FastQCFastQC Report
Wed 25 May 2016
SRR2049391_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049391_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173318
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28100.036.8210561
GGTATCA18500.033.1993871
TCAACGC31350.033.1607364
ATCAACG31450.032.457763
CAACGCA35500.029.5430765
AACGCAG37100.029.1106576
TATCAAC36250.028.8094122
ACGCAGA43950.024.568977
GTACATG79200.024.2191731
TACATGG81400.023.0010742
CGCAGAG47250.022.9518768
ACATGGG82400.022.0934333
GTATACG1150.003842150320.5414281
GTACCTG18400.020.284661
TACCTGG18100.020.2724152
TACACCG3052.382876E-1020.0457695
CATGGGG70950.019.2271544
GCAGAGT56900.018.5642879
GAGTACA53700.017.5080281
AGAGTAC46500.017.21331210-11
Produced by FastQC (version 0.11.5)