FastQCFastQC Report
Wed 25 May 2016
SRR2049389_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049389_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5922853
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55300.044.6370051
GTATCAA94300.034.602591
TCAACGC99400.031.7365934
ATCAACG100650.031.3424433
CAACGCA106500.029.5327825
AACGCAG113500.027.7474046
TATCAAC118650.026.9045772
ACGCAGA132800.023.254117
GTACATG152450.023.0076481
CGCAGAG134900.022.4391488
TACATGG151750.021.4698332
ACATGGG158550.019.8670183
GTGGTAT36350.018.1085831
GCAGAGT156900.018.0946469
CATGGGG113550.017.7620984
GAGTACA117800.016.963071
AGAGTAC132700.016.93140810-11
CAGAGTA148750.016.11570410-11
TGGTATC36750.015.0955462
CTGTCGC13950.014.8256789
Produced by FastQC (version 0.11.5)