91 -0.002782584513951747 2216 92-93 -0.0554906740729848 2216 94-95 -0.011227573649527756 2216 96-97 -0.05021181745811987 2216 98-99 0.005101827263263914 2216 100 0.042813247209629424 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33490.0 3 5225.0 4 3345.0 5 3014.0 6 2688.0 7 2737.0 8 2868.0 9 3089.0 10 3524.0 11 3785.0 12 4122.0 13 4345.0 14 4537.0 15 4841.0 16 4982.0 17 5168.0 18 5027.0 19 5146.0 20 5501.0 21 5715.0 22 6827.0 23 8087.0 24 10018.0 25 13164.0 26 16190.0 27 17251.0 28 19756.0 29 24465.0 30 28665.0 31 32197.0 32 40636.0 33 55189.0 34 82242.0 35 137956.0 36 293035.0 37 635484.0 38 858313.0 39 311628.0 40 654.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.84734129062491 18.296269917131617 13.92182076499964 23.934568027243827 2 16.05168199709903 18.933219924717246 39.104838207333096 25.91025987085062 3 19.449426175077686 23.40218688602513 28.353955040957302 28.79443189793988 4 13.741098590755438 15.686578432521348 35.56002675799588 35.012296218727336 5 15.287713570363703 36.463801623844766 33.4428675293705 14.805617276421032 6 34.09206041673147 34.68696258837436 16.83086188789695 14.39011510699722 7 30.259132542235704 30.510042121857566 20.29128021642571 18.939545119481018 8 27.05071812986382 34.72695961458871 19.018021546487237 19.204300709060227 9 26.31151234180531 16.43121883819575 19.51523552372564 37.742033296273306 10-11 25.964261850247013 25.690228647899264 26.84035367959679 21.50515582225694 12-13 26.12752430884477 22.74381307648712 27.135211852595504 23.993450762072612 14-15 23.415312728636966 24.653693366744754 24.14985684653716 27.781137058081125 16-17 23.904297747251455 27.863704261953202 24.626138205370847 23.605859785424492 18-19 24.069616319568475 27.153947835511698 25.944246385239566 22.832189459680258 20-21 24.744714984254486 26.175814082799953 25.789011032133143 23.290459900812422 22-23 24.6528799717158 25.9719453344958 25.689658683044847 23.68551601074355 24-25 24.395694945953615 26.041767886224683 25.62062049000353 23.94191667781817 26-27 24.11050608519026 26.26381552803067 25.706335731328757 23.919342655450315 28-29 24.117712279644408 26.260957737594033 25.75402210214937 23.867307880612188 30-31 24.18338770721502 26.393816942848474 25.531466366143547 23.891328983792963 32-33 24.1084766892157 26.26114681744759 25.727624563787998 23.902751929548707 34-35 24.207137983482745 26.100106168330008 25.776160444644887 23.916595403542363 36-37 24.37796286377499 26.167479836702178 25.590240775076094 23.86431652444674 38-39 24.210766509473245 26.283132483297045 25.56919231006967 23.936908697160046 40-41 24.261286352743053 26.14177454128228 25.73590073376329 23.861038372211375 42-43 24.2813962544066 26.18476711079394 25.662452417579328 23.871384217220136 44-45 24.115411506358924 26.28552594517789 25.663938725135495 23.935123823327693 46-47 24.300963548110502 26.139650162984967 25.685701906941716 23.87368438196281 48-49 24.331514582209106 26.149506968178148 25.53809347211211 23.980884977500637 50-51 24.1332650348056 26.309595893797766 25.60352778674278 23.953611284653853 52-53 24.249970435757504 26.211574118712598 25.5917706473616 23.946684798168295 54-55 24.250563656077908 26.248246295359674 25.609593377404273 23.89159667115815 56-57 24.183127492812115 26.19331119474359 25.66260315467501 23.960958157769284 58-59 24.156144829826157 26.035482918767567 25.702954318957627 24.10541793244865 60-61 24.298249055609304 26.127180167462328 25.553605784712236 24.020964992216136 62-63 24.10612248977068 26.31988245790891 25.62605247383054 23.947942578489865 64-65 24.269616053743924 26.0809123528161 25.60886533465317 24.040606258786806 66-67 24.132763010127974 26.056178760494607 25.665062447577867 24.145995781799552 68-69 24.14287282022754 26.138504253550828 25.68425178533656 24.03437114088507 70-71 24.198695881989757 25.97816253799606 25.703676641511166 24.119464938503018 72-73 24.1557944450933 26.133830172897426 25.737101362415675 23.9732740195936 74-75 24.103999495025874 26.181149841631008 25.674842940644993 24.040007722698125 76-77 24.204976230074664 26.103238802704993 25.56287694015561 24.128908027064732 78-79 24.23414821215957 26.1465589950418 25.666119621963546 23.953173170835083 80-81 24.098207881987662 26.145562738012057 25.765779939198474 23.990449440801804 82-83 24.149877103040787 26.117024259402623 25.718916211868027 24.014182425688567 84-85 24.251885694486397 26.015513483418918 25.606280021559257 24.126320800535428 86-87 24.204828695605183 26.25510700371102 25.610212452834936 23.92985184784886 88-89 24.07801660182916 26.209645124074143 25.664011700180634 24.04832657391606 90-91 24.121246166628957 26.139611308197626 25.62561249133799 24.113530033835428 92-93 24.19099698969988 26.25547710749439 25.581715749889494 23.971810152916238 94-95 24.22158910481139 26.05157563630444 25.71069542658135 24.016139832302823 96-97 24.225476403250568 26.149421214621288 25.607970812328524 24.01713156979962 98-99 24.122410797608985 26.27230572636419 25.632329717072267 23.972953758954553 100 24.393962960600646 25.93477840031063 25.52716671459685 24.144091924491875 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2824.0 1 1722.5 2 565.0 3 451.0 4 336.0 5 263.5 6 242.0 7 230.0 8 229.0 9 238.5 10 223.0 11 197.0 12 192.5 13 187.5 14 192.5 15 213.5 16 238.5 17 283.0 18 384.0 19 511.0 20 741.0 21 1114.5 22 1650.5 23 2470.0 24 3664.0 25 5292.0 26 7562.0 27 10576.0 28 14301.0 29 18887.0 30 23300.5 31 27716.5 32 33058.0 33 37857.5 34 42228.5 35 46966.0 36 52427.5 37 58383.5 38 64687.0 39 69997.0 40 74622.0 41 79657.0 42 82951.0 43 85216.0 44 87818.0 45 90342.0 46 92491.5 47 92136.0 48 91669.5 49 92983.0 50 93809.0 51 94720.0 52 94247.5 53 94548.5 54 95421.0 55 92691.0 56 87676.0 57 81681.5 58 75624.5 59 71310.5 60 66749.0 61 59926.5 62 53224.5 63 46963.0 64 39926.5 65 33569.5 66 27666.5 67 22160.0 68 17403.5 69 13347.5 70 10110.0 71 7556.5 72 5847.5 73 4532.5 74 3583.5 75 2769.5 76 1996.5 77 1407.5 78 1016.0 79 736.0 80 488.5 81 271.5 82 175.0 83 121.0 84 97.0 85 81.5 86 63.0 87 47.0 88 40.5 89 35.5 90 31.0 91 27.5 92 22.5 93 18.0 94 15.5 95 15.5 96 12.0 97 7.5 98 7.0 99 5.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7544809320545705 2 0.29110068889639784 3 0.2894370451320674 4 0.30100861175952137 5 0.2856661192662518 6 0.16495952169872077 7 0.14540246500248066 8 0.13249998336356236 9 0.14355397193100242 10-11 0.1327587723935693 12-13 0.13024482181635885 14-15 0.12697298907984234 16-17 0.12346085224403361 18-19 0.1261781370591067 20-21 0.1353281777629241 22-23 0.13867395022229978 24-25 0.12852572325988407 26-27 0.13133543272853104 28-29 0.13588272568436757 30-31 0.1254941946226597 32-33 0.11970841130893274 34-35 0.11329414035090314 36-37 0.12344236731331884 38-39 0.11604839502740574 40-41 0.11390414306449097 42-43 0.12118720576611536 44-45 0.11508717863023706 46-47 0.121131750973971 48-49 0.11283201708303357 50-51 0.1124623184687379 52-53 0.11691718677100055 54-55 0.11554930189810664 56-57 0.11601142516597619 58-59 0.11769355386102143 60-61 0.11697264156314491 62-63 0.11961598665535883 64-65 0.11299838145946663 66-67 0.12126114548897447 68-69 0.11477293480808574 70-71 0.12237024133186145 72-73 0.13177907106568584 74-75 0.13965365155018325 76-77 0.12506904121621973 78-79 0.1264554110198284 80-81 0.1291542109041867 82-83 0.12024447429966142 84-85 0.12991209306349277 86-87 0.11919083324891881 88-89 0.7579930688903792 90-91 0.8212854716577952 92-93 0.8483658951549518 94-95 0.8675162833754666 96-97 0.8789214856264875 98-99 0.8823966526008667 100 0.8844299949794928 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2704906.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.432656738639146 #Duplication Level Percentage of deduplicated Percentage of total 1 79.77525784188681 49.80581289078951 2 11.49045569279721 14.347593520779006 3 3.5345496381160335 6.620139728465387 4 1.6408738042457955 4.0977644388761085 5 0.9298765583345701 2.9027331987904685 6 0.5884327795048037 2.204245304195205 7 0.40362716299507184 1.7639661282363452 8 0.28432387567891876 1.4200875946289158 9 0.19528045076188377 1.0972689615165072 >10 1.091057507475123 12.02092814147202 >50 0.05133165085085803 2.150205366846972 >100 0.014695341790024633 1.4168111799640588 >500 1.7827167215059525E-4 0.06696534425006141 >1k 5.942389071476985E-5 0.08547820118944431 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.6969861429565386E-5 0.0 12-13 0.0 1.8484930714782693E-5 0.0 3.6969861429565386E-5 0.0 14-15 0.0 7.393972285913077E-5 0.0 3.6969861429565386E-5 0.0 16-17 0.0 1.1090958428869617E-4 0.0 3.6969861429565386E-5 0.0 18-19 0.0 1.1090958428869617E-4 0.0 3.6969861429565386E-5 0.0 20-21 0.0 1.1090958428869617E-4 0.0 3.6969861429565386E-5 0.0 22-23 0.0 1.1090958428869617E-4 0.0 7.393972285913077E-5 0.0 24-25 0.0 1.1090958428869617E-4 0.0 1.1090958428869617E-4 0.0 26-27 0.0 1.1090958428869617E-4 0.0 3.696986142956539E-4 0.0 28-29 0.0 1.2939451500347884E-4 0.0 0.0010166711893130481 0.0 30-31 0.0 1.6636437643304425E-4 0.0 0.002661830022928708 0.0 32-33 0.0 1.8484930714782695E-4 0.0 0.007172153117335685 0.0 34-35 0.0 1.8484930714782695E-4 0.0 0.013420059698932237 0.0 36-37 0.0 1.8484930714782695E-4 0.0 0.022681009987038366 0.0 38-39 0.0 2.0333423786260966E-4 0.0 0.03397530265377059 0.0 40-41 0.0 2.4030409929217504E-4 0.0 0.04480747205263325 0.0 42-43 0.0 2.5878903000695774E-4 0.0 0.05619418937293939 0.0 44-45 0.0 2.5878903000695774E-4 0.0 0.07005788740902641 0.0 46-47 0.0 2.5878903000695774E-4 0.0 0.08684220449804911 0.0 48-49 0.0 2.772739607217404E-4 0.0 0.10273924491276222 0.0 50-51 0.0 2.957588914365231E-4 0.0 0.11769355386102143 0.0 52-53 0.0 2.957588914365231E-4 0.0 0.1316127066892528 0.0 54-55 0.0 2.957588914365231E-4 0.0 0.14823065940184244 0.0 56-57 0.0 2.957588914365231E-4 0.0 0.16649377094804774 0.0 58-59 0.0 2.957588914365231E-4 0.0 0.18592143312928433 0.0 60-61 0.0 3.327287528660885E-4 0.0 0.2069203144212775 0.0 62-63 0.0 3.327287528660885E-4 0.0 0.22673616014752454 0.0 64-65 0.0 3.327287528660885E-4 0.0 0.2467553401116342 0.0 66-67 0.0 3.327287528660885E-4 0.0 0.2680869501564934 0.0 68-69 0.0 3.327287528660885E-4 0.0 0.2922467546007144 0.0 70-71 0.0 3.327287528660885E-4 0.0 0.31710898641209717 0.0 72-73 0.0 3.327287528660885E-4 0.0 0.34259970586778243 0.0 74-75 0.0 3.327287528660885E-4 0.0 0.3669073897577217 0.0 76-77 0.0 3.327287528660885E-4 0.0 0.3933223557491462 0.0 78-79 0.0 3.327287528660885E-4 0.0 0.42389643115139675 0.0 80-81 0.0 3.327287528660885E-4 0.0 0.4561711201794073 0.0 82-83 0.0 3.327287528660885E-4 0.0 0.4863570120366475 0.0 84-85 0.0 3.696986142956539E-4 0.0 0.5180216983510701 0.0 86-87 0.0 4.4363833715478466E-4 0.0 0.5510357846076721 0.0 88 0.0 4.4363833715478466E-4 0.0 0.5802419751370288 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2465 0.0 37.94449 1 GTATCAA 3875 0.0 37.547363 1 TCAACGC 4310 0.0 33.287582 4 ATCAACG 4435 0.0 32.34998 3 CAACGCA 4805 0.0 29.853392 5 TATCAAC 4900 0.0 29.184574 2 AACGCAG 4945 0.0 28.966295 6 GTACATG 10375 0.0 26.453823 1 TACATGG 10665 0.0 25.229637 2 ACGCAGA 5765 0.0 24.678701 7 ACATGGG 11090 0.0 23.668484 3 CGCAGAG 6325 0.0 22.270174 8 GTATAGA 795 0.0 20.79703 1 CATGGGG 9630 0.0 20.466684 4 GCAGAGT 7385 0.0 19.073643 9 GAGTACA 7120 0.0 18.975204 1 AGAGTAC 6210 0.0 17.426495 10-11 AGTACAT 7465 0.0 17.203178 2 GTCGTAC 340 1.8757419E-8 16.672577 1 CAGAGTA 6770 0.0 16.331758 10-11 >>END_MODULE