FastQCFastQC Report
Wed 25 May 2016
SRR2049390_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049390_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2334815
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31250.037.4830741
GGTATCA20650.035.6810871
TCAACGC34950.032.563584
ATCAACG36250.031.5255133
CAACGCA39050.029.1421075
AACGCAG40000.028.7618966
TAATACG1154.178848E-628.6262284
TATCAAC41750.027.373042
GTACATG88700.025.3996831
TACATGG89750.024.3140182
ACGCAGA48400.023.669597
ACATGGG95700.022.0647143
CGCAGAG53400.021.277038
TAGACCG1354.0643092E-420.8998955
TTAAACG1200.00504774919.5953354
TATACTG11050.018.7247925
CATGGGG80550.018.624694
GAGTACA59950.017.8054451
GCAGAGT64700.017.6335169
AGAGTAC51450.017.61106510-11
Produced by FastQC (version 0.11.5)