##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049389_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5922853
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.919399654186925	31.0	31.0	34.0	28.0	34.0
2	31.11026138923252	31.0	31.0	34.0	28.0	34.0
3	31.17418497470729	31.0	31.0	34.0	28.0	34.0
4	34.856190251556136	37.0	35.0	37.0	32.0	37.0
5	34.870674825122286	37.0	35.0	37.0	32.0	37.0
6	34.973706083875456	37.0	35.0	37.0	32.0	37.0
7	34.94632367205467	37.0	35.0	37.0	32.0	37.0
8	34.9067037456442	37.0	35.0	37.0	32.0	37.0
9	36.54657628679962	39.0	37.0	39.0	32.0	39.0
10-11	36.504544769218484	39.0	37.0	39.0	32.0	39.0
12-13	36.44457443059957	39.0	37.0	39.0	32.0	39.0
14-15	37.684318266889285	40.0	38.0	41.0	32.0	41.0
16-17	37.6536817645145	40.0	38.0	41.0	32.0	41.0
18-19	37.68246670987782	40.0	38.0	41.0	32.0	41.0
20-21	37.75370889670907	40.0	38.0	41.0	32.0	41.0
22-23	37.71049036672022	40.0	38.0	41.0	32.0	41.0
24-25	37.71445247754756	40.0	38.0	41.0	32.0	41.0
26-27	37.701490143348146	40.0	38.0	41.0	32.0	41.0
28-29	37.64333995795607	40.0	38.0	41.0	32.0	41.0
30-31	37.582542399752285	40.0	38.0	41.0	32.0	41.0
32-33	37.52983283562837	40.0	38.0	41.0	32.0	41.0
34-35	37.431440641866345	40.0	38.0	41.0	32.0	41.0
36-37	37.358667604953226	40.0	37.5	41.0	32.0	41.0
38-39	37.259639991065114	40.0	37.0	41.0	31.0	41.0
40-41	37.158053475242426	40.0	37.0	41.0	31.0	41.0
42-43	37.02789652216592	40.0	37.0	41.0	31.0	41.0
44-45	36.917327426495305	40.0	37.0	41.0	31.0	41.0
46-47	36.803429191978935	40.0	37.0	41.0	30.0	41.0
48-49	36.67620444066398	40.0	36.0	41.0	30.0	41.0
50-51	36.53800271592086	40.0	36.0	41.0	30.0	41.0
52-53	36.39725601834117	40.0	36.0	41.0	30.0	41.0
54-55	36.258515955064226	39.0	35.0	41.0	30.0	41.0
56-57	36.130559883893795	39.0	35.0	41.0	29.5	41.0
58-59	35.92586545706942	39.0	35.0	41.0	29.0	41.0
60-61	35.70886192853343	39.0	35.0	41.0	29.0	41.0
62-63	35.50057396325724	38.5	35.0	40.0	28.0	41.0
64-65	35.25354394242099	38.0	35.0	40.0	28.0	41.0
66-67	34.99020548036563	37.5	34.0	40.0	28.0	41.0
68-69	34.703956269048035	37.0	34.0	40.0	28.0	41.0
70-71	34.41812391764577	36.5	34.0	39.5	28.0	41.0
72-73	34.07187743811977	36.0	34.0	39.0	27.0	41.0
74-75	33.718893833765584	36.0	34.0	39.0	26.0	40.5
76-77	33.37819054432045	35.0	33.5	38.5	26.0	40.0
78-79	33.05539019793333	35.0	33.0	37.0	26.0	39.0
80-81	32.69213873786839	35.0	33.0	37.0	26.0	39.0
82-83	32.37241461167447	35.0	33.0	36.0	25.5	39.0
84-85	32.07361747792829	35.0	33.0	36.0	25.0	37.0
86-87	31.747607360844512	35.0	33.0	36.0	24.5	37.0
88-89	31.489859701059608	35.0	33.0	35.0	24.0	37.0
90-91	31.278551738494944	35.0	32.5	35.0	23.5	36.0
92-93	31.03992172353425	35.0	32.0	35.0	21.0	36.0
94-95	30.840086947962412	35.0	32.0	35.0	19.5	36.0
96-97	30.590272036128535	35.0	32.0	35.0	17.0	36.0
98-99	30.383280827668692	35.0	32.0	35.0	2.0	35.0
100	30.26939550922503	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.31455761128563253
1101	2	0.35305256765182236
1101	3	0.3367851442415457
1101	4	0.45027197515020134
1101	5	0.3919916710846394
1101	6	0.5080448893194855
1101	7	0.47927924886043627
1101	8	0.4810199089104046
1101	9	0.6677413727438406
1101	10-11	0.6259234833887888
1101	12-13	0.6417038971106805
1101	14-15	0.7245421844185671
1101	16-17	0.7466475927900618
1101	18-19	0.7446314297242509
1101	20-21	0.7041645523519904
1101	22-23	0.7200060709859386
1101	24-25	0.7804469942620216
1101	26-27	0.6644028794977004
1101	28-29	0.6551261081399105
1101	30-31	0.6480364623230059
1101	32-33	0.6716087070596188
1101	34-35	0.7100773420328963
1101	36-37	0.6583743616358575
1101	38-39	0.7067103990770676
1101	40-41	0.7581452294994193
1101	42-43	0.7081914272576455
1101	44-45	0.7083741389222737
1101	46-47	0.7107760067400122
1101	48-49	0.6267269345796578
1101	50-51	0.6758950147968505
1101	52-53	0.6775071645186586
1101	54-55	0.7069612613017142
1101	56-57	0.6639362384678762
1101	58-59	0.7387027347227644
1101	60-61	0.7058293374346007
1101	62-63	0.647310422216691
1101	64-65	0.655584388161401
1101	66-67	0.750058344644934
1101	68-69	0.7019628485368727
1101	70-71	0.7207234291967168
1101	72-73	0.7507894185443718
1101	74-75	0.7331640097768499
1101	76-77	0.7452431034495959
1101	78-79	0.703744937854168
1101	80-81	0.716179808530093
1101	82-83	0.7564170584553764
1101	84-85	0.6850237767062382
1101	86-87	0.7244037480222651
1101	88-89	0.7260723605416146
1101	90-91	0.7586275981835549
1101	92-93	0.7087539760260668
1101	94-95	0.7470034056478134
1101	96-97	0.732910535463084
1101	98-99	0.7968650181379715
1101	100	0.8093131467447954
1102	1	0.13875316861773257
1102	2	0.23149455069536984
1102	3	0.20924856522563573
1102	4	0.307500999903219
1102	5	0.27063665958431926
1102	6	0.4283390151939699
1102	7	0.49684338459795185
1102	8	0.4583108416625805
1102	9	0.5964057431499583
1102	10-11	0.5307420204048299
1102	12-13	0.5474599138445768
1102	14-15	0.620351677608241
1102	16-17	0.6503178615038365
1102	18-19	0.6266663271550428
1102	20-21	0.579001413382457
1102	22-23	0.5929854788682363
1102	24-25	0.626925678520422
1102	26-27	0.625074594293693
1102	28-29	0.6602741145610054
1102	30-31	0.6342005447150285
1102	32-33	0.6788085684693286
1102	34-35	0.669558445241563
1102	36-37	0.6428948697111068
1102	38-39	0.6368582107282492
1102	40-41	0.6638402617809191
1102	42-43	0.6567779758894545
1102	44-45	0.6040507821637959
1102	46-47	0.5197880227245193
1102	48-49	0.576180669395427
1102	50-51	0.62145514575829
1102	52-53	0.6288298086339807
1102	54-55	0.6295253537911023
1102	56-57	0.581255200297015
1102	58-59	0.6855380564881699
1102	60-61	0.6640703084230282
1102	62-63	0.6474781007053636
1102	64-65	0.6498147973450585
1102	66-67	0.6700494269435993
1102	68-69	0.7160326433984565
1102	70-71	0.7141341714534946
1102	72-73	0.7478225897309798
1102	74-75	0.6909888556202972
1102	76-77	0.6915431453604057
1102	78-79	0.6411368405543527
1102	80-81	0.601251789967634
1102	82-83	0.6195424201599664
1102	84-85	0.6074396216892737
1102	86-87	0.6002754507210533
1102	88-89	0.5953295237494771
1102	90-91	0.6399241719630773
1102	92-93	0.6298713344970466
1102	94-95	0.6408757280523112
1102	96-97	0.5657680627771846
1102	98-99	0.6471988660319354
1102	100	0.6537652961028257
1103	1	0.06608864043379725
1103	2	0.1577648130504521
1103	3	0.13558165240883113
1103	4	0.20608284052874382
1103	5	0.17624052641095744
1103	6	0.2754852465611819
1103	7	0.323432868779733
1103	8	0.32215797007099667
1103	9	0.36245543920661305
1103	10-11	0.37125956932774784
1103	12-13	0.3861390386654264
1103	14-15	0.4696930326612403
1103	16-17	0.44407757669095105
1103	18-19	0.4377726681519505
1103	20-21	0.4011041103935966
1103	22-23	0.39399227333477427
1103	24-25	0.4363974372810162
1103	26-27	0.425786450165063
1103	28-29	0.40856437132931944
1103	30-31	0.45795553762024355
1103	32-33	0.496810622933026
1103	34-35	0.46629839044187094
1103	36-37	0.47014197434780414
1103	38-39	0.47780810114402783
1103	40-41	0.4776353261714803
1103	42-43	0.46484328042562595
1103	44-45	0.38357123038477425
1103	46-47	0.4644431964649698
1103	48-49	0.5108592793762128
1103	50-51	0.4742906965025071
1103	52-53	0.5044412832252334
1103	54-55	0.5302261661331826
1103	56-57	0.42844620036569125
1103	58-59	0.5145124868559918
1103	60-61	0.4818092374670826
1103	62-63	0.47024751910047513
1103	64-65	0.4493208639121633
1103	66-67	0.5310783859978372
1103	68-69	0.5042077108225698
1103	70-71	0.46670045128282567
1103	72-73	0.5005895271938812
1103	74-75	0.4923474895266864
1103	76-77	0.5272396574588498
1103	78-79	0.4630122912288641
1103	80-81	0.42830577857329644
1103	82-83	0.40957921414500476
1103	84-85	0.4956858381219291
1103	86-87	0.4746839194787391
1103	88-89	0.4574574072356654
1103	90-91	0.48789336340185585
1103	92-93	0.48101467350302585
1103	94-95	0.5156727314289675
1103	96-97	0.5444176329685071
1103	98-99	0.5420316048003855
1103	100	0.49570170099655897
1104	1	0.07899718724905824
1104	2	0.16731595494101015
1104	3	0.16695986192658907
1104	4	0.21621887983859267
1104	5	0.21815077684506434
1104	6	0.3505529084954375
1104	7	0.4100890813642408
1104	8	0.40491796355620124
1104	9	0.551701707969805
1104	10-11	0.47605678271818874
1104	12-13	0.4123640165697964
1104	14-15	0.5740610846350904
1104	16-17	0.5632760521805764
1104	18-19	0.5039507301978006
1104	20-21	0.4915187817106812
1104	22-23	0.5257205666342983
1104	24-25	0.568368374096309
1104	26-27	0.5200011213029399
1104	28-29	0.5111050082940523
1104	30-31	0.49447588872135384
1104	32-33	0.5010230147216532
1104	34-35	0.5130646541707833
1104	36-37	0.4362196540460914
1104	38-39	0.5629382938920529
1104	40-41	0.5313585814681829
1104	42-43	0.5337187837761803
1104	44-45	0.499585399156004
1104	46-47	0.4658489284183176
1104	48-49	0.4467867446523215
1104	50-51	0.5296370249064921
1104	52-53	0.5570235818969067
1104	54-55	0.5577994821562342
1104	56-57	0.5263332429303844
1104	58-59	0.6681987156509663
1104	60-61	0.5621289901201223
1104	62-63	0.5490969777670927
1104	64-65	0.5575573995715004
1104	66-67	0.6010053882358974
1104	68-69	0.6134792722981004
1104	70-71	0.5986668528341355
1104	72-73	0.5673499571120999
1104	74-75	0.6414193491303521
1104	76-77	0.6850296040192418
1104	78-79	0.5805009584105889
1104	80-81	0.6060379908095896
1104	82-83	0.5346162079073693
1104	84-85	0.542017964050352
1104	86-87	0.5368604588338393
1104	88-89	0.5191090690480564
1104	90-91	0.5295139221257017
1104	92-93	0.5148956388139752
1104	94-95	0.5248717493582191
1104	96-97	0.5391392680455915
1104	98-99	0.5484022997716949
1104	100	0.4953672769712938
1105	1	0.0816051208859605
1105	2	0.11114991204682667
1105	3	0.14233104949829212
1105	4	0.22897502003656456
1105	5	0.14297146503754732
1105	6	0.26595551725412037
1105	7	0.26932476979801834
1105	8	0.35215976990842535
1105	9	0.3866604729821361
1105	10-11	0.355581216577292
1105	12-13	0.29389024968573096
1105	14-15	0.386880615928618
1105	16-17	0.3607315554751622
1105	18-19	0.3332594765254413
1105	20-21	0.35013374684164233
1105	22-23	0.34947564140874476
1105	24-25	0.2724532462674176
1105	26-27	0.29082398958335887
1105	28-29	0.3604340586304886
1105	30-31	0.30169892460414616
1105	32-33	0.39198016133266833
1105	34-35	0.43369951982337085
1105	36-37	0.3355580974748307
1105	38-39	0.35533441378863984
1105	40-41	0.4178618111662473
1105	42-43	0.4005498595697574
1105	44-45	0.3613980099439118
1105	46-47	0.3132734629920719
1105	48-49	0.3482130213844101
1105	50-51	0.3582675303831948
1105	52-53	0.3459771940336367
1105	54-55	0.3673556587086537
1105	56-57	0.3223337500067558
1105	58-59	0.3501121445382438
1105	60-61	0.3450474307738247
1105	62-63	0.33778500995911287
1105	64-65	0.33528317248156014
1105	66-67	0.31292007237295394
1105	68-69	0.2980247772630875
1105	70-71	0.33114449259264234
1105	72-73	0.3218806590350667
1105	74-75	0.3423167512216949
1105	76-77	0.44093504998944866
1105	78-79	0.3242804493318303
1105	80-81	0.35503627595637255
1105	82-83	0.358280704329907
1105	84-85	0.4099347032087266
1105	86-87	0.40377039207642085
1105	88-89	0.352084113805212
1105	90-91	0.39777424743903467
1105	92-93	0.4111421604395993
1105	94-95	0.4254302274638029
1105	96-97	0.45443010260727235
1105	98-99	0.4448447045316648
1105	100	0.4252116489178448
1106	1	0.12416039976147886
1106	2	0.1461931507869565
1106	3	0.1353486184699193
1106	4	0.1660977609853731
1106	5	0.17179943372118345
1106	6	0.24936179848972273
1106	7	0.24354366308966746
1106	8	0.2769720874912309
1106	9	0.33042676040066965
1106	10-11	0.3363363405760822
1106	12-13	0.33748562119382086
1106	14-15	0.40560914550282234
1106	16-17	0.4565351726892999
1106	18-19	0.385803336973467
1106	20-21	0.37235324527075164
1106	22-23	0.36382672763298984
1106	24-25	0.32338868579276436
1106	26-27	0.30290768879463315
1106	28-29	0.360133855307339
1106	30-31	0.3564119573976896
1106	32-33	0.4064310686720063
1106	34-35	0.4098716826913531
1106	36-37	0.31411701780034207
1106	38-39	0.3335524636320386
1106	40-41	0.40953448620010136
1106	42-43	0.37483455396902343
1106	44-45	0.3251681945791347
1106	46-47	0.3488531034564417
1106	48-49	0.38941190188013763
1106	50-51	0.4529394178782269
1106	52-53	0.4651687600063923
1106	54-55	0.42114975917512965
1106	56-57	0.3317241005943501
1106	58-59	0.438158940737722
1106	60-61	0.46998105412787794
1106	62-63	0.38597763618731307
1106	64-65	0.43908453991004137
1106	66-67	0.5214064989022589
1106	68-69	0.4942059918943542
1106	70-71	0.44286639323019017
1106	72-73	0.4240958238502657
1106	74-75	0.48036563453818815
1106	76-77	0.48069322522682967
1106	78-79	0.3770483586300202
1106	80-81	0.4472792841775899
1106	82-83	0.37213361469442674
1106	84-85	0.3392915916182986
1106	86-87	0.3974286617020901
1106	88-89	0.40061558031988653
1106	90-91	0.39283184680920513
1106	92-93	0.4089432624879876
1106	94-95	0.3945172921196516
1106	96-97	0.3928480957316758
1106	98-99	0.4116332714816977
1106	100	0.4354890305650265
1107	1	0.08520594699284345
1107	2	0.07152296953505655
1107	3	0.092193265821642
1107	4	0.1384216831718419
1107	5	0.11934885542066809
1107	6	0.20023002519081956
1107	7	0.2028939919600603
1107	8	0.24272441515265086
1107	9	0.30040134444157474
1107	10-11	0.25026895650837844
1107	12-13	0.2971503364626642
1107	14-15	0.30044590991673203
1107	16-17	0.3059469205870258
1107	18-19	0.2967676334381366
1107	20-21	0.28840636211272397
1107	22-23	0.28680936359162246
1107	24-25	0.2619126462090975
1107	26-27	0.28751480063224477
1107	28-29	0.34292243778259746
1107	30-31	0.3069632728546239
1107	32-33	0.2970775621909212
1107	34-35	0.24232007607660933
1107	36-37	0.2621027328391108
1107	38-39	0.3159128076416451
1107	40-41	0.38188926076956164
1107	42-43	0.3660221034498363
1107	44-45	0.2570023237931025
1107	46-47	0.2905079095064096
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2209	6	0.15838743806008182
2209	7	0.1743213163193289
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2209	9	0.1812104676148394
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2212	58-59	-0.4238897132374717
2212	60-61	-0.3680967234532986
2212	62-63	-0.30060980971604323
2212	64-65	-0.3349642574306273
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2214	4	-0.08321332391943059
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2214	92-93	-0.16375699926708265
2214	94-95	-0.11868933534070791
2214	96-97	-0.1925645773917175
2214	98-99	-0.1467551413162944
2214	100	-0.18142460444752473
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2215	3	-0.2968898476712667
2215	4	-0.27241522105450855
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2215	7	-0.48799914081681806
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2215	14-15	-0.711766091615516
2215	16-17	-0.5723221176903266
2215	18-19	-0.5995971674399385
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2215	22-23	-0.5588088453407423
2215	24-25	-0.6494101394299818
2215	26-27	-0.5584077880279921
2215	28-29	-0.5358361259649485
2215	30-31	-0.5381590149148323
2215	32-33	-0.497649503641199
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2215	36-37	-0.5870126238692777
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2215	62-63	-0.3178645010680441
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2215	80-81	-0.3781644519698162
2215	82-83	-0.36812426471433213
2215	84-85	-0.3189167176470349
2215	86-87	-0.21122640570125384
2215	88-89	-0.2223440579737428
2215	90-91	-0.23097632327187867
2215	92-93	-0.27443483947743275
2215	94-95	-0.3454519871873991
2215	96-97	-0.2831365119750906
2215	98-99	-0.41236366508326583
2215	100	-0.3726033853610353
2216	1	-1.0769887050520168
2216	2	-1.0966446933304361
2216	3	-1.1237980832474292
2216	4	-1.3708551529346664
2216	5	-1.2662152902214885
2216	6	-2.739202915827285
2216	7	-2.714730090719108
2216	8	-3.563479920288959
2216	9	-3.017230884697881
2216	10-11	-2.2461582325424274
2216	12-13	-1.9947905489878721
2216	14-15	-2.2260853360059514
2216	16-17	-2.590861583794748
2216	18-19	-2.2397118018684026
2216	20-21	-2.1301895889222564
2216	22-23	-2.2701334354057963
2216	24-25	-2.4103338229865017
2216	26-27	-2.4494675261231436
2216	28-29	-2.903134620983657
2216	30-31	-2.517072498818827
2216	32-33	-2.632133313725646
2216	34-35	-3.5868985760065826
2216	36-37	-2.5905859475671917
2216	38-39	-2.5069247700192747
2216	40-41	-3.021357746223103
2216	42-43	-2.850634282122556
2216	44-45	-2.622532765995487
2216	46-47	-2.4679191135657135
2216	48-49	-2.660314928489157
2216	50-51	-2.97870660340444
2216	52-53	-3.3022209929792297
2216	54-55	-2.698474640880633
2216	56-57	-2.5945431675101887
2216	58-59	-3.684765235604388
2216	60-61	-4.4244656031122815
2216	62-63	-3.4743664646099575
2216	64-65	-3.4169897502804183
2216	66-67	-3.0507582459513287
2216	68-69	-3.4401657511469494
2216	70-71	-3.2161311107362494
2216	72-73	-3.6532652844948466
2216	74-75	-4.043996748377257
2216	76-77	-4.691211792730009
2216	78-79	-3.663193876116619
2216	80-81	-3.540120393196794
2216	82-83	-3.8011321066659107
2216	84-85	-3.760671731292767
2216	86-87	-3.4401716609235287
2216	88-89	-3.4273947622872782
2216	90-91	-3.642955532289168
2216	92-93	-3.6956428672659314
2216	94-95	-3.5525233148944686
2216	96-97	-3.913487912871677
2216	98-99	-3.6252983995994974
2216	100	-3.267872980600739
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	73031.0
3	12172.0
4	8137.0
5	7039.0
6	6499.0
7	6953.0
8	7654.0
9	8791.0
10	10082.0
11	11403.0
12	12921.0
13	14212.0
14	16613.0
15	17519.0
16	17890.0
17	18848.0
18	17479.0
19	16450.0
20	16484.0
21	17031.0
22	19272.0
23	22900.0
24	27952.0
25	34656.0
26	42449.0
27	50192.0
28	62004.0
29	78946.0
30	96998.0
31	116282.0
32	149024.0
33	199050.0
34	275301.0
35	401418.0
36	665843.0
37	1182135.0
38	1580647.0
39	599015.0
40	1561.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.27298848682183	20.35667767542453	14.170123896232283	23.200209941521358
2	15.485473332022584	20.147107807665986	40.673606198621556	23.69381266168988
3	18.3566519204685	25.103671009447364	30.372556869143963	26.167120200940175
4	12.615933472860597	16.873981206049336	38.951518499186655	31.558566821903415
5	13.122957485287476	37.73768173376538	35.70284466364156	13.436516117305592
6	30.985239823102876	37.88168378954844	17.729104022921423	13.403972364427267
7	26.88269231808698	32.49796628217464	22.687929715793	17.931411683945385
8	24.529300063882697	36.62757131231139	20.654583118955255	18.18854550485066
9	24.55027808116396	17.11718529093712	21.864327877336866	36.468208750562056
10-11	23.65684972792536	27.07299981917714	29.181135944248787	20.089014508648713
12-13	24.057544047887102	24.646479251754744	29.104991798267964	22.19098490209019
14-15	21.54680272500676	26.95745950035012	26.245541645471597	25.25019612917153
16-17	21.122285019742623	30.236490908866724	26.92689680613211	21.71432726525854
18-19	21.05075976649595	29.144789738253856	28.915212896774456	20.889237598475738
20-21	22.106375658055537	28.43380122200239	28.476180952836206	20.983642167105863
22-23	21.79692733665011	28.321801640010502	28.473265964055983	21.40800505928341
24-25	21.656862351810993	28.42368490504291	28.432797407286202	21.48665533585989
26-27	21.403448350705258	28.355120481647894	28.528629999419806	21.712801168227042
28-29	21.448827731397962	28.406844527449632	28.635546082814457	21.50878165833795
30-31	21.724041799016735	28.726068780906488	28.2259740656784	21.32391535439837
32-33	21.3889143188781	28.48854316114772	28.593645963427434	21.52889655654674
34-35	21.560190034232445	28.2028330146897	28.63715281736543	21.599824133712428
36-37	21.837048094532808	28.518677850051237	28.138038759450318	21.50623529596564
38-39	21.64673027630848	28.680102325151672	28.26781719264219	21.405350205897655
40-41	21.536119725771446	28.398547546028073	28.46348334581265	21.60184938238783
42-43	21.484314332720576	28.38592010501843	28.417074649887187	21.712690912373812
44-45	21.59435241467513	28.462703114644118	28.327056887301648	21.615887583379106
46-47	21.575156645400586	28.60113831031983	28.18601269208828	21.637692352191305
48-49	21.52977169350045	28.44917052965186	28.243321999207684	21.77773577764001
50-51	21.582665522286074	28.351390151328655	28.194402370938587	21.871541955446684
52-53	21.5424222229898	28.372724294630885	28.24868915198794	21.83616433039138
54-55	21.647913910729947	28.67985958066072	28.040949560108587	21.631276948500748
56-57	21.506709382596277	28.45192701359172	28.138870910424913	21.902492693387092
58-59	21.4146237568192	28.131342791497953	28.407404385941355	22.04662906574149
60-61	21.660500247988246	28.445826876757568	28.086060239904665	21.807612635349518
62-63	21.49749266547648	28.493361348370023	28.0953969425551	21.9137490435984
64-65	21.551812225787238	28.219334067804542	28.23449245905925	21.99436124734897
66-67	21.452742459215706	28.282793735628413	28.094574449012278	22.169889356143603
68-69	21.772901062155352	28.39529704019645	28.021489699608004	21.810312198040197
70-71	21.743264490636843	28.216199070398112	28.113684105273805	21.926852333691237
72-73	21.552020360722512	28.32652247077955	28.240571414915	21.880885753582934
74-75	21.648045258871125	28.29079729981705	28.16910733443409	21.89205010687774
76-77	21.687699025692492	28.324681229540065	27.97255332883613	22.01506641593131
78-79	21.714466388101325	28.374235214168746	28.078560391616286	21.83273800611364
80-81	21.644983112618743	28.213935429518134	28.200178593958512	21.94090286390461
82-83	21.55912951495839	28.28682856733163	28.2627523923746	21.89128952533538
84-85	21.652218516962147	28.13245265096495	28.113012421479038	22.10231641059386
86-87	21.785007953901236	28.255304142033022	28.048993363008417	21.910694541057325
88-89	21.66095810877298	28.128912687355072	28.148657164432983	22.06147203943896
90-91	21.61126867232716	28.003658058821983	28.19973528139294	22.185337987457913
92-93	21.7320580053914	28.183471772167113	28.024014962355544	22.06045526008595
94-95	21.680580991538577	27.991042180089742	28.132935695939477	22.195441132432205
96-97	21.73030463528303	27.98053573168201	28.127829611844156	22.1613300211908
98-99	21.746369528333727	28.02629070821106	28.051515038445157	22.17582472501006
100	21.76272144522243	27.931589725424555	28.073801595278493	22.231887234074524
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1847.0
1	1441.0
2	1274.5
3	1583.0
4	1565.0
5	1310.5
6	1050.5
7	965.5
8	991.5
9	992.5
10	877.5
11	787.5
12	821.0
13	913.5
14	1019.0
15	1131.0
16	1423.0
17	1898.5
18	2524.5
19	3521.5
20	5106.0
21	7549.5
22	10823.0
23	15796.5
24	22645.0
25	31362.0
26	43399.5
27	57865.0
28	74512.0
29	93808.0
30	112188.0
31	127914.5
32	141190.0
33	153502.5
34	166231.0
35	177482.0
36	187160.5
37	197185.0
38	205764.5
39	209753.5
40	212012.5
41	213475.5
42	213069.5
43	211641.5
44	207620.5
45	203070.0
46	199209.5
47	192378.0
48	185026.5
49	180202.5
50	176091.5
51	169222.5
52	159113.0
53	149885.0
54	142040.0
55	135053.0
56	125876.5
57	114389.0
58	104309.0
59	95380.0
60	84386.5
61	71961.5
62	61634.0
63	52003.0
64	42669.5
65	34943.0
66	28203.0
67	21918.5
68	16967.5
69	13263.0
70	10141.0
71	7573.5
72	5668.0
73	4080.5
74	2902.5
75	2093.5
76	1426.0
77	999.5
78	745.0
79	510.5
80	340.5
81	225.0
82	176.0
83	134.0
84	91.0
85	76.0
86	55.5
87	44.5
88	45.5
89	38.0
90	29.0
91	27.5
92	21.5
93	14.5
94	11.0
95	9.0
96	12.0
97	15.0
98	13.0
99	17.0
100	30.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02032804123283154
2	0.04337436704912312
3	0.04529911513927494
4	0.048338191071093606
5	0.051782477127154766
6	0.04730828200531061
7	0.044927672525385993
8	0.0443536248493758
9	0.04455622991149705
10-11	0.0454932783238078
12-13	0.0466329317982398
14-15	0.044699741830499594
16-17	0.040757384996723704
18-19	0.04411725227690101
20-21	0.04339125080429989
22-23	0.04469129995291121
24-25	0.040782710629488864
26-27	0.04100219944678688
28-29	0.041761968429741544
30-31	0.041778852184918315
32-33	0.041517153979678374
34-35	0.041297665162380356
36-37	0.03856249682374356
38-39	0.039077451356635054
40-41	0.04035217487248122
42-43	0.03998917413618066
44-45	0.03891705568245574
46-47	0.0377436346976702
48-49	0.03777740220802374
50-51	0.03931382392910984
52-53	0.0397359178085291
54-55	0.038849520661748654
56-57	0.03695854008195037
58-59	0.03805598416844044
60-61	0.03730465706307416
62-63	0.03753258775796056
64-65	0.034442860560611584
66-67	0.035447443993629424
68-69	0.036401376161116945
70-71	0.03805598416844044
72-73	0.03446818619337674
74-75	0.034932489460737924
76-77	0.03604681730240477
78-79	0.036460469304235646
80-81	0.03646891118182403
82-83	0.03481430317450053
84-85	0.03614811983346539
86-87	0.03669684187671043
88-89	0.03731309894066255
90-91	0.03759168090107926
92-93	0.035920189138578994
94-95	0.03741440147172317
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5922853.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.092858843727484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.55562333357791	48.30113136679002
2	14.318347812074236	18.06770994765188
3	4.310890108769687	8.159591433702808
4	1.743302712974952	4.399598079664633
5	0.9128428222610494	2.87969316657131
6	0.5295095794379122	2.004496389112662
7	0.31446196496473217	1.3888213057068717
8	0.22600569348553132	1.1407476253569093
9	0.1819781702472706	1.0333370707245766
>10	0.8508747349628174	9.397579000450634
>50	0.04253676525939347	1.81434652468748
>100	0.013465608028512616	1.3099746448218579
>500	1.07130820777999E-4	0.045015854392550556
>1k	5.356313531263144E-5	0.05795759036595987
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.6883755176770385E-5	0.0	0.0	0.0
3	0.0	1.6883755176770385E-5	0.0	0.0	0.0
4	0.0	1.6883755176770385E-5	0.0	0.0	0.0
5	0.0	3.376751035354077E-5	0.0	1.6883755176770385E-5	0.0
6	0.0	3.376751035354077E-5	0.0	5.065126553031115E-5	0.0
7	0.0	3.376751035354077E-5	0.0	5.065126553031115E-5	0.0
8	0.0	3.376751035354077E-5	0.0	5.065126553031115E-5	0.0
9	0.0	3.376751035354077E-5	0.0	5.065126553031115E-5	0.0
10-11	0.0	3.376751035354077E-5	0.0	6.753502070708154E-5	0.0
12-13	0.0	5.065126553031116E-5	0.0	8.441877588385192E-5	0.0
14-15	0.0	6.753502070708154E-5	0.0	1.181862862373927E-4	0.0
16-17	0.0	6.753502070708154E-5	0.0	1.6883755176770384E-4	0.0
18-19	0.0	8.441877588385192E-5	0.0	1.9416318453285942E-4	0.0
20-21	0.0	1.013025310606223E-4	0.0	2.8702383800509654E-4	0.0
22-23	0.0	1.097444086490075E-4	0.0	6.753502070708154E-4	0.0
24-25	0.0	1.1818628623739268E-4	0.0	0.0011480953520203862	0.0
26-27	0.0	1.1818628623739268E-4	0.0	0.001941631845328594	0.0
28-29	0.0	1.3507004141416308E-4	0.0	0.00482875398055633	0.0
30-31	0.0	1.4351191900254827E-4	0.0	0.015372659088449436	0.0
32-33	0.0	1.5195379659093346E-4	0.0	0.04024243046383221	0.0
34-35	0.0	1.5195379659093346E-4	0.0	0.07763150630279023	0.0
36-37	0.0	1.6883755176770384E-4	0.0	0.13409078361391039	0.0
38-39	0.0	2.3637257247478537E-4	0.0	0.2107092646060944	0.0
40-41	0.0	2.3637257247478537E-4	0.0	0.28798621205017244	0.0
42-43	1.6883755176770385E-5	2.3637257247478537E-4	0.0	0.36468911181824026	0.0
44-45	1.6883755176770385E-5	2.448144500631706E-4	0.0	0.4590270938684448	0.0
46-47	1.6883755176770385E-5	3.039075931818669E-4	0.0	0.572469044901165	0.0
48-49	1.6883755176770385E-5	3.039075931818669E-4	0.0	0.6895663289296561	0.0
50-51	1.6883755176770385E-5	3.039075931818669E-4	0.0	0.8056168201371873	0.0
52-53	1.6883755176770385E-5	3.207913483586373E-4	0.0	0.9178009314092381	0.0
54-55	1.6883755176770385E-5	3.376751035354077E-4	0.0	1.042546556532806	0.0
56-57	1.6883755176770385E-5	3.8832636906571883E-4	0.0	1.1883884337497486	0.0
58-59	1.6883755176770385E-5	4.052101242424892E-4	0.0	1.3428241423516674	0.0
60-61	1.6883755176770385E-5	4.052101242424892E-4	0.0	1.5008814164390034	0.0
62-63	1.6883755176770385E-5	4.3897763459603E-4	0.0	1.6574613619483718	0.0
64-65	1.6883755176770385E-5	4.5586138977280036E-4	0.0	1.8221539518201786	0.0
66-67	1.6883755176770385E-5	4.896289001263412E-4	0.0	2.009985728161749	0.0
68-69	1.6883755176770385E-5	6.078151863637338E-4	0.0	2.2114511368085616	0.0
70-71	1.6883755176770385E-5	6.078151863637338E-4	0.0	2.4286606471577126	0.0
72-73	1.6883755176770385E-5	6.078151863637338E-4	0.0	2.648200115721258	0.0
74-75	1.6883755176770385E-5	6.078151863637338E-4	0.0	2.8695377042111296	0.0
76-77	2.532563276515558E-5	6.078151863637338E-4	0.0	3.1068304413430488	0.0
78-79	3.376751035354077E-5	6.078151863637338E-4	0.0	3.3705209296938485	0.0
80-81	3.376751035354077E-5	6.415826967172746E-4	0.0	3.6505886605661155	0.0
82-83	3.376751035354077E-5	6.669083294824302E-4	0.0	3.939773619233839	0.0
84-85	3.376751035354077E-5	6.753502070708154E-4	0.0	4.224180475186536	0.0
86-87	3.376751035354077E-5	6.753502070708154E-4	0.0	4.528138719633933	0.0
88	3.376751035354077E-5	6.753502070708154E-4	0.0	4.773375263576523	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5530	0.0	44.637005	1
GTATCAA	9430	0.0	34.60259	1
TCAACGC	9940	0.0	31.736593	4
ATCAACG	10065	0.0	31.342443	3
CAACGCA	10650	0.0	29.532782	5
AACGCAG	11350	0.0	27.747404	6
TATCAAC	11865	0.0	26.904577	2
ACGCAGA	13280	0.0	23.25411	7
GTACATG	15245	0.0	23.007648	1
CGCAGAG	13490	0.0	22.439148	8
TACATGG	15175	0.0	21.469833	2
ACATGGG	15855	0.0	19.867018	3
GTGGTAT	3635	0.0	18.108583	1
GCAGAGT	15690	0.0	18.094646	9
CATGGGG	11355	0.0	17.762098	4
GAGTACA	11780	0.0	16.96307	1
AGAGTAC	13270	0.0	16.931408	10-11
CAGAGTA	14875	0.0	16.115704	10-11
TGGTATC	3675	0.0	15.095546	2
CTGTCGC	1395	0.0	14.825678	9
>>END_MODULE