##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049390_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2334815 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.01985339309538 34.0 33.0 34.0 31.0 34.0 2 33.12651966001589 34.0 33.0 34.0 31.0 34.0 3 33.22357317389172 34.0 33.0 34.0 31.0 34.0 4 36.55457969903397 37.0 37.0 37.0 35.0 37.0 5 36.521462300010924 37.0 37.0 37.0 35.0 37.0 6 36.553083220726265 37.0 37.0 37.0 35.0 37.0 7 36.53483423740211 37.0 37.0 37.0 35.0 37.0 8 36.53787430695794 37.0 37.0 37.0 35.0 37.0 9 38.35342671689192 39.0 39.0 39.0 37.0 39.0 10-11 38.36910590346558 39.0 39.0 39.0 37.0 39.0 12-13 38.3659906673548 39.0 39.0 39.0 37.0 39.0 14-15 40.017541860918314 41.0 40.0 41.0 38.0 41.0 16-17 40.029334229906866 41.0 40.0 41.0 38.0 41.0 18-19 40.02905797675619 41.0 40.0 41.0 38.0 41.0 20-21 40.01850489225056 41.0 40.0 41.0 38.0 41.0 22-23 39.99334444056595 41.0 40.0 41.0 38.0 41.0 24-25 39.97104267361654 41.0 40.0 41.0 38.0 41.0 26-27 39.90814090195583 41.0 40.0 41.0 38.0 41.0 28-29 39.84412812149999 41.0 40.0 41.0 38.0 41.0 30-31 39.76579043735799 41.0 40.0 41.0 37.0 41.0 32-33 39.676478436193015 41.0 40.0 41.0 37.0 41.0 34-35 39.60700226784563 41.0 40.0 41.0 36.5 41.0 36-37 39.52442998695828 41.0 40.0 41.0 36.0 41.0 38-39 39.43770598527078 41.0 40.0 41.0 36.0 41.0 40-41 39.336343136394106 41.0 40.0 41.0 35.0 41.0 42-43 39.221622484008364 41.0 39.0 41.0 35.0 41.0 44-45 39.086926587331334 41.0 39.0 41.0 35.0 41.0 46-47 38.89579602666592 41.0 39.0 41.0 35.0 41.0 48-49 38.69365367277493 41.0 39.0 41.0 35.0 41.0 50-51 38.519768803952346 41.0 38.0 41.0 35.0 41.0 52-53 38.37011390624096 40.0 38.0 41.0 35.0 41.0 54-55 38.25590849810371 40.0 37.0 41.0 35.0 41.0 56-57 38.10057734766994 40.0 37.0 41.0 35.0 41.0 58-59 37.877576810154125 40.0 36.5 41.0 35.0 41.0 60-61 37.626282167966195 39.5 36.0 41.0 34.0 41.0 62-63 37.35161843657849 39.0 35.0 41.0 34.0 41.0 64-65 37.06463381467054 39.0 35.0 41.0 34.0 41.0 66-67 36.75086741347816 37.5 35.0 41.0 34.0 41.0 68-69 36.42072134194787 37.0 35.0 40.0 34.0 41.0 70-71 36.08708698547851 36.5 35.0 39.5 33.0 41.0 72-73 35.748337662727025 36.0 35.0 39.0 33.0 41.0 74-75 35.406773555934834 35.5 35.0 39.0 33.0 41.0 76-77 35.09586348383063 35.0 35.0 37.0 33.0 39.5 78-79 34.79310052402439 35.0 35.0 37.0 33.0 39.0 80-81 34.51749474797789 35.0 35.0 36.5 33.0 39.0 82-83 34.27501772089009 35.0 35.0 36.0 33.0 37.5 84-85 34.05515726085365 35.0 35.0 36.0 32.0 37.0 86-87 33.856087527277325 35.0 35.0 35.5 32.0 37.0 88-89 33.71032137449862 35.0 35.0 35.0 32.0 36.0 90-91 33.59111856828057 35.0 35.0 35.0 32.0 36.0 92-93 33.48140859125884 35.0 35.0 35.0 32.0 36.0 94-95 33.391609827759375 35.0 35.0 35.0 32.0 36.0 96-97 33.37381848240653 35.0 35.0 35.0 32.0 36.0 98-99 33.31937583919925 35.0 35.0 35.0 32.0 35.0 100 33.26861871283164 35.0 35.0 35.0 32.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.034087551238620506 1101 2 -0.022105057779810977 1101 3 0.009195167365220414 1101 4 -0.00791777260087656 1101 5 0.047313509042353985 1101 6 -0.03626479173169628 1101 7 -0.020227971172005255 1101 8 0.026047013007726605 1101 9 0.036829711755942185 1101 10-11 0.008736046237082462 1101 12-13 0.03394113195086845 1101 14-15 0.034660158429502985 1101 16-17 0.014902148119595893 1101 18-19 0.06116058805466196 1101 20-21 0.0292239704616577 1101 22-23 0.005889405873418241 1101 24-25 0.01026324419282787 1101 26-27 0.05168130738256593 1101 28-29 0.015072376406024546 1101 30-31 0.00432280619926928 1101 32-33 0.08855958868398517 1101 34-35 0.05212397967926563 1101 36-37 0.08173289173760168 1101 38-39 0.10547318142361206 1101 40-41 0.14011288613834694 1101 42-43 0.15411383114700783 1101 44-45 0.20602639890999797 1101 46-47 0.17070347455446466 1101 48-49 0.22932552342614798 1101 50-51 0.18368112992052232 1101 52-53 0.26266077773574636 1101 54-55 0.2522906690548936 1101 56-57 0.2898362141461419 1101 58-59 0.3044391468646026 1101 60-61 0.2974742708887561 1101 62-63 0.36345139118437686 1101 64-65 0.31121217458922246 1101 66-67 0.34304918349732816 1101 68-69 0.3338039964643187 1101 70-71 0.27992467884563155 1101 72-73 0.3207384957886248 1101 74-75 0.26161111393165015 1101 76-77 0.25318681739130966 1101 78-79 0.2081778378920589 1101 80-81 0.24249988807538614 1101 82-83 0.2664555959872885 1101 84-85 0.24654500594838424 1101 86-87 0.25910517695366053 1101 88-89 0.1767093645479605 1101 90-91 0.17392985203271394 1101 92-93 0.15052701969592164 1101 94-95 0.19016357289802244 1101 96-97 0.22792153875205656 1101 98-99 0.18177778367879682 1101 100 0.16280203312221175 1102 1 -0.15951934212412766 1102 2 -0.12962336472144642 1102 3 -0.15715188380990242 1102 4 -0.07160439677521424 1102 5 -0.13005469709641204 1102 6 -0.17206638325152568 1102 7 -0.027001477582636824 1102 8 -0.054697159313519705 1102 9 0.03942851633890143 1102 10-11 -0.005625056171851384 1102 12-13 -0.03937156689612209 1102 14-15 -0.05181828777632802 1102 16-17 -0.10092381959207586 1102 18-19 -0.012135372308613057 1102 20-21 0.008704242608743584 1102 22-23 -0.03710049648129399 1102 24-25 -0.03297422240253667 1102 26-27 -0.05982158527539383 1102 28-29 -0.062264584563138214 1102 30-31 -0.07386358115652314 1102 32-33 -0.03516498018640135 1102 34-35 -0.07960468412683497 1102 36-37 -0.18815242691766798 1102 38-39 -0.1008188146054394 1102 40-41 -0.1436325169780659 1102 42-43 -0.1204682853743364 1102 44-45 -0.11901170697144892 1102 46-47 -0.07416452700284282 1102 48-49 -0.045084971975107635 1102 50-51 -0.13912610804781167 1102 52-53 -0.13243563179750595 1102 54-55 -0.19697901643520055 1102 56-57 -0.17447310228725854 1102 58-59 -0.17636801175091676 1102 60-61 -0.13546661043994135 1102 62-63 -0.22207368471742228 1102 64-65 -0.1612290485351835 1102 66-67 -0.16490200956393153 1102 68-69 -0.16101988575627502 1102 70-71 -0.04581799321152147 1102 72-73 -0.03790077294215877 1102 74-75 -0.10024297691244044 1102 76-77 -0.16269837562201417 1102 78-79 -0.21832688979967685 1102 80-81 -0.12771052913028313 1102 82-83 -0.22998912940651906 1102 84-85 -0.15943162464857608 1102 86-87 -0.12507154175798263 1102 88-89 -0.17977043313543106 1102 90-91 -0.08782521311511005 1102 92-93 -0.13758454457117608 1102 94-95 -0.20946379952373206 1102 96-97 -0.2282649634999956 1102 98-99 -0.18582442646232522 1102 100 -0.0965673848001174 1103 1 -0.08794676066356288 1103 2 -0.05549633129112408 1103 3 -0.07835820312610764 1103 4 -0.06684755908645457 1103 5 -0.0861519749630304 1103 6 -0.0463696318058453 1103 7 -0.025496551081779728 1103 8 -0.04234369854878395 1103 9 -0.028871122980248742 1103 10-11 -0.061205518577814644 1103 12-13 -0.07768713311814679 1103 14-15 -0.12765064842805884 1103 16-17 -0.07750908837979864 1103 18-19 -0.09213287286066674 1103 20-21 -0.04339367589307841 1103 22-23 -0.0782959889008552 1103 24-25 -0.040823722472502766 1103 26-27 -0.15474984950183313 1103 28-29 -0.17764261999705866 1103 30-31 -0.2037350779861029 1103 32-33 -0.11281884385567054 1103 34-35 -0.1918811594684513 1103 36-37 -0.14748766274341563 1103 38-39 -0.22095399105919 1103 40-41 -0.23784067326717917 1103 42-43 -0.1943315218924795 1103 44-45 -0.21100807427867352 1103 46-47 -0.216334682785579 1103 48-49 -0.2451953035928014 1103 50-51 -0.20847325062877786 1103 52-53 -0.19972699867197008 1103 54-55 -0.19052140149092622 1103 56-57 -0.12139795221926164 1103 58-59 -0.11916725593080457 1103 60-61 -0.26695476071095925 1103 62-63 -0.2641710814898133 1103 64-65 -0.28887055651926374 1103 66-67 -0.3079515220906899 1103 68-69 -0.31604612187079084 1103 70-71 -0.26556880876763245 1103 72-73 -0.29411208306540715 1103 74-75 -0.33178962963395975 1103 76-77 -0.32545470047905667 1103 78-79 -0.31842057865318907 1103 80-81 -0.29499680004293793 1103 82-83 -0.26737175039718863 1103 84-85 -0.215508437779647 1103 86-87 -0.23347316502404425 1103 88-89 -0.2712361733076989 1103 90-91 -0.25419734532215443 1103 92-93 -0.21369425092244398 1103 94-95 -0.2662083454290425 1103 96-97 -0.2280156253683714 1103 98-99 -0.20311500467603594 1103 100 -0.2154357496353967 1104 1 -0.05931155726305093 1104 2 -0.040063738081471456 1104 3 -0.007904992670972888 1104 4 -0.04881778520137203 1104 5 0.0011062080979158395 1104 6 -0.025076148447709556 1104 7 -0.003271675042540778 1104 8 -0.010262351937306846 1104 9 -0.010393566019466505 1104 10-11 -0.03413415776194029 1104 12-13 -0.0072912062741750105 1104 14-15 -0.09245511227772596 1104 16-17 -0.08548325832570924 1104 18-19 -0.10351551476139775 1104 20-21 -0.018883475041199915 1104 22-23 -0.058023027639130476 1104 24-25 -0.13340962118925148 1104 26-27 -0.08738121587129655 1104 28-29 -0.07179954602256089 1104 30-31 -0.05408708092213743 1104 32-33 -0.03874642572026943 1104 34-35 -0.04891903364450911 1104 36-37 -0.023315143924776294 1104 38-39 -0.06147641759295652 1104 40-41 -0.053614201054095645 1104 42-43 -0.0797876984735808 1104 44-45 -0.04734200083719742 1104 46-47 -0.07847089544565478 1104 48-49 0.0026761733437510316 1104 50-51 -0.07809274181438042 1104 52-53 -0.08867165008177835 1104 54-55 -0.0037796932936444705 1104 56-57 -0.06336897447928891 1104 58-59 -0.09896978281769009 1104 60-61 -0.09365319550910556 1104 62-63 0.023776106904328742 1104 64-65 0.04313413685321876 1104 66-67 0.046351108197967505 1104 68-69 -0.1067075339797654 1104 70-71 -0.11817914525544637 1104 72-73 -0.10074916614508567 1104 74-75 -0.1032175838420244 1104 76-77 -0.09449756900929174 1104 78-79 -0.11673537248558574 1104 80-81 -0.10551295450674303 1104 82-83 -0.12441112948168609 1104 84-85 -0.11679003721575043 1104 86-87 -0.06700135972617716 1104 88-89 -0.05401346724933376 1104 90-91 -0.041953070527817715 1104 92-93 -0.1220317834357516 1104 94-95 -0.10760857048567374 1104 96-97 -0.08350596822534584 1104 98-99 -0.09226966362980704 1104 100 -0.0844435683628646 1105 1 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0.05980358989487655 1105 58-59 0.1335735087169141 1105 60-61 0.043050481748522884 1105 62-63 0.0719979328993432 1105 64-65 0.09833132484632756 1105 66-67 0.14275342079154285 1105 68-69 0.1362788036048883 1105 70-71 0.1436541061878387 1105 72-73 0.17916090790857453 1105 74-75 0.18240215217042532 1105 76-77 0.16584486595075987 1105 78-79 0.1100875858655499 1105 80-81 0.08098929178605374 1105 82-83 0.10503797086448685 1105 84-85 0.09960179901899835 1105 86-87 0.14376790102241443 1105 88-89 0.1821271405186735 1105 90-91 0.1629335152324103 1105 92-93 0.17250951397175385 1105 94-95 0.14548239613705505 1105 96-97 0.1014510383383822 1105 98-99 0.04435156681807939 1105 100 0.04952447085528178 1106 1 -0.056799271634723425 1106 2 -0.06149589132587607 1106 3 -0.06567023915851422 1106 4 -0.027817794318721667 1106 5 0.01754615667606174 1106 6 0.005651598381732015 1106 7 -0.018426533222168473 1106 8 -0.03028678643370597 1106 9 -0.04480289853831465 1106 10-11 -0.03600594959462455 1106 12-13 -0.061183402734812375 1106 14-15 -0.07193492190747008 1106 16-17 -0.13209069261240813 1106 18-19 -0.07667722918777997 1106 20-21 -0.053776482945949056 1106 22-23 -0.10884700809153713 1106 24-25 -0.10617040017557855 1106 26-27 -0.08995732294545178 1106 28-29 -0.1422962592180994 1106 30-31 -0.15041939919056802 1106 32-33 -0.11460778730522492 1106 34-35 -0.09704245520339327 1106 36-37 -0.09870399887686432 1106 38-39 -0.12156248537132797 1106 40-41 -0.06326833440444801 1106 42-43 -0.09872101418003609 1106 44-45 -0.052326683690196774 1106 46-47 -0.12868652075905374 1106 48-49 -0.06899667792492892 1106 50-51 -0.0867820316640362 1106 52-53 -0.027930728682449057 1106 54-55 -0.07461078527857978 1106 56-57 -0.11542607643860237 1106 58-59 -0.1631484008098667 1106 60-61 -0.16222979492012257 1106 62-63 -0.1679669935415049 1106 64-65 -0.17780431192095847 1106 66-67 -0.11013429036695754 1106 68-69 -0.11766681589732997 1106 70-71 -0.06690613534489387 1106 72-73 -0.10832864663851893 1106 74-75 -0.09626560492134217 1106 76-77 -0.11030796461108139 1106 78-79 -0.10371306233160027 1106 80-81 -0.08355723747279598 1106 82-83 -0.014330304179210884 1106 84-85 -0.08698610581468813 1106 86-87 -0.007017178327394902 1106 88-89 -0.050176658686318376 1106 90-91 -0.06143365513226229 1106 92-93 -0.10994764853544581 1106 94-95 -0.08150673881017667 1106 96-97 -0.10869788843030648 1106 98-99 -0.13688746434277732 1106 100 -0.149016283783034 1107 1 -0.05573554850870721 1107 2 -0.11400026912520644 1107 3 -0.0834416843249528 1107 4 -0.042590987147598014 1107 5 -0.03449624012801422 1107 6 -0.0781873642915869 1107 7 -0.05670708230970689 1107 8 -0.020836790150298157 1107 9 -0.013914477536630443 1107 10-11 0.019004966813184865 1107 12-13 -0.03280532336570019 1107 14-15 -0.05306954550316334 1107 16-17 -0.05597567187729169 1107 18-19 -0.130256220591221 1107 20-21 -0.10718598048002548 1107 22-23 -0.13963270422645024 1107 24-25 -0.1346026294218774 1107 26-27 -0.1667947453940073 1107 28-29 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0.028207470851903338 2214 32-33 0.006834035230419033 2214 34-35 0.015938830710453544 2214 36-37 -0.009796431553937168 2214 38-39 0.0631656937884415 2214 40-41 0.05925081614754646 2214 42-43 0.09245561507059818 2214 44-45 0.07463804314383538 2214 46-47 0.12587255065459857 2214 48-49 0.08183589994183649 2214 50-51 0.11984419243700728 2214 52-53 0.05447938247272077 2214 54-55 0.12381594100339299 2214 56-57 0.058933867066038204 2214 58-59 0.05832421253463593 2214 60-61 0.084784022061271 2214 62-63 0.1115052751140766 2214 64-65 0.0927373260800195 2214 66-67 0.10609204554779694 2214 68-69 0.08910474502503263 2214 70-71 0.12812918826335817 2214 72-73 0.1653798207988828 2214 74-75 0.08751173724686367 2214 76-77 0.10516744687883062 2214 78-79 0.096233412803862 2214 80-81 0.13328813732035627 2214 82-83 0.16129076640214635 2214 84-85 0.14337443739263733 2214 86-87 0.1914345589187647 2214 88-89 0.1274646952965952 2214 90-91 0.13672050768700927 2214 92-93 0.10890865681970041 2214 94-95 0.0575483021251344 2214 96-97 0.05348472803882487 2214 98-99 0.07006905160888977 2214 100 0.05562860956656834 2215 1 0.022737837410076622 2215 2 0.007533356349526343 2215 3 -0.029803121660627596 2215 4 0.0018444203896450517 2215 5 0.02018047620268959 2215 6 0.02235929068942255 2215 7 0.050176002703437916 2215 8 0.023191489136877408 2215 9 0.03564579123344913 2215 10-11 0.02558796418391296 2215 12-13 0.04907559980111387 2215 14-15 0.05963346355322585 2215 16-17 0.0454163234484497 2215 18-19 -0.006260661140437662 2215 20-21 0.031562485777641314 2215 22-23 0.043721546430866454 2215 24-25 0.0023829387931399992 2215 26-27 0.04360852658962955 2215 28-29 0.04310390984360879 2215 30-31 0.028073077563441018 2215 32-33 0.030498214385389133 2215 34-35 0.09210090912458213 2215 36-37 0.08958188447056159 2215 38-39 0.0875729330563999 2215 40-41 0.1265881322058604 2215 42-43 0.09261203738378043 2215 44-45 0.055924034822055546 2215 46-47 0.11263418930011682 2215 48-49 0.1226722992517395 2215 50-51 0.07351720755827529 2215 52-53 0.07381956717554061 2215 54-55 0.07368524177129387 2215 56-57 0.04785321350221494 2215 58-59 0.06269995083848556 2215 60-61 0.04326707743828706 2215 62-63 0.02669488304344725 2215 64-65 0.007727049025795907 2215 66-67 0.010828854180800818 2215 68-69 0.06525372510013483 2215 70-71 0.023479494409968993 2215 72-73 0.022456644992921326 2215 74-75 0.05164783548440255 2215 76-77 0.07370353158503207 2215 78-79 0.03136183200484055 2215 80-81 -0.0010089703553006757 2215 82-83 0.055936306133546054 2215 84-85 0.07576976907964905 2215 86-87 0.04872534069205159 2215 88-89 0.04983297783101648 2215 90-91 0.07093726625404173 2215 92-93 0.03936359406809942 2215 94-95 0.08416004056429216 2215 96-97 0.07192172272776531 2215 98-99 0.11367196084741238 2215 100 0.10077712281037066 2216 1 0.09761446396201023 2216 2 0.08634853432558032 2216 3 0.09930376558416043 2216 4 0.06457841207033965 2216 5 0.06039848020358107 2216 6 0.02875502307440314 2216 7 0.017936050968131667 2216 8 0.036289612529373017 2216 9 0.06137605510161137 2216 10-11 0.023569814118815202 2216 12-13 0.052462561983183775 2216 14-15 0.07966336226087378 2216 16-17 0.099002936346551 2216 18-19 0.04459988564773454 2216 20-21 0.05306961434588686 2216 22-23 0.04757746361880777 2216 24-25 0.07836663040715308 2216 26-27 0.10093407829953094 2216 28-29 0.13464595410741254 2216 30-31 0.08752141211397912 2216 32-33 0.10904460882225209 2216 34-35 0.04550345572224046 2216 36-37 0.04425844116569522 2216 38-39 0.05154869909964077 2216 40-41 0.09202682651888239 2216 42-43 0.046493471353237226 2216 44-45 9.474495491446078E-4 2216 46-47 0.03181462939791402 2216 48-49 0.016613733856253532 2216 50-51 0.0038361990802044943 2216 52-53 0.007032103294676517 2216 54-55 -0.003646330324095004 2216 56-57 -0.0706093781848196 2216 58-59 -0.056336203227502324 2216 60-61 -0.038889236663251836 2216 62-63 -0.043541677462066275 2216 64-65 -0.014909270156245213 2216 66-67 -0.04905691090985442 2216 68-69 -0.049905834167006446 2216 70-71 -0.0011429677244692016 2216 72-73 -0.04799469339973683 2216 74-75 -0.05393732947288754 2216 76-77 -0.03073801415528976 2216 78-79 -0.028525939880978513 2216 80-81 -0.05174092983835976 2216 82-83 -0.02999956532995185 2216 84-85 -0.06633098981914998 2216 86-87 -0.04707075367812763 2216 88-89 -0.06592082142596212 2216 90-91 -0.08739254672026675 2216 92-93 -0.058943940939677475 2216 94-95 -0.11588837654426953 2216 96-97 -0.07272654831659509 2216 98-99 -0.11736073848259565 2216 100 -0.13779327696100552 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 9.0 5 22.0 6 51.0 7 133.0 8 269.0 9 535.0 10 844.0 11 1047.0 12 1426.0 13 2015.0 14 2414.0 15 3373.0 16 4340.0 17 6715.0 18 8321.0 19 4808.0 20 3623.0 21 3430.0 22 3748.0 23 3795.0 24 4080.0 25 4767.0 26 5851.0 27 7560.0 28 9338.0 29 11965.0 30 15523.0 31 19851.0 32 26182.0 33 34881.0 34 50788.0 35 89889.0 36 203494.0 37 534588.0 38 873911.0 39 389180.0 40 2045.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.84220670897085 18.491352998397065 14.216060422120322 25.450379870511764 2 14.652638431738705 18.95542045087084 39.38778875414112 27.004152363249332 3 18.077749200686135 23.51578176429396 28.88991204870621 29.51655698631369 4 12.77621567447528 15.669121536395817 35.59977985407837 35.95488293505053 5 14.63960403420359 36.39501641074811 33.785362580923774 15.180016974124527 6 34.11552307913059 34.95375179092683 16.417375363271706 14.513349766670878 7 29.799363118705337 30.754770720592422 20.3295764332506 19.11628972745164 8 27.046339859903245 34.84665808640085 18.821319890441 19.285682163254904 9 26.233477072115118 15.841621479688456 19.482523251714778 38.44237819648165 10-11 25.807388346470724 25.625467917759547 26.86297654800472 21.704167187765012 12-13 26.21012799729315 22.618601473778437 27.370198495384003 23.80107203354441 14-15 23.295507352830953 24.30089750151511 24.723864631673173 27.679730513980765 16-17 23.375792086310906 27.647201170114123 25.211890449564528 23.765116294010447 18-19 23.518908350340393 26.513535333634568 27.20114869914747 22.76640761687757 20-21 24.16345620530963 25.623850283641314 27.20725196642989 23.005441544619167 22-23 23.986868338604985 25.398714673325294 27.16868360019959 23.44573338787013 24-25 23.79479744647863 25.493347438662163 27.072894426325 23.638960688534212 26-27 23.662538573719974 25.69747496054291 27.051543698322995 23.58844276741412 28-29 23.608401522176276 25.661840445602756 27.16774134139107 23.562016690829896 30-31 23.671134871485393 25.75969261514832 26.985904103704268 23.58326840966201 32-33 23.598331682233265 25.660450735328443 27.187878912475423 23.55333866996287 34-35 23.69456866382303 25.598840673067492 27.1707934245416 23.53579723856788 36-37 23.907319529145106 25.62881340360707 26.912085085733118 23.5517819815147 38-39 23.766551097196135 25.74739326242122 26.88352182078666 23.602533819595987 40-41 23.72102824422953 25.61187907035113 27.032752975581804 23.634339709837544 42-43 23.75746701919292 25.69688966821957 26.87593665663426 23.66970665595325 44-45 23.711891045347304 25.888208664709726 26.749278676117704 23.650621613825262 46-47 23.889011549804128 25.834958560717048 26.60934987406852 23.666680015410304 48-49 23.992398336097366 25.91363022710763 26.30105895615466 23.79291248064034 50-51 23.869400541469627 26.0248925262886 26.286525749788954 23.81918118245282 52-53 23.92157240664478 26.070867946024546 26.20456448507666 23.802995162254017 54-55 23.931302704072117 26.09969575467809 26.272644668575616 23.69635687267418 56-57 23.788316536236735 26.01859234184313 26.385329486287457 23.807761635632673 58-59 23.779655612313185 25.882850279565776 26.451747197757047 23.885746910363988 60-61 23.91897361438585 25.94191352988164 26.33310419116025 23.806008664572264 62-63 23.74072724881951 26.03985770520409 26.428253710653117 23.791161335323284 64-65 23.777481764486687 25.9536434619849 26.358957995142905 23.909916778385508 66-67 23.776952803348536 25.918229019992484 26.372281430258663 23.932536746400316 68-69 23.8434682716722 25.975715896202335 26.31770902935543 23.86310680277004 70-71 23.82423375647683 25.83989422055118 26.305656176528665 24.03021584644333 72-73 23.811648994286905 25.957797012263516 26.295533128805715 23.93502086464386 74-75 23.80444121595055 26.042567006715018 26.204960154465155 23.948031622869273 76-77 23.92027346462045 25.99706353615178 26.076878302504603 24.00578469672316 78-79 23.890045179197397 26.048954231248022 26.17986404481557 23.88113654473901 80-81 23.751881840745412 25.97700460207768 26.283238714844646 23.987874842332264 82-83 23.82962539103858 25.982907373968224 26.237617825534258 23.94984940945894 84-85 23.883939995961125 25.995718280228004 26.077309519256282 24.04303220455459 86-87 23.878766178086803 26.138601521162037 26.096113832740702 23.886518468010454 88-89 23.735471472325504 26.053097413357783 26.174777914881897 24.036653199434813 90-91 23.7314908248629 26.000369198402595 26.161647224501756 24.10649275223275 92-93 23.786919752159758 26.136510864586228 26.141115092638138 23.935454290615876 94-95 23.774120605866834 25.942268803348977 26.203040213020067 24.080570377764126 96-97 23.78087771408013 26.07812610420954 26.130078828515323 24.01091735319501 98-99 23.744879929021955 26.14103138739763 26.13190857572137 23.982180107859044 100 23.903474676921903 25.815666990890467 26.076202444142066 24.204655888045565 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 180.0 1 148.0 2 238.0 3 306.0 4 163.0 5 107.5 6 312.5 7 880.0 8 1465.0 9 1564.5 10 1158.0 11 742.5 12 688.0 13 801.0 14 929.0 15 1050.5 16 1158.5 17 1278.5 18 1446.0 19 1631.0 20 1861.0 21 2165.5 22 2566.5 23 3285.5 24 4298.5 25 5611.5 26 7524.0 27 9977.5 28 12983.0 29 16812.0 30 20549.0 31 24362.5 32 28532.5 33 32241.5 34 36097.0 35 40384.5 36 45263.0 37 50446.0 38 55705.0 39 60284.5 40 64502.0 41 68942.0 42 71765.5 43 73707.5 44 76611.5 45 78636.0 46 79813.5 47 79722.0 48 79014.5 49 80414.0 50 81531.0 51 81956.5 52 81122.0 53 80964.5 54 81402.0 55 78715.5 56 75375.5 57 70592.0 58 65138.5 59 61552.5 60 57129.0 61 50767.0 62 44727.5 63 39095.5 64 32939.5 65 27840.5 66 22670.5 67 17689.0 68 13854.5 69 10539.5 70 7905.5 71 5754.5 72 4301.0 73 3212.5 74 2410.0 75 1820.0 76 1251.5 77 860.5 78 606.0 79 404.5 80 231.0 81 100.5 82 52.0 83 19.5 84 11.5 85 10.0 86 4.5 87 3.0 88 4.5 89 4.0 90 3.5 91 3.5 92 1.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22858342095626422 2 0.0 3 0.0 4 0.0 5 0.02886738349719357 6 0.005567892959399353 7 0.0 8 0.0 9 0.010964466135432572 10-11 1.2848983752460045E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 9.422588085137366E-4 32-33 3.426395667322679E-4 34-35 5.139593500984018E-4 36-37 0.001734612806582106 38-39 0.0 40-41 8.351839439099029E-4 42-43 0.002055837400393607 44-45 0.0019487625357897734 46-47 5.78204268860702E-4 48-49 0.0026982865880166096 50-51 0.002484136858808942 52-53 0.002141497292076674 54-55 9.208438355929699E-4 56-57 0.0015632930232159722 58-59 0.0014348031856913717 60-61 8.565989168306697E-4 62-63 0.0036191304236095796 64-65 0.003640545396530346 66-67 0.0026768716150958427 68-69 0.005096763555142485 70-71 0.006253172092863889 72-73 1.7131978336613394E-4 74-75 0.0020986673462351406 76-77 0.0014562181586121384 78-79 6.424491876230022E-5 80-81 0.0 82-83 2.998096208907344E-4 84-85 2.3556470212843415E-4 86-87 8.994288626722031E-4 88-89 5.139593500984018E-4 90-91 0.001156408537721404 92-93 1.927347562869007E-4 94-95 4.925443771776351E-4 96-97 0.0 98-99 3.640545396530346E-4 100 3.426395667322679E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2334815.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.96486130105201 #Duplication Level Percentage of deduplicated Percentage of total 1 79.98430592251634 50.36200728672154 2 11.517730048725863 14.504245500419657 3 3.481791858573923 6.576916243627175 4 1.6048039281843642 4.041850270140477 5 0.8758055776455366 2.757248836156948 6 0.5715040198208243 2.159080280460713 7 0.37517567049948636 1.6536018839570545 8 0.2680468805024769 1.3502027722414494 9 0.2093076972391198 1.186112711331339 >10 1.049638510756922 11.548634471317945 >50 0.04826625943902616 2.042752172080499 >100 0.013215753038413991 1.3296954514314185 >500 2.0393652884162356E-4 0.08556276556732928 >1k 2.0393652884120457E-4 0.40208935454635175 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4056 0.17371826033325982 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3087 0.13221604281281385 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.2829945841533485E-5 0.0 0.0 0.0 6 0.0 4.2829945841533485E-5 0.0 0.0 0.0 7 0.0 4.2829945841533485E-5 0.0 0.0 0.0 8 0.0 4.2829945841533485E-5 0.0 0.0 0.0 9 0.0 4.2829945841533485E-5 0.0 0.0 0.0 10-11 0.0 4.2829945841533485E-5 0.0 0.0 0.0 12-13 0.0 4.2829945841533485E-5 0.0 0.0 0.0 14-15 0.0 4.2829945841533485E-5 0.0 0.0 0.0 16-17 0.0 4.2829945841533485E-5 0.0 0.0 0.0 18-19 0.0 8.565989168306697E-5 0.0 2.1414972920766743E-5 0.0 20-21 0.0 8.565989168306697E-5 0.0 4.2829945841533485E-5 0.0 22-23 0.0 8.565989168306697E-5 0.0 1.2848983752460045E-4 0.0 24-25 0.0 8.565989168306697E-5 0.0 2.141497292076674E-4 0.0 26-27 0.0 1.2848983752460045E-4 0.0 5.353743230191686E-4 0.0 28-29 0.0 1.2848983752460045E-4 0.0 0.0014348031856913717 0.0 30-31 0.0 1.2848983752460045E-4 0.0 0.004518559286281782 0.0 32-33 0.0 1.2848983752460045E-4 0.0 0.011200030837561006 0.0 34-35 0.0 1.2848983752460045E-4 0.0 0.02184327237918208 0.0 36-37 0.0 1.2848983752460045E-4 0.0 0.03674809353203573 0.0 38-39 0.0 1.499048104453672E-4 0.0 0.05655694348374496 0.0 40-41 0.0 1.7131978336613394E-4 0.0 0.07413863625169445 0.0 42-43 0.0 1.7131978336613394E-4 0.0 0.09144193437167399 0.0 44-45 0.0 1.7131978336613394E-4 0.0 0.11238577788818387 0.0 46-47 0.0 1.7131978336613394E-4 0.0 0.13834072506815315 0.0 48-49 0.0 1.7131978336613394E-4 0.0 0.16292511398119336 0.0 50-51 0.0 1.7131978336613394E-4 0.0 0.18639592430235372 0.0 52-53 0.0 1.7131978336613394E-4 0.0 0.2082391966815358 0.0 54-55 0.0 1.7131978336613394E-4 0.0 0.23275934067581372 0.0 56-57 0.0 1.7131978336613394E-4 0.0 0.2613911594708789 0.0 58-59 0.0 1.7131978336613394E-4 0.0 0.2904512777243593 0.0 60-61 0.0 1.7131978336613394E-4 0.0 0.32015384516546275 0.0 62-63 0.0 1.7131978336613394E-4 0.0 0.3487000040688448 0.0 64-65 0.0 1.7131978336613394E-4 0.0 0.37525457049059563 0.0 66-67 0.0 2.569796750492009E-4 0.0 0.4076554245197157 0.0 68-69 0.0 2.7839464796996767E-4 0.0 0.4430543747577431 0.0 70-71 0.0 2.998096208907344E-4 0.0 0.4788602094812651 0.0 72-73 0.0 2.998096208907344E-4 0.0 0.5131669961003333 0.0 74-75 0.0 2.998096208907344E-4 0.0 0.5490370757426177 0.0 76-77 0.0 3.426395667322679E-4 0.0 0.5865989382456426 0.0 78-79 0.0 3.426395667322679E-4 0.0 0.6280797407931678 0.0 80-81 0.0 3.426395667322679E-4 0.0 0.6693249786385644 0.0 82-83 0.0 3.426395667322679E-4 0.0 0.712047849615494 0.0 84-85 0.0 3.6405453965303466E-4 0.0 0.7563340136156398 0.0 86-87 0.0 3.854695125738014E-4 0.0 0.8004060278865778 0.0 88 0.0 3.854695125738014E-4 0.0 0.8375395909311872 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2910 0.0 47.76196 1 GTATCAA 5015 0.0 41.61845 1 TCAACGC 6240 0.0 32.62728 4 ATCAACG 6265 0.0 32.422035 3 CAACGCA 6605 0.0 30.82426 5 TATCAAC 6730 0.0 30.38952 2 AACGCAG 6860 0.0 29.807241 6 ACGCAGA 8140 0.0 25.0014 7 GTACATG 7920 0.0 24.925392 1 TACATGG 8150 0.0 23.940882 2 CGCAGAG 8530 0.0 23.858313 8 ACATGGG 8470 0.0 21.927608 3 GCAGAGT 9725 0.0 20.733305 9 GTGGTAT 1655 0.0 20.212194 1 AGAGTAC 8600 0.0 20.137798 10-11 CATGGGG 7250 0.0 19.586035 4 CAGAGTA 8960 0.0 18.804167 10-11 GAGTACT 5090 0.0 18.697355 12-13 AGTACTT 5515 0.0 17.299097 12-13 GTACTTT 5565 0.0 17.016993 14-15 >>END_MODULE