FastQCFastQC Report
Wed 25 May 2016
SRR2049391_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049391_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173318
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42620.19610567804619483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33040.15202561245064No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24540.11291490706836274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28150.047.209071
GTATCAA49300.042.059151
TCAACGC61200.032.6528744
ATCAACG62400.032.0249373
CAACGCA65250.030.770275
TATCAAC65300.030.7445832
AACGCAG69150.028.8875526
ACGCAGA80250.024.8901757
CGCAGAG83400.023.9500778
GTACATG75650.022.9864651
TATCACG3251.8189894E-1221.7001062
TATACCG1754.4247736E-621.4949235
GTACCTG17700.021.2995991
TACATGG77500.021.2334372
GCAGAGT94450.021.0485639
AGAGTAC83600.020.49015810-11
ACATGGG79750.020.1051653
GTGGTAT15100.019.9736631
TACCTGG18400.018.6534422
GAGTACT51750.018.34404412-13
Produced by FastQC (version 0.11.5)