##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049392_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2704906
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.000897628235506	34.0	33.0	34.0	31.0	34.0
2	33.14006549580651	34.0	33.0	34.0	31.0	34.0
3	33.25676382099785	34.0	34.0	34.0	31.0	34.0
4	36.58722077587909	37.0	37.0	37.0	35.0	37.0
5	36.54264954124099	37.0	37.0	37.0	35.0	37.0
6	36.56574128638851	37.0	37.0	37.0	35.0	37.0
7	36.54895068442305	37.0	37.0	37.0	35.0	37.0
8	36.54894661773829	37.0	37.0	37.0	35.0	37.0
9	38.370389211307156	39.0	39.0	39.0	37.0	39.0
10-11	38.38435605525663	39.0	39.0	39.0	37.0	39.0
12-13	38.37775915318314	39.0	39.0	39.0	37.0	39.0
14-15	40.03186598720991	41.0	40.0	41.0	38.0	41.0
16-17	40.03469621495165	41.0	40.0	41.0	38.0	41.0
18-19	40.03354441891881	41.0	40.0	41.0	38.0	41.0
20-21	40.02494356550653	41.0	40.0	41.0	38.0	41.0
22-23	39.99848867206476	41.0	40.0	41.0	38.0	41.0
24-25	39.97120435978182	41.0	40.0	41.0	38.0	41.0
26-27	39.90819200371473	41.0	40.0	41.0	38.0	41.0
28-29	39.84350472807558	41.0	40.0	41.0	38.0	41.0
30-31	39.76782390959242	41.0	40.0	41.0	37.0	41.0
32-33	39.67774887556166	41.0	40.0	41.0	37.0	41.0
34-35	39.60527371376307	41.0	40.0	41.0	36.5	41.0
36-37	39.52175454525961	41.0	40.0	41.0	36.0	41.0
38-39	39.436804827968146	41.0	40.0	41.0	36.0	41.0
40-41	39.33335871930485	41.0	40.0	41.0	35.0	41.0
42-43	39.21895696190552	41.0	39.0	41.0	35.0	41.0
44-45	39.077914722360035	41.0	39.0	41.0	35.0	41.0
46-47	38.88663321387139	41.0	39.0	41.0	35.0	41.0
48-49	38.68289951665603	41.0	39.0	41.0	35.0	41.0
50-51	38.506922236854074	41.0	38.0	41.0	35.0	41.0
52-53	38.35784884946094	40.0	38.0	41.0	35.0	41.0
54-55	38.242286977809954	40.0	37.0	41.0	35.0	41.0
56-57	38.08661983078155	40.0	37.0	41.0	35.0	41.0
58-59	37.862819447330146	40.0	36.0	41.0	35.0	41.0
60-61	37.61197043446242	39.5	36.0	41.0	34.0	41.0
62-63	37.33833929903664	39.0	35.0	41.0	34.0	41.0
64-65	37.04824511461766	39.0	35.0	41.0	34.0	41.0
66-67	36.733640281769496	37.5	35.0	41.0	34.0	41.0
68-69	36.406220585854	37.0	35.0	40.0	34.0	41.0
70-71	36.072794581401354	36.5	35.0	39.0	33.0	41.0
72-73	35.73541224722781	36.0	35.0	39.0	33.0	41.0
74-75	35.3957263209886	35.5	35.0	39.0	33.0	41.0
76-77	35.084502197118866	35.0	35.0	37.0	33.0	39.5
78-79	34.78185009016949	35.0	35.0	37.0	33.0	39.0
80-81	34.50827311559071	35.0	35.0	36.5	33.0	39.0
82-83	34.26894964187295	35.0	35.0	36.0	33.0	37.5
84-85	34.048362863626316	35.0	35.0	36.0	32.0	37.0
86-87	33.848825245683216	35.0	35.0	35.5	32.0	37.0
88-89	33.70743105305692	35.0	35.0	35.0	32.0	36.0
90-91	33.588271089642305	35.0	35.0	35.0	32.0	36.0
92-93	33.47848483459315	35.0	35.0	35.0	32.0	36.0
94-95	33.389227019349285	35.0	35.0	35.0	32.0	36.0
96-97	33.372505181326076	35.0	35.0	35.0	32.0	36.0
98-99	33.31941923305283	35.0	35.0	35.0	32.0	35.0
100	33.268599722134525	35.0	35.0	35.0	32.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.048310428211536305
1101	2	0.002762409303478819
1101	3	0.006948602140639082
1101	4	-0.002580496362675433
1101	5	0.01890555656648729
1101	6	-0.027405942531643745
1101	7	-0.019550930879105977
1101	8	0.017142371620622043
1101	9	0.028594634103107808
1101	10-11	-0.02008776398060519
1101	12-13	0.04652628366552847
1101	14-15	-0.004177211950946003
1101	16-17	-0.037363462395767044
1101	18-19	-0.02006932743743306
1101	20-21	-0.055761520033016154
1101	22-23	-0.0688820498698135
1101	24-25	-0.011838170650882773
1101	26-27	-5.913917434909877E-4
1101	28-29	-0.10459384123485904
1101	30-31	-0.08261326463127716
1101	32-33	-0.02168844375794521
1101	34-35	-0.0657168905431007
1101	36-37	-0.06843084221431184
1101	38-39	-0.017257973554031025
1101	40-41	0.025393687999368808
1101	42-43	-0.011974707561186904
1101	44-45	0.006174402641647703
1101	46-47	0.014322344748869398
1101	48-49	-0.011035681673014608
1101	50-51	-0.13478859880932959
1101	52-53	-0.027239125049256074
1101	54-55	-0.03337044137070677
1101	56-57	-0.0403841073880713
1101	58-59	-0.02438132894241818
1101	60-61	-0.10062134883138896
1101	62-63	0.015643421696601933
1101	64-65	-0.0771152333282501
1101	66-67	-0.016010606020479656
1101	68-69	0.02690427068255019
1101	70-71	-0.02862115609255511
1101	72-73	-0.0715910650949283
1101	74-75	-0.03432497409561108
1101	76-77	0.020356735180250496
1101	78-79	-0.0812747579873232
1101	80-81	-0.06683296060974442
1101	82-83	-0.10888310292282455
1101	84-85	-0.0813084281901908
1101	86-87	-0.06739669241122215
1101	88-89	-0.04789325844873815
1101	90-91	-0.06891719764021076
1101	92-93	-0.00282285044212216
1101	94-95	-0.05297500761702878
1101	96-97	-0.12259726541751093
1101	98-99	-0.14569961873605308
1101	100	-0.10104155044083285
1102	1	-0.16328678573378852
1102	2	-0.1261324516789415
1102	3	-0.140733431887611
1102	4	-0.0917175824817349
1102	5	-0.0894972440211248
1102	6	-0.17242663530112878
1102	7	-0.03517557955957784
1102	8	-0.06018165178773671
1102	9	0.0334585866712942
1102	10-11	-0.02605101747108307
1102	12-13	-0.008169263517146419
1102	14-15	-0.008883380284629538
1102	16-17	-0.06831566627280239
1102	18-19	-9.822231413636473E-5
1102	20-21	0.03656757181061465
1102	22-23	0.0019983704353094822
1102	24-25	0.015701349700762535
1102	26-27	0.025675673836104806
1102	28-29	0.015071035114033293
1102	30-31	-0.016579697705999763
1102	32-33	0.022153914461483737
1102	34-35	-0.03993441989199198
1102	36-37	-0.10531143296100254
1102	38-39	-0.1076563901456069
1102	40-41	-0.08304948239613452
1102	42-43	-0.08922467379519361
1102	44-45	-0.1479096068106216
1102	46-47	-0.038071730299826356
1102	48-49	-0.057410488648727664
1102	50-51	-0.14708126427168366
1102	52-53	-0.045157855869661034
1102	54-55	-0.0018468101351913901
1102	56-57	-0.047235811119755056
1102	58-59	0.017173826120881586
1102	60-61	-0.03764633534465389
1102	62-63	-0.04854417974875247
1102	64-65	-0.05297927693487736
1102	66-67	-0.08434336518796215
1102	68-69	-0.08062360178061567
1102	70-71	-0.02805586085679579
1102	72-73	-0.06655211117845994
1102	74-75	-0.07613960442846235
1102	76-77	-0.08072635492532498
1102	78-79	-0.12224197613098653
1102	80-81	-0.118332885687785
1102	82-83	-0.14148390440698222
1102	84-85	-0.030531425348499397
1102	86-87	-0.05325644982130484
1102	88-89	-0.09211474694762956
1102	90-91	-0.013927740960433255
1102	92-93	-0.03257956554888608
1102	94-95	-0.06809800148915457
1102	96-97	-0.02730910989556179
1102	98-99	-0.01843307218561563
1102	100	0.03295287813259762
1103	1	-0.15205033173516824
1103	2	-0.07922309690479068
1103	3	-0.0681899777019197
1103	4	-0.03466732355629176
1103	5	-0.06095517425396224
1103	6	-0.02737906223982378
1103	7	-0.037171308759013755
1103	8	-0.023805632344412686
1103	9	-0.05686058633454394
1103	10-11	-0.05777917052846249
1103	12-13	-0.10126756837271245
1103	14-15	-0.13554058189230744
1103	16-17	-0.09299573076119572
1103	18-19	-0.08541620258425553
1103	20-21	-0.09405761761540532
1103	22-23	-0.13454397898132697
1103	24-25	-0.10122938980690321
1103	26-27	-0.12449401730872012
1103	28-29	-0.09995029007873768
1103	30-31	-0.1303838178492498
1103	32-33	-0.11751017581919854
1103	34-35	-0.1429385389596689
1103	36-37	-0.1177418903223284
1103	38-39	-0.1563970648153159
1103	40-41	-0.10409256723709603
1103	42-43	-0.16084985592267742
1103	44-45	-0.16495566331134626
1103	46-47	-0.1274315673348596
1103	48-49	-0.13745446429617658
1103	50-51	-0.1304892540260667
1103	52-53	-0.13781636583735946
1103	54-55	-0.15745568347433903
1103	56-57	-0.15885071382439975
1103	58-59	-0.19328371959392854
1103	60-61	-0.260723370706863
1103	62-63	-0.2775361471250335
1103	64-65	-0.2294478495970722
1103	66-67	-0.24374575273373011
1103	68-69	-0.23996080180566537
1103	70-71	-0.17949603642134093
1103	72-73	-0.15921457772520142
1103	74-75	-0.13833545201796227
1103	76-77	-0.1118584858427667
1103	78-79	-0.21942128099462366
1103	80-81	-0.22582449524335146
1103	82-83	-0.18791524297145656
1103	84-85	-0.08556240766719725
1103	86-87	-0.10185580163378205
1103	88-89	-0.15545124170285618
1103	90-91	-0.17954088202706942
1103	92-93	-0.24501709097375368
1103	94-95	-0.2686692469933476
1103	96-97	-0.2422210337571542
1103	98-99	-0.2279000902579682
1103	100	-0.32871993275560385
1104	1	-0.1234766474726996
1104	2	-0.0721749082951817
1104	3	-0.03444512173484071
1104	4	-0.05711257722989416
1104	5	-0.021693743892079453
1104	6	-0.04614528383045524
1104	7	-0.013895114899284522
1104	8	0.019608525218423267
1104	9	-0.01591753830383169
1104	10-11	0.006311778934197321
1104	12-13	-0.04434027070468005
1104	14-15	-0.090505005415217
1104	16-17	-0.07097482896411833
1104	18-19	-0.07959172636276435
1104	20-21	0.01881800781018228
1104	22-23	-0.05088843428128342
1104	24-25	-0.07037999524848004
1104	26-27	-0.08653031419714097
1104	28-29	-0.01138706658345967
1104	30-31	-0.019451329272214934
1104	32-33	-0.0013147371084443193
1104	34-35	-0.009000257486079022
1104	36-37	0.016928644854466768
1104	38-39	-0.016435947289579644
1104	40-41	-0.021497902228361454
1104	42-43	-0.08417440615902905
1104	44-45	-0.031149621724850363
1104	46-47	0.014752956650887938
1104	48-49	0.033448813081925266
1104	50-51	-9.208100158488719E-4
1104	52-53	-0.005232062909335866
1104	54-55	-0.004044585192474415
1104	56-57	-0.028105926937861625
1104	58-59	-0.11895306066428901
1104	60-61	-0.05278404315655649
1104	62-63	0.026779412772725664
1104	64-65	0.029605154912907494
1104	66-67	-0.01861918731938772
1104	68-69	-0.04464051482536746
1104	70-71	-0.053192141265867576
1104	72-73	-0.09249659408741451
1104	74-75	-0.08467369006949355
1104	76-77	-0.03787322960331352
1104	78-79	-0.0983467825686688
1104	80-81	-0.09265202087716062
1104	82-83	-0.014447103902462288
1104	84-85	-0.04643223032759636
1104	86-87	-0.004291384032065082
1104	88-89	-0.048566872654888016
1104	90-91	-0.005318778228627252
1104	92-93	0.016512944257328854
1104	94-95	0.018366309746447484
1104	96-97	-0.004622470632341447
1104	98-99	-0.06562891954021666
1104	100	-0.017097625663005545
1105	1	-0.19165740936147557
1105	2	-0.14616822613761826
1105	3	-0.11854579699573264
1105	4	-0.008623928001945558
1105	5	-0.06412609635265198
1105	6	-0.06379009983768213
1105	7	-0.04568888535516891
1105	8	-0.05312150438588503
1105	9	-0.047021936553186094
1105	10-11	-0.06533761031526097
1105	12-13	-0.09459020493250847
1105	14-15	-0.07142760750718224
1105	16-17	-0.04494177484966144
1105	18-19	-0.0630486861303865
1105	20-21	-0.08680546499800101
1105	22-23	-0.09182216979747437
1105	24-25	-0.07257870983639947
1105	26-27	-0.03025825738849619
1105	28-29	-0.08836237136988245
1105	30-31	-0.05248662163815965
1105	32-33	-0.022664981301403486
1105	34-35	-0.06622729879437372
1105	36-37	-0.145660252719928
1105	38-39	-0.15807606849963918
1105	40-41	-0.025861367520320755
1105	42-43	-0.06890275081821784
1105	44-45	-0.05275527853063977
1105	46-47	-0.07067630583231477
1105	48-49	-0.15313162527720436
1105	50-51	-0.13221351329074338
1105	52-53	-0.17176487191600387
1105	54-55	-0.18010252862988096
1105	56-57	-0.14033490197935095
1105	58-59	-0.11948680888275476
1105	60-61	-0.12261200083776913
1105	62-63	-0.12059639875904793
1105	64-65	-0.16524144084390713
1105	66-67	-0.1520388919660931
1105	68-69	-0.19862719130895812
1105	70-71	-0.14945304362001366
1105	72-73	-0.10355367843835239
1105	74-75	-0.12302943010256229
1105	76-77	-0.14465200022006997
1105	78-79	-0.16089563851154054
1105	80-81	-0.12043258516553834
1105	82-83	-0.1470681840969803
1105	84-85	-0.17125265812111223
1105	86-87	-0.10318380390305748
1105	88-89	-0.09737349954490071
1105	90-91	-0.16915992315010442
1105	92-93	-0.12927886743933215
1105	94-95	-0.1927483554800702
1105	96-97	-0.18445271850981015
1105	98-99	-0.13232441870520972
1105	100	-0.1020912461310104
1106	1	-0.05594904145126378
1106	2	-0.052450499304804055
1106	3	-0.07877531310873565
1106	4	-0.006299414563798678
1106	5	-0.046158696351703554
1106	6	-0.06042499973211335
1106	7	-0.08350304466515723
1106	8	-0.07482280204195746
1106	9	-0.041154315463082014
1106	10-11	-0.011109964904669312
1106	12-13	-0.048239156737238886
1106	14-15	-0.1011288970474098
1106	16-17	-0.12093801180260044
1106	18-19	-0.046464192400215154
1106	20-21	-0.04752223618999807
1106	22-23	-0.03408097193924675
1106	24-25	-0.06658796460564531
1106	26-27	-0.08281650717934497
1106	28-29	-0.14131095951709938
1106	30-31	-0.178562292429703
1106	32-33	-0.10406410573693137
1106	34-35	-0.16538274361371919
1106	36-37	-0.1786077452279855
1106	38-39	-0.146680440640921
1106	40-41	-0.15300346042104707
1106	42-43	-0.16307320657028157
1106	44-45	-0.1679258905127412
1106	46-47	-0.24279137634886894
1106	48-49	-0.23789243439996
1106	50-51	-0.08795202480408193
1106	52-53	-0.08620783070983151
1106	54-55	-0.12033842244485982
1106	56-57	-0.09347803370831542
1106	58-59	-0.0601695476289521
1106	60-61	-0.0830727171017216
1106	62-63	-0.10714056349989676
1106	64-65	-0.11454293706766805
1106	66-67	-0.07474641920997271
1106	68-69	-0.08495253256336355
1106	70-71	-0.042128053375940056
1106	72-73	-0.06256318006456496
1106	74-75	-0.06774327553223003
1106	76-77	-0.13485350313940359
1106	78-79	-0.045511004918296294
1106	80-81	-0.02373796617404622
1106	82-83	-0.047484290263057005
1106	84-85	-0.04227689135969115
1106	86-87	-0.009761832009580473
1106	88-89	-0.06899005424671856
1106	90-91	-0.10087975875012489
1106	92-93	-0.021537027076661275
1106	94-95	-0.013254391978378521
1106	96-97	-0.0881821524498676
1106	98-99	-0.09089302702030011
1106	100	-0.04831186592461023
1107	1	-0.1328508264979149
1107	2	-0.09095740820632159
1107	3	-0.09643665188083617
1107	4	-0.027222079731537008
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2216	2	0.07041908371654415
2216	3	0.06114720009834684
2216	4	0.0374399710610831
2216	5	0.005394550843433876
2216	6	0.041455790882430676
2216	7	0.008972111391500448
2216	8	0.011092880378903658
2216	9	0.07534273550866999
2216	10-11	0.020328268705050334
2216	12-13	0.007859293230168873
2216	14-15	0.010044720252494699
2216	16-17	0.01977168373978344
2216	18-19	0.03422103258744613
2216	20-21	0.05508149413539343
2216	22-23	0.020596227418621993
2216	24-25	0.02893977940505721
2216	26-27	0.03189535055514625
2216	28-29	0.02494049570940149
2216	30-31	-0.03220072959804554
2216	32-33	-0.010254641744950277
2216	34-35	-0.05384874447820209
2216	36-37	-0.015155319883454865
2216	38-39	0.0065834209787851705
2216	40-41	0.025416804046891173
2216	42-43	-0.01121274214408885
2216	44-45	0.016192778819245746
2216	46-47	0.021486213113561803
2216	48-49	0.06319464576039735
2216	50-51	0.0678818879822316
2216	52-53	0.0542126988393008
2216	54-55	0.036720007186467285
2216	56-57	0.03022591214390502
2216	58-59	-0.006816913483525866
2216	60-61	-0.04745233314941544
2216	62-63	0.015023933226643749
2216	64-65	0.0036748666950998654
2216	66-67	0.011785576979974621
2216	68-69	0.03126267182116038
2216	70-71	-0.008035397608594508
2216	72-73	-0.008343344672077535
2216	74-75	-0.02817075851708495
2216	76-77	-0.013677324047385753
2216	78-79	0.025863045413501595
2216	80-81	0.024920357029159845
2216	82-83	0.021808872719347505
2216	84-85	0.04989955380262501
2216	86-87	0.09412917412190325
2216	88-89	0.06845845027005026
2216	90-91	-0.030077809018621338
2216	92-93	-0.00582339982631197
2216	94-95	0.005682395059544376
2216	96-97	0.01569886368150719
2216	98-99	0.07441748888901856
2216	100	0.10120193758494622
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3.0
3	4.0
4	8.0
5	27.0
6	67.0
7	166.0
8	299.0
9	644.0
10	887.0
11	1180.0
12	1603.0
13	2443.0
14	2681.0
15	3720.0
16	5013.0
17	7730.0
18	9488.0
19	5670.0
20	4077.0
21	4087.0
22	4217.0
23	4534.0
24	4891.0
25	5665.0
26	6816.0
27	8683.0
28	10894.0
29	14203.0
30	17753.0
31	23380.0
32	30400.0
33	40400.0
34	58837.0
35	104054.0
36	238057.0
37	621369.0
38	1010769.0
39	447839.0
40	2348.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.44169918252374	18.66539283812384	14.561965174525751	25.330942804826662
2	14.644131810865147	19.014450040038362	39.04305731881256	27.298360830283936
3	17.687342924301248	23.593574046565756	29.164673374971255	29.554409654161734
4	12.878488198850532	15.897188294158838	35.59376924743411	35.630554259556526
5	14.760971962174436	36.24692457408148	33.68011576507796	15.311987698666135
6	33.90172031958827	34.9463347543008	16.521427277399223	14.630517648711702
7	29.695375735792666	30.784729672676242	20.40022832586419	19.119666265666904
8	27.178134840914993	34.44178836528885	18.859176622034184	19.520900171761976
9	26.258788121916183	15.936952908792096	19.560977503517098	38.24328146577462
10-11	25.80401696549136	25.604120666086853	26.767120071795564	21.82474229662622
12-13	26.155067865574626	22.703617057302548	27.27386829708685	23.867446780035976
14-15	23.347946287227725	24.335097042189265	24.661651828196618	27.655304842386393
16-17	23.417560536299597	27.59772428320984	25.15945101234572	23.825264168144844
18-19	23.538285618797843	26.520441006083022	27.185362448824467	22.755910926294668
20-21	24.20069311096208	25.553161551639874	27.168263887913298	23.077881449484753
22-23	24.029356288166763	25.34308031406637	27.145656817649115	23.481906580117755
24-25	23.844562435811078	25.456171120179405	27.02018110795717	23.679085336052346
26-27	23.658571436229472	25.636311508849385	27.090706126332325	23.614410928588818
28-29	23.607770473354712	25.635770707004234	27.138798908353934	23.61765991128712
30-31	23.698203763718734	25.68603833518554	27.001328710574008	23.614429190521715
32-33	23.659688697597762	25.60577235189253	27.112393313749077	23.622145636760628
34-35	23.75517696174617	25.514489023131233	27.13619857351078	23.59413544161182
36-37	23.913966536832206	25.62084208226357	26.888858531946912	23.57633284895731
38-39	23.7910899290197	25.67817988465771	26.861030819745828	23.66969936657676
40-41	23.747936835034235	25.56660901173081	27.001457178131936	23.683996975103014
42-43	23.808694610565915	25.672477714089663	26.85333889371972	23.665488781624695
44-45	23.746791639824426	25.81284157424772	26.693981823374514	23.74638496255334
46-47	23.97768410374108	25.736906666745536	26.532836110034868	23.752573119478516
48-49	24.04871954889553	25.836929372218865	26.251909548407514	23.862441530478094
50-51	23.927886414151796	26.01081615058022	26.206557568569572	23.854739866698413
52-53	23.920583534008337	26.00322866931169	26.17270330717703	23.903484489502944
54-55	23.990833607837093	26.055305205663394	26.216402865782268	23.73745832071725
56-57	23.842908125106522	25.936618601385725	26.3450126679244	23.875460605583353
58-59	23.89132534463397	25.75631848199308	26.407571549385594	23.944784623987356
60-61	23.963945878520214	25.874327788536117	26.30469840044542	23.857027932498248
62-63	23.79286912683442	25.95517158002693	26.426999092724717	23.82496020041393
64-65	23.837805033372998	25.7978528223738	26.440153852040805	23.9241882922124
66-67	23.80802824581997	25.823907276810885	26.411302025084805	23.956762452284345
68-69	23.845303499730107	25.93560289561443	26.27026967073108	23.948823933924384
70-71	23.91041563953296	25.743459942755955	26.27508309037982	24.07104132733127
72-73	23.875324110693125	25.906858345858435	26.281899729065923	23.93591781438252
74-75	23.924991020757346	25.968505826294585	26.109733646918247	23.996769506029818
76-77	23.92424549950478	25.95726447380631	26.037416229769356	24.081073796919554
78-79	23.982542072673215	25.90397881180952	26.18800008724897	23.925479028268295
80-81	23.822787187429064	25.93504173527657	26.221465736702125	24.02070534059224
82-83	23.892232614884097	25.919313098450957	26.194850086879367	23.993604199785572
84-85	23.977213218300186	25.941684013262588	25.983977605099483	24.09712516333774
86-87	23.926300077175114	26.072988248124922	26.0431348808407	23.957576793859264
88-89	23.781053400554626	25.986146607430893	26.11829581680929	24.11450417520519
90-91	23.859903633468534	25.896924855474236	26.12394038776567	24.11923112329156
92-93	23.858553840397914	26.078098208363926	26.067894511520194	23.995453439717966
94-95	23.87792275778644	25.88785364602088	26.156089665620907	24.07813393057177
96-97	23.893852094620698	25.974978788534166	26.050582183108094	24.080586933737045
98-99	23.74905148901206	26.108198184958948	26.06063630876956	24.082114017259435
100	23.9861347719099	25.8294219075336	25.98732076944918	24.197122551107324
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	205.0
1	158.5
2	209.0
3	289.0
4	183.0
5	124.5
6	364.5
7	967.5
8	1685.5
9	1904.0
10	1402.0
11	891.0
12	836.0
13	951.0
14	1079.5
15	1239.0
16	1360.0
17	1516.0
18	1738.5
19	1922.0
20	2128.5
21	2465.5
22	3026.0
23	3842.5
24	4894.5
25	6348.0
26	8418.0
27	11126.0
28	14800.0
29	19111.5
30	23256.5
31	27791.0
32	32785.0
33	37339.0
34	41923.0
35	46596.0
36	51879.5
37	58007.5
38	64261.0
39	69258.0
40	73940.0
41	79584.5
42	82987.5
43	85095.0
44	88266.5
45	90581.5
46	91858.0
47	91485.5
48	91251.5
49	93012.0
50	94105.0
51	94909.5
52	94387.5
53	94667.0
54	95174.5
55	91729.5
56	87301.0
57	81479.0
58	75359.5
59	71746.0
60	66945.0
61	59417.0
62	52521.5
63	45812.0
64	38533.0
65	32662.0
66	26526.5
67	20849.5
68	16418.0
69	12539.5
70	9537.5
71	6948.5
72	5163.5
73	3917.0
74	2997.0
75	2290.0
76	1544.0
77	1100.0
78	853.5
79	579.5
80	338.5
81	139.5
82	67.0
83	27.5
84	23.0
85	18.0
86	9.5
87	7.0
88	7.0
89	6.5
90	3.5
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.23952773220215418
2	0.0
3	0.0
4	0.0
5	0.0299086178965184
6	0.006506695611603509
7	0.0
8	0.0
9	0.009649133833116566
10-11	1.2939451500347887E-4
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.8484930714782697E-5
28-29	0.0
30-31	0.001035156120027831
32-33	4.4363833715478466E-4
34-35	4.990931292991327E-4
36-37	0.0019039478636226174
38-39	1.8484930714782697E-5
40-41	7.578821593060905E-4
42-43	0.002144251962914793
44-45	0.0021627368936295755
46-47	6.65457505732177E-4
48-49	0.0028836491915061003
50-51	0.0022921314086330542
52-53	0.0028281943993617525
54-55	9.242465357391347E-4
56-57	0.0015897040414713118
58-59	0.0016081889721860944
60-61	0.001035156120027831
62-63	0.0038448655886748006
64-65	0.0036785012122417565
66-67	0.0029945587757947965
68-69	0.004658202540125239
70-71	0.006303361373740899
72-73	1.6636437643304425E-4
74-75	0.0020887971707704445
76-77	0.0013678848728939192
78-79	1.1090958428869617E-4
80-81	0.0
82-83	2.2181916857739233E-4
84-85	1.6636437643304425E-4
86-87	6.839424364469596E-4
88-89	3.327287528660885E-4
90-91	9.242465357391347E-4
92-93	1.4787944571826157E-4
94-95	4.4363833715478466E-4
96-97	3.696986142956539E-5
98-99	3.142438221513058E-4
100	3.696986142956539E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2704906.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.24224547181595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.3364272674622	47.19162280831514
2	12.24623722110336	14.754816575595958
3	3.90697185736742	7.060942730490144
4	1.7327598595466973	4.175413792100859
5	0.9887480926030529	2.978220265219146
6	0.5993372188715971	2.1663251915794937
7	0.4115162350167823	1.7353463431862952
8	0.30882332996950657	1.4883368681157305
9	0.21755831271322865	1.1795581151013974
>10	1.1699285144312126	12.442249249483096
>50	0.061539659152518876	2.4640188865557593
>100	0.019723197442804014	1.8436981058103616
>500	1.8395756556031985E-4	0.07243944995243588
>1k	2.4527675407819534E-4	0.4470116184942628
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4864	0.17982140599340607	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3782	0.1398200159266163	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.6969861429565386E-5	0.0
4	0.0	0.0	0.0	3.6969861429565386E-5	0.0
5	0.0	0.0	0.0	3.6969861429565386E-5	0.0
6	0.0	0.0	0.0	3.6969861429565386E-5	0.0
7	0.0	0.0	0.0	3.6969861429565386E-5	0.0
8	0.0	0.0	0.0	3.6969861429565386E-5	0.0
9	0.0	0.0	0.0	3.6969861429565386E-5	0.0
10-11	0.0	0.0	0.0	7.393972285913077E-5	0.0
12-13	0.0	0.0	0.0	7.393972285913077E-5	0.0
14-15	0.0	0.0	0.0	7.393972285913077E-5	0.0
16-17	0.0	0.0	0.0	7.393972285913077E-5	0.0
18-19	0.0	0.0	0.0	7.393972285913077E-5	0.0
20-21	0.0	0.0	0.0	7.393972285913077E-5	0.0
22-23	0.0	0.0	0.0	1.1090958428869617E-4	0.0
24-25	0.0	0.0	0.0	1.4787944571826155E-4	0.0
26-27	0.0	0.0	0.0	4.066684757252193E-4	0.0
28-29	0.0	0.0	0.0	0.0010536410507426136	0.0
30-31	0.0	0.0	0.0	0.002680314953643491	0.0
32-33	0.0	0.0	0.0	0.007209122978765251	0.0
34-35	0.0	3.6969861429565386E-5	0.0	0.013401574768217454	0.0
36-37	0.0	1.4787944571826155E-4	0.0	0.02257010040274967	0.0
38-39	0.0	2.2181916857739233E-4	0.0	0.03397530265377059	0.0
40-41	0.0	2.2181916857739233E-4	0.0	0.04488141177549238	0.0
42-43	0.0	2.5878903000695774E-4	0.0	0.05648994826437592	0.0
44-45	0.0	2.5878903000695774E-4	0.0	0.07035364630046294	0.0
46-47	0.0	2.957588914365231E-4	0.0	0.08732281269663345	0.0
48-49	0.0	2.957588914365231E-4	0.0	0.10320136818063178	0.0
50-51	0.0	3.1424382215130577E-4	0.0	0.11839598122818315	0.0
52-53	0.0	3.327287528660885E-4	0.0	0.13237058884855887	0.0
54-55	0.0	3.5121368358087123E-4	0.0	0.1489146018382894	0.0
56-57	0.0	3.696986142956539E-4	0.0	0.16730710789949815	0.0
58-59	0.0	4.066684757252193E-4	0.0	0.1869381043185974	0.0
60-61	0.0	4.066684757252193E-4	0.0	0.20806638012559403	0.0
62-63	0.0	4.066684757252193E-4	0.0	0.2279376806439854	0.0
64-65	0.0	4.066684757252193E-4	0.0	0.2482341345688168	0.0
66-67	0.0	4.4363833715478466E-4	0.0	0.2695472596829612	0.0
68-69	0.0	4.4363833715478466E-4	0.0	0.29391039836504484	0.0
70-71	0.0	4.4363833715478466E-4	0.0	0.31888353976071626	0.0
72-73	0.0	4.4363833715478466E-4	0.0	0.3443188044242572	0.0
74-75	3.6969861429565386E-5	4.621232678695674E-4	0.0	0.3687743677599148	0.0
76-77	3.6969861429565386E-5	4.8060819858435007E-4	0.0	0.39535569812777227	0.0
78-79	3.6969861429565386E-5	4.8060819858435007E-4	0.0	0.4263179570750333	0.0
80-81	3.6969861429565386E-5	4.8060819858435007E-4	0.0	0.4587959803409065	0.0
82-83	3.6969861429565386E-5	4.8060819858435007E-4	0.0	0.48925914615886834	0.0
84-85	3.6969861429565386E-5	4.8060819858435007E-4	0.0	0.5210162571268651	0.0
86-87	3.6969861429565386E-5	4.8060819858435007E-4	0.0	0.5545848913049104	0.0
88	3.6969861429565386E-5	4.8060819858435007E-4	0.0	0.5845304790628584	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3100	0.0	50.310047	1
GTATCAA	5935	0.0	43.50591	1
ATCAACG	7450	0.0	34.396324	3
TCAACGC	7475	0.0	34.218384	4
TATCAAC	7710	0.0	33.17112	2
CAACGCA	7860	0.0	32.482475	5
AACGCAG	8210	0.0	31.202442	6
ACGCAGA	9700	0.0	26.40754	7
CGCAGAG	9995	0.0	25.5811	8
GTACATG	9700	0.0	24.967772	1
TACATGG	9925	0.0	23.636631	2
GCAGAGT	11410	0.0	22.326302	9
ACATGGG	10200	0.0	22.310894	3
AGAGTAC	10195	0.0	20.374268	10-11
TACCGTC	380	0.0	19.789715	7
CGTATAC	145	6.5852836E-4	19.456066	3
TATAACG	170	8.614961E-5	19.358189	2
CATGGGG	8870	0.0	19.083176	4
CAGAGTA	10660	0.0	18.84629	10-11
ATACCGT	475	0.0	18.80162	6
>>END_MODULE