##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049390_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2334815
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44432299775357	33.0	31.0	34.0	30.0	34.0
2	31.684731338457222	34.0	31.0	34.0	30.0	34.0
3	31.92586307694614	34.0	31.0	34.0	30.0	34.0
4	35.38833269445331	37.0	35.0	37.0	33.0	37.0
5	35.4666827992796	37.0	37.0	37.0	35.0	37.0
6	35.512061555198166	37.0	37.0	37.0	35.0	37.0
7	35.43231091114285	37.0	37.0	37.0	35.0	37.0
8	35.45173086518632	37.0	37.0	37.0	35.0	37.0
9	37.1689234478963	39.0	39.0	39.0	35.0	39.0
10-11	37.17750506999484	39.0	39.0	39.0	35.0	39.0
12-13	37.13337245135054	39.0	38.5	39.0	35.0	39.0
14-15	38.65650126455415	41.0	40.0	41.0	36.0	41.0
16-17	38.637501043979924	41.0	40.0	41.0	35.0	41.0
18-19	38.59162053524583	41.0	39.5	41.0	35.0	41.0
20-21	38.62022365797718	41.0	40.0	41.0	35.0	41.0
22-23	38.57262202786944	41.0	39.0	41.0	35.0	41.0
24-25	38.582217006486594	41.0	39.0	41.0	35.0	41.0
26-27	38.57803145002923	41.0	39.0	41.0	35.0	41.0
28-29	38.54523870199566	41.0	39.0	41.0	35.0	41.0
30-31	38.46465758529048	41.0	39.0	41.0	34.0	41.0
32-33	38.442069714302846	41.0	39.0	41.0	34.0	41.0
34-35	38.3833359388217	41.0	39.0	41.0	34.0	41.0
36-37	38.256496981559565	41.0	39.0	41.0	34.0	41.0
38-39	38.15385415975142	41.0	39.0	41.0	33.5	41.0
40-41	38.050775971543786	41.0	38.0	41.0	33.0	41.0
42-43	37.92127449069841	40.5	38.0	41.0	33.0	41.0
44-45	37.74395701586636	40.0	38.0	41.0	33.0	41.0
46-47	37.60137719690853	40.0	38.0	41.0	33.0	41.0
48-49	37.46146953827177	40.0	37.0	41.0	33.0	41.0
50-51	37.272408092289965	40.0	37.0	41.0	32.0	41.0
52-53	37.090922621278345	40.0	36.5	41.0	32.0	41.0
54-55	36.899992290609745	40.0	36.0	41.0	32.0	41.0
56-57	36.62951090343346	39.5	35.0	41.0	31.0	41.0
58-59	36.39275809860739	39.0	35.0	41.0	31.0	41.0
60-61	36.15911431954994	39.0	35.0	41.0	31.0	41.0
62-63	35.82623698237333	38.0	35.0	40.0	30.5	41.0
64-65	35.59639050631421	37.5	35.0	40.0	30.0	41.0
66-67	35.366460726010416	37.0	35.0	40.0	30.0	41.0
68-69	35.11954351843722	36.5	35.0	39.5	30.0	41.0
70-71	34.89225998633725	36.0	35.0	39.0	30.0	41.0
72-73	34.56902131432255	36.0	35.0	39.0	30.0	41.0
74-75	34.21302994027364	35.0	35.0	38.0	30.0	40.5
76-77	33.89109201371414	35.0	34.0	37.0	29.0	39.5
78-79	33.593241862845666	35.0	34.0	37.0	29.0	39.0
80-81	33.292306028529026	35.0	34.0	36.0	29.0	39.0
82-83	33.02246216509659	35.0	34.0	36.0	29.0	37.0
84-85	32.77882594552459	35.0	34.0	35.5	29.0	37.0
86-87	32.550836147617694	35.0	34.0	35.0	28.5	37.0
88-89	32.26186443037243	35.0	34.0	35.0	27.5	36.0
90-91	32.05306715949658	35.0	34.0	35.0	27.0	36.0
92-93	31.89388538278193	35.0	34.0	35.0	27.0	36.0
94-95	31.755219578424843	35.0	34.0	35.0	26.0	36.0
96-97	31.602626332279	35.0	33.0	35.0	25.0	35.0
98-99	31.442639566732268	35.0	33.0	35.0	25.0	35.0
100	31.298592393829917	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.1298857939513134
1101	2	-0.1515780843423009
1101	3	-0.02938433432653298
1101	4	0.1072310798162448
1101	5	0.08565570406881307
1101	6	0.06093929915896723
1101	7	0.1202858435915175
1101	8	0.04242487147951124
1101	9	0.18276136438353063
1101	10-11	0.1551657276049241
1101	12-13	0.21709441341348423
1101	14-15	0.1678503013115673
1101	16-17	0.14394681909062967
1101	18-19	0.12007411350197827
1101	20-21	0.07612944664272447
1101	22-23	0.07363893087636342
1101	24-25	0.16003854357768432
1101	26-27	0.1407943656465349
1101	28-29	0.04675876184160899
1101	30-31	0.03308628888306231
1101	32-33	0.05397427782593667
1101	34-35	0.06876197304056575
1101	36-37	0.05667634159733126
1101	38-39	0.20547296730104136
1101	40-41	0.22580734734580687
1101	42-43	0.23640864591023103
1101	44-45	0.0847259657705024
1101	46-47	-0.04948720390665784
1101	48-49	-0.08593524165117117
1101	50-51	0.04927971897324568
1101	52-53	-0.1360189631218347
1101	54-55	-0.04404673850606855
1101	56-57	-0.04839402599995424
1101	58-59	0.02430733671295826
1101	60-61	0.07334816257027654
1101	62-63	0.0029630333497152606
1101	64-65	-0.06531236571955645
1101	66-67	0.004823511482712206
1101	68-69	0.060547810559995696
1101	70-71	0.008405962922743981
1101	72-73	0.04935925413529674
1101	74-75	0.10806856991991509
1101	76-77	-0.036927734768255505
1101	78-79	-0.11126362817968527
1101	80-81	-0.1560034959363179
1101	82-83	-0.045874938171479585
1101	84-85	-0.05222915296608477
1101	86-87	-0.1626335860309709
1101	88-89	-0.07713355279454248
1101	90-91	-0.09015405144117494
1101	92-93	-0.025754842142902135
1101	94-95	-0.16473236138499203
1101	96-97	-0.11272651601828443
1101	98-99	-0.12632305075634775
1101	100	-0.09381196329589514
1102	1	-0.42382576024289875
1102	2	-0.4882689646377578
1102	3	-0.32876596100957656
1102	4	-0.2473664650408196
1102	5	-0.037447371100611804
1102	6	-0.10488906566889256
1102	7	-0.1359563709470777
1102	8	-0.09040160954565835
1102	9	-0.17617888708222296
1102	10-11	0.01803167012208462
1102	12-13	-0.03096650557473879
1102	14-15	-0.151370044914664
1102	16-17	-0.16391873906133725
1102	18-19	-0.23120673180385154
1102	20-21	-0.15043150995542476
1102	22-23	-0.20072145239770833
1102	24-25	-0.1736973743979604
1102	26-27	-0.08840062900955559
1102	28-29	-0.07387869334679209
1102	30-31	-0.08870240201871837
1102	32-33	-0.11353062179688322
1102	34-35	-0.15267969396363412
1102	36-37	-0.13173327100389542
1102	38-39	-0.20307746347726408
1102	40-41	-0.15150421659065216
1102	42-43	-0.20565429574809713
1102	44-45	-0.31405879964687955
1102	46-47	-0.2313744906769699
1102	48-49	-0.3093015243108397
1102	50-51	-0.39085106437953243
1102	52-53	-0.3909161061726394
1102	54-55	-0.3379120015647743
1102	56-57	-0.37348482710792297
1102	58-59	-0.40698294413813585
1102	60-61	-0.3525170930491086
1102	62-63	-0.4403981740363747
1102	64-65	-0.31373409202344504
1102	66-67	-0.30790829585196633
1102	68-69	-0.32090849125180654
1102	70-71	-0.2547138052678548
1102	72-73	-0.19053462801089438
1102	74-75	-0.2066252563486657
1102	76-77	-0.16497331221287936
1102	78-79	-0.1947863887978727
1102	80-81	-0.23135364021790394
1102	82-83	-0.26625123168430775
1102	84-85	-0.2284809435705384
1102	86-87	-0.2389454698584501
1102	88-89	-0.3089215658910547
1102	90-91	-0.42920309478516927
1102	92-93	-0.40751016353418734
1102	94-95	-0.45750022052052586
1102	96-97	-0.5935080502883707
1102	98-99	-0.6365520208147117
1102	100	-0.5388562148872893
1103	1	-0.3503757617393326
1103	2	-0.4095729565553974
1103	3	-0.35841313440897693
1103	4	-0.23917612196935067
1103	5	-0.18151708709376635
1103	6	-0.09467004446564431
1103	7	-0.08263319718206219
1103	8	-0.10415160651235311
1103	9	-0.1236385096736754
1103	10-11	-0.06963141076701618
1103	12-13	-0.11577048899911091
1103	14-15	-0.1271075033886433
1103	16-17	-0.1664191981023535
1103	18-19	-0.18261006262155632
1103	20-21	-0.12798359594290076
1103	22-23	-0.10801427930568508
1103	24-25	-0.10680569675071183
1103	26-27	-0.12236470894981721
1103	28-29	-0.11302791443306859
1103	30-31	-0.09859493789064544
1103	32-33	-0.09981520457107962
1103	34-35	-0.07307268159661362
1103	36-37	-0.06319561791271155
1103	38-39	-0.034001943749828456
1103	40-41	-0.03959254740431106
1103	42-43	-0.030756841868573304
1103	44-45	-0.160759427259066
1103	46-47	-0.3490377212093847
1103	48-49	-0.2898934930750272
1103	50-51	-0.20766519038567566
1103	52-53	-0.365686444115795
1103	54-55	-0.2578628336220916
1103	56-57	-0.1521381868431888
1103	58-59	-0.21294014372107029
1103	60-61	-0.2548099118682572
1103	62-63	-0.19772756244816492
1103	64-65	-0.27295517426013305
1103	66-67	-0.23449965148297025
1103	68-69	-0.26128624357674113
1103	70-71	-0.27637417073729864
1103	72-73	-0.21162483219718808
1103	74-75	-0.34072933368434377
1103	76-77	-0.2956650162297052
1103	78-79	-0.2843408399587446
1103	80-81	-0.25706812641958265
1103	82-83	-0.34168754308154803
1103	84-85	-0.4674666894373516
1103	86-87	-0.3303264795545289
1103	88-89	-0.28677748849067797
1103	90-91	-0.24246733739177984
1103	92-93	-0.25342880367273324
1103	94-95	-0.2490737645516603
1103	96-97	-0.2971574464977813
1103	98-99	-0.24653086448435602
1103	100	-0.20312312842499836
1104	1	-0.4298997834760385
1104	2	-0.3553654753157822
1104	3	-0.27879474035016116
1104	4	-0.14776759564364284
1104	5	-0.12934880532053938
1104	6	-0.09392186224939536
1104	7	-0.1723999169574313
1104	8	-0.17265484400729036
1104	9	-0.18952330359345382
1104	10-11	-0.15315635292513008
1104	12-13	-0.12945835934008443
1104	14-15	-0.18140989095013538
1104	16-17	-0.22370994543987166
1104	18-19	-0.1631513053652256
1104	20-21	-0.19191331963200753
1104	22-23	-0.16566974848273475
1104	24-25	-0.13091787359589802
1104	26-27	-0.20810710940498467
1104	28-29	-0.11059030259652758
1104	30-31	-0.13668582076702762
1104	32-33	-0.022986959284594377
1104	34-35	-0.03854775206031036
1104	36-37	-0.06901626389708326
1104	38-39	-0.13281660990773503
1104	40-41	-0.11258075183496175
1104	42-43	-0.1281235433908563
1104	44-45	-0.16497365631389727
1104	46-47	-0.2809586737096197
1104	48-49	-0.2548966533827226
1104	50-51	-0.2710501857690062
1104	52-53	-0.25256966225081356
1104	54-55	-0.2114611667660995
1104	56-57	-0.27865991626453024
1104	58-59	-0.22753154166449008
1104	60-61	-0.29298351616277074
1104	62-63	-0.19715652251760218
1104	64-65	-0.2821509414867265
1104	66-67	-0.23439532734705182
1104	68-69	-0.25840070302819385
1104	70-71	-0.24042258801053862
1104	72-73	-0.22060369842830596
1104	74-75	-0.1490639049262228
1104	76-77	-0.1477170316904619
1104	78-79	-0.18063842540598785
1104	80-81	-0.24027024144700704
1104	82-83	-0.27229286579702006
1104	84-85	-0.2507154566260752
1104	86-87	-0.290106879432976
1104	88-89	-0.19480539823112508
1104	90-91	-0.1936140776144839
1104	92-93	-0.11584395839188488
1104	94-95	-0.05598925569066182
1104	96-97	-0.0782587579041163
1104	98-99	-0.049879674101788396
1104	100	-0.006118478743406541
1105	1	-0.2360929911023426
1105	2	-0.2754169384566758
1105	3	-0.20968324971462593
1105	4	-0.20349096780631726
1105	5	-0.09415983881008572
1105	6	-0.07667150279150547
1105	7	-0.02972647823879271
1105	8	-0.0961108917846829
1105	9	-0.057932163679318194
1105	10-11	0.0013990768955594035
1105	12-13	-0.0359447936617201
1105	14-15	-0.1207970564362455
1105	16-17	-0.1514145789320267
1105	18-19	-0.15558350481134653
1105	20-21	-0.1097256350604141
1105	22-23	-0.12758043589983004
1105	24-25	-0.22163989329683176
1105	26-27	-0.15465564097487317
1105	28-29	-0.18193309991423945
1105	30-31	-0.14513158473159393
1105	32-33	-0.16443079454129617
1105	34-35	-0.15075414839238022
1105	36-37	-0.11279477752539435
1105	38-39	-0.1711237082671957
1105	40-41	-0.2102392995555178
1105	42-43	-0.2121203253206687
1105	44-45	-0.2895397898369012
1105	46-47	-0.27580665283484507
1105	48-49	-0.3064865034705804
1105	50-51	-0.2865744514379571
1105	52-53	-0.3392948443459858
1105	54-55	-0.2530077241594242
1105	56-57	-0.23444736217186346
1105	58-59	-0.3049191237923239
1105	60-61	-0.33298033335402266
1105	62-63	-0.26225549348676225
1105	64-65	-0.30929463973418336
1105	66-67	-0.27766811771919464
1105	68-69	-0.23206992011756
1105	70-71	-0.21967164929073846
1105	72-73	-0.20664819557931935
1105	74-75	-0.174330766737107
1105	76-77	-0.1320904614545455
1105	78-79	-0.1495875742366337
1105	80-81	-0.24506027582227574
1105	82-83	-0.22139117051059287
1105	84-85	-0.25331691561447656
1105	86-87	-0.2207300464761488
1105	88-89	-0.15381411569374848
1105	90-91	-0.09820933894118156
1105	92-93	-0.15939410781222563
1105	94-95	-0.23291662965537796
1105	96-97	-0.23384307936965243
1105	98-99	-0.3171630447612088
1105	100	-0.2771286122311487
1106	1	-0.14140177561688105
1106	2	-0.2082433666552106
1106	3	-0.2187702146802657
1106	4	-0.0714585722029426
1106	5	-0.035984412216897965
1106	6	0.002134428265826216
1106	7	-0.036622526160485336
1106	8	-0.1723421189227281
1106	9	-0.0875772053631323
1106	10-11	-0.11574741245230769
1106	12-13	-0.06729741077105444
1106	14-15	-0.14396106207367154
1106	16-17	-0.09317411881539073
1106	18-19	-0.16699586367523267
1106	20-21	-0.08757232164693818
1106	22-23	-0.1069973257294734
1106	24-25	-0.15026876390506771
1106	26-27	-0.21956123546145534
1106	28-29	-0.2271562985301827
1106	30-31	-0.22736447066993293
1106	32-33	-0.1657003736407745
1106	34-35	-0.2379348382466162
1106	36-37	-0.22424415204543635
1106	38-39	-0.2287229200252554
1106	40-41	-0.256014436794068
1106	42-43	-0.2754927322202789
1106	44-45	-0.3297134471619003
1106	46-47	-0.27482611656663636
1106	48-49	-0.23154347461885294
1106	50-51	-0.18452562818838203
1106	52-53	-0.2288326430770269
1106	54-55	-0.29885324014252745
1106	56-57	-0.2339657577502976
1106	58-59	-0.2596790877299
1106	60-61	-0.2307033582502882
1106	62-63	-0.12264954318662546
1106	64-65	-0.2553270157384162
1106	66-67	-0.1441828019324376
1106	68-69	-0.16159086358471342
1106	70-71	-0.2723173555637146
1106	72-73	-0.24047974806315153
1106	74-75	-0.13336793205944986
1106	76-77	-0.10456871976769833
1106	78-79	-0.15544696106849898
1106	80-81	-0.1485699925433721
1106	82-83	-0.0819919020931934
1106	84-85	-0.11799716448796715
1106	86-87	-0.13993729673406108
1106	88-89	-0.08378730940990664
1106	90-91	-0.1149069633467441
1106	92-93	-0.13884908234224724
1106	94-95	-0.1607238952362522
1106	96-97	-0.1505839555226629
1106	98-99	-0.1925162747892344
1106	100	-0.07374277100915805
1107	1	-0.10460587527685661
1107	2	-0.15057966807598788
1107	3	-0.0128137281905083
1107	4	-0.05436769548487774
1107	5	-0.09756868290924103
1107	6	-0.06537141024048054
1107	7	-0.10927549848311457
1107	8	-0.0562978517222561
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2216	30-31	-0.06873701105036645
2216	32-33	-0.13804077538685533
2216	34-35	-0.12907182332286027
2216	36-37	-0.13128772911910858
2216	38-39	-0.07915911163128442
2216	40-41	-0.07570513729161377
2216	42-43	-0.06554956273225088
2216	44-45	0.0693477183041935
2216	46-47	0.04757271367967064
2216	48-49	0.06568720517718418
2216	50-51	0.019475392419749937
2216	52-53	-0.029961625059875985
2216	54-55	-0.004271998551693912
2216	56-57	-0.026672092228309907
2216	58-59	-0.0642327294990821
2216	60-61	-0.08081094297325109
2216	62-63	-0.11312579629218789
2216	64-65	-0.19610507818753575
2216	66-67	-0.18559502897566205
2216	68-69	-0.21259391357215662
2216	70-71	-0.21967824180930506
2216	72-73	-0.2493961607834052
2216	74-75	-0.18708026261022326
2216	76-77	-0.18688543446170058
2216	78-79	-0.20587115132248357
2216	80-81	-0.24743341518430384
2216	82-83	-0.20683726549412995
2216	84-85	-0.16837512487627748
2216	86-87	-0.21334368158485972
2216	88-89	-0.20048914716215194
2216	90-91	-0.19964969381544861
2216	92-93	-0.17500060713868493
2216	94-95	-0.17529373389862357
2216	96-97	-0.20994941609180984
2216	98-99	-0.23363011205221795
2216	100	-0.2042529040959309
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	29437.0
3	4669.0
4	3046.0
5	2558.0
6	2335.0
7	2497.0
8	2541.0
9	2773.0
10	3135.0
11	3408.0
12	3518.0
13	3792.0
14	4162.0
15	4127.0
16	4378.0
17	4475.0
18	4418.0
19	4626.0
20	4732.0
21	4990.0
22	5884.0
23	7177.0
24	8865.0
25	11622.0
26	14226.0
27	15416.0
28	16986.0
29	21094.0
30	25112.0
31	28022.0
32	35873.0
33	47996.0
34	71475.0
35	119782.0
36	250489.0
37	546058.0
38	740322.0
39	268181.0
40	618.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.09503171160708	18.214253203625145	13.548390993480798	24.142324091286977
2	16.055204071158517	18.793375133515635	39.38001502332739	25.771405771998456
3	19.458987970972977	23.328255565609926	28.245344222403652	28.967412241013445
4	13.608403292764379	15.466972490856415	35.664469629123744	35.260154587255464
5	15.175042967317353	36.50036031563478	33.618951829364136	14.705644887683732
6	34.30001797305928	34.779885787428036	16.658709620133603	14.261386619379076
7	30.329975254000317	30.464984624885812	20.328988845000836	18.876051276113035
8	26.917719928540862	35.119097137946504	18.960514793980348	19.002668139532293
9	26.34789116258923	16.35777650547768	19.418373014086075	37.87595931784701
10-11	25.98935482722821	25.665033840791544	26.94064064008261	21.404970691897635
12-13	26.079159519725557	22.69695540308748	27.24819897084048	23.975686106346483
14-15	23.35871463350053	24.58917348009678	24.18747500908517	27.864636877317523
16-17	23.846522931901074	27.82379127386301	24.741077607683156	23.588608186552758
18-19	24.029736987388045	27.15182050608622	25.969952135670987	22.84849037085475
20-21	24.693891597582997	26.249193520547347	25.805348398051454	23.251566483818202
22-23	24.56080124142761	25.968493006462946	25.777484350820373	23.693221401289073
24-25	24.28934352436042	26.070350504695117	25.71506246753127	23.925243503413196
26-27	24.035734026527365	26.24681229516035	25.780540109871026	23.936913568441263
28-29	24.07781887248317	26.299656092421703	25.766909182368792	23.855615852726334
30-31	24.144235774567928	26.385881380337082	25.599989022891563	23.86989382220343
32-33	24.062239254833727	26.25815840271531	25.79536655035592	23.884235792095044
34-35	24.16572522471344	26.19885397752973	25.809043416373157	23.826377381383672
36-37	24.34721199696937	26.16741181782437	25.682459697734615	23.80291648747164
38-39	24.147884097702445	26.317563171365588	25.64876744315172	23.885785287780248
40-41	24.165610169469726	26.186088708035054	25.81342423853956	23.834876883955662
42-43	24.20633653033341	26.198028203376282	25.701895410782388	23.893739855507917
44-45	24.015598896303697	26.32259085225189	25.742595058320074	23.919215193124344
46-47	24.19782370258698	26.172274632988856	25.806549816526587	23.82335184789758
48-49	24.17036435691702	26.20470673327261	25.653627802314716	23.971301107495655
50-51	24.05041175905664	26.321560499941693	25.715069078112457	23.91295866288921
52-53	24.127504146064968	26.240175069415507	25.682192758149352	23.950128026370173
54-55	24.141061656110814	26.25113833394216	25.733164233933415	23.874635776013612
56-57	24.039371820773063	26.212157981210186	25.793499027581024	23.95497117043572
58-59	24.09676187013437	26.07126947056193	25.76416957830951	24.06779908099419
60-61	24.223793296242686	26.15769642379381	25.661513250416757	23.95699702954675
62-63	24.061189247825887	26.28387120287188	25.717235014347594	23.937704534954644
64-65	24.117810485048278	26.166338036200372	25.70533358886335	24.010517889888
66-67	24.056215953851705	26.08610549474203	25.71244871571963	24.145229835686635
68-69	24.05483548090913	26.24313543565451	25.723425451104536	23.978603632331822
70-71	24.08491027582076	26.034611909855187	25.78231826303285	24.098159551291204
72-73	24.08039591613926	26.16233470797068	25.789437347797545	23.967832028092516
74-75	24.052626263427655	26.213466283472243	25.734180203589368	23.999727249510734
76-77	24.100522844512557	26.156547198042663	25.63989869418581	24.103031263258977
78-79	24.163007433811675	26.1412336315391	25.763143276198484	23.932615658450732
80-81	23.984549958030176	26.154845612143056	25.87240958262803	23.98819484719874
82-83	24.07376758177961	26.13009187717923	25.832845962943846	23.963294578097315
84-85	24.124521127014226	26.092799516989	25.691133677924633	24.09154567807214
86-87	24.069005880549593	26.29478211095223	25.7050763956926	23.931135612805576
88-89	24.002200243752494	26.22524456140729	25.74546199726048	24.02709319757973
90-91	24.043778948556692	26.140885296210687	25.749540848559775	24.065794906672842
92-93	24.06596460047451	26.26571298969268	25.72159981622525	23.946722593607568
94-95	24.0494012073029	26.047341959887	25.852344013519858	24.050912819290243
96-97	24.09831236331767	26.157180738368584	25.73171885305842	24.012788045255324
98-99	24.03973394077921	26.270896700436296	25.722182494232843	23.96718686455165
100	24.276222178257854	25.978463986702412	25.57777388049966	24.167539954540075
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2301.0
1	1414.0
2	501.0
3	425.5
4	329.0
5	239.0
6	196.5
7	209.5
8	216.0
9	191.0
10	160.0
11	157.0
12	157.5
13	178.0
14	190.5
15	201.0
16	225.5
17	270.0
18	372.0
19	477.0
20	660.5
21	949.0
22	1398.0
23	2188.5
24	3290.0
25	4785.5
26	6899.0
27	9426.5
28	12630.0
29	16635.5
30	20532.5
31	24524.0
32	28720.0
33	32670.5
34	36538.5
35	40708.5
36	45349.0
37	50477.0
38	55893.5
39	60383.5
40	64424.5
41	68937.5
42	71847.0
43	73512.5
44	76324.0
45	78594.0
46	80005.5
47	79961.5
48	79594.0
49	80790.5
50	81676.0
51	82239.5
52	81494.5
53	81425.5
54	82022.0
55	79439.0
56	75394.5
57	70439.0
58	65059.5
59	61453.0
60	57372.5
61	51396.5
62	45740.5
63	40084.5
64	33630.0
65	28358.0
66	23324.5
67	18611.5
68	14677.0
69	11118.0
70	8478.5
71	6420.0
72	4996.5
73	3827.0
74	2897.0
75	2118.5
76	1466.0
77	1096.5
78	828.5
79	561.5
80	370.0
81	210.5
82	130.0
83	105.5
84	77.5
85	53.5
86	46.0
87	41.5
88	42.0
89	35.5
90	22.5
91	22.0
92	21.0
93	11.0
94	7.0
95	7.0
96	6.0
97	7.5
98	6.0
99	5.5
100	17.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.7235691050468667
2	0.2756535314361095
3	0.27256977533551907
4	0.2861040382214437
5	0.2698714887475025
6	0.1519606478457608
7	0.13384358075479214
8	0.12219383548589503
9	0.13191623319192314
10-11	0.12275062478183496
12-13	0.12056629754391675
14-15	0.11639037782436724
16-17	0.11345652653422221
18-19	0.11574792863674424
20-21	0.12508485683019854
22-23	0.12778314341821514
24-25	0.11836055533307778
26-27	0.12026648792302602
28-29	0.12585579585534615
30-31	0.11516972436788353
32-33	0.10951617151680113
34-35	0.10279187001968036
36-37	0.11247143777986693
38-39	0.10658232022665608
40-41	0.1037341288281941
42-43	0.11035135546071102
44-45	0.10581138120150849
46-47	0.11122936935046245
48-49	0.10238498553418579
50-51	0.1020637609403743
52-53	0.10634675552452764
54-55	0.10531883682433082
56-57	0.10651807530789377
58-59	0.10758882395393213
60-61	0.10754599400809056
62-63	0.10889513730209888
64-65	0.10313450958641263
66-67	0.11112229448585861
68-69	0.105211761959727
70-71	0.11187181853808546
72-73	0.12018082803134296
74-75	0.12906804179346115
76-77	0.11429171047813211
78-79	0.11566226874506118
80-81	0.11917432430406691
82-83	0.11011579075858259
84-85	0.11893875960193848
86-87	0.10921636189591037
88-89	0.7233977852635005
90-91	0.7843662131689234
92-93	0.8110492694281988
94-95	0.8311579290007988
96-97	0.8430646539447451
98-99	0.8465981244766716
100	0.8484183971749368
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2334815.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.16752905665469
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.20555903682025	52.91965628093871
2	10.935657343753693	14.252995354053757
3	3.2235437179162254	6.302111367081069
4	1.4463802764913414	3.770281147808869
5	0.8422024423076577	2.744212606533491
6	0.5170425291486388	2.021663042509209
7	0.3710091622152584	1.6924425251263575
8	0.25066260229220727	1.3068049934635275
9	0.19576518234692186	1.1481779887986898
>10	0.9617380808900862	10.918044777416737
>50	0.03993928384611733	1.7668515168894197
>100	0.010302715011989483	1.0316617331237752
>500	1.3175130638322762E-4	0.04868209318313037
>1k	6.587565319085159E-5	0.0764145730732485
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	2.1414972920766743E-5	0.0	0.0	0.0
16-17	0.0	4.2829945841533485E-5	0.0	0.0	0.0
18-19	0.0	4.2829945841533485E-5	0.0	2.1414972920766743E-5	0.0
20-21	0.0	4.2829945841533485E-5	0.0	4.2829945841533485E-5	0.0
22-23	0.0	4.2829945841533485E-5	0.0	1.499048104453672E-4	0.0
24-25	0.0	4.2829945841533485E-5	0.0	2.569796750492009E-4	0.0
26-27	0.0	4.2829945841533485E-5	0.0	5.782042688607021E-4	0.0
28-29	0.0	4.2829945841533485E-5	0.0	0.0014776331315329051	0.0
30-31	0.0	4.2829945841533485E-5	0.0	0.0046256341508856165	0.0
32-33	0.0	4.2829945841533485E-5	0.0	0.01137135062092714	0.0
34-35	0.0	4.2829945841533485E-5	0.0	0.02199317718962744	0.0
36-37	0.0	6.424491876230022E-5	0.0	0.03670526358619419	0.0
38-39	0.0	8.565989168306697E-5	0.0	0.056299963808695765	0.0
40-41	0.0	8.565989168306697E-5	0.0	0.07381741165788297	0.0
42-43	0.0	8.565989168306697E-5	0.0	0.09109929480494172	0.0
44-45	0.0	8.565989168306697E-5	0.0	0.11182898859224392	0.0
46-47	0.0	1.070748646038337E-4	0.0	0.13737705128671865	0.0
48-49	2.1414972920766743E-5	1.7131978336613394E-4	0.0	0.16159738566010584	0.0
50-51	4.2829945841533485E-5	1.7131978336613394E-4	0.0	0.18493970614374158	0.0
52-53	4.2829945841533485E-5	1.7131978336613394E-4	0.0	0.2066330737124783	0.0
54-55	4.2829945841533485E-5	1.7131978336613394E-4	0.0	0.2309604829504693	0.0
56-57	4.2829945841533485E-5	1.927347562869007E-4	0.0	0.25939956698924754	0.0
58-59	4.2829945841533485E-5	2.141497292076674E-4	0.0	0.28826695048644113	0.0
60-61	4.2829945841533485E-5	2.141497292076674E-4	0.0	0.3176268783608123	0.0
62-63	4.2829945841533485E-5	2.141497292076674E-4	0.0	0.34578756775162056	0.0
64-65	4.2829945841533485E-5	2.141497292076674E-4	0.0	0.37227788925460903	0.0
66-67	4.2829945841533485E-5	2.141497292076674E-4	0.0	0.40452883847328375	0.0
68-69	4.2829945841533485E-5	2.569796750492009E-4	0.0	0.43969222400918273	0.0
70-71	4.2829945841533485E-5	2.569796750492009E-4	0.0	0.47498409938260633	0.0
72-73	4.2829945841533485E-5	3.212245938115011E-4	0.0	0.5090124913537046	0.0
74-75	4.2829945841533485E-5	3.854695125738014E-4	0.0	0.5441972918625244	0.0
76-77	4.2829945841533485E-5	4.282994584153348E-4	0.0	0.5816306645280247	0.0
78-79	4.2829945841533485E-5	4.282994584153348E-4	0.0	0.6227259975629761	0.0
80-81	4.2829945841533485E-5	4.92544377177635E-4	0.0	0.6634572760582744	0.0
82-83	4.2829945841533485E-5	5.567892959399352E-4	0.0	0.7054734529288188	0.0
84-85	4.2829945841533485E-5	5.782042688607021E-4	0.0	0.7491814126601037	0.0
86-87	4.2829945841533485E-5	5.996192417814688E-4	0.0	0.792589562770498	0.0
88	4.2829945841533485E-5	5.996192417814688E-4	0.0	0.8292305814379298	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3125	0.0	37.483074	1
GGTATCA	2065	0.0	35.681087	1
TCAACGC	3495	0.0	32.56358	4
ATCAACG	3625	0.0	31.525513	3
CAACGCA	3905	0.0	29.142107	5
AACGCAG	4000	0.0	28.761896	6
TAATACG	115	4.178848E-6	28.626228	4
TATCAAC	4175	0.0	27.37304	2
GTACATG	8870	0.0	25.399683	1
TACATGG	8975	0.0	24.314018	2
ACGCAGA	4840	0.0	23.66959	7
ACATGGG	9570	0.0	22.064714	3
CGCAGAG	5340	0.0	21.27703	8
TAGACCG	135	4.0643092E-4	20.899895	5
TTAAACG	120	0.005047749	19.595335	4
TATACTG	1105	0.0	18.724792	5
CATGGGG	8055	0.0	18.62469	4
GAGTACA	5995	0.0	17.805445	1
GCAGAGT	6470	0.0	17.633516	9
AGAGTAC	5145	0.0	17.611065	10-11
>>END_MODULE