##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049391_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2173318
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.03206157589455	34.0	33.0	34.0	31.0	34.0
2	33.1701748202518	34.0	33.0	34.0	31.0	34.0
3	33.26436490196097	34.0	34.0	34.0	31.0	34.0
4	36.57460850183912	37.0	37.0	37.0	35.0	37.0
5	36.53549549582712	37.0	37.0	37.0	35.0	37.0
6	36.558837224925206	37.0	37.0	37.0	35.0	37.0
7	36.535707153762125	37.0	37.0	37.0	35.0	37.0
8	36.54128434034964	37.0	37.0	37.0	35.0	37.0
9	38.364301956731595	39.0	39.0	39.0	37.0	39.0
10-11	38.37203966469702	39.0	39.0	39.0	37.0	39.0
12-13	38.36390624841832	39.0	39.0	39.0	37.0	39.0
14-15	40.01077384901795	41.0	40.0	41.0	38.0	41.0
16-17	40.01936278998288	41.0	40.0	41.0	38.0	41.0
18-19	40.01928410844616	41.0	40.0	41.0	38.0	41.0
20-21	40.00733118669242	41.0	40.0	41.0	38.0	41.0
22-23	39.97658327037277	41.0	40.0	41.0	38.0	41.0
24-25	39.94878200060921	41.0	40.0	41.0	38.0	41.0
26-27	39.88234257480958	41.0	40.0	41.0	38.0	41.0
28-29	39.81773629077751	41.0	40.0	41.0	38.0	41.0
30-31	39.738150606584036	41.0	40.0	41.0	37.0	41.0
32-33	39.64383376017683	41.0	40.0	41.0	37.0	41.0
34-35	39.56818882464508	41.0	40.0	41.0	36.0	41.0
36-37	39.4820916681314	41.0	40.0	41.0	36.0	41.0
38-39	39.39925036280931	41.0	40.0	41.0	35.5	41.0
40-41	39.29722640681207	41.0	39.5	41.0	35.0	41.0
42-43	39.18653505837618	41.0	39.0	41.0	35.0	41.0
44-45	39.051188091204324	41.0	39.0	41.0	35.0	41.0
46-47	38.8589329771345	41.0	39.0	41.0	35.0	41.0
48-49	38.66048203714321	41.0	39.0	41.0	35.0	41.0
50-51	38.48915000013804	41.0	38.0	41.0	35.0	41.0
52-53	38.34710037831555	40.0	38.0	41.0	35.0	41.0
54-55	38.2355465237945	40.0	37.0	41.0	35.0	41.0
56-57	38.08177082230948	40.0	37.0	41.0	35.0	41.0
58-59	37.864086157663074	40.0	36.5	41.0	35.0	41.0
60-61	37.62027807251401	39.5	36.0	41.0	34.0	41.0
62-63	37.354080028785475	39.0	35.0	41.0	34.0	41.0
64-65	37.06462998051827	39.0	35.0	41.0	34.0	41.0
66-67	36.754916215666555	37.5	35.0	41.0	34.0	41.0
68-69	36.431563397533175	37.0	35.0	40.5	33.5	41.0
70-71	36.096921849448634	37.0	35.0	39.5	33.0	41.0
72-73	35.75934285732691	36.0	35.0	39.0	33.0	41.0
74-75	35.415906461916755	36.0	35.0	39.0	33.0	41.0
76-77	35.10211091980097	35.0	35.0	37.0	33.0	39.5
78-79	34.791666474947526	35.0	35.0	37.0	33.0	39.0
80-81	34.50969922487183	35.0	35.0	36.5	33.0	39.0
82-83	34.26295553618937	35.0	35.0	36.0	32.5	38.0
84-85	34.03413168252414	35.0	35.0	36.0	32.0	37.0
86-87	33.827415270107736	35.0	35.0	35.5	32.0	37.0
88-89	33.67884336300532	35.0	35.0	35.0	32.0	36.0
90-91	33.55339301473599	35.0	35.0	35.0	32.0	36.0
92-93	33.43813077515578	35.0	35.0	35.0	32.0	36.0
94-95	33.34552536720351	35.0	35.0	35.0	32.0	36.0
96-97	33.320640835809584	35.0	35.0	35.0	32.0	36.0
98-99	33.26348422090095	35.0	35.0	35.0	32.0	35.0
100	33.210859616494226	35.0	35.0	35.0	32.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.09579079982391647
1101	2	-0.05371141777349209
1101	3	-0.06350763184199337
1101	4	-0.020591590241274105
1101	5	0.02716025556102153
1101	6	-0.034685459954950204
1101	7	0.034204813144683044
1101	8	3.372258913714177E-4
1101	9	0.02407832243119401
1101	10-11	0.062127008567820496
1101	12-13	0.012881481724598132
1101	14-15	0.039100241768082356
1101	16-17	-0.026970122178866518
1101	18-19	-0.03871737199166603
1101	20-21	0.0034076212646567683
1101	22-23	-0.02662380193989833
1101	24-25	-0.0340387586859876
1101	26-27	0.06263480926648413
1101	28-29	0.02340069843168635
1101	30-31	-0.051826910894455125
1101	32-33	0.0928079931469199
1101	34-35	-0.047235871358772386
1101	36-37	-0.016911328138597526
1101	38-39	0.06412279341218863
1101	40-41	0.03921756271215315
1101	42-43	0.1396604220310067
1101	44-45	0.10342522979143354
1101	46-47	0.20753946563277736
1101	48-49	0.2546743544078254
1101	50-51	0.14311592495099035
1101	52-53	0.21351633780918888
1101	54-55	0.21132939427905484
1101	56-57	0.25180770856785983
1101	58-59	0.25766479566065215
1101	60-61	0.1855514721248568
1101	62-63	0.17952673381491735
1101	64-65	0.12206702107857126
1101	66-67	0.13179834981654182
1101	68-69	0.20327046777376978
1101	70-71	0.18248270959949053
1101	72-73	0.14203251918775095
1101	74-75	0.11877014227232507
1101	76-77	0.11963849544574856
1101	78-79	0.12698673099017554
1101	80-81	0.1349517260651254
1101	82-83	0.13476308115368596
1101	84-85	0.15743166912115214
1101	86-87	0.13117066893278206
1101	88-89	0.08413848317179884
1101	90-91	0.13054944174223237
1101	92-93	0.15879709598629432
1101	94-95	0.20348453581634374
1101	96-97	0.19247906558921102
1101	98-99	0.19763686295086558
1101	100	0.24526552021868753
1102	1	-0.09582116173793764
1102	2	-0.01343127851351511
1102	3	-0.11710045833377336
1102	4	-0.049822016927372204
1102	5	-0.11342350283911173
1102	6	-0.17250628417971114
1102	7	-0.05611985692251409
1102	8	-0.1152141464671459
1102	9	0.0030051299771400863
1102	10-11	-0.009164789667138962
1102	12-13	-0.04436185889559141
1102	14-15	-0.01669584761739884
1102	16-17	-0.024015907945198478
1102	18-19	-0.051335783456323725
1102	20-21	-0.007705851328651647
1102	22-23	0.004224914763199195
1102	24-25	-0.03641710861717229
1102	26-27	-0.01567969968016314
1102	28-29	0.053827384529974154
1102	30-31	0.04298423800036488
1102	32-33	0.036887419913981034
1102	34-35	0.0031568093958185273
1102	36-37	-0.02343913965183475
1102	38-39	0.03218610812354683
1102	40-41	0.09206854643817053
1102	42-43	0.010815591652900025
1102	44-45	0.021029067537362778
1102	46-47	0.09681462553886178
1102	48-49	0.04981341197401434
1102	50-51	0.04360272763903339
1102	52-53	0.12490914001143238
1102	54-55	0.07262301422885997
1102	56-57	0.04640227580937761
1102	58-59	0.15059451795035272
1102	60-61	0.07562009005052772
1102	62-63	0.025056448008093923
1102	64-65	0.07155491679551318
1102	66-67	0.06015030512380548
1102	68-69	0.052543805965008517
1102	70-71	0.07900222218956543
1102	72-73	0.08979687025244232
1102	74-75	0.03537507304716314
1102	76-77	0.012795932090547524
1102	78-79	0.0463869819819962
1102	80-81	0.09986347410172414
1102	82-83	0.05297820543512444
1102	84-85	0.03390523401468215
1102	86-87	0.052220583919421415
1102	88-89	0.015634920707221056
1102	90-91	0.042044462388332704
1102	92-93	-0.03774771819879419
1102	94-95	0.01917051266032388
1102	96-97	0.0262172244427461
1102	98-99	0.13023138970982728
1102	100	0.15164759454465582
1103	1	-0.07341449476604822
1103	2	-0.0741751957791692
1103	3	-0.07144612246204218
1103	4	-0.05222776824616204
1103	5	-0.05633024357193506
1103	6	-0.051576616510914164
1103	7	0.007643172057903769
1103	8	-0.010713075661726634
1103	9	-0.009536137998914285
1103	10-11	-0.04831417338174049
1103	12-13	-0.027589447014484847
1103	14-15	-0.05894605236760242
1103	16-17	-0.02843170855665278
1103	18-19	-0.045714436530630564
1103	20-21	-0.036804861405926204
1103	22-23	-0.06018943606419214
1103	24-25	-0.008949423540784096
1103	26-27	-0.07308663371752999
1103	28-29	-0.08312633938027147
1103	30-31	-0.117815826604037
1103	32-33	-0.061877338633379964
1103	34-35	-0.08313738994185371
1103	36-37	-0.03960817429913277
1103	38-39	-0.032942007004571394
1103	40-41	-0.051565311869602226
1103	42-43	0.0059605319065383355
1103	44-45	-0.0420821944869445
1103	46-47	-0.06948635217516141
1103	48-49	-0.1684156513637447
1103	50-51	-0.15656889372824168
1103	52-53	-0.15136800528744487
1103	54-55	-0.03825551675836181
1103	56-57	-0.19732632715835052
1103	58-59	-0.23001411157132168
1103	60-61	-0.2640043522913089
1103	62-63	-0.2668751857713616
1103	64-65	-0.22613140589080416
1103	66-67	-0.2344881645146657
1103	68-69	-0.17021691353483703
1103	70-71	-0.20545882694539586
1103	72-73	-0.15441041580267267
1103	74-75	-0.15305596810127042
1103	76-77	-0.2178212816788374
1103	78-79	-0.29048452391124613
1103	80-81	-0.2519903418825251
1103	82-83	-0.2138462842154496
1103	84-85	-0.21734781180358453
1103	86-87	-0.198179823546198
1103	88-89	-0.19486679359884107
1103	90-91	-0.18507425712169123
1103	92-93	-0.1825532945261088
1103	94-95	-0.20939436644803067
1103	96-97	-0.21117957382362107
1103	98-99	-0.15916394859855387
1103	100	-0.2311084958201306
1104	1	-0.07020081495230102
1104	2	-0.009533007533867988
1104	3	0.027484390284925553
1104	4	0.002123560402488067
1104	5	0.014251374588411636
1104	6	0.001976432978473497
1104	7	-0.022588738973190914
1104	8	-0.0071897595749135235
1104	9	-3.732815620978158E-4
1104	10-11	-0.018835254976309557
1104	12-13	-0.00703387752562179
1104	14-15	-0.05862072627186876
1104	16-17	-0.006635216729684146
1104	18-19	-0.04610234362358767
1104	20-21	-0.02781221577257753
1104	22-23	-0.04635659900074529
1104	24-25	-0.01771284324223643
1104	26-27	0.012847357059001752
1104	28-29	0.049383365428788295
1104	30-31	0.05103531109234183
1104	32-33	0.05426265119437801
1104	34-35	0.035854581742299274
1104	36-37	0.08233918943624019
1104	38-39	0.12079091679269283
1104	40-41	0.05906998687947862
1104	42-43	0.08556879031601028
1104	44-45	0.11322185109816729
1104	46-47	0.09036619677322477
1104	48-49	0.0474837374106869
1104	50-51	0.03303161842561053
1104	52-53	0.005908381755276082
1104	54-55	0.006395044146849216
1104	56-57	0.021421477603674077
1104	58-59	0.025770708216199978
1104	60-61	0.04723769091823016
1104	62-63	0.050360547989939164
1104	64-65	0.09124957759244268
1104	66-67	0.10358918084340729
1104	68-69	0.0042096383045233665
1104	70-71	0.04932796559960906
1104	72-73	0.016195901942154478
1104	74-75	-0.0013886332304196003
1104	76-77	0.06195565421052862
1104	78-79	0.04886720352039475
1104	80-81	0.05204585311201981
1104	82-83	0.025452157250271057
1104	84-85	0.020571827064244985
1104	86-87	0.05270222381400913
1104	88-89	0.03868768699255298
1104	90-91	0.04216310861889383
1104	92-93	0.0020942258816560866
1104	94-95	-0.026220381885622146
1104	96-97	-0.008821571770390335
1104	98-99	0.0015922509317718436
1104	100	0.05465571205718334
1105	1	-0.10329775186110624
1105	2	-0.11175081847543566
1105	3	-0.09248414718639708
1105	4	-0.013603731967975818
1105	5	-0.0698519910078943
1105	6	-0.04843102288274537
1105	7	-0.06754018563215425
1105	8	0.009338054644096871
1105	9	-0.043367796160325156
1105	10-11	-0.02471098774772429
1105	12-13	-0.06472796605648767
1105	14-15	-0.0706279298336625
1105	16-17	-0.07049811255335214
1105	18-19	0.005913021765728388
1105	20-21	0.010050685952300853
1105	22-23	-0.04923508445970981
1105	24-25	-0.01974121399075557
1105	26-27	-0.09676092515321244
1105	28-29	-0.04068635055958225
1105	30-31	-0.013817739697110198
1105	32-33	-0.032899754193330466
1105	34-35	0.016895298730247532
1105	36-37	-0.11479788387222101
1105	38-39	-0.09760611707752531
1105	40-41	-0.07260439524112883
1105	42-43	-0.07552459951157431
1105	44-45	-0.05575532846838627
1105	46-47	-0.05597513239717955
1105	48-49	-0.02740761223238053
1105	50-51	0.002873427409788576
1105	52-53	-0.0052021783240334685
1105	54-55	0.026221918530062283
1105	56-57	0.01174837286809094
1105	58-59	-0.023810521487035885
1105	60-61	0.034973841459006394
1105	62-63	-0.013773349559606629
1105	64-65	0.012160368091315377
1105	66-67	0.06691974212112228
1105	68-69	0.08903601562425933
1105	70-71	0.07419299811527225
1105	72-73	0.09234330146043135
1105	74-75	0.05454785930818673
1105	76-77	-0.029596343621456356
1105	78-79	0.004535270438303485
1105	80-81	-0.020616768350777193
1105	82-83	0.0020568665067344227
1105	84-85	-0.07045910526339583
1105	86-87	-0.011303719612456575
1105	88-89	-0.0068507031028559595
1105	90-91	-0.021730938785083254
1105	92-93	-0.04386439846455659
1105	94-95	-0.06604095332249926
1105	96-97	-0.08366446339966416
1105	98-99	-0.11425854695722393
1105	100	-0.17098227684672906
1106	1	-0.06708527291677768
1106	2	-0.06478344722863483
1106	3	-0.07230227949688128
1106	4	-0.07154354530566565
1106	5	-0.0308693853755031
1106	6	-0.06976969600167848
1106	7	-0.049108331710229436
1106	8	-0.019365514702165854
1106	9	-0.037718822523295614
1106	10-11	-0.05814847764056452
1106	12-13	-0.0608444127767811
1106	14-15	-0.09243082207669318
1106	16-17	-0.11197745985590757
1106	18-19	-0.1321391460397905
1106	20-21	-0.07483672793469509
1106	22-23	-0.09721213366726289
1106	24-25	-0.06722011679681117
1106	26-27	-0.059773855324863234
1106	28-29	-0.16410733901142294
1106	30-31	-0.11916832811956368
1106	32-33	-0.07803073643007963
1106	34-35	-0.13732603037112057
1106	36-37	-0.11371559825983013
1106	38-39	-0.11442295610860498
1106	40-41	-0.13247964775622734
1106	42-43	-0.18913071616808708
1106	44-45	-0.15290961115068313
1106	46-47	-0.15128854613907095
1106	48-49	-0.16906432107012392
1106	50-51	-0.15221394871467453
1106	52-53	-0.14098356777552823
1106	54-55	-0.10815288205840545
1106	56-57	-0.1810150535924322
1106	58-59	-0.11876313693593943
1106	60-61	-0.0783170410741576
1106	62-63	-0.10805118915799738
1106	64-65	-0.1602649940837395
1106	66-67	-0.11079035350025634
1106	68-69	-0.10600736758417639
1106	70-71	-0.025227528357397944
1106	72-73	-0.02138923664519865
1106	74-75	-0.04299961921218909
1106	76-77	-0.054148055566749065
1106	78-79	-0.019657883179156954
1106	80-81	-0.08165188575991777
1106	82-83	-0.08825585229962485
1106	84-85	-0.015572965424887286
1106	86-87	-0.01824854041961288
1106	88-89	-0.11286912894711776
1106	90-91	-0.15627838059187127
1106	92-93	-0.13011988474023184
1106	94-95	-0.04903349871840135
1106	96-97	-0.15543174565974738
1106	98-99	-0.0785024199422395
1106	100	-0.04626910165566045
1107	1	-0.15768301419306852
1107	2	-0.13553934071680374
1107	3	-0.12053952890415331
1107	4	-0.0629827876467175
1107	5	-0.06645013553620771
1107	6	-0.10929678662301257
1107	7	-0.1649143549664771
1107	8	-0.08675314881995178
1107	9	-0.08168733406941442
1107	10-11	-0.10305061572625362
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2216	8	0.0715862806533778
2216	9	0.1076924516706228
2216	10-11	0.044490595800979804
2216	12-13	0.026356543151983658
2216	14-15	0.040104864793548245
2216	16-17	0.05460585400847151
2216	18-19	0.04925354505152768
2216	20-21	0.112512069800772
2216	22-23	0.04339712421528219
2216	24-25	0.06658944491146457
2216	26-27	0.10260313724783288
2216	28-29	0.09204162948848449
2216	30-31	0.0844974591738108
2216	32-33	0.09187877968966518
2216	34-35	0.0328090811227284
2216	36-37	0.06330015380603982
2216	38-39	0.09062255356786153
2216	40-41	0.128145762329666
2216	42-43	0.16310504731877273
2216	44-45	0.16752692378359058
2216	46-47	0.11599245928790936
2216	48-49	0.13719399755299833
2216	50-51	0.09529348096877754
2216	52-53	0.04520490467128724
2216	54-55	0.007594525942074881
2216	56-57	0.05000814301076417
2216	58-59	0.04900463516395348
2216	60-61	0.06044406585790085
2216	62-63	0.08012707776633476
2216	64-65	0.04698611289600052
2216	66-67	0.053710485101305494
2216	68-69	0.04854281152410067
2216	70-71	0.055593123870330885
2216	72-73	0.05874076585244836
2216	74-75	0.05163886887702063
2216	76-77	0.04478004548941783
2216	78-79	0.07121967952523534
2216	80-81	0.06387653225206691
2216	82-83	0.025417112528259622
2216	84-85	0.05435851974526429
2216	86-87	0.09055444707783522
2216	88-89	0.09134386741497025
2216	90-91	0.0030000222911681362
2216	92-93	0.006240397417194288
2216	94-95	0.025092938118014274
2216	96-97	0.07449325515629823
2216	98-99	0.08008507647723917
2216	100	0.09950343321699506
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	5.0
4	8.0
5	20.0
6	52.0
7	155.0
8	286.0
9	506.0
10	770.0
11	1051.0
12	1619.0
13	2206.0
14	2376.0
15	3094.0
16	4166.0
17	6250.0
18	7736.0
19	4699.0
20	3353.0
21	3393.0
22	3628.0
23	3759.0
24	4150.0
25	4621.0
26	5738.0
27	7283.0
28	8977.0
29	11477.0
30	14545.0
31	18865.0
32	24885.0
33	32976.0
34	47884.0
35	84247.0
36	186078.0
37	483721.0
38	810494.0
39	376076.0
40	2168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.71435687403144	18.618921112833	14.237602390967458	25.429119622168106
2	14.530639326596475	19.10539552886416	39.395523342649355	26.968441801890012
3	17.6005996361324	24.13760894632079	29.280160565550002	28.981630851996808
4	12.537235692153656	16.035435219328235	35.93095902210353	35.49637006641458
5	14.45106259979205	36.23393258809662	33.95145912144793	15.363545690663399
6	33.468035654547904	35.21408880137788	16.832513722933186	14.485361821141025
7	29.325482971198873	30.783115954499063	20.82313770925378	19.06826336504828
8	26.863026947736135	34.61191597364031	19.186607758275596	19.338449320347966
9	26.150316276578415	15.932553432933075	19.80619373815631	38.110936552332205
10-11	25.555810159047425	25.617559174606154	27.19602413641103	21.63060652993539
12-13	25.972545205073533	22.865981876559253	27.61533286891288	23.546140049454337
14-15	23.15604527271205	24.49319427713754	25.017530798530174	27.33322965162024
16-17	23.170999365946447	27.76100874331322	25.45787132854005	23.610120562200283
18-19	23.290494074037944	26.650632811213086	27.53073871380074	22.528134400948225
20-21	23.95910308569662	25.75173076374465	27.484956182206194	22.804209968352538
22-23	23.761594023516118	25.520586494935394	27.491374939148344	23.226444542400145
24-25	23.652889268850668	25.568784687744728	27.392309823044762	23.38601622035984
26-27	23.4125478674883	25.791583604328405	27.410721167063716	23.38514736111958
28-29	23.395172726678744	25.78285828396949	27.478376381183057	23.34359260816871
30-31	23.470538081081415	25.84761769790323	27.299168038238676	23.382676182776677
32-33	23.432649228063667	25.779885068842283	27.448820941047742	23.338644762046307
34-35	23.5357642590695	25.695037180767873	27.398436755884255	23.370761804278377
36-37	23.691071350513162	25.758885742090893	27.16542008986396	23.384622817531987
38-39	23.53070282397698	25.897728726306962	27.176671798604712	23.394896651111342
40-41	23.54237660919027	25.78302653386696	27.281003341465365	23.3935935154774
42-43	23.543675918427446	25.818900463815066	27.1609732754178	23.47645034233969
44-45	23.468006643440546	25.95904377340797	27.057042803901858	23.515906779249622
46-47	23.746626393126228	25.910290067498593	26.85475045029355	23.488333089081625
48-49	23.75505089953924	26.02825765908824	26.593953806082727	23.622737635289795
50-51	23.651224503036698	26.14037783981495	26.583332144636902	23.62506551251145
52-53	23.668761438709225	26.218424561173443	26.491078525768224	23.62173547434911
54-55	23.71842123668382	26.2516702740906	26.53798654210011	23.491921947125473
56-57	23.55805643262126	26.145249443008318	26.609892319542517	23.686801804827905
58-59	23.56008234542747	25.961356178023674	26.730675788346115	23.74788568820274
60-61	23.685076296380483	26.117942245459787	26.5962469073543	23.600734550805434
62-63	23.530356406631917	26.194571443295935	26.71324211502783	23.56183003504432
64-65	23.58696089810915	26.0086069944196	26.677518397134225	23.726913710337023
66-67	23.51244344021533	26.066310257869134	26.709562414140827	23.71168388777471
68-69	23.597275641062513	26.099474578716215	26.596182556804838	23.707067223416438
70-71	23.577287025601287	25.98880806489316	26.55457092213594	23.879333987369613
72-73	23.579810616108468	26.160596932651824	26.53569289800054	23.72389955323917
74-75	23.64463271194934	26.157784570418634	26.453744523797585	23.743838193834442
76-77	23.602668953833046	26.141037120747107	26.37945488996846	23.876839035451386
78-79	23.722257536929174	26.17795253381297	26.44040867513253	23.65938125412532
80-81	23.54683023837285	26.17237790327969	26.48781724533639	23.792974613011072
82-83	23.577971414603287	26.128985132142756	26.51841371178788	23.77462974146608
84-85	23.666915211765254	26.080693797423244	26.35886338585603	23.893527604955473
86-87	23.614574859292578	26.291190739997717	26.358553612380042	23.735680788329656
88-89	23.507106344278956	26.182456254979456	26.401017436811085	23.909419963930503
90-91	23.53953870494772	26.139761546352787	26.392189369641343	23.928510379058153
92-93	23.61262026273694	26.25671526141293	26.367812849911964	23.76285162593817
94-95	23.605121777493114	26.07998958270066	26.393865940768634	23.921022699037596
96-97	23.54169983407858	26.158090992666512	26.416980855999906	23.883228317254996
98-99	23.506081961540726	26.2610936671771	26.41588046263031	23.816943908651865
100	23.65174955298353	25.992109687672837	26.352483644672752	24.003657114670883
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	310.0
1	245.0
2	602.5
3	820.5
4	377.0
5	170.0
6	413.0
7	1094.5
8	1827.0
9	1942.5
10	1366.0
11	883.0
12	857.5
13	904.5
14	1010.5
15	1119.0
16	1237.5
17	1375.0
18	1516.5
19	1738.5
20	1963.5
21	2245.5
22	2740.0
23	3521.5
24	4584.0
25	6032.0
26	8040.0
27	10615.5
28	13498.0
29	17069.0
30	20712.5
31	24165.0
32	28173.0
33	31923.5
34	35494.0
35	39187.0
36	43553.0
37	48652.0
38	53670.5
39	57734.0
40	61141.0
41	65068.5
42	67854.5
43	69404.0
44	71657.0
45	73389.5
46	74050.5
47	73389.0
48	72692.5
49	73419.5
50	74269.5
51	74696.5
52	73592.0
53	73539.5
54	73605.5
55	70791.5
56	67701.0
57	63131.5
58	58213.5
59	55343.5
60	51435.0
61	45572.0
62	40213.0
63	35014.0
64	29571.5
65	25105.5
66	20524.5
67	16161.5
68	12596.5
69	9662.5
70	7245.5
71	5330.0
72	4017.5
73	2988.0
74	2271.0
75	1723.5
76	1217.5
77	870.0
78	641.5
79	461.5
80	276.5
81	109.5
82	47.5
83	24.5
84	14.0
85	9.0
86	7.5
87	9.5
88	6.5
89	4.0
90	4.0
91	2.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.23733296277857174
2	0.0
3	0.0
4	0.0
5	0.031058501332984868
6	0.006027649888327433
7	0.0
8	0.0
9	0.010122770804824698
10-11	1.8405037826953993E-4
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	2.300629728369249E-5
28-29	0.0
30-31	9.662644859150846E-4
32-33	7.822141076455446E-4
34-35	7.822141076455446E-4
36-37	0.0015184156207237046
38-39	0.0
40-41	9.202518913476996E-4
42-43	0.0017484785935606294
44-45	0.002139585647383402
46-47	6.211700266596973E-4
48-49	0.003059837538731101
50-51	0.0031748690251495637
52-53	0.0023236360256529416
54-55	0.0010122770804824697
56-57	0.0016564534044258593
58-59	0.0015644282152910893
60-61	7.822141076455446E-4
62-63	0.003220881619716949
64-65	0.003934076835511416
66-67	0.0029678123495963317
68-69	0.005038379105128656
70-71	0.006717838806838208
72-73	3.450944592553874E-4
74-75	0.0023466423229366344
76-77	0.0014724030261563195
78-79	1.1503148641846245E-4
80-81	0.0
82-83	2.530692701206174E-4
84-85	1.6104408098584745E-4
86-87	6.441763239433898E-4
88-89	6.211700266596973E-4
90-91	0.0010812959723335471
92-93	1.6104408098584745E-4
94-95	4.601259456738498E-4
96-97	0.0
98-99	2.7607556740430987E-4
100	9.202518913476996E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2173318.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.39798196688065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.7741063997795	51.37291465357018
2	11.888016552789075	15.311285511769793
3	3.613771067987274	6.981586922060385
4	1.5959168295759978	4.110952928467058
5	0.853372414277531	2.7477730672838927
6	0.5191908817831394	2.006090702546369
7	0.3440060797659256	1.5507308124884367
8	0.2553153759051595	1.3153435978726253
9	0.1814532207027085	1.0516699111180892
>10	0.9276753873151424	10.335808440919012
>50	0.03642993798491098	1.570232912400289
>100	0.01024518116890712	1.0220924565441158
>500	2.1457327057279287E-4	0.10446472720344516
>1k	2.8609769409705703E-4	0.5190533557564476
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4262	0.19610567804619483	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3304	0.15202561245064	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2454	0.11291490706836274	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	2.300629728369249E-5	0.0	0.0
12-13	0.0	0.0	4.601259456738498E-5	0.0	0.0
14-15	0.0	9.202518913476997E-5	4.601259456738498E-5	0.0	0.0
16-17	0.0	1.8405037826953993E-4	4.601259456738498E-5	0.0	0.0
18-19	0.0	1.8405037826953993E-4	9.202518913476997E-5	0.0	0.0
20-21	0.0	1.8405037826953993E-4	9.202518913476997E-5	0.0	0.0
22-23	0.0	1.8405037826953993E-4	9.202518913476997E-5	2.0705667555323245E-4	0.0
24-25	0.0	1.8405037826953993E-4	9.202518913476997E-5	4.1411335110646484E-4	0.0
26-27	0.0	1.8405037826953993E-4	9.202518913476997E-5	8.052204049292372E-4	0.0
28-29	0.0	1.8405037826953993E-4	9.202518913476997E-5	0.0018405037826953994	0.0
30-31	0.0	1.8405037826953993E-4	9.202518913476997E-5	0.0046012594567384985	0.0
32-33	0.0	2.0705667555323245E-4	9.202518913476997E-5	0.010329827480377928	0.0
34-35	0.0	2.3006297283692493E-4	9.202518913476997E-5	0.019808421961259235	0.0
36-37	0.0	2.3006297283692493E-4	9.202518913476997E-5	0.031794702846063026	0.0
38-39	0.0	2.3006297283692493E-4	9.202518913476997E-5	0.048175186512052076	0.0
40-41	0.0	2.3006297283692493E-4	9.202518913476997E-5	0.06338234901657282	0.0
42-43	0.0	2.3006297283692493E-4	9.202518913476997E-5	0.076910051819384	0.0
44-45	0.0	2.3006297283692493E-4	9.202518913476997E-5	0.09450986924140876	0.0
46-47	0.0	2.3006297283692493E-4	9.202518913476997E-5	0.11602075720166125	0.0
48-49	0.0	2.3006297283692493E-4	9.202518913476997E-5	0.136910475135254	0.0
50-51	0.0	2.760755674043099E-4	9.202518913476997E-5	0.1553845318540591	0.0
52-53	0.0	3.220881619716949E-4	9.202518913476997E-5	0.17365153189731092	0.0
54-55	0.0	3.220881619716949E-4	9.202518913476997E-5	0.19371302312869076	0.0
56-57	0.0	3.220881619716949E-4	9.202518913476997E-5	0.21625919446670944	0.0
58-59	0.0	3.220881619716949E-4	9.202518913476997E-5	0.24163514037062225	0.0
60-61	0.0	3.6810075653907987E-4	9.202518913476997E-5	0.26772428149032956	0.0
62-63	0.0	3.6810075653907987E-4	9.202518913476997E-5	0.2920419377191925	0.0
64-65	0.0	3.6810075653907987E-4	9.202518913476997E-5	0.3190053181356801	0.0
66-67	0.0	3.6810075653907987E-4	9.202518913476997E-5	0.34612974263315355	0.0
68-69	0.0	3.6810075653907987E-4	9.202518913476997E-5	0.37497963942690393	0.0
70-71	0.0	3.6810075653907987E-4	9.202518913476997E-5	0.4054629833277965	0.0
72-73	0.0	4.1411335110646484E-4	9.202518913476997E-5	0.4361303776069586	0.0
74-75	0.0	4.1411335110646484E-4	9.202518913476997E-5	0.4661305892648936	0.0
76-77	0.0	4.1411335110646484E-4	9.202518913476997E-5	0.49944370773168034	0.0
78-79	0.0	4.1411335110646484E-4	9.202518913476997E-5	0.5349424244404178	0.0
80-81	2.300629728369249E-5	4.1411335110646484E-4	9.202518913476997E-5	0.5743061990928158	0.0
82-83	4.601259456738498E-5	4.1411335110646484E-4	9.202518913476997E-5	0.6149353200958166	0.0
84-85	4.601259456738498E-5	4.3711964839015735E-4	9.202518913476997E-5	0.6538619750998244	0.0
86-87	4.601259456738498E-5	4.6012594567384986E-4	9.202518913476997E-5	0.6937088819951798	0.0
88	4.601259456738498E-5	4.6012594567384986E-4	9.202518913476997E-5	0.724514314058044	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2815	0.0	47.20907	1
GTATCAA	4930	0.0	42.05915	1
TCAACGC	6120	0.0	32.652874	4
ATCAACG	6240	0.0	32.024937	3
CAACGCA	6525	0.0	30.77027	5
TATCAAC	6530	0.0	30.744583	2
AACGCAG	6915	0.0	28.887552	6
ACGCAGA	8025	0.0	24.890175	7
CGCAGAG	8340	0.0	23.950077	8
GTACATG	7565	0.0	22.986465	1
TATCACG	325	1.8189894E-12	21.700106	2
TATACCG	175	4.4247736E-6	21.494923	5
GTACCTG	1770	0.0	21.299599	1
TACATGG	7750	0.0	21.233437	2
GCAGAGT	9445	0.0	21.048563	9
AGAGTAC	8360	0.0	20.490158	10-11
ACATGGG	7975	0.0	20.105165	3
GTGGTAT	1510	0.0	19.973663	1
TACCTGG	1840	0.0	18.653442	2
GAGTACT	5175	0.0	18.344044	12-13
>>END_MODULE