FastQCFastQC Report
Wed 25 May 2016
SRR2049392_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049392_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2704906
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48640.17982140599340607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37820.1398200159266163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31000.050.3100471
GTATCAA59350.043.505911
ATCAACG74500.034.3963243
TCAACGC74750.034.2183844
TATCAAC77100.033.171122
CAACGCA78600.032.4824755
AACGCAG82100.031.2024426
ACGCAGA97000.026.407547
CGCAGAG99950.025.58118
GTACATG97000.024.9677721
TACATGG99250.023.6366312
GCAGAGT114100.022.3263029
ACATGGG102000.022.3108943
AGAGTAC101950.020.37426810-11
TACCGTC3800.019.7897157
CGTATAC1456.5852836E-419.4560663
TATAACG1708.614961E-519.3581892
CATGGGG88700.019.0831764
CAGAGTA106600.018.8462910-11
ATACCGT4750.018.801626
Produced by FastQC (version 0.11.5)