FastQCFastQC Report
Wed 25 May 2016
SRR2049389_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049389_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5922853
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130040.2195563523187221No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129410.21849267574258555No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74240.12534499843234334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95600.055.5699841
GTATCAA165750.037.780741
ATCAACG200300.030.689433
TCAACGC201650.030.5305864
GTGGTAT42900.029.5887221
CAACGCA208250.029.5178555
TATCAAC210550.029.4632572
AACGCAG216200.028.5415556
TGGTATC44400.026.0383952
ACGCAGA248500.024.6615947
CGCAGAG252250.024.0154938
GTACATG147700.021.9628311
GCAGAGT278250.021.48419
GAGTACT195050.020.9344712-13
TACATGG148400.020.331212
CAGAGTA271800.020.01257710-11
ACATGGG149800.019.576493
AGAGTAC255900.019.3261710-11
GTACTTT217200.018.5432114-15
AGTACTT208250.017.77884912-13
Produced by FastQC (version 0.11.5)