FastQCFastQC Report
Wed 25 May 2016
SRR2049390_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049390_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2334815
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40560.17371826033325982No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30870.13221604281281385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29100.047.761961
GTATCAA50150.041.618451
TCAACGC62400.032.627284
ATCAACG62650.032.4220353
CAACGCA66050.030.824265
TATCAAC67300.030.389522
AACGCAG68600.029.8072416
ACGCAGA81400.025.00147
GTACATG79200.024.9253921
TACATGG81500.023.9408822
CGCAGAG85300.023.8583138
ACATGGG84700.021.9276083
GCAGAGT97250.020.7333059
GTGGTAT16550.020.2121941
AGAGTAC86000.020.13779810-11
CATGGGG72500.019.5860354
CAGAGTA89600.018.80416710-11
GAGTACT50900.018.69735512-13
AGTACTT55150.017.29909712-13
GTACTTT55650.017.01699314-15
Produced by FastQC (version 0.11.5)